The evaluation of various methods and antigens for the detection of antibodies against Pityrosporum ovale in patients with seborrhoeic dermatitis
Identifieur interne : 001686 ( Istex/Corpus ); précédent : 001685; suivant : 001687The evaluation of various methods and antigens for the detection of antibodies against Pityrosporum ovale in patients with seborrhoeic dermatitis
Auteurs : I. Bergbrant ; S. Johansson ; D. Robbins ; K. Bengtsson ; J. Faergemann ; A. Scheynius ; T. SöderströmSource :
- Clinical and Experimental Dermatology [ 0307-6938 ] ; 1991-09.
Abstract
Sera from 10 patients with seborrhoeic dermatitis and from 10 age‐matched healthy individuals were examined for IgG activity against Pityrosporum ovale. The IgG activity was analysed using the following techniques: an enzyme‐linked immunosorbent assay (ELISA) against whole P. ovale cells, purified cell‐wall carbohydrate or protein extract, an indirect slide‐immunofluorescence assay and fluorescence‐activated flow cytometry using the whole organism as antigen. The ELISA method using the protein antigen was the only technique that showed a significant difference between patients and controls; a lower antibody response was found in the seborrhoeic dermatitis patients compared to healthy controls.
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DOI: 10.1111/j.1365-2230.1991.tb00396.x
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Söderström</name><affiliation><mods:affiliation>Department of Clinical Immunology, University of Gothenburg, Sahlgrenska Hospital, Gothenburg, Sweden</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Clinical and Experimental Dermatology</title><idno type="ISSN">0307-6938</idno><idno type="eISSN">1365-2230</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1991-09">1991-09</date><biblScope unit="volume">16</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="339">339</biblScope><biblScope unit="page" to="343">343</biblScope></imprint><idno type="ISSN">0307-6938</idno></series><idno type="istex">0D1071D70D49F6E29E8810E10A3A8D10701C4EB6</idno><idno type="DOI">10.1111/j.1365-2230.1991.tb00396.x</idno><idno type="ArticleID">CED339</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0307-6938</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sera from 10 patients with seborrhoeic dermatitis and from 10 age‐matched healthy individuals were examined for IgG activity against Pityrosporum ovale. The IgG activity was analysed using the following techniques: an enzyme‐linked immunosorbent assay (ELISA) against whole P. ovale cells, purified cell‐wall carbohydrate or protein extract, an indirect slide‐immunofluorescence assay and fluorescence‐activated flow cytometry using the whole organism as antigen. The ELISA method using the protein antigen was the only technique that showed a significant difference between patients and controls; a lower antibody response was found in the seborrhoeic dermatitis patients compared to healthy controls.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>I.‐M. BERGBRANT</name><affiliations><json:string>Department of Dermatology, University of Gothenburg, Sahlgrenska Hospital, Gothenburg</json:string></affiliations></json:item><json:item><name>S. 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Stockholm</affiliation></author><author><persName><forename type="first">T.</forename><surname>SÖDERSTRÖM</surname></persName><affiliation>Department of Clinical Immunology, University of Gothenburg, Sahlgrenska Hospital, Gothenburg, Sweden</affiliation></author></analytic><monogr><title level="j">Clinical and Experimental Dermatology</title><idno type="pISSN">0307-6938</idno><idno type="eISSN">1365-2230</idno><idno type="DOI">10.1111/(ISSN)1365-2230</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1991-09"></date><biblScope unit="volume">16</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="339">339</biblScope><biblScope unit="page" to="343">343</biblScope></imprint></monogr><idno type="istex">0D1071D70D49F6E29E8810E10A3A8D10701C4EB6</idno><idno type="DOI">10.1111/j.1365-2230.1991.tb00396.x</idno><idno 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The IgG activity was analysed using the following techniques: an enzyme‐linked immunosorbent assay (ELISA) against whole P. ovale cells, purified cell‐wall carbohydrate or protein extract, an indirect slide‐immunofluorescence assay and fluorescence‐activated flow cytometry using the whole organism as antigen. 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1991</unparsedEditorialHistory><countGroup><count type="referenceTotal" number="19"></count><count type="linksCrossRef" number="8"></count></countGroup><titleGroup><title type="main">The evaluation of various methods and antigens for the detection of antibodies against <i>Pityrosporum ovale</i> in patients with seborrhoeic dermatitis</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" corresponding="yes"><personName><givenNames>I.‐M.</givenNames><familyName>BERGBRANT</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2"><personName><givenNames>S.</givenNames><familyName>JOHANSSON</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a3"><personName><givenNames>D.</givenNames><familyName>ROBBINS</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a3"><personName><givenNames>K.</givenNames><familyName>BENGTSSON</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1"><personName><givenNames>J.</givenNames><familyName>FAERGEMANN</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a2"><personName><givenNames>A.</givenNames><familyName>SCHEYNIUS</familyName></personName></creator><creator creatorRole="author" xml:id="cr7" affiliationRef="#a3"><personName><givenNames>T.</givenNames><familyName>SÖDERSTRÖM</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1"><unparsedAffiliation>Department of Dermatology, University of Gothenburg, Sahlgrenska Hospital, Gothenburg</unparsedAffiliation></affiliation><affiliation xml:id="a2"><unparsedAffiliation>Department of‘Clinical Immunology, Karolinska Institute, Karolinska Hospital, Stockholm</unparsedAffiliation></affiliation><affiliation xml:id="a3" countryCode="SE"><unparsedAffiliation>Department of Clinical Immunology, University of Gothenburg, Sahlgrenska Hospital, Gothenburg, Sweden</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p>Sera from 10 patients with seborrhoeic dermatitis and from 10 age‐matched healthy individuals were examined for IgG activity against <i>Pityrosporum ovale.</i> The IgG activity was analysed using the following techniques: an enzyme‐linked immunosorbent assay (ELISA) against whole <i>P. ovale</i> cells, purified cell‐wall carbohydrate or protein extract, an indirect slide‐immunofluorescence assay and fluorescence‐activated flow cytometry using the whole organism as antigen. The ELISA method using the protein antigen was the only technique that showed a significant difference between patients and controls; a lower antibody response was found in the seborrhoeic dermatitis patients compared to healthy controls.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>The evaluation of various methods and antigens for the detection of antibodies against Pityrosporum ovale in patients with seborrhoeic dermatitis</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>The evaluation of various methods and antigens for the detection of antibodies against</title></titleInfo><name type="personal"><namePart type="given">I.‐M.</namePart><namePart type="family">BERGBRANT</namePart><affiliation>Department of Dermatology, University of Gothenburg, Sahlgrenska Hospital, Gothenburg</affiliation><description>Correspondence: Ing‐Marie Berghrant, Department of Dermatology, Sahlgrenska Hospital, S‐413 45 Göteborg, Sweden</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S.</namePart><namePart type="family">JOHANSSON</namePart><affiliation>Department of‘Clinical Immunology, Karolinska Institute, Karolinska Hospital, Stockholm</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">ROBBINS</namePart><affiliation>Department of Clinical Immunology, University of Gothenburg, Sahlgrenska Hospital, Gothenburg, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">BENGTSSON</namePart><affiliation>Department of Clinical Immunology, University of Gothenburg, Sahlgrenska Hospital, Gothenburg, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J.</namePart><namePart type="family">FAERGEMANN</namePart><affiliation>Department of Dermatology, University of Gothenburg, Sahlgrenska Hospital, Gothenburg</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.</namePart><namePart type="family">SCHEYNIUS</namePart><affiliation>Department of‘Clinical Immunology, Karolinska Institute, Karolinska Hospital, Stockholm</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">SÖDERSTRÖM</namePart><affiliation>Department of Clinical Immunology, University of Gothenburg, Sahlgrenska Hospital, Gothenburg, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1991-09</dateIssued><edition>Accepted for publication 28 February 1991</edition><copyrightDate encoding="w3cdtf">1991</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="references">19</extent></physicalDescription><abstract lang="en">Sera from 10 patients with seborrhoeic dermatitis and from 10 age‐matched healthy individuals were examined for IgG activity against Pityrosporum ovale. The IgG activity was analysed using the following techniques: an enzyme‐linked immunosorbent assay (ELISA) against whole P. ovale cells, purified cell‐wall carbohydrate or protein extract, an indirect slide‐immunofluorescence assay and fluorescence‐activated flow cytometry using the whole organism as antigen. The ELISA method using the protein antigen was the only technique that showed a significant difference between patients and controls; a lower antibody response was found in the seborrhoeic dermatitis patients compared to healthy controls.</abstract><relatedItem type="host"><titleInfo><title>Clinical and Experimental Dermatology</title></titleInfo><genre type="Journal">journal</genre><identifier type="ISSN">0307-6938</identifier><identifier type="eISSN">1365-2230</identifier><identifier type="DOI">10.1111/(ISSN)1365-2230</identifier><identifier type="PublisherID">CED</identifier><part><date>1991</date><detail type="volume"><caption>vol.</caption><number>16</number></detail><detail type="issue"><caption>no.</caption><number>5</number></detail><extent unit="pages"><start>339</start><end>343</end><total>5</total></extent></part></relatedItem><identifier type="istex">0D1071D70D49F6E29E8810E10A3A8D10701C4EB6</identifier><identifier type="DOI">10.1111/j.1365-2230.1991.tb00396.x</identifier><identifier type="ArticleID">CED339</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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