LrgpV1, PascalFrancis, Corpus, bibRecord, 000433

Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate

Identifieur interne : 000433 ( PascalFrancis/Corpus ); précédent : 000432; suivant : 000434

Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate

Auteurs : Kaspar Koch ; Rutger J. F. Van Den Berg ; Pieter J. Nieuwland ; Roel Wijtmans ; Marcel G. Wubbolts ; Hans E. Schoemaker ; Floris P. J. T. Rutjes ; Jan C. M. Van Hest

Source :

Chemical engineering journal : (1996) [ 1385-8947 ] ; 2008.

RBID : Pascal:08-0055156

Descripteurs français

Pascal (Inist)
- Synthèse enzymatique, Microréacteur, Système biphasique, Biocatalyseur, En discontinu, Réaction enzymatique, Vitesse réaction, Cinétique, Enantiosélectivité, Agitation mécanique.

English descriptors

KwdEn :
- Batchwise, Biocatalyst, Biphasic system, Enantioselectivity, Enzymatic reaction, Enzymatic synthesis, Kinetics, Microreactor, Reaction rate, Stirring.

Abstract

The synthesis of optically pure cyanohydrins using a crude cell lysate containing the enzyme hydroxynitrile lyase (HNL) was studied in a microreactor in an aqueous-organic biphasic system. Different aldehyde substrates were selected to be converted to their corresponding cyanohydrins. It was successfully demonstrated that this crude cell lysate could readily be applied as a biocatalyst in a microchannel without clogging the channels. The biocatalytic activity toward the different substrates could rapidly be investigated with only small quantities of enzyme needed, compared to batch scale screening. Furthermore, the optimal contact between two immiscible phases in a microreactor resulted in enzymatic reactions with a high initial reaction rate and enantioselectivity, comparable to a batchwise process in which optimized conditions were achieved by vigorous stirring. Thus, performing the selected enzymatic reaction in a microreactor is a facile and cost efficient screening method leading to results which can be directly translated to batchwise processes.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A03		`1`		`@0 Chem. eng. j. : (1996)`
A05				`@2 135`
A06				`@3 SUP1`
A08	`01`	`1`	`ENG`	`@1 Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate`
A09	`01`	`1`	`ENG`	`@1 Microreaction Technology, IMRET 9: Proceedings of the Ninth International Conference on Microreaction Technology`
A11	`01`	`1`		`@1 KOCH (Kaspar)`
A11	`02`	`1`		`@1 VAN DEN BERG (Rutger J. F.)`
A11	`03`	`1`		`@1 NIEUWLAND (Pieter J.)`
A11	`04`	`1`		`@1 WIJTMANS (Roel)`
A11	`05`	`1`		`@1 WUBBOLTS (Marcel G.)`
A11	`06`	`1`		`@1 SCHOEMAKER (Hans E.)`
A11	`07`	`1`		`@1 RUTJES (Floris P. J. T.)`
A11	`08`	`1`		`@1 VAN HEST (Jan C. M.)`
A12	`01`	`1`		`@1 SCHÜTTE (Rüdiger) @9 ed.`
A12	`02`	`1`		`@1 RENKEN (Albert) @9 ed.`
A12	`03`	`1`		`@1 KLEMM (Elias) @9 ed.`
A12	`04`	`1`		`@1 LIAUW (Marcel A.) @9 ed.`
A12	`05`	`1`		`@1 MATLOSZ (Michael) @9 ed.`
A14	`01`			`@1 Institute for Molecules and Materials, Radboud University Nijmegen, Toemooiveld 1 @2 6525 ED Nijmegen @3 NLD @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 7 aut. @Z 8 aut.`
A14	`02`			`@1 DSM Research, PO Box 18 @2 6160 MD Geleen @3 NLD @Z 5 aut. @Z 6 aut.`
A15	`01`			`@1 Process Technology & Engineering, Evonik Degussa GmbH, Rodenbacher Chaussee 4 @2 63457 Hanau (Wolfgang) @3 DEU @Z 1 aut.`
A15	`02`			`@1 Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), SB-ISIC-LGRC-Station 6, Bâtiment CH / CH J2 500 @2 1015 Lausanne @3 CHE @Z 2 aut.`
A15	`03`			`@1 Chemnitz University of Technology, Faculty of Natural Sciences, Department of Chemical Technology @2 09107 Chemnitz @3 DEU @Z 3 aut.`
A15	`04`			`@1 Technical Chemistry and Reaction Engineering, ITMC, RWTH Aachen, Worringerweg 1 @2 52074 Aachen @3 DEU @Z 4 aut.`
A15	`05`			`@1 CNRS-ENSIC, Laboratoire des Sciences du Genie Chimique, 1, rue Grandville @2 54001 Nancy @3 FRA @Z 5 aut.`
A20				`@2 S89-S92`
A21				`@1 2008`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 14678 @5 354000173568260120`
A44				`@0 0000 @1 © 2008 INIST-CNRS. All rights reserved.`
A45				`@0 12 ref.`
A47	`01`	`1`		`@0 08-0055156`
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A64	`01`	`1`		`@0 Chemical engineering journal : (1996)`
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C01	`01`		`ENG`	@0 The synthesis of optically pure cyanohydrins using a crude cell lysate containing the enzyme hydroxynitrile lyase (HNL) was studied in a microreactor in an aqueous-organic biphasic system. Different aldehyde substrates were selected to be converted to their corresponding cyanohydrins. It was successfully demonstrated that this crude cell lysate could readily be applied as a biocatalyst in a microchannel without clogging the channels. The biocatalytic activity toward the different substrates could rapidly be investigated with only small quantities of enzyme needed, compared to batch scale screening. Furthermore, the optimal contact between two immiscible phases in a microreactor resulted in enzymatic reactions with a high initial reaction rate and enantioselectivity, comparable to a batchwise process in which optimized conditions were achieved by vigorous stirring. Thus, performing the selected enzymatic reaction in a microreactor is a facile and cost efficient screening method leading to results which can be directly translated to batchwise processes.
C02	`01`	`X`		`@0 001D07H`
C02	`02`	`X`		`@0 002A31C09C`
C02	`03`	`X`		`@0 215`
C03	`01`	`X`	`FRE`	`@0 Synthèse enzymatique @5 01`
C03	`01`	`X`	`ENG`	`@0 Enzymatic synthesis @5 01`
C03	`01`	`X`	`SPA`	`@0 Síntesis enzimática @5 01`
C03	`02`	`X`	`FRE`	`@0 Microréacteur @5 02`
C03	`02`	`X`	`ENG`	`@0 Microreactor @5 02`
C03	`02`	`X`	`SPA`	`@0 Microreactor @5 02`
C03	`03`	`X`	`FRE`	`@0 Système biphasique @5 03`
C03	`03`	`X`	`ENG`	`@0 Biphasic system @5 03`
C03	`03`	`X`	`SPA`	`@0 Sistema bifásico @5 03`
C03	`04`	`X`	`FRE`	`@0 Biocatalyseur @5 04`
C03	`04`	`X`	`ENG`	`@0 Biocatalyst @5 04`
C03	`04`	`X`	`SPA`	`@0 Biocatalizador @5 04`
C03	`05`	`X`	`FRE`	`@0 En discontinu @5 05`
C03	`05`	`X`	`ENG`	`@0 Batchwise @5 05`
C03	`05`	`X`	`SPA`	`@0 En discontinuo @5 05`
C03	`06`	`X`	`FRE`	`@0 Réaction enzymatique @5 06`
C03	`06`	`X`	`ENG`	`@0 Enzymatic reaction @5 06`
C03	`06`	`X`	`SPA`	`@0 Reacción enzimática @5 06`
C03	`07`	`X`	`FRE`	`@0 Vitesse réaction @5 07`
C03	`07`	`X`	`ENG`	`@0 Reaction rate @5 07`
C03	`07`	`X`	`SPA`	`@0 Velocidad reacción @5 07`
C03	`08`	`X`	`FRE`	`@0 Cinétique @5 08`
C03	`08`	`X`	`ENG`	`@0 Kinetics @5 08`
C03	`08`	`X`	`SPA`	`@0 Cinética @5 08`
C03	`09`	`X`	`FRE`	`@0 Enantiosélectivité @5 09`
C03	`09`	`X`	`ENG`	`@0 Enantioselectivity @5 09`
C03	`09`	`X`	`SPA`	`@0 Enantioselectividad @5 09`
C03	`10`	`X`	`FRE`	`@0 Agitation mécanique @5 10`
C03	`10`	`X`	`ENG`	`@0 Stirring @5 10`
C03	`10`	`X`	`SPA`	`@0 Agitación mecánica @5 10`
N21				`@1 028`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

A30	`01`	`1`	`ENG`	`@1 IMRET 9 : International Conference on Microreaction Technology @2 9 @3 Potsdam DEU @4 2006-09-06`

Format Inist (serveur)

NO :	PASCAL 08-0055156 INIST
ET :	Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate
AU :	KOCH (Kaspar); VAN DEN BERG (Rutger J. F.); NIEUWLAND (Pieter J.); WIJTMANS (Roel); WUBBOLTS (Marcel G.); SCHOEMAKER (Hans E.); RUTJES (Floris P. J. T.); VAN HEST (Jan C. M.); SCHÜTTE (Rüdiger); RENKEN (Albert); KLEMM (Elias); LIAUW (Marcel A.); MATLOSZ (Michael)
AF :	Institute for Molecules and Materials, Radboud University Nijmegen, Toemooiveld 1/6525 ED Nijmegen/Pays-Bas (1 aut., 2 aut., 3 aut., 4 aut., 7 aut., 8 aut.); DSM Research, PO Box 18/6160 MD Geleen/Pays-Bas (5 aut., 6 aut.); Process Technology & Engineering, Evonik Degussa GmbH, Rodenbacher Chaussee 4/63457 Hanau (Wolfgang)/Allemagne (1 aut.); Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), SB-ISIC-LGRC-Station 6, Bâtiment CH / CH J2 500/1015 Lausanne/Suisse (2 aut.); Chemnitz University of Technology, Faculty of Natural Sciences, Department of Chemical Technology/09107 Chemnitz/Allemagne (3 aut.); Technical Chemistry and Reaction Engineering, ITMC, RWTH Aachen, Worringerweg 1/52074 Aachen/Allemagne (4 aut.); CNRS-ENSIC, Laboratoire des Sciences du Genie Chimique, 1, rue Grandville/54001 Nancy/France (5 aut.)
DT :	Publication en série; Congrès; Niveau analytique
SO :	Chemical engineering journal : (1996); ISSN 1385-8947; Pays-Bas; Da. 2008; Vol. 135; No. SUP1; S89-S92; Bibl. 12 ref.
LA :	Anglais
EA :	The synthesis of optically pure cyanohydrins using a crude cell lysate containing the enzyme hydroxynitrile lyase (HNL) was studied in a microreactor in an aqueous-organic biphasic system. Different aldehyde substrates were selected to be converted to their corresponding cyanohydrins. It was successfully demonstrated that this crude cell lysate could readily be applied as a biocatalyst in a microchannel without clogging the channels. The biocatalytic activity toward the different substrates could rapidly be investigated with only small quantities of enzyme needed, compared to batch scale screening. Furthermore, the optimal contact between two immiscible phases in a microreactor resulted in enzymatic reactions with a high initial reaction rate and enantioselectivity, comparable to a batchwise process in which optimized conditions were achieved by vigorous stirring. Thus, performing the selected enzymatic reaction in a microreactor is a facile and cost efficient screening method leading to results which can be directly translated to batchwise processes.
CC :	001D07H; 002A31C09C; 215
FD :	Synthèse enzymatique; Microréacteur; Système biphasique; Biocatalyseur; En discontinu; Réaction enzymatique; Vitesse réaction; Cinétique; Enantiosélectivité; Agitation mécanique
ED :	Enzymatic synthesis; Microreactor; Biphasic system; Biocatalyst; Batchwise; Enzymatic reaction; Reaction rate; Kinetics; Enantioselectivity; Stirring
SD :	Síntesis enzimática; Microreactor; Sistema bifásico; Biocatalizador; En discontinuo; Reacción enzimática; Velocidad reacción; Cinética; Enantioselectividad; Agitación mecánica
LO :	INIST-14678.354000173568260120
ID :	08-0055156

Links to Exploration step

Pascal:08-0055156

Le document en format XML

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<front><div type="abstract" xml:lang="en">The synthesis of optically pure cyanohydrins using a crude cell lysate containing the enzyme hydroxynitrile lyase (HNL) was studied in a microreactor in an aqueous-organic biphasic system. Different aldehyde substrates were selected to be converted to their corresponding cyanohydrins. It was successfully demonstrated that this crude cell lysate could readily be applied as a biocatalyst in a microchannel without clogging the channels. The biocatalytic activity toward the different substrates could rapidly be investigated with only small quantities of enzyme needed, compared to batch scale screening. Furthermore, the optimal contact between two immiscible phases in a microreactor resulted in enzymatic reactions with a high initial reaction rate and enantioselectivity, comparable to a batchwise process in which optimized conditions were achieved by vigorous stirring. Thus, performing the selected enzymatic reaction in a microreactor is a facile and cost efficient screening method leading to results which can be directly translated to batchwise processes.</div>
</front>
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<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>1385-8947</s0>
</fA01>
<fA03 i2="1"><s0>Chem. eng. j. : (1996)</s0>
</fA03>
<fA05><s2>135</s2>
</fA05>
<fA06><s3>SUP1</s3>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate</s1>
</fA08>
<fA09 i1="01" i2="1" l="ENG"><s1>Microreaction Technology, IMRET 9: Proceedings of the Ninth International Conference on Microreaction Technology</s1>
</fA09>
<fA11 i1="01" i2="1"><s1>KOCH (Kaspar)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>VAN DEN BERG (Rutger J. F.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>NIEUWLAND (Pieter J.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>WIJTMANS (Roel)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>WUBBOLTS (Marcel G.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>SCHOEMAKER (Hans E.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>RUTJES (Floris P. J. T.)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>VAN HEST (Jan C. M.)</s1>
</fA11>
<fA12 i1="01" i2="1"><s1>SCHÜTTE (Rüdiger)</s1>
<s9>ed.</s9>
</fA12>
<fA12 i1="02" i2="1"><s1>RENKEN (Albert)</s1>
<s9>ed.</s9>
</fA12>
<fA12 i1="03" i2="1"><s1>KLEMM (Elias)</s1>
<s9>ed.</s9>
</fA12>
<fA12 i1="04" i2="1"><s1>LIAUW (Marcel A.)</s1>
<s9>ed.</s9>
</fA12>
<fA12 i1="05" i2="1"><s1>MATLOSZ (Michael)</s1>
<s9>ed.</s9>
</fA12>
<fA14 i1="01"><s1>Institute for Molecules and Materials, Radboud University Nijmegen, Toemooiveld 1</s1>
<s2>6525 ED Nijmegen</s2>
<s3>NLD</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>7 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>DSM Research, PO Box 18</s1>
<s2>6160 MD Geleen</s2>
<s3>NLD</s3>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA15 i1="01"><s1>Process Technology & Engineering, Evonik Degussa GmbH, Rodenbacher Chaussee 4</s1>
<s2>63457 Hanau (Wolfgang)</s2>
<s3>DEU</s3>
<sZ>1 aut.</sZ>
</fA15>
<fA15 i1="02"><s1>Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), SB-ISIC-LGRC-Station 6, Bâtiment CH / CH J2 500</s1>
<s2>1015 Lausanne</s2>
<s3>CHE</s3>
<sZ>2 aut.</sZ>
</fA15>
<fA15 i1="03"><s1>Chemnitz University of Technology, Faculty of Natural Sciences, Department of Chemical Technology</s1>
<s2>09107 Chemnitz</s2>
<s3>DEU</s3>
<sZ>3 aut.</sZ>
</fA15>
<fA15 i1="04"><s1>Technical Chemistry and Reaction Engineering, ITMC, RWTH Aachen, Worringerweg 1</s1>
<s2>52074 Aachen</s2>
<s3>DEU</s3>
<sZ>4 aut.</sZ>
</fA15>
<fA15 i1="05"><s1>CNRS-ENSIC, Laboratoire des Sciences du Genie Chimique, 1, rue Grandville</s1>
<s2>54001 Nancy</s2>
<s3>FRA</s3>
<sZ>5 aut.</sZ>
</fA15>
<fA20><s2>S89-S92</s2>
</fA20>
<fA21><s1>2008</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>14678</s2>
<s5>354000173568260120</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2008 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>12 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>08-0055156</s0>
</fA47>
<fA60><s1>P</s1>
<s2>C</s2>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Chemical engineering journal : (1996)</s0>
</fA64>
<fA66 i1="01"><s0>NLD</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The synthesis of optically pure cyanohydrins using a crude cell lysate containing the enzyme hydroxynitrile lyase (HNL) was studied in a microreactor in an aqueous-organic biphasic system. Different aldehyde substrates were selected to be converted to their corresponding cyanohydrins. It was successfully demonstrated that this crude cell lysate could readily be applied as a biocatalyst in a microchannel without clogging the channels. The biocatalytic activity toward the different substrates could rapidly be investigated with only small quantities of enzyme needed, compared to batch scale screening. Furthermore, the optimal contact between two immiscible phases in a microreactor resulted in enzymatic reactions with a high initial reaction rate and enantioselectivity, comparable to a batchwise process in which optimized conditions were achieved by vigorous stirring. Thus, performing the selected enzymatic reaction in a microreactor is a facile and cost efficient screening method leading to results which can be directly translated to batchwise processes.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>001D07H</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002A31C09C</s0>
</fC02>
<fC02 i1="03" i2="X"><s0>215</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Synthèse enzymatique</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Enzymatic synthesis</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Síntesis enzimática</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Microréacteur</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Microreactor</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Microreactor</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Système biphasique</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Biphasic system</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Sistema bifásico</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Biocatalyseur</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Biocatalyst</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Biocatalizador</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>En discontinu</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Batchwise</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>En discontinuo</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Réaction enzymatique</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Enzymatic reaction</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Reacción enzimática</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Vitesse réaction</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Reaction rate</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Velocidad reacción</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Cinétique</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Kinetics</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Cinética</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Enantiosélectivité</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Enantioselectivity</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Enantioselectividad</s0>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Agitation mécanique</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Stirring</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Agitación mecánica</s0>
<s5>10</s5>
</fC03>
<fN21><s1>028</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
<pR><fA30 i1="01" i2="1" l="ENG"><s1>IMRET 9 : International Conference on Microreaction Technology</s1>
<s2>9</s2>
<s3>Potsdam DEU</s3>
<s4>2006-09-06</s4>
</fA30>
</pR>
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<server><NO>PASCAL 08-0055156 INIST</NO>
<ET>Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate</ET>
<AU>KOCH (Kaspar); VAN DEN BERG (Rutger J. F.); NIEUWLAND (Pieter J.); WIJTMANS (Roel); WUBBOLTS (Marcel G.); SCHOEMAKER (Hans E.); RUTJES (Floris P. J. T.); VAN HEST (Jan C. M.); SCHÜTTE (Rüdiger); RENKEN (Albert); KLEMM (Elias); LIAUW (Marcel A.); MATLOSZ (Michael)</AU>
<AF>Institute for Molecules and Materials, Radboud University Nijmegen, Toemooiveld 1/6525 ED Nijmegen/Pays-Bas (1 aut., 2 aut., 3 aut., 4 aut., 7 aut., 8 aut.); DSM Research, PO Box 18/6160 MD Geleen/Pays-Bas (5 aut., 6 aut.); Process Technology & Engineering, Evonik Degussa GmbH, Rodenbacher Chaussee 4/63457 Hanau (Wolfgang)/Allemagne (1 aut.); Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), SB-ISIC-LGRC-Station 6, Bâtiment CH / CH J2 500/1015 Lausanne/Suisse (2 aut.); Chemnitz University of Technology, Faculty of Natural Sciences, Department of Chemical Technology/09107 Chemnitz/Allemagne (3 aut.); Technical Chemistry and Reaction Engineering, ITMC, RWTH Aachen, Worringerweg 1/52074 Aachen/Allemagne (4 aut.); CNRS-ENSIC, Laboratoire des Sciences du Genie Chimique, 1, rue Grandville/54001 Nancy/France (5 aut.)</AF>
<DT>Publication en série; Congrès; Niveau analytique</DT>
<SO>Chemical engineering journal : (1996); ISSN 1385-8947; Pays-Bas; Da. 2008; Vol. 135; No. SUP1; S89-S92; Bibl. 12 ref.</SO>
<LA>Anglais</LA>
<EA>The synthesis of optically pure cyanohydrins using a crude cell lysate containing the enzyme hydroxynitrile lyase (HNL) was studied in a microreactor in an aqueous-organic biphasic system. Different aldehyde substrates were selected to be converted to their corresponding cyanohydrins. It was successfully demonstrated that this crude cell lysate could readily be applied as a biocatalyst in a microchannel without clogging the channels. The biocatalytic activity toward the different substrates could rapidly be investigated with only small quantities of enzyme needed, compared to batch scale screening. Furthermore, the optimal contact between two immiscible phases in a microreactor resulted in enzymatic reactions with a high initial reaction rate and enantioselectivity, comparable to a batchwise process in which optimized conditions were achieved by vigorous stirring. Thus, performing the selected enzymatic reaction in a microreactor is a facile and cost efficient screening method leading to results which can be directly translated to batchwise processes.</EA>
<CC>001D07H; 002A31C09C; 215</CC>
<FD>Synthèse enzymatique; Microréacteur; Système biphasique; Biocatalyseur; En discontinu; Réaction enzymatique; Vitesse réaction; Cinétique; Enantiosélectivité; Agitation mécanique</FD>
<ED>Enzymatic synthesis; Microreactor; Biphasic system; Biocatalyst; Batchwise; Enzymatic reaction; Reaction rate; Kinetics; Enantioselectivity; Stirring</ED>
<SD>Síntesis enzimática; Microreactor; Sistema bifásico; Biocatalizador; En discontinuo; Reacción enzimática; Velocidad reacción; Cinética; Enantioselectividad; Agitación mecánica</SD>
<LO>INIST-14678.354000173568260120</LO>
<ID>08-0055156</ID>
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Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate

Enzymatic synthesis of optically pure cyanohydrins in microchannels using a crude cell lysate

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