Cloning and expression of multiple protein kinase C cDNAs
Identifieur interne : 002A50 ( Istex/Corpus ); précédent : 002A49; suivant : 002A51Cloning and expression of multiple protein kinase C cDNAs
Auteurs : John L. Knopf ; Myung-Ho Lee ; Lisa A. Sultzman ; Ronald W. Kriz ; Carson R. Loomis ; Rodney M. Hewick ; Robert M. BellSource :
- Cell [ 0092-8674 ] ; 1986.
English descriptors
- Teeft :
- Amino, Amino acid sequence, Amino acid sequences, Biol, Brain cdna library, Brain protein kinase, Cdna, Cdna clones, Cells transfected, Chem, Clone, Coding region, Cysteine, Cysteine residues, Encode, Ester, Experimental procedures, Expression vector, Guessmer, Hannun, Homogenization buffer, Homology, Kinase, Mrna, Nishizuka, Northern blot analysis, Nucleotide, Oligonucleotide, Pdbu, Peptide, Phorbol, Phorbol ester binding, Phorbol esters, Phosphatidylserine, Phospholipid, Polypeptide, Protein, Protein kinase, Protein kinase activity, Protein kinases, Protein sequences, Receptor, Sequence analysis, Specific binding, Tissue distribution, Transfected, Tryptic, Tyrodes solution.
Abstract
Abstract: Three different protein kinase C related cDNA clones were isolated from a rat brain cDNA library and designated PKC-I, PKC-II, and PKC-III. These each encode very similar, but distinct, polypeptides that contain a region homologous with other protein kinases. COS cells transfected with either PKC-I or PKC-II specifically bind at least 5-fold more 3H-PDBu (phorbol ester) than control cells. An increase in Ca2+, phosphatidylserine, and diacylglycerol/phorbol-ester-dependent protein kinase activity is also observed in COS cells transfected with either PKC-I or PKC-II. The physiological implications of the discovery of three protein-kinase-C-related cDNAs are discussed.
Url:
DOI: 10.1016/0092-8674(86)90874-3
Links to Exploration step
ISTEX:CB07BCA4532C5BE27D80D584747C4F07D4CC1770Le document en format XML
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Knopf</name><affiliation><mods:affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</mods:affiliation></affiliation></author><author><name sortKey="Lee, Myung Ho" sort="Lee, Myung Ho" uniqKey="Lee M" first="Myung-Ho" last="Lee">Myung-Ho Lee</name><affiliation><mods:affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</mods:affiliation></affiliation></author><author><name sortKey="Sultzman, Lisa A" sort="Sultzman, Lisa A" uniqKey="Sultzman L" first="Lisa A." last="Sultzman">Lisa A. Sultzman</name><affiliation><mods:affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</mods:affiliation></affiliation></author><author><name sortKey="Kriz, Ronald W" sort="Kriz, Ronald W" uniqKey="Kriz R" first="Ronald W." last="Kriz">Ronald W. Kriz</name><affiliation><mods:affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</mods:affiliation></affiliation></author><author><name sortKey="Loomis, Carson R" sort="Loomis, Carson R" uniqKey="Loomis C" first="Carson R." last="Loomis">Carson R. Loomis</name><affiliation><mods:affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</mods:affiliation></affiliation></author><author><name sortKey="Hewick, Rodney M" sort="Hewick, Rodney M" uniqKey="Hewick R" first="Rodney M." last="Hewick">Rodney M. Hewick</name><affiliation><mods:affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</mods:affiliation></affiliation></author><author><name sortKey="Bell, Robert M" sort="Bell, Robert M" uniqKey="Bell R" first="Robert M." last="Bell">Robert M. Bell</name><affiliation><mods:affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="ISSN">0092-8674</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1986">1986</date><biblScope unit="volume">46</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="491">491</biblScope><biblScope unit="page" to="502">502</biblScope></imprint><idno type="ISSN">0092-8674</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0092-8674</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid sequence</term><term>Amino acid sequences</term><term>Biol</term><term>Brain cdna library</term><term>Brain protein kinase</term><term>Cdna</term><term>Cdna clones</term><term>Cells transfected</term><term>Chem</term><term>Clone</term><term>Coding region</term><term>Cysteine</term><term>Cysteine residues</term><term>Encode</term><term>Ester</term><term>Experimental procedures</term><term>Expression vector</term><term>Guessmer</term><term>Hannun</term><term>Homogenization buffer</term><term>Homology</term><term>Kinase</term><term>Mrna</term><term>Nishizuka</term><term>Northern blot analysis</term><term>Nucleotide</term><term>Oligonucleotide</term><term>Pdbu</term><term>Peptide</term><term>Phorbol</term><term>Phorbol ester binding</term><term>Phorbol esters</term><term>Phosphatidylserine</term><term>Phospholipid</term><term>Polypeptide</term><term>Protein</term><term>Protein kinase</term><term>Protein kinase activity</term><term>Protein kinases</term><term>Protein sequences</term><term>Receptor</term><term>Sequence analysis</term><term>Specific binding</term><term>Tissue distribution</term><term>Transfected</term><term>Tryptic</term><term>Tyrodes solution</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Three different protein kinase C related cDNA clones were isolated from a rat brain cDNA library and designated PKC-I, PKC-II, and PKC-III. These each encode very similar, but distinct, polypeptides that contain a region homologous with other protein kinases. COS cells transfected with either PKC-I or PKC-II specifically bind at least 5-fold more 3H-PDBu (phorbol ester) than control cells. An increase in Ca2+, phosphatidylserine, and diacylglycerol/phorbol-ester-dependent protein kinase activity is also observed in COS cells transfected with either PKC-I or PKC-II. 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Knopf</name><affiliations><json:string>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</json:string></affiliations></json:item><json:item><name>Myung-Ho Lee</name><affiliations><json:string>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</json:string></affiliations></json:item><json:item><name>Lisa A. Sultzman</name><affiliations><json:string>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</json:string></affiliations></json:item><json:item><name>Ronald W. Kriz</name><affiliations><json:string>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</json:string></affiliations></json:item><json:item><name>Carson R. Loomis</name><affiliations><json:string>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</json:string></affiliations></json:item><json:item><name>Rodney M. Hewick</name><affiliations><json:string>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</json:string></affiliations></json:item><json:item><name>Robert M. Bell</name><affiliations><json:string>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-CBJ5WFP9-L</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Three different protein kinase C related cDNA clones were isolated from a rat brain cDNA library and designated PKC-I, PKC-II, and PKC-III. These each encode very similar, but distinct, polypeptides that contain a region homologous with other protein kinases. COS cells transfected with either PKC-I or PKC-II specifically bind at least 5-fold more 3H-PDBu (phorbol ester) than control cells. An increase in Ca2+, phosphatidylserine, and diacylglycerol/phorbol-ester-dependent protein kinase activity is also observed in COS cells transfected with either PKC-I or PKC-II. The physiological implications of the discovery of three protein-kinase-C-related cDNAs are discussed.</abstract><qualityIndicators><score>8.128</score><pdfWordCount>6157</pdfWordCount><pdfCharCount>43262</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>12</pdfPageCount><pdfPageSize>612 x 828 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>94</abstractWordCount><abstractCharCount>684</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Cloning and expression of multiple protein kinase C cDNAs</title><pmid><json:string>3755379</json:string></pmid><pii><json:string>0092-8674(86)90874-3</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. Sci. 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Biol</json:string><json:string>Rick Boulay</json:string><json:string>J. Cell</json:string><json:string>Yusuf Hannun</json:string><json:string>John Brown</json:string><json:string>Lisa A. Sultzman</json:string><json:string>C. Catalytic</json:string><json:string>Carson Ft</json:string><json:string>Simon Jones</json:string><json:string>O.InM</json:string><json:string>Nishizuka</json:string><json:string>N.A. Kinases</json:string><json:string>Charles River</json:string><json:string>Phorbol</json:string><json:string>J. Mol</json:string><json:string>Ken Jacobs</json:string><json:string>Spring Harbor</json:string><json:string>R. Sot</json:string><json:string>C. Meth</json:string><json:string>Ronald W. Kriz</json:string><json:string>Robert Ralston</json:string><json:string>Blumberg</json:string><json:string>Robert Kamen</json:string><json:string>Rodney M. Hewick</json:string><json:string>C. Mild</json:string></persName><placeName><json:string>Utrecht</json:string><json:string>COS</json:string><json:string>Takayama</json:string><json:string>Specificity</json:string><json:string>Tokyo</json:string><json:string>York</json:string><json:string>Cambridge</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Hannun et al., 1985</json:string><json:string>Nishizuka, 1984</json:string><json:string>Wang et al., 1965</json:string><json:string>Sudhof et al., 1985</json:string><json:string>Greene et al., 1986</json:string><json:string>Poncz et al. 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(1984)</json:string><json:string>Luthman and Magnusson, 1983</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-CBJ5WFP9-L</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - cell biology</json:string><json:string>2 - biochemistry & molecular biology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - developmental biology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - General Biochemistry, Genetics and Molecular Biology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. 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type="first">Myung-Ho</forename><surname>Lee</surname></persName><affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Lisa A.</forename><surname>Sultzman</surname></persName><affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Ronald W.</forename><surname>Kriz</surname></persName><affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Carson R.</forename><surname>Loomis</surname></persName><affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Rodney M.</forename><surname>Hewick</surname></persName><affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</affiliation></author><author xml:id="author-0006"><persName><forename type="first">Robert M.</forename><surname>Bell</surname></persName><affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</affiliation></author><idno type="istex">CB07BCA4532C5BE27D80D584747C4F07D4CC1770</idno><idno type="ark">ark:/67375/6H6-CBJ5WFP9-L</idno><idno type="DOI">10.1016/0092-8674(86)90874-3</idno><idno type="PII">0092-8674(86)90874-3</idno></analytic><monogr><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="pISSN">0092-8674</idno><idno type="PII">S0092-8674(00)X0324-8</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1986"></date><biblScope unit="volume">46</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="491">491</biblScope><biblScope unit="page" to="502">502</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1986</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Three different protein kinase C related cDNA clones were isolated from a rat brain cDNA library and designated PKC-I, PKC-II, and PKC-III. These each encode very similar, but distinct, polypeptides that contain a region homologous with other protein kinases. COS cells transfected with either PKC-I or PKC-II specifically bind at least 5-fold more 3H-PDBu (phorbol ester) than control cells. An increase in Ca2+, phosphatidylserine, and diacylglycerol/phorbol-ester-dependent protein kinase activity is also observed in COS cells transfected with either PKC-I or PKC-II. The physiological implications of the discovery of three protein-kinase-C-related cDNAs are discussed.</p></abstract></profileDesc><revisionDesc><change when="1986">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-CBJ5WFP9-L/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>CELL</jid><aid>86908743</aid><ce:pii>0092-8674(86)90874-3</ce:pii><ce:doi>10.1016/0092-8674(86)90874-3</ce:doi><ce:copyright type="unknown" year="1986"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Article</ce:textfn></ce:dochead><ce:title>Cloning and expression of multiple protein kinase C cDNAs</ce:title><ce:author-group><ce:author><ce:given-name>John L.</ce:given-name><ce:surname>Knopf</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>★</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Myung-Ho</ce:given-name><ce:surname>Lee</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Lisa A.</ce:given-name><ce:surname>Sultzman</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>★</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Ronald W.</ce:given-name><ce:surname>Kriz</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>★</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Carson R.</ce:given-name><ce:surname>Loomis</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Rodney M.</ce:given-name><ce:surname>Hewick</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>★</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Robert M.</ce:given-name><ce:surname>Bell</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="1" month="7" year="1986"></ce:date-received><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Three different protein kinase C related cDNA clones were isolated from a rat brain cDNA library and designated PKC-I, PKC-II, and PKC-III. These each encode very similar, but distinct, polypeptides that contain a region homologous with other protein kinases. COS cells transfected with either PKC-I or PKC-II specifically bind at least 5-fold more <ce:sup loc="pre">3</ce:sup>H-PDBu (phorbol ester) than control cells. An increase in Ca<ce:sup>2+</ce:sup>, phosphatidylserine, and diacylglycerol/phorbol-ester-dependent protein kinase activity is also observed in COS cells transfected with either PKC-I or PKC-II. The physiological implications of the discovery of three protein-kinase-C-related cDNAs are discussed.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Cloning and expression of multiple protein kinase C cDNAs</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Cloning and expression of multiple protein kinase C cDNAs</title></titleInfo><name type="personal"><namePart type="given">John L.</namePart><namePart type="family">Knopf</namePart><affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Myung-Ho</namePart><namePart type="family">Lee</namePart><affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lisa A.</namePart><namePart type="family">Sultzman</namePart><affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ronald W.</namePart><namePart type="family">Kriz</namePart><affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Carson R.</namePart><namePart type="family">Loomis</namePart><affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rodney M.</namePart><namePart type="family">Hewick</namePart><affiliation>Genetics Institute, Inc. 87 Cambridge Park Drive Cambridge, Massachusetts 02140 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Robert M.</namePart><namePart type="family">Bell</namePart><affiliation>The Department of Biochemistry Duke University Medical Center Durham, North Carolina 27710 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1986</dateIssued><copyrightDate encoding="w3cdtf">1986</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Three different protein kinase C related cDNA clones were isolated from a rat brain cDNA library and designated PKC-I, PKC-II, and PKC-III. These each encode very similar, but distinct, polypeptides that contain a region homologous with other protein kinases. COS cells transfected with either PKC-I or PKC-II specifically bind at least 5-fold more 3H-PDBu (phorbol ester) than control cells. An increase in Ca2+, phosphatidylserine, and diacylglycerol/phorbol-ester-dependent protein kinase activity is also observed in COS cells transfected with either PKC-I or PKC-II. The physiological implications of the discovery of three protein-kinase-C-related cDNAs are discussed.</abstract><note type="content">Section title: Article</note><relatedItem type="host"><titleInfo><title>Cell</title></titleInfo><titleInfo type="abbreviated"><title>CELL</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1986</dateIssued></originInfo><identifier type="ISSN">0092-8674</identifier><identifier type="PII">S0092-8674(00)X0324-8</identifier><part><date>1986</date><detail type="volume"><number>46</number><caption>vol.</caption></detail><detail type="issue"><number>4</number><caption>no.</caption></detail><extent unit="issue-pages"><start>489</start><end>639</end></extent><extent unit="pages"><start>491</start><end>502</end></extent></part></relatedItem><identifier type="istex">CB07BCA4532C5BE27D80D584747C4F07D4CC1770</identifier><identifier type="ark">ark:/67375/6H6-CBJ5WFP9-L</identifier><identifier type="DOI">10.1016/0092-8674(86)90874-3</identifier><identifier type="PII">0092-8674(86)90874-3</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-CBJ5WFP9-L/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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