Cloning and expression of multiple protein kinase C cDNAs
Identifieur interne : 002A50 ( Istex/Curation ); précédent : 002A49; suivant : 002A51Cloning and expression of multiple protein kinase C cDNAs
Auteurs : John L. Knopf [États-Unis] ; Myung-Ho Lee [États-Unis] ; Lisa A. Sultzman [États-Unis] ; Ronald W. Kriz [États-Unis] ; Carson R. Loomis [États-Unis] ; Rodney M. Hewick [États-Unis] ; Robert M. Bell [États-Unis]Source :
- Cell [ 0092-8674 ] ; 1986.
English descriptors
- Teeft :
- Amino, Amino acid sequence, Amino acid sequences, Biol, Brain cdna library, Brain protein kinase, Cdna, Cdna clones, Cells transfected, Chem, Clone, Coding region, Cysteine, Cysteine residues, Encode, Ester, Experimental procedures, Expression vector, Guessmer, Hannun, Homogenization buffer, Homology, Kinase, Mrna, Nishizuka, Northern blot analysis, Nucleotide, Oligonucleotide, Pdbu, Peptide, Phorbol, Phorbol ester binding, Phorbol esters, Phosphatidylserine, Phospholipid, Polypeptide, Protein, Protein kinase, Protein kinase activity, Protein kinases, Protein sequences, Receptor, Sequence analysis, Specific binding, Tissue distribution, Transfected, Tryptic, Tyrodes solution.
Abstract
Abstract: Three different protein kinase C related cDNA clones were isolated from a rat brain cDNA library and designated PKC-I, PKC-II, and PKC-III. These each encode very similar, but distinct, polypeptides that contain a region homologous with other protein kinases. COS cells transfected with either PKC-I or PKC-II specifically bind at least 5-fold more 3H-PDBu (phorbol ester) than control cells. An increase in Ca2+, phosphatidylserine, and diacylglycerol/phorbol-ester-dependent protein kinase activity is also observed in COS cells transfected with either PKC-I or PKC-II. The physiological implications of the discovery of three protein-kinase-C-related cDNAs are discussed.
Url:
DOI: 10.1016/0092-8674(86)90874-3
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002A50
Links to Exploration step
ISTEX:CB07BCA4532C5BE27D80D584747C4F07D4CC1770Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Cloning and expression of multiple protein kinase C cDNAs</title>
<author><name sortKey="Knopf, John L" sort="Knopf, John L" uniqKey="Knopf J" first="John L." last="Knopf">John L. Knopf</name>
<affiliation wicri:level="2"><mods:affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Lee, Myung Ho" sort="Lee, Myung Ho" uniqKey="Lee M" first="Myung-Ho" last="Lee">Myung-Ho Lee</name>
<affiliation wicri:level="2"><mods:affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
<wicri:cityArea>The Department of Biochemistry Duke University Medical Center Durham</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Sultzman, Lisa A" sort="Sultzman, Lisa A" uniqKey="Sultzman L" first="Lisa A." last="Sultzman">Lisa A. Sultzman</name>
<affiliation wicri:level="2"><mods:affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Kriz, Ronald W" sort="Kriz, Ronald W" uniqKey="Kriz R" first="Ronald W." last="Kriz">Ronald W. Kriz</name>
<affiliation wicri:level="2"><mods:affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Loomis, Carson R" sort="Loomis, Carson R" uniqKey="Loomis C" first="Carson R." last="Loomis">Carson R. Loomis</name>
<affiliation wicri:level="2"><mods:affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
<wicri:cityArea>The Department of Biochemistry Duke University Medical Center Durham</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Hewick, Rodney M" sort="Hewick, Rodney M" uniqKey="Hewick R" first="Rodney M." last="Hewick">Rodney M. Hewick</name>
<affiliation wicri:level="2"><mods:affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Bell, Robert M" sort="Bell, Robert M" uniqKey="Bell R" first="Robert M." last="Bell">Robert M. Bell</name>
<affiliation wicri:level="2"><mods:affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
<wicri:cityArea>The Department of Biochemistry Duke University Medical Center Durham</wicri:cityArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:CB07BCA4532C5BE27D80D584747C4F07D4CC1770</idno>
<date when="1986" year="1986">1986</date>
<idno type="doi">10.1016/0092-8674(86)90874-3</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-CBJ5WFP9-L/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">002A50</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002A50</idno>
<idno type="wicri:Area/Istex/Curation">002A50</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Cloning and expression of multiple protein kinase C cDNAs</title>
<author><name sortKey="Knopf, John L" sort="Knopf, John L" uniqKey="Knopf J" first="John L." last="Knopf">John L. Knopf</name>
<affiliation wicri:level="2"><mods:affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Lee, Myung Ho" sort="Lee, Myung Ho" uniqKey="Lee M" first="Myung-Ho" last="Lee">Myung-Ho Lee</name>
<affiliation wicri:level="2"><mods:affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
<wicri:cityArea>The Department of Biochemistry Duke University Medical Center Durham</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Sultzman, Lisa A" sort="Sultzman, Lisa A" uniqKey="Sultzman L" first="Lisa A." last="Sultzman">Lisa A. Sultzman</name>
<affiliation wicri:level="2"><mods:affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Kriz, Ronald W" sort="Kriz, Ronald W" uniqKey="Kriz R" first="Ronald W." last="Kriz">Ronald W. Kriz</name>
<affiliation wicri:level="2"><mods:affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Loomis, Carson R" sort="Loomis, Carson R" uniqKey="Loomis C" first="Carson R." last="Loomis">Carson R. Loomis</name>
<affiliation wicri:level="2"><mods:affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
<wicri:cityArea>The Department of Biochemistry Duke University Medical Center Durham</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Hewick, Rodney M" sort="Hewick, Rodney M" uniqKey="Hewick R" first="Rodney M." last="Hewick">Rodney M. Hewick</name>
<affiliation wicri:level="2"><mods:affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Bell, Robert M" sort="Bell, Robert M" uniqKey="Bell R" first="Robert M." last="Bell">Robert M. Bell</name>
<affiliation wicri:level="2"><mods:affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</mods:affiliation>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Caroline du Nord</region>
</placeName>
<wicri:cityArea>The Department of Biochemistry Duke University Medical Center Durham</wicri:cityArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Cell</title>
<title level="j" type="abbrev">CELL</title>
<idno type="ISSN">0092-8674</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1986">1986</date>
<biblScope unit="volume">46</biblScope>
<biblScope unit="issue">4</biblScope>
<biblScope unit="page" from="491">491</biblScope>
<biblScope unit="page" to="502">502</biblScope>
</imprint>
<idno type="ISSN">0092-8674</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0092-8674</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term>
<term>Amino acid sequence</term>
<term>Amino acid sequences</term>
<term>Biol</term>
<term>Brain cdna library</term>
<term>Brain protein kinase</term>
<term>Cdna</term>
<term>Cdna clones</term>
<term>Cells transfected</term>
<term>Chem</term>
<term>Clone</term>
<term>Coding region</term>
<term>Cysteine</term>
<term>Cysteine residues</term>
<term>Encode</term>
<term>Ester</term>
<term>Experimental procedures</term>
<term>Expression vector</term>
<term>Guessmer</term>
<term>Hannun</term>
<term>Homogenization buffer</term>
<term>Homology</term>
<term>Kinase</term>
<term>Mrna</term>
<term>Nishizuka</term>
<term>Northern blot analysis</term>
<term>Nucleotide</term>
<term>Oligonucleotide</term>
<term>Pdbu</term>
<term>Peptide</term>
<term>Phorbol</term>
<term>Phorbol ester binding</term>
<term>Phorbol esters</term>
<term>Phosphatidylserine</term>
<term>Phospholipid</term>
<term>Polypeptide</term>
<term>Protein</term>
<term>Protein kinase</term>
<term>Protein kinase activity</term>
<term>Protein kinases</term>
<term>Protein sequences</term>
<term>Receptor</term>
<term>Sequence analysis</term>
<term>Specific binding</term>
<term>Tissue distribution</term>
<term>Transfected</term>
<term>Tryptic</term>
<term>Tyrodes solution</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Three different protein kinase C related cDNA clones were isolated from a rat brain cDNA library and designated PKC-I, PKC-II, and PKC-III. These each encode very similar, but distinct, polypeptides that contain a region homologous with other protein kinases. COS cells transfected with either PKC-I or PKC-II specifically bind at least 5-fold more 3H-PDBu (phorbol ester) than control cells. An increase in Ca2+, phosphatidylserine, and diacylglycerol/phorbol-ester-dependent protein kinase activity is also observed in COS cells transfected with either PKC-I or PKC-II. The physiological implications of the discovery of three protein-kinase-C-related cDNAs are discussed.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002A50 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 002A50 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= ChloroquineV1 |flux= Istex |étape= Curation |type= RBID |clé= ISTEX:CB07BCA4532C5BE27D80D584747C4F07D4CC1770 |texte= Cloning and expression of multiple protein kinase C cDNAs }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |