A unique tumor antigen produced by a single amino acid substitution
Identifieur interne : 001E77 ( Istex/Corpus ); précédent : 001E76; suivant : 001E78A unique tumor antigen produced by a single amino acid substitution
Auteurs : Paul A. Monach ; Stephen C. Meredith ; Christopher T. Siegel ; Hans SchreiberSource :
- Immunity [ 1074-7613 ] ; 1995.
English descriptors
- Teeft :
- Acad, Accn, Amino, Amino acid sequence, Amino acids, Antigen, Antigenic, Assay, Autologous, Autologous fibroblasts, Cancer cells, Cdna, Cdna clones, Cell clone, Cell clones, Cell equivalents, Cell hybridoma, Cell lines, Cells transfected, Chloroquine, Clone, Coding sequences, Cytolytic, Cytotoxic, Equal volume, Error bars show, Fibroblast, Fitch, Flow cytometry, Gibco, Histidine, Histocompatibility, Hybridoma, Hybridoma stimulation assay, Immune, Immunol, Koeppen, Linear gradient, Lymph, Lymph node cells, Lymphocyte, Lysate, Lysates, Major histocompatibility, Monoclonal antibodies, Monoclonal antibody, Mouse, Murine, Mutant, Mutant proteins, Mutation, Nacl, Naive mice, National institutes, Natl, Nitrocellulose, Node, Open circle, Open square, Opposite flanks, Peptide, Plasmid, Primer, Pro4l, Proc, Recombinant, Ribosomal, Ribosomal protein, Ribosomal proteins, Schreiber, Scid, Scid mice, Sequencing, Spleen, Spleen cells, Supernatant, Syngeneic, Syngeneic spleen cells, Synthetic peptides, Thymidine, Thymidine incorporation assay, Transfected, Transplantation, Triplicate, Triplicate wells, Tumor, Tumor antigens, Tumor cell lysate, Tumor cell lysates, Tumor cells, Tumor growth, Unique antigen, Unique antigens, Unique tumor antigen, Unique tumor antigens.
Abstract
Abstract: Mice immunized against a cancer recognize antigens unique to that cancer, but the molecular structures of such antigens are unknown. We Isolated CD4+ T cell clones recognizing an antigen uniquely expressed on the UV-induced tumor 6132A; some clones inhibited the growth of tumors bearing the specific antigen. A T cell hybridoma was used to purify this antigen from nuclear extracts by RP-HPLC and SDS-PAGE using T cell immunoblot assays. A partial amino acid sequence was nearly identical to a sequence in ribosomal protein L9. The cDNA sequence of L9 from 6132A PRO cells differed from the normal sequence at one nucleotide; this mutation encoded histidine instead of leucine at position 47. A synthetic peptide containing this mutation was over 1000-fold more stimulatory of T cells than was the wild-type peptide. These results indicate that this unique tumor antigen is derived from a single amino acid substitution in a cellular protein.
Url:
DOI: 10.1016/1074-7613(95)90078-0
Links to Exploration step
ISTEX:9102E8296D3E74AB60AC334F7E1C2F04827972F9Le document en format XML
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Monach</name><affiliations><json:string>Department of Pathology The University of Chicago Chicago, Illinois 60637, USA</json:string></affiliations></json:item><json:item><name>Stephen C. Meredith</name><affiliations><json:string>Department of Pathology The University of Chicago Chicago, Illinois 60637, USA</json:string></affiliations></json:item><json:item><name>Christopher T.Siegel</name><affiliations><json:string>Department of Pathology The University of Chicago Chicago, Illinois 60637, USA</json:string></affiliations></json:item><json:item><name>Hans Schreiber</name><affiliations><json:string>Department of Pathology The University of Chicago Chicago, Illinois 60637, USA</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-5HZPSK8J-F</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Mice immunized against a cancer recognize antigens unique to that cancer, but the molecular structures of such antigens are unknown. We Isolated CD4+ T cell clones recognizing an antigen uniquely expressed on the UV-induced tumor 6132A; some clones inhibited the growth of tumors bearing the specific antigen. A T cell hybridoma was used to purify this antigen from nuclear extracts by RP-HPLC and SDS-PAGE using T cell immunoblot assays. A partial amino acid sequence was nearly identical to a sequence in ribosomal protein L9. The cDNA sequence of L9 from 6132A PRO cells differed from the normal sequence at one nucleotide; this mutation encoded histidine instead of leucine at position 47. A synthetic peptide containing this mutation was over 1000-fold more stimulatory of T cells than was the wild-type peptide. These results indicate that this unique tumor antigen is derived from a single amino acid substitution in a cellular protein.</abstract><qualityIndicators><score>8.812</score><pdfWordCount>9969</pdfWordCount><pdfCharCount>66123</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>15</pdfPageCount><pdfPageSize>576 x 828 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>151</abstractWordCount><abstractCharCount>953</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>A unique tumor antigen produced by a single amino acid substitution</title><pmid><json:string>7600302</json:string></pmid><pii><json:string>1074-7613(95)90078-0</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. Sci., USA</title><language><json:string>unknown</json:string></language><volume>91</volume><pages><first>3515</first><last>3519</last></pages></serie><host><title>Immunity</title><language><json:string>unknown</json:string></language><publicationDate>1995</publicationDate><issn><json:string>1074-7613</json:string></issn><pii><json:string>S1074-7613(00)X0113-6</json:string></pii><volume>2</volume><issue>1</issue><pages><first>45</first><last>59</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>2000</json:string><json:string>8132</json:string><json:string>14-4-48</json:string><json:string>1995</json:string><json:string>1591</json:string><json:string>10-2-16</json:string><json:string>6132</json:string></date><geogName></geogName><orgName><json:string>Molecular Research Center</json:string><json:string>Genentech</json:string><json:string>University of Illinois</json:string><json:string>National Institutes of Health</json:string><json:string>University of Chicago</json:string><json:string>United States Biochemical</json:string><json:string>Frederick Cancer Research Facility</json:string><json:string>National Institutes of Health</json:string></orgName><orgName_funder><json:string>National Institutes of Health</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Christopher T. Siegel</json:string><json:string>H. Auer</json:string><json:string>J. Miller</json:string><json:string>L. Chan</json:string><json:string>J. Urban</json:string><json:string>T. Wong</json:string><json:string>T. Gajewski</json:string><json:string>Jackson Laboratory</json:string><json:string>A. Houghton</json:string><json:string>K. Schreiber</json:string><json:string>J. Bluestone</json:string><json:string>D. Rowley</json:string><json:string>K. Bigelow</json:string><json:string>S. Linn</json:string><json:string>Elmer Cetus</json:string><json:string>Stephen C. Meredith</json:string><json:string>S. Pierce</json:string><json:string>A. Sant</json:string><json:string>Bands</json:string><json:string>S. Bohlander</json:string><json:string>G. Bowie</json:string><json:string>I. Wool</json:string><json:string>J. Katz</json:string><json:string>F. Fitch</json:string></persName><placeName><json:string>HEL</json:string><json:string>Antigenicity</json:string><json:string>Chicago</json:string><json:string>Cambridge</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Rudensky et al., 1991</json:string><json:string>Mosmann et al., 1986</json:string><json:string>Weisburger and Williams, 1981</json:string><json:string>Gajewski et al., 1969</json:string><json:string>Schagger and von Jagow, 1987</json:string><json:string>Lee et al., 1966</json:string><json:string>Ward et al., 1969</json:string><json:string>Rohrer et al., 1994</json:string><json:string>Allen and Unanue, 1964</json:string><json:string>Ohara and Paul, 1985</json:string><json:string>Oi et al., 1978</json:string><json:string>Ozato et al., 1960</json:string><json:string>Gaugler et al., 1994</json:string><json:string>Philipps et al., 1965</json:string><json:string>Teng et al., 1991</json:string><json:string>Sibille et al., 1990</json:string><json:string>Ortega et al., 1984</json:string><json:string>Cox et al., 1994</json:string><json:string>Leo et al., 1987</json:string><json:string>Mandelboim et al., 1994</json:string><json:string>Hori et al., 1993</json:string><json:string>Srivastava and Old, 1966</json:string><json:string>Wilde and Fitch, 1984</json:string><json:string>Lebkowski et al., 1985</json:string><json:string>Fleischer and Schrezenmeier, 1966</json:string><json:string>DeKruyff et al., 1966</json:string><json:string>Fujiwara et al., 1964</json:string><json:string>Gillis and Smith, 1977</json:string><json:string>Cohen et al., 1994</json:string><json:string>Noguchi et al., 1994</json:string><json:string>Bakker et al., 1994</json:string><json:string>Ward et al., 1990</json:string><json:string>Ltk- cells transfected with I-A’ and genomic Ii; Peterson and Miller, 1992</json:string><json:string>Hunt et al., 1992</json:string><json:string>Joyce et al., 1994</json:string><json:string>Mosmann, 1983</json:string><json:string>Urban et al., 1962</json:string><json:string>Chou and Shu, 1967</json:string><json:string>Sallustio and Stanley, 1990</json:string><json:string>Ward et al., 1989</json:string><json:string>Chan et al., 1994</json:string><json:string>Carbone et al., 1991</json:string><json:string>Jardetzky et al., 1991</json:string><json:string>Chicz et al., 1992</json:string><json:string>Allen and Unanue, 1984</json:string><json:string>Mosmann et al., 1966</json:string><json:string>Brichard et al., 1993</json:string><json:string>Prehn and Main, 1957</json:string><json:string>Buelow et al., 1992</json:string><json:string>Schreiber et al., 1985</json:string><json:string>Greenberg et al., 1961</json:string><json:string>Ozawa et al., 1967</json:string><json:string>p&XV-Ea and pcEXV-EPk; Miller and Germain, 1986</json:string><json:string>Gavin et al., 1993</json:string><json:string>Kuhnlein et al., 1976</json:string><json:string>Young and Lamb, 1966</json:string><json:string>Rosenstein et al., 1964</json:string><json:string>Van der Bruggen et al., 1991</json:string><json:string>for review see Naparstek and Plotz, 1993</json:string><json:string>Ziemiecki et al., 1999</json:string><json:string>Udono et al., 1969</json:string><json:string>Brash and Haseltine, 1982</json:string><json:string>Heinrikson and Meredith, 1984</json:string><json:string>Sambrook et al., 1989</json:string><json:string>S. Linn et al.</json:string><json:string>Goldsby et al., 1977</json:string><json:string>Koeppen et al., 1993a</json:string><json:string>Hickling et al., 1992</json:string><json:string>Greenberg et al., 1965</json:string><json:string>Urban et al., 1982</json:string><json:string>Lurquin et al., 1989</json:string><json:string>Suzuki et al., 1990</json:string><json:string>Kawakami et al., 1994</json:string><json:string>Herman et al., 1991</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-5HZPSK8J-F</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - immunology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - immunology</json:string></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Infectious Diseases</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Immunology</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Immunology and Allergy</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - biotechnologie</json:string></inist></categories><publicationDate>1995</publicationDate><copyrightDate>1995</copyrightDate><doi><json:string>10.1016/1074-7613(95)90078-0</json:string></doi><id>9102E8296D3E74AB60AC334F7E1C2F04827972F9</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5HZPSK8J-F/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5HZPSK8J-F/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-5HZPSK8J-F/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">A unique tumor antigen produced by a single amino acid substitution</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1995</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">A unique tumor antigen produced by a single amino acid substitution</title><author xml:id="author-0000"><persName><forename type="first">Paul A.</forename><surname>Monach</surname></persName><affiliation>Department of Pathology The University of Chicago Chicago, Illinois 60637, USA</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Stephen C.</forename><surname>Meredith</surname></persName><affiliation>Department of Pathology The University of Chicago Chicago, Illinois 60637, USA</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Christopher</forename><surname>T.Siegel</surname></persName><affiliation>Department of Pathology The University of Chicago Chicago, Illinois 60637, USA</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Hans</forename><surname>Schreiber</surname></persName><affiliation>Department of Pathology The University of Chicago Chicago, Illinois 60637, USA</affiliation></author><idno type="istex">9102E8296D3E74AB60AC334F7E1C2F04827972F9</idno><idno type="ark">ark:/67375/6H6-5HZPSK8J-F</idno><idno type="DOI">10.1016/1074-7613(95)90078-0</idno><idno type="PII">1074-7613(95)90078-0</idno></analytic><monogr><title level="j">Immunity</title><title level="j" type="abbrev">IMMUNI</title><idno type="pISSN">1074-7613</idno><idno type="PII">S1074-7613(00)X0113-6</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995"></date><biblScope unit="volume">2</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="45">45</biblScope><biblScope unit="page" to="59">59</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1995</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Mice immunized against a cancer recognize antigens unique to that cancer, but the molecular structures of such antigens are unknown. We Isolated CD4+ T cell clones recognizing an antigen uniquely expressed on the UV-induced tumor 6132A; some clones inhibited the growth of tumors bearing the specific antigen. A T cell hybridoma was used to purify this antigen from nuclear extracts by RP-HPLC and SDS-PAGE using T cell immunoblot assays. A partial amino acid sequence was nearly identical to a sequence in ribosomal protein L9. The cDNA sequence of L9 from 6132A PRO cells differed from the normal sequence at one nucleotide; this mutation encoded histidine instead of leucine at position 47. A synthetic peptide containing this mutation was over 1000-fold more stimulatory of T cells than was the wild-type peptide. These results indicate that this unique tumor antigen is derived from a single amino acid substitution in a cellular protein.</p></abstract></profileDesc><revisionDesc><change when="1994-11-14">Modified</change><change when="1995">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5HZPSK8J-F/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>IMMUNI</jid><aid>95900780</aid><ce:pii>1074-7613(95)90078-0</ce:pii><ce:doi>10.1016/1074-7613(95)90078-0</ce:doi><ce:copyright type="unknown" year="1995"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Article</ce:textfn></ce:dochead><ce:title>A unique tumor antigen produced by a single amino acid substitution</ce:title><ce:author-group><ce:author><ce:given-name>Paul A.</ce:given-name><ce:surname>Monach</ce:surname></ce:author><ce:author><ce:given-name>Stephen C.</ce:given-name><ce:surname>Meredith</ce:surname></ce:author><ce:author><ce:given-name>Christopher</ce:given-name><ce:surname>T.Siegel</ce:surname></ce:author><ce:author><ce:given-name>Hans</ce:given-name><ce:surname>Schreiber</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Pathology The University of Chicago Chicago, Illinois 60637, USA</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="28" month="10" year="1994"></ce:date-received><ce:date-revised day="14" month="11" year="1994"></ce:date-revised><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Mice immunized against a cancer recognize antigens unique to that cancer, but the molecular structures of such antigens are unknown. We Isolated CD4<ce:sup>+</ce:sup> T cell clones recognizing an antigen uniquely expressed on the UV-induced tumor 6132A; some clones inhibited the growth of tumors bearing the specific antigen. A T cell hybridoma was used to purify this antigen from nuclear extracts by RP-HPLC and SDS-PAGE using T cell immunoblot assays. A partial amino acid sequence was nearly identical to a sequence in ribosomal protein L9. The cDNA sequence of L9 from 6132A PRO cells differed from the normal sequence at one nucleotide; this mutation encoded histidine instead of leucine at position 47. A synthetic peptide containing this mutation was over 1000-fold more stimulatory of T cells than was the wild-type peptide. These results indicate that this unique tumor antigen is derived from a single amino acid substitution in a cellular protein.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>A unique tumor antigen produced by a single amino acid substitution</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>A unique tumor antigen produced by a single amino acid substitution</title></titleInfo><name type="personal"><namePart type="given">Paul A.</namePart><namePart type="family">Monach</namePart><affiliation>Department of Pathology The University of Chicago Chicago, Illinois 60637, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stephen C.</namePart><namePart type="family">Meredith</namePart><affiliation>Department of Pathology The University of Chicago Chicago, Illinois 60637, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Christopher</namePart><namePart type="family">T.Siegel</namePart><affiliation>Department of Pathology The University of Chicago Chicago, Illinois 60637, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hans</namePart><namePart type="family">Schreiber</namePart><affiliation>Department of Pathology The University of Chicago Chicago, Illinois 60637, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1995</dateIssued><dateModified encoding="w3cdtf">1994-11-14</dateModified><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Mice immunized against a cancer recognize antigens unique to that cancer, but the molecular structures of such antigens are unknown. We Isolated CD4+ T cell clones recognizing an antigen uniquely expressed on the UV-induced tumor 6132A; some clones inhibited the growth of tumors bearing the specific antigen. A T cell hybridoma was used to purify this antigen from nuclear extracts by RP-HPLC and SDS-PAGE using T cell immunoblot assays. A partial amino acid sequence was nearly identical to a sequence in ribosomal protein L9. The cDNA sequence of L9 from 6132A PRO cells differed from the normal sequence at one nucleotide; this mutation encoded histidine instead of leucine at position 47. A synthetic peptide containing this mutation was over 1000-fold more stimulatory of T cells than was the wild-type peptide. These results indicate that this unique tumor antigen is derived from a single amino acid substitution in a cellular protein.</abstract><note type="content">Section title: Article</note><relatedItem type="host"><titleInfo><title>Immunity</title></titleInfo><titleInfo type="abbreviated"><title>IMMUNI</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1995</dateIssued></originInfo><identifier type="ISSN">1074-7613</identifier><identifier type="PII">S1074-7613(00)X0113-6</identifier><part><date>1995</date><detail type="volume"><number>2</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>123</end></extent><extent unit="pages"><start>45</start><end>59</end></extent></part></relatedItem><identifier type="istex">9102E8296D3E74AB60AC334F7E1C2F04827972F9</identifier><identifier type="ark">ark:/67375/6H6-5HZPSK8J-F</identifier><identifier type="DOI">10.1016/1074-7613(95)90078-0</identifier><identifier type="PII">1074-7613(95)90078-0</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5HZPSK8J-F/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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