ChloroquineV1, Main, Merge, bibRecord, 003473

Cloning and expression of multiple protein kinase C cDNAs

Identifieur interne : 003473 ( Main/Merge ); précédent : 003472; suivant : 003474

Cloning and expression of multiple protein kinase C cDNAs

Auteurs : John L. Knopf [États-Unis] ; Myung-Ho Lee [États-Unis] ; Lisa A. Sultzman [États-Unis] ; Ronald W. Kriz [États-Unis] ; Carson R. Loomis [États-Unis] ; Rodney M. Hewick [États-Unis] ; Robert M. Bell [États-Unis]

Source :

Cell [ 0092-8674 ] ; 1986.

RBID : ISTEX:CB07BCA4532C5BE27D80D584747C4F07D4CC1770

English descriptors

Teeft :
- Amino, Amino acid sequence, Amino acid sequences, Biol, Brain cdna library, Brain protein kinase, Cdna, Cdna clones, Cells transfected, Chem, Clone, Coding region, Cysteine, Cysteine residues, Encode, Ester, Experimental procedures, Expression vector, Guessmer, Hannun, Homogenization buffer, Homology, Kinase, Mrna, Nishizuka, Northern blot analysis, Nucleotide, Oligonucleotide, Pdbu, Peptide, Phorbol, Phorbol ester binding, Phorbol esters, Phosphatidylserine, Phospholipid, Polypeptide, Protein, Protein kinase, Protein kinase activity, Protein kinases, Protein sequences, Receptor, Sequence analysis, Specific binding, Tissue distribution, Transfected, Tryptic, Tyrodes solution.

Abstract

Abstract: Three different protein kinase C related cDNA clones were isolated from a rat brain cDNA library and designated PKC-I, PKC-II, and PKC-III. These each encode very similar, but distinct, polypeptides that contain a region homologous with other protein kinases. COS cells transfected with either PKC-I or PKC-II specifically bind at least 5-fold more 3H-PDBu (phorbol ester) than control cells. An increase in Ca2+, phosphatidylserine, and diacylglycerol/phorbol-ester-dependent protein kinase activity is also observed in COS cells transfected with either PKC-I or PKC-II. The physiological implications of the discovery of three protein-kinase-C-related cDNAs are discussed.

Url:

https://api.istex.fr/ark:/67375/6H6-CBJ5WFP9-L/fulltext.pdf

DOI: 10.1016/0092-8674(86)90874-3

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ISTEX:CB07BCA4532C5BE27D80D584747C4F07D4CC1770

Le document en format XML

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<author><name sortKey="Lee, Myung Ho" sort="Lee, Myung Ho" uniqKey="Lee M" first="Myung-Ho" last="Lee">Myung-Ho Lee</name>
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<author><name sortKey="Sultzman, Lisa A" sort="Sultzman, Lisa A" uniqKey="Sultzman L" first="Lisa A." last="Sultzman">Lisa A. Sultzman</name>
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<author><name sortKey="Kriz, Ronald W" sort="Kriz, Ronald W" uniqKey="Kriz R" first="Ronald W." last="Kriz">Ronald W. Kriz</name>
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<author><name sortKey="Loomis, Carson R" sort="Loomis, Carson R" uniqKey="Loomis C" first="Carson R." last="Loomis">Carson R. Loomis</name>
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<author><name sortKey="Hewick, Rodney M" sort="Hewick, Rodney M" uniqKey="Hewick R" first="Rodney M." last="Hewick">Rodney M. Hewick</name>
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<author><name sortKey="Bell, Robert M" sort="Bell, Robert M" uniqKey="Bell R" first="Robert M." last="Bell">Robert M. Bell</name>
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<author><name sortKey="Hewick, Rodney M" sort="Hewick, Rodney M" uniqKey="Hewick R" first="Rodney M." last="Hewick">Rodney M. Hewick</name>
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<author><name sortKey="Bell, Robert M" sort="Bell, Robert M" uniqKey="Bell R" first="Robert M." last="Bell">Robert M. Bell</name>
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<term>Brain cdna library</term>
<term>Brain protein kinase</term>
<term>Cdna</term>
<term>Cdna clones</term>
<term>Cells transfected</term>
<term>Chem</term>
<term>Clone</term>
<term>Coding region</term>
<term>Cysteine</term>
<term>Cysteine residues</term>
<term>Encode</term>
<term>Ester</term>
<term>Experimental procedures</term>
<term>Expression vector</term>
<term>Guessmer</term>
<term>Hannun</term>
<term>Homogenization buffer</term>
<term>Homology</term>
<term>Kinase</term>
<term>Mrna</term>
<term>Nishizuka</term>
<term>Northern blot analysis</term>
<term>Nucleotide</term>
<term>Oligonucleotide</term>
<term>Pdbu</term>
<term>Peptide</term>
<term>Phorbol</term>
<term>Phorbol ester binding</term>
<term>Phorbol esters</term>
<term>Phosphatidylserine</term>
<term>Phospholipid</term>
<term>Polypeptide</term>
<term>Protein</term>
<term>Protein kinase</term>
<term>Protein kinase activity</term>
<term>Protein kinases</term>
<term>Protein sequences</term>
<term>Receptor</term>
<term>Sequence analysis</term>
<term>Specific binding</term>
<term>Tissue distribution</term>
<term>Transfected</term>
<term>Tryptic</term>
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<front><div type="abstract" xml:lang="en">Abstract: Three different protein kinase C related cDNA clones were isolated from a rat brain cDNA library and designated PKC-I, PKC-II, and PKC-III. These each encode very similar, but distinct, polypeptides that contain a region homologous with other protein kinases. COS cells transfected with either PKC-I or PKC-II specifically bind at least 5-fold more 3H-PDBu (phorbol ester) than control cells. An increase in Ca2+, phosphatidylserine, and diacylglycerol/phorbol-ester-dependent protein kinase activity is also observed in COS cells transfected with either PKC-I or PKC-II. The physiological implications of the discovery of three protein-kinase-C-related cDNAs are discussed.</div>
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Cloning and expression of multiple protein kinase C cDNAs

Cloning and expression of multiple protein kinase C cDNAs

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri