LrgpV1, PascalFrancis, Checkpoint, bibRecord, 000395

Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate

Identifieur interne : 000395 ( PascalFrancis/Checkpoint ); précédent : 000394; suivant : 000396

Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate

Auteurs : Kaspar Koch [Pays-Bas] ; Rutger J. F. Van Den Berg [Pays-Bas] ; Pieter J. Nieuwland [Pays-Bas] ; Roel Wijtmans [Pays-Bas] ; Marcel G. Wubbolts [Pays-Bas] ; Hans E. Schoemaker [Pays-Bas] ; Floris P. J. T. Rutjes [Pays-Bas] ; Jan C. M. Van Hest [Pays-Bas]

Source :

Chemical engineering journal : (1996) [ 1385-8947 ] ; 2008.

RBID : Pascal:08-0055156

Descripteurs français

Pascal (Inist)
- Synthèse enzymatique, Microréacteur, Système biphasique, Biocatalyseur, En discontinu, Réaction enzymatique, Vitesse réaction, Cinétique, Enantiosélectivité, Agitation mécanique.

English descriptors

KwdEn :
- Batchwise, Biocatalyst, Biphasic system, Enantioselectivity, Enzymatic reaction, Enzymatic synthesis, Kinetics, Microreactor, Reaction rate, Stirring.

Abstract

The synthesis of optically pure cyanohydrins using a crude cell lysate containing the enzyme hydroxynitrile lyase (HNL) was studied in a microreactor in an aqueous-organic biphasic system. Different aldehyde substrates were selected to be converted to their corresponding cyanohydrins. It was successfully demonstrated that this crude cell lysate could readily be applied as a biocatalyst in a microchannel without clogging the channels. The biocatalytic activity toward the different substrates could rapidly be investigated with only small quantities of enzyme needed, compared to batch scale screening. Furthermore, the optimal contact between two immiscible phases in a microreactor resulted in enzymatic reactions with a high initial reaction rate and enantioselectivity, comparable to a batchwise process in which optimized conditions were achieved by vigorous stirring. Thus, performing the selected enzymatic reaction in a microreactor is a facile and cost efficient screening method leading to results which can be directly translated to batchwise processes.

Affiliations:

Pays-Bas

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Le document en format XML

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<front><div type="abstract" xml:lang="en">The synthesis of optically pure cyanohydrins using a crude cell lysate containing the enzyme hydroxynitrile lyase (HNL) was studied in a microreactor in an aqueous-organic biphasic system. Different aldehyde substrates were selected to be converted to their corresponding cyanohydrins. It was successfully demonstrated that this crude cell lysate could readily be applied as a biocatalyst in a microchannel without clogging the channels. The biocatalytic activity toward the different substrates could rapidly be investigated with only small quantities of enzyme needed, compared to batch scale screening. Furthermore, the optimal contact between two immiscible phases in a microreactor resulted in enzymatic reactions with a high initial reaction rate and enantioselectivity, comparable to a batchwise process in which optimized conditions were achieved by vigorous stirring. Thus, performing the selected enzymatic reaction in a microreactor is a facile and cost efficient screening method leading to results which can be directly translated to batchwise processes.</div>
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<fC03 i1="02" i2="X" l="ENG"><s0>Microreactor</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Microreactor</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Système biphasique</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Biphasic system</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Sistema bifásico</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Biocatalyseur</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Biocatalyst</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Biocatalizador</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>En discontinu</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Batchwise</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>En discontinuo</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Réaction enzymatique</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Enzymatic reaction</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Reacción enzimática</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Vitesse réaction</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Reaction rate</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Velocidad reacción</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Cinétique</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Kinetics</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Cinética</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Enantiosélectivité</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Enantioselectivity</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Enantioselectividad</s0>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Agitation mécanique</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Stirring</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Agitación mecánica</s0>
<s5>10</s5>
</fC03>
<fN21><s1>028</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
<pR><fA30 i1="01" i2="1" l="ENG"><s1>IMRET 9 : International Conference on Microreaction Technology</s1>
<s2>9</s2>
<s3>Potsdam DEU</s3>
<s4>2006-09-06</s4>
</fA30>
</pR>
</standard>
</inist>
<affiliations><list><country><li>Pays-Bas</li>
</country>
</list>
<tree><country name="Pays-Bas"><noRegion><name sortKey="Koch, Kaspar" sort="Koch, Kaspar" uniqKey="Koch K" first="Kaspar" last="Koch">Kaspar Koch</name>
</noRegion>
<name sortKey="Nieuwland, Pieter J" sort="Nieuwland, Pieter J" uniqKey="Nieuwland P" first="Pieter J." last="Nieuwland">Pieter J. Nieuwland</name>
<name sortKey="Rutjes, Floris P J T" sort="Rutjes, Floris P J T" uniqKey="Rutjes F" first="Floris P. J. T." last="Rutjes">Floris P. J. T. Rutjes</name>
<name sortKey="Schoemaker, Hans E" sort="Schoemaker, Hans E" uniqKey="Schoemaker H" first="Hans E." last="Schoemaker">Hans E. Schoemaker</name>
<name sortKey="Van Den Berg, Rutger J F" sort="Van Den Berg, Rutger J F" uniqKey="Van Den Berg R" first="Rutger J. F." last="Van Den Berg">Rutger J. F. Van Den Berg</name>
<name sortKey="Van Hest, Jan C M" sort="Van Hest, Jan C M" uniqKey="Van Hest J" first="Jan C. M." last="Van Hest">Jan C. M. Van Hest</name>
<name sortKey="Wijtmans, Roel" sort="Wijtmans, Roel" uniqKey="Wijtmans R" first="Roel" last="Wijtmans">Roel Wijtmans</name>
<name sortKey="Wubbolts, Marcel G" sort="Wubbolts, Marcel G" uniqKey="Wubbolts M" first="Marcel G." last="Wubbolts">Marcel G. Wubbolts</name>
</country>
</tree>
</affiliations>
</record>

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Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate

Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate

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