Distribution of tritiated dihydromicrocystin in swine
Identifieur interne : 001685 ( Istex/Corpus ); précédent : 001684; suivant : 001686Distribution of tritiated dihydromicrocystin in swine
Auteurs : Richard R. Stotts ; A. Robert Twardock ; Wanda M. Haschek ; Byoung W. Choi ; Kenneth L. Rinehart ; Val R. BeasleySource :
- Toxicon [ 0041-0101 ] ; 1996.
Abstract
The distribution of tritiated dihydromicrocystin i [3H]2H-MCLR was studied in anesthetized specific-pathogen-free pig. Two doses were administered i.m. and one dose was given via an isolated ileal loop. At 4 hr after i.v. administration of the toxin at 25 μg/kg, 64.6% of the total dose (% TD) was located in the liver, with small amounts distributed to the kidneys (1.2% TD), lungs (1.75% TD), heart (0.22% TD), ileum (0.13% TD) and spleen (0.04% TD). A similar distribution was found at 4 hr postdosing in pigs given 75 μg/kg, although the liver contained a lower fraction of the total dose, at 46.99% TD, and the kidneys had somewhat more, at 2.19% TD, than the low dose. At the high dose, the fractions of the amount given accounted for by the lungs (0.55% TD), heart (0.23% TD), ileum (0.20% TD) and spleen (0.07% TD) were similar to those at the low dose. The livers of the pigs given 75 μg/kg via the ileal loop, at 5 hr postdosing, contained 49.5% TD and the ileum had 33.94% TD. Smaller amounts were distributed to kidneys (1.04% TD), lungs (0.65% TD), heart (0.81% TD) and spleen (0.16% TD). The livers of both groups dose at 75 μg/kg contained higher concentrations of toxin, but lower percentages of the total dose, than the livers of pigs dosed at 25 μg/kg. Larger increases in serum arginase in the two 75 μg/kg groups were associated with histological evidence of more severe liver damage than at the 25 μg/kg dose. Analysis of radiolabelled compounds from hepatic tissue using fast atom bombardment mass spectrometry determined that the primary constituent was [3H]2H-MCLR, but two minor radioactive components were also isolated. These findings indicate that [3H]2H-MCLR is rapidly concentrated in the liver of swine, whether given i.v. or via an isolated ileal loop, that at extremely toxic doses uptake is slowed, and that it is as toxicologically active as the parent compound.
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DOI: 10.1016/S0041-0101(96)00169-9
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Distribution of tritiated dihydromicrocystin in swine</title><author><name sortKey="Stotts, Richard R" sort="Stotts, Richard R" uniqKey="Stotts R" first="Richard R." last="Stotts">Richard R. Stotts</name><affiliation><mods:affiliation>Department of Veterinary Biosciences, University of Illinois, Urbana, IL 610801, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Twardock, A Robert" sort="Twardock, A Robert" uniqKey="Twardock A" first="A. Robert" last="Twardock">A. Robert Twardock</name><affiliation><mods:affiliation>Department of Veterinary Biosciences, University of Illinois, Urbana, IL 610801, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Haschek, Wanda M" sort="Haschek, Wanda M" uniqKey="Haschek W" first="Wanda M." last="Haschek">Wanda M. Haschek</name><affiliation><mods:affiliation>Department of Veterinary Pathobiology, University of Illinois, Urbana, IL 610801, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Choi, Byoung W" sort="Choi, Byoung W" uniqKey="Choi B" first="Byoung W." last="Choi">Byoung W. Choi</name><affiliation><mods:affiliation>Department of Chemistry, University of Illinois, Urbana, IL 610801, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Rinehart, Kenneth L" sort="Rinehart, Kenneth L" uniqKey="Rinehart K" first="Kenneth L." last="Rinehart">Kenneth L. Rinehart</name><affiliation><mods:affiliation>Department of Chemistry, University of Illinois, Urbana, IL 610801, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Beasley, Val R" sort="Beasley, Val R" uniqKey="Beasley V" first="Val R." last="Beasley">Val R. Beasley</name><affiliation><mods:affiliation>Author to whom correspondence should be addressed.</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Veterinary Biosciences, University of Illinois, Urbana, IL 610801, U.S.A.</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:B3CC0187208543A58510F1BB708B5ECF516510AF</idno><date when="1997" year="1997">1997</date><idno type="doi">10.1016/S0041-0101(96)00169-9</idno><idno type="url">https://api.istex.fr/document/B3CC0187208543A58510F1BB708B5ECF516510AF/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001685</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Distribution of tritiated dihydromicrocystin in swine</title><author><name sortKey="Stotts, Richard R" sort="Stotts, Richard R" uniqKey="Stotts R" first="Richard R." last="Stotts">Richard R. Stotts</name><affiliation><mods:affiliation>Department of Veterinary Biosciences, University of Illinois, Urbana, IL 610801, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Twardock, A Robert" sort="Twardock, A Robert" uniqKey="Twardock A" first="A. Robert" last="Twardock">A. Robert Twardock</name><affiliation><mods:affiliation>Department of Veterinary Biosciences, University of Illinois, Urbana, IL 610801, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Haschek, Wanda M" sort="Haschek, Wanda M" uniqKey="Haschek W" first="Wanda M." last="Haschek">Wanda M. Haschek</name><affiliation><mods:affiliation>Department of Veterinary Pathobiology, University of Illinois, Urbana, IL 610801, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Choi, Byoung W" sort="Choi, Byoung W" uniqKey="Choi B" first="Byoung W." last="Choi">Byoung W. Choi</name><affiliation><mods:affiliation>Department of Chemistry, University of Illinois, Urbana, IL 610801, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Rinehart, Kenneth L" sort="Rinehart, Kenneth L" uniqKey="Rinehart K" first="Kenneth L." last="Rinehart">Kenneth L. Rinehart</name><affiliation><mods:affiliation>Department of Chemistry, University of Illinois, Urbana, IL 610801, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Beasley, Val R" sort="Beasley, Val R" uniqKey="Beasley V" first="Val R." last="Beasley">Val R. Beasley</name><affiliation><mods:affiliation>Author to whom correspondence should be addressed.</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Veterinary Biosciences, University of Illinois, Urbana, IL 610801, U.S.A.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Toxicon</title><title level="j" type="abbrev">TOXCON</title><idno type="ISSN">0041-0101</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">35</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="937">937</biblScope><biblScope unit="page" to="953">953</biblScope></imprint><idno type="ISSN">0041-0101</idno></series><idno type="istex">B3CC0187208543A58510F1BB708B5ECF516510AF</idno><idno type="DOI">10.1016/S0041-0101(96)00169-9</idno><idno type="PII">S0041-0101(96)00169-9</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0041-0101</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The distribution of tritiated dihydromicrocystin i [3H]2H-MCLR was studied in anesthetized specific-pathogen-free pig. Two doses were administered i.m. and one dose was given via an isolated ileal loop. At 4 hr after i.v. administration of the toxin at 25 μg/kg, 64.6% of the total dose (% TD) was located in the liver, with small amounts distributed to the kidneys (1.2% TD), lungs (1.75% TD), heart (0.22% TD), ileum (0.13% TD) and spleen (0.04% TD). A similar distribution was found at 4 hr postdosing in pigs given 75 μg/kg, although the liver contained a lower fraction of the total dose, at 46.99% TD, and the kidneys had somewhat more, at 2.19% TD, than the low dose. At the high dose, the fractions of the amount given accounted for by the lungs (0.55% TD), heart (0.23% TD), ileum (0.20% TD) and spleen (0.07% TD) were similar to those at the low dose. The livers of the pigs given 75 μg/kg via the ileal loop, at 5 hr postdosing, contained 49.5% TD and the ileum had 33.94% TD. Smaller amounts were distributed to kidneys (1.04% TD), lungs (0.65% TD), heart (0.81% TD) and spleen (0.16% TD). The livers of both groups dose at 75 μg/kg contained higher concentrations of toxin, but lower percentages of the total dose, than the livers of pigs dosed at 25 μg/kg. Larger increases in serum arginase in the two 75 μg/kg groups were associated with histological evidence of more severe liver damage than at the 25 μg/kg dose. Analysis of radiolabelled compounds from hepatic tissue using fast atom bombardment mass spectrometry determined that the primary constituent was [3H]2H-MCLR, but two minor radioactive components were also isolated. These findings indicate that [3H]2H-MCLR is rapidly concentrated in the liver of swine, whether given i.v. or via an isolated ileal loop, that at extremely toxic doses uptake is slowed, and that it is as toxicologically active as the parent compound.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Richard R. Stotts</name><affiliations><json:string>Department of Veterinary Biosciences, University of Illinois, Urbana, IL 610801, U.S.A.</json:string></affiliations></json:item><json:item><name>A.Robert Twardock</name><affiliations><json:string>Department of Veterinary Biosciences, University of Illinois, Urbana, IL 610801, U.S.A.</json:string></affiliations></json:item><json:item><name>Wanda M. Haschek</name><affiliations><json:string>Department of Veterinary Pathobiology, University of Illinois, Urbana, IL 610801, U.S.A.</json:string></affiliations></json:item><json:item><name>Byoung W. Choi</name><affiliations><json:string>Department of Chemistry, University of Illinois, Urbana, IL 610801, U.S.A.</json:string></affiliations></json:item><json:item><name>Kenneth L. Rinehart</name><affiliations><json:string>Department of Chemistry, University of Illinois, Urbana, IL 610801, U.S.A.</json:string></affiliations></json:item><json:item><name>Val R. Beasley</name><affiliations><json:string>Author to whom correspondence should be addressed.</json:string><json:string>Department of Veterinary Biosciences, University of Illinois, Urbana, IL 610801, U.S.A.</json:string></affiliations></json:item></author><language><json:string>eng</json:string></language><abstract>The distribution of tritiated dihydromicrocystin i [3H]2H-MCLR was studied in anesthetized specific-pathogen-free pig. Two doses were administered i.m. and one dose was given via an isolated ileal loop. At 4 hr after i.v. administration of the toxin at 25 μg/kg, 64.6% of the total dose (% TD) was located in the liver, with small amounts distributed to the kidneys (1.2% TD), lungs (1.75% TD), heart (0.22% TD), ileum (0.13% TD) and spleen (0.04% TD). A similar distribution was found at 4 hr postdosing in pigs given 75 μg/kg, although the liver contained a lower fraction of the total dose, at 46.99% TD, and the kidneys had somewhat more, at 2.19% TD, than the low dose. At the high dose, the fractions of the amount given accounted for by the lungs (0.55% TD), heart (0.23% TD), ileum (0.20% TD) and spleen (0.07% TD) were similar to those at the low dose. The livers of the pigs given 75 μg/kg via the ileal loop, at 5 hr postdosing, contained 49.5% TD and the ileum had 33.94% TD. Smaller amounts were distributed to kidneys (1.04% TD), lungs (0.65% TD), heart (0.81% TD) and spleen (0.16% TD). The livers of both groups dose at 75 μg/kg contained higher concentrations of toxin, but lower percentages of the total dose, than the livers of pigs dosed at 25 μg/kg. Larger increases in serum arginase in the two 75 μg/kg groups were associated with histological evidence of more severe liver damage than at the 25 μg/kg dose. Analysis of radiolabelled compounds from hepatic tissue using fast atom bombardment mass spectrometry determined that the primary constituent was [3H]2H-MCLR, but two minor radioactive components were also isolated. These findings indicate that [3H]2H-MCLR is rapidly concentrated in the liver of swine, whether given i.v. or via an isolated ileal loop, that at extremely toxic doses uptake is slowed, and that it is as toxicologically active as the parent compound.</abstract><qualityIndicators><score>7.73</score><pdfVersion>1.3</pdfVersion><pdfPageSize>497 x 698 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>1897</abstractCharCount><pdfWordCount>4730</pdfWordCount><pdfCharCount>32384</pdfCharCount><pdfPageCount>17</pdfPageCount><abstractWordCount>319</abstractWordCount></qualityIndicators><title>Distribution of tritiated dihydromicrocystin in swine</title><pii><json:string>S0041-0101(96)00169-9</json:string></pii><genre><json:string>research-article</json:string></genre><serie><volume>86</volume><pages><last>774</last><first>770</first></pages><genre></genre><language><json:string>unknown</json:string></language><title>Proc. natl. Acad. Sci. 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Two doses were administered i.m. and one dose was given via an isolated ileal loop. At 4 hr after i.v. administration of the toxin at 25 μg/kg, 64.6% of the total dose (% TD) was located in the liver, with small amounts distributed to the kidneys (1.2% TD), lungs (1.75% TD), heart (0.22% TD), ileum (0.13% TD) and spleen (0.04% TD). A similar distribution was found at 4 hr postdosing in pigs given 75 μg/kg, although the liver contained a lower fraction of the total dose, at 46.99% TD, and the kidneys had somewhat more, at 2.19% TD, than the low dose. At the high dose, the fractions of the amount given accounted for by the lungs (0.55% TD), heart (0.23% TD), ileum (0.20% TD) and spleen (0.07% TD) were similar to those at the low dose. The livers of the pigs given 75 μg/kg via the ileal loop, at 5 hr postdosing, contained 49.5% TD and the ileum had 33.94% TD. Smaller amounts were distributed to kidneys (1.04% TD), lungs (0.65% TD), heart (0.81% TD) and spleen (0.16% TD). The livers of both groups dose at 75 μg/kg contained higher concentrations of toxin, but lower percentages of the total dose, than the livers of pigs dosed at 25 μg/kg. Larger increases in serum arginase in the two 75 μg/kg groups were associated with histological evidence of more severe liver damage than at the 25 μg/kg dose. Analysis of radiolabelled compounds from hepatic tissue using fast atom bombardment mass spectrometry determined that the primary constituent was [3H]2H-MCLR, but two minor radioactive components were also isolated. These findings indicate that [3H]2H-MCLR is rapidly concentrated in the liver of swine, whether given i.v. or via an isolated ileal loop, that at extremely toxic doses uptake is slowed, and that it is as toxicologically active as the parent compound.</p></abstract></profileDesc><revisionDesc><change when="1996-08-16">Registration</change><change when="1996">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>TOXCON</jid><aid>96001699</aid><ce:pii>S0041-0101(96)00169-9</ce:pii><ce:doi>10.1016/S0041-0101(96)00169-9</ce:doi><ce:copyright type="unknown" year="1997"></ce:copyright></item-info><head><ce:title>Distribution of tritiated dihydromicrocystin in swine</ce:title><ce:author-group><ce:author><ce:given-name>Richard R.</ce:given-name><ce:surname>Stotts</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>A.Robert</ce:given-name><ce:surname>Twardock</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Wanda M.</ce:given-name><ce:surname>Haschek</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Byoung W.</ce:given-name><ce:surname>Choi</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Kenneth L.</ce:given-name><ce:surname>Rinehart</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Val R.</ce:given-name><ce:surname>Beasley</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>1</ce:label><ce:textfn>Department of Veterinary Biosciences, University of Illinois, Urbana, IL 610801, U.S.A.</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>2</ce:label><ce:textfn>Department of Veterinary Pathobiology, University of Illinois, Urbana, IL 610801, U.S.A.</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>3</ce:label><ce:textfn>Department of Chemistry, University of Illinois, Urbana, IL 610801, U.S.A.</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Author to whom correspondence should be addressed.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="21" month="3" year="1996"></ce:date-received><ce:date-accepted day="16" month="8" year="1996"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The distribution of tritiated dihydromicrocystin i [<ce:sup loc="pre">3</ce:sup>H]2H-MCLR was studied in anesthetized specific-pathogen-free pig. Two doses were administered i.m. and one dose was given via an isolated ileal loop. At 4 hr after i.v. administration of the toxin at 25 μg/kg, 64.6% of the total dose (% TD) was located in the liver, with small amounts distributed to the kidneys (1.2% TD), lungs (1.75% TD), heart (0.22% TD), ileum (0.13% TD) and spleen (0.04% TD). A similar distribution was found at 4 hr postdosing in pigs given 75 μg/kg, although the liver contained a lower fraction of the total dose, at 46.99% TD, and the kidneys had somewhat more, at 2.19% TD, than the low dose. At the high dose, the fractions of the amount given accounted for by the lungs (0.55% TD), heart (0.23% TD), ileum (0.20% TD) and spleen (0.07% TD) were similar to those at the low dose. The livers of the pigs given 75 μg/kg via the ileal loop, at 5 hr postdosing, contained 49.5% TD and the ileum had 33.94% TD. Smaller amounts were distributed to kidneys (1.04% TD), lungs (0.65% TD), heart (0.81% TD) and spleen (0.16% TD). The livers of both groups dose at 75 μg/kg contained higher concentrations of toxin, but lower percentages of the total dose, than the livers of pigs dosed at 25 μg/kg. Larger increases in serum arginase in the two 75 μg/kg groups were associated with histological evidence of more severe liver damage than at the 25 μg/kg dose. Analysis of radiolabelled compounds from hepatic tissue using fast atom bombardment mass spectrometry determined that the primary constituent was [<ce:sup loc="pre">3</ce:sup>H]2H-MCLR, but two minor radioactive components were also isolated. These findings indicate that [<ce:sup loc="pre">3</ce:sup>H]2H-MCLR is rapidly concentrated in the liver of swine, whether given i.v. or via an isolated ileal loop, that at extremely toxic doses uptake is slowed, and that it is as toxicologically active as the parent compound.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Distribution of tritiated dihydromicrocystin in swine</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Distribution of tritiated dihydromicrocystin in swine</title></titleInfo><name type="personal"><namePart type="given">Richard R.</namePart><namePart type="family">Stotts</namePart><affiliation>Department of Veterinary Biosciences, University of Illinois, Urbana, IL 610801, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A.Robert</namePart><namePart type="family">Twardock</namePart><affiliation>Department of Veterinary Biosciences, University of Illinois, Urbana, IL 610801, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wanda M.</namePart><namePart type="family">Haschek</namePart><affiliation>Department of Veterinary Pathobiology, University of Illinois, Urbana, IL 610801, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Byoung W.</namePart><namePart type="family">Choi</namePart><affiliation>Department of Chemistry, University of Illinois, Urbana, IL 610801, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kenneth L.</namePart><namePart type="family">Rinehart</namePart><affiliation>Department of Chemistry, University of Illinois, Urbana, IL 610801, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Val R.</namePart><namePart type="family">Beasley</namePart><affiliation>Author to whom correspondence should be addressed.</affiliation><affiliation>Department of Veterinary Biosciences, University of Illinois, Urbana, IL 610801, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1996</dateIssued><dateValid encoding="w3cdtf">1996-08-16</dateValid><copyrightDate encoding="w3cdtf">1997</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The distribution of tritiated dihydromicrocystin i [3H]2H-MCLR was studied in anesthetized specific-pathogen-free pig. Two doses were administered i.m. and one dose was given via an isolated ileal loop. At 4 hr after i.v. administration of the toxin at 25 μg/kg, 64.6% of the total dose (% TD) was located in the liver, with small amounts distributed to the kidneys (1.2% TD), lungs (1.75% TD), heart (0.22% TD), ileum (0.13% TD) and spleen (0.04% TD). A similar distribution was found at 4 hr postdosing in pigs given 75 μg/kg, although the liver contained a lower fraction of the total dose, at 46.99% TD, and the kidneys had somewhat more, at 2.19% TD, than the low dose. At the high dose, the fractions of the amount given accounted for by the lungs (0.55% TD), heart (0.23% TD), ileum (0.20% TD) and spleen (0.07% TD) were similar to those at the low dose. The livers of the pigs given 75 μg/kg via the ileal loop, at 5 hr postdosing, contained 49.5% TD and the ileum had 33.94% TD. Smaller amounts were distributed to kidneys (1.04% TD), lungs (0.65% TD), heart (0.81% TD) and spleen (0.16% TD). The livers of both groups dose at 75 μg/kg contained higher concentrations of toxin, but lower percentages of the total dose, than the livers of pigs dosed at 25 μg/kg. Larger increases in serum arginase in the two 75 μg/kg groups were associated with histological evidence of more severe liver damage than at the 25 μg/kg dose. Analysis of radiolabelled compounds from hepatic tissue using fast atom bombardment mass spectrometry determined that the primary constituent was [3H]2H-MCLR, but two minor radioactive components were also isolated. These findings indicate that [3H]2H-MCLR is rapidly concentrated in the liver of swine, whether given i.v. or via an isolated ileal loop, that at extremely toxic doses uptake is slowed, and that it is as toxicologically active as the parent compound.</abstract><relatedItem type="host"><titleInfo><title>Toxicon</title></titleInfo><titleInfo type="abbreviated"><title>TOXCON</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199706</dateIssued></originInfo><identifier type="ISSN">0041-0101</identifier><identifier type="PII">S0041-0101(00)X0034-7</identifier><part><date>199706</date><detail type="volume"><number>35</number><caption>vol.</caption></detail><detail type="issue"><number>6</number><caption>no.</caption></detail><extent unit="issue pages"><start>801</start><end>995</end></extent><extent unit="pages"><start>937</start><end>953</end></extent></part></relatedItem><identifier type="istex">B3CC0187208543A58510F1BB708B5ECF516510AF</identifier><identifier type="DOI">10.1016/S0041-0101(96)00169-9</identifier><identifier type="PII">S0041-0101(96)00169-9</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/B3CC0187208543A58510F1BB708B5ECF516510AF/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">PHARMACOLOGY & PHARMACY</classCode><classCode scheme="WOS">TOXICOLOGY</classCode><classCode scheme="WOS">TRANSPLANTATION</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments></istex></record>Pour manipuler ce document sous Unix (Dilib)
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