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A unique tumor antigen produced by a single amino acid substitution

Identifieur interne : 002B24 ( Main/Exploration ); précédent : 002B23; suivant : 002B25

A unique tumor antigen produced by a single amino acid substitution

Auteurs : Paul A. Monach [États-Unis] ; Stephen C. Meredith [États-Unis] ; Christopher T. Siegel [États-Unis] ; Hans Schreiber [États-Unis]

Source :

Immunity [ 1074-7613 ] ; 1995.

RBID : ISTEX:9102E8296D3E74AB60AC334F7E1C2F04827972F9

English descriptors

Teeft :
- Acad, Accn, Amino, Amino acid sequence, Amino acids, Antigen, Antigenic, Assay, Autologous, Autologous fibroblasts, Cancer cells, Cdna, Cdna clones, Cell clone, Cell clones, Cell equivalents, Cell hybridoma, Cell lines, Cells transfected, Chloroquine, Clone, Coding sequences, Cytolytic, Cytotoxic, Equal volume, Error bars show, Fibroblast, Fitch, Flow cytometry, Gibco, Histidine, Histocompatibility, Hybridoma, Hybridoma stimulation assay, Immune, Immunol, Koeppen, Linear gradient, Lymph, Lymph node cells, Lymphocyte, Lysate, Lysates, Major histocompatibility, Monoclonal antibodies, Monoclonal antibody, Mouse, Murine, Mutant, Mutant proteins, Mutation, Nacl, Naive mice, National institutes, Natl, Nitrocellulose, Node, Open circle, Open square, Opposite flanks, Peptide, Plasmid, Primer, Pro4l, Proc, Recombinant, Ribosomal, Ribosomal protein, Ribosomal proteins, Schreiber, Scid, Scid mice, Sequencing, Spleen, Spleen cells, Supernatant, Syngeneic, Syngeneic spleen cells, Synthetic peptides, Thymidine, Thymidine incorporation assay, Transfected, Transplantation, Triplicate, Triplicate wells, Tumor, Tumor antigens, Tumor cell lysate, Tumor cell lysates, Tumor cells, Tumor growth, Unique antigen, Unique antigens, Unique tumor antigen, Unique tumor antigens.

Abstract

Abstract: Mice immunized against a cancer recognize antigens unique to that cancer, but the molecular structures of such antigens are unknown. We Isolated CD4+ T cell clones recognizing an antigen uniquely expressed on the UV-induced tumor 6132A; some clones inhibited the growth of tumors bearing the specific antigen. A T cell hybridoma was used to purify this antigen from nuclear extracts by RP-HPLC and SDS-PAGE using T cell immunoblot assays. A partial amino acid sequence was nearly identical to a sequence in ribosomal protein L9. The cDNA sequence of L9 from 6132A PRO cells differed from the normal sequence at one nucleotide; this mutation encoded histidine instead of leucine at position 47. A synthetic peptide containing this mutation was over 1000-fold more stimulatory of T cells than was the wild-type peptide. These results indicate that this unique tumor antigen is derived from a single amino acid substitution in a cellular protein.

Url:

https://api.istex.fr/ark:/67375/6H6-5HZPSK8J-F/fulltext.pdf

DOI: 10.1016/1074-7613(95)90078-0

Affiliations:

États-Unis

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Le document en format XML

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<term>Antigen</term>
<term>Antigenic</term>
<term>Assay</term>
<term>Autologous</term>
<term>Autologous fibroblasts</term>
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<term>Cdna clones</term>
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<term>Cell hybridoma</term>
<term>Cell lines</term>
<term>Cells transfected</term>
<term>Chloroquine</term>
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<term>Cytotoxic</term>
<term>Equal volume</term>
<term>Error bars show</term>
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<term>Ribosomal protein</term>
<term>Ribosomal proteins</term>
<term>Schreiber</term>
<term>Scid</term>
<term>Scid mice</term>
<term>Sequencing</term>
<term>Spleen</term>
<term>Spleen cells</term>
<term>Supernatant</term>
<term>Syngeneic</term>
<term>Syngeneic spleen cells</term>
<term>Synthetic peptides</term>
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<term>Thymidine incorporation assay</term>
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<term>Transplantation</term>
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<front><div type="abstract" xml:lang="en">Abstract: Mice immunized against a cancer recognize antigens unique to that cancer, but the molecular structures of such antigens are unknown. We Isolated CD4+ T cell clones recognizing an antigen uniquely expressed on the UV-induced tumor 6132A; some clones inhibited the growth of tumors bearing the specific antigen. A T cell hybridoma was used to purify this antigen from nuclear extracts by RP-HPLC and SDS-PAGE using T cell immunoblot assays. A partial amino acid sequence was nearly identical to a sequence in ribosomal protein L9. The cDNA sequence of L9 from 6132A PRO cells differed from the normal sequence at one nucleotide; this mutation encoded histidine instead of leucine at position 47. A synthetic peptide containing this mutation was over 1000-fold more stimulatory of T cells than was the wild-type peptide. These results indicate that this unique tumor antigen is derived from a single amino acid substitution in a cellular protein.</div>
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A unique tumor antigen produced by a single amino acid substitution

A unique tumor antigen produced by a single amino acid substitution

Source :

English descriptors

Abstract

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