UnivTrevesV1, PascalFrancis, Corpus, bibRecord, 000E80

Quality assurance in RT-PCR-based BCR/ABL diagnostics: results of an interlaboratory test and a standardization approach

Identifieur interne : 000E80 ( PascalFrancis/Corpus ); précédent : 000E79; suivant : 000E81

Quality assurance in RT-PCR-based BCR/ABL diagnostics: results of an interlaboratory test and a standardization approach

Auteurs : T. Burmeister ; J. Maurer ; M. Aivado ; A. H. Elmaagacli ; F. Grünebach ; K. R. Held ; G. Hess ; A. Hochhaus ; W. Höppner ; K. U. Lentes ; M. Lübbert ; K. L. Sch Fer ; P. Schafhausen ; C. A. Schmidt ; F. Schüler ; K. Seeger ; R. Seelig ; C. Thiede ; S. Viehmann ; C. Weber ; S. Wilhelm ; A. Christmann ; J. H. Clement ; U. Ebener ; J. Enczmann ; R. Leo ; M. Schleuning ; R. Schoch ; E. Thiel

Source :

Leukemia [ 0887-6924 ] ; 2000.

RBID : Pascal:01-0004051

Descripteurs français

Pascal (Inist)
- Translocation chromosomique, Chromosome C9 anormal, Chromosome G22 anormal, Gène hybride, Technique, Diagnostic, Assurance qualité, Expression génique, Réaction chaîne polymérase, RNA-directed DNA polymerase, Normalisation, Homme, Etude multicentrique, Gène bcr, Gène abl.

English descriptors

KwdEn :
- Abnormal chromosome C9, Abnormal chromosome G22, Chromosome translocation, Diagnosis, Gene expression, Human, Hybrid gene, Multicenter study, Polymerase chain reaction, Quality assurance, RNA-directed DNA polymerase, Standardization, Technique.

Abstract

Here we describe the results of an interlaboratory test for RT-PCR-based BCR/ABL analysis. The test was organized in two parts. The number of participating laboratories in the first and second part was 27 and 20, respectively. In the first part samples containing various concentrations of plasmids with the ela2, b2a2 or b3a2 BCR/ABL transcripts were analyzed by PCR. In the second part of the test, cell samples containing various concentrations of BCR/ABL-positive cells were analyzed by RT-PCR. Overall PCR sensitivity was sufficient in approximately 90% of the tests, but a significant number of false positive results were obtained. There were significant differences in sensitivity in the cell-based analysis between the various participants. The results are discussed, and proposals are made regarding the choice of primers, controls, conditions for RNA extraction and reverse transcription.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0887-6924`
A02	`01`			`@0 LEUKED`
A03		`1`		`@0 Leukemia`
A05				`@2 14`
A06				`@2 10`
A08	`01`	`1`	`ENG`	`@1 Quality assurance in RT-PCR-based BCR/ABL diagnostics: results of an interlaboratory test and a standardization approach`
A11	`01`	`1`		`@1 BURMEISTER (T.)`
A11	`02`	`1`		`@1 MAURER (J.)`
A11	`03`	`1`		`@1 AIVADO (M.)`
A11	`04`	`1`		`@1 ELMAAGACLI (A. H.)`
A11	`05`	`1`		`@1 GRÜNEBACH (F.)`
A11	`06`	`1`		`@1 HELD (K. R.)`
A11	`07`	`1`		`@1 HESS (G.)`
A11	`08`	`1`		`@1 HOCHHAUS (A.)`
A11	`09`	`1`		`@1 HÖPPNER (W.)`
A11	`10`	`1`		`@1 LENTES (K. U.)`
A11	`11`	`1`		`@1 LÜBBERT (M.)`
A11	`12`	`1`		`@1 SCHÄFER (K. L.)`
A11	`13`	`1`		`@1 SCHAFHAUSEN (P.)`
A11	`14`	`1`		`@1 SCHMIDT (C. A.)`
A11	`15`	`1`		`@1 SCHÜLER (F.)`
A11	`16`	`1`		`@1 SEEGER (K.)`
A11	`17`	`1`		`@1 SEELIG (R.)`
A11	`18`	`1`		`@1 THIEDE (C.)`
A11	`19`	`1`		`@1 VIEHMANN (S.)`
A11	`20`	`1`		`@1 WEBER (C.)`
A11	`21`	`1`		`@1 WILHELM (S.)`
A11	`22`	`1`		`@1 CHRISTMANN (A.)`
A11	`23`	`1`		`@1 CLEMENT (J. H.)`
A11	`24`	`1`		`@1 EBENER (U.)`
A11	`25`	`1`		`@1 ENCZMANN (J.)`
A11	`26`	`1`		`@1 LEO (R.)`
A11	`27`	`1`		`@1 SCHLEUNING (M.)`
A11	`28`	`1`		`@1 SCHOCH (R.)`
A11	`29`	`1`		`@1 THIEL (E.)`
A14	`01`			`@1 Freie Universitat Berlin, Med Klinik III @3 DEU @Z 1 aut. @Z 2 aut. @Z 29 aut.`
A14	`02`			`@1 Universität Düsseldorf, Klinik fur Hamatologie und Onkologie @3 DEU @Z 3 aut.`
A14	`03`			`@1 Universität-Gesamthochschule Essen, Klinik für Knochenmarktransplantation @3 DEU @Z 4 aut.`
A14	`04`			`@1 Universität Tübingen, Med, Klinik @3 DEU @Z 5 aut.`
A14	`05`			`@1 Praxis Held/Kesser/Arndt Hamburg @3 DEU @Z 6 aut.`
A14	`06`			`@1 Universität Mainz, III, Med, Klinik @3 DEU @Z 7 aut.`
A14	`07`			`@1 Universität Heidelberg, III, Med, Klinik, Klinikum Mannheim @3 DEU @Z 8 aut.`
A14	`08`			`@1 GLE medicon GmbH @2 Hamburg @3 DEU @Z 9 aut.`
A14	`09`			`@1 Universität Trier, Abt, Molekulare Neurogenetik @3 DEU @Z 10 aut.`
A14	`10`			`@1 Universität Freiburg, Med. Klinik I @3 DEU @Z 11 aut.`
A14	`11`			`@1 Universitat Münster, Institut fur Pathologie @3 DEU @Z 12 aut.`
A14	`12`			`@1 Universität Hamburg, Med. Klinik III @3 DEU @Z 13 aut.`
A14	`13`			`@1 ¹¹³Humboldt-Universitat Berlin, Med. Klinik, Hamatologie/Onkologie @3 DEU @Z 14 aut.`
A14	`14`			`@1 Universität Greifswald, Klinik fur Innere Medizin C @3 DEU @Z 15 aut.`
A14	`15`			`@1 Humboldt-Universitat Berlin, Zentrum fur Kinderheilkunde @3 DEU @Z 16 aut.`
A14	`16`			`@1 Labor Prof Seelig und Partner @2 Karlsruhe @3 DEU @Z 17 aut.`
A14	`17`			`@1 Technische Universitat Dresden, Med, Klinik 1 @3 DEU @Z 18 aut.`
A14	`18`			`@1 Universität Giessen, Kinderklinik, Onkogenetisches Labor @3 DEU @Z 19 aut.`
A14	`19`			`@1 Universität Giessen, Abt, for Hamatologie @3 DEU @Z 20 aut.`
A14	`20`			`@1 Universität Rostock, Abt für Hämatologie/Onkologie @3 DEU @Z 21 aut.`
A14	`21`			`@1 Universität Homburg, Institut für Humangenetik @3 DEU @Z 22 aut.`
A14	`22`			`@1 Universität Jena, Innere Medizin II @3 DEU @Z 23 aut.`
A14	`23`			`@1 Universität Frankfurt, Zentrum für Kinderheilkunde @3 DEU @Z 24 aut.`
A14	`24`			`@1 Universität Düsseldorf, Knochenmarkspenderzentrale @3 DEU @Z 25 aut.`
A14	`25`			`@1 Universität Münster, Med, Klinik A @3 DEU @Z 26 aut.`
A20				`@1 1850-1856`
A21				`@1 2000`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 21129 @5 354000092220510160`
A44				`@0 0000 @1 © 2001 INIST-CNRS. All rights reserved.`
A45				`@0 19 ref.`
A47	`01`	`1`		`@0 01-0004051`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Leukemia`
A66	`01`			`@0 GBR`
C01	`01`		`ENG`	@0 Here we describe the results of an interlaboratory test for RT-PCR-based BCR/ABL analysis. The test was organized in two parts. The number of participating laboratories in the first and second part was 27 and 20, respectively. In the first part samples containing various concentrations of plasmids with the ela2, b2a2 or b3a2 BCR/ABL transcripts were analyzed by PCR. In the second part of the test, cell samples containing various concentrations of BCR/ABL-positive cells were analyzed by RT-PCR. Overall PCR sensitivity was sufficient in approximately 90% of the tests, but a significant number of false positive results were obtained. There were significant differences in sensitivity in the cell-based analysis between the various participants. The results are discussed, and proposals are made regarding the choice of primers, controls, conditions for RNA extraction and reverse transcription.
C02	`01`	`X`		`@0 002B24O10`
C03	`01`	`X`	`FRE`	`@0 Translocation chromosomique @5 01`
C03	`01`	`X`	`ENG`	`@0 Chromosome translocation @5 01`
C03	`01`	`X`	`SPA`	`@0 Translocación cromosómica @5 01`
C03	`02`	`X`	`FRE`	`@0 Chromosome C9 anormal @5 02`
C03	`02`	`X`	`ENG`	`@0 Abnormal chromosome C9 @5 02`
C03	`02`	`X`	`SPA`	`@0 Cromosoma C9 anormal @5 02`
C03	`03`	`X`	`FRE`	`@0 Chromosome G22 anormal @5 03`
C03	`03`	`X`	`ENG`	`@0 Abnormal chromosome G22 @5 03`
C03	`03`	`X`	`SPA`	`@0 Cromosoma G22 anormal @5 03`
C03	`04`	`X`	`FRE`	`@0 Gène hybride @5 04`
C03	`04`	`X`	`ENG`	`@0 Hybrid gene @5 04`
C03	`04`	`X`	`SPA`	`@0 Gen híbrido @5 04`
C03	`05`	`X`	`FRE`	`@0 Technique @5 05`
C03	`05`	`X`	`ENG`	`@0 Technique @5 05`
C03	`05`	`X`	`SPA`	`@0 Técnica @5 05`
C03	`06`	`X`	`FRE`	`@0 Diagnostic @5 06`
C03	`06`	`X`	`ENG`	`@0 Diagnosis @5 06`
C03	`06`	`X`	`SPA`	`@0 Diagnóstico @5 06`
C03	`07`	`X`	`FRE`	`@0 Assurance qualité @5 08`
C03	`07`	`X`	`ENG`	`@0 Quality assurance @5 08`
C03	`07`	`X`	`SPA`	`@0 Aseguración calidad @5 08`
C03	`08`	`X`	`FRE`	`@0 Expression génique @5 09`
C03	`08`	`X`	`ENG`	`@0 Gene expression @5 09`
C03	`08`	`X`	`SPA`	`@0 Expresión genética @5 09`
C03	`09`	`X`	`FRE`	`@0 Réaction chaîne polymérase @5 10`
C03	`09`	`X`	`ENG`	`@0 Polymerase chain reaction @5 10`
C03	`09`	`X`	`SPA`	`@0 Reacción cadena polimerasa @5 10`
C03	`10`	`X`	`FRE`	`@0 RNA-directed DNA polymerase @2 FE @5 11`
C03	`10`	`X`	`ENG`	`@0 RNA-directed DNA polymerase @2 FE @5 11`
C03	`10`	`X`	`SPA`	`@0 RNA-directed DNA polymerase @2 FE @5 11`
C03	`11`	`X`	`FRE`	`@0 Normalisation @5 18`
C03	`11`	`X`	`ENG`	`@0 Standardization @5 18`
C03	`11`	`X`	`SPA`	`@0 Normalización @5 18`
C03	`12`	`X`	`FRE`	`@0 Homme @5 19`
C03	`12`	`X`	`ENG`	`@0 Human @5 19`
C03	`12`	`X`	`SPA`	`@0 Hombre @5 19`
C03	`13`	`X`	`FRE`	`@0 Etude multicentrique @5 20`
C03	`13`	`X`	`ENG`	`@0 Multicenter study @5 20`
C03	`13`	`X`	`SPA`	`@0 Estudio multicéntrico @5 20`
C03	`14`	`X`	`FRE`	`@0 Gène bcr @4 INC @5 86`
C03	`15`	`X`	`FRE`	`@0 Gène abl @4 INC @5 87`
C07	`01`	`X`	`FRE`	`@0 Chromosome anormal`
C07	`01`	`X`	`ENG`	`@0 Abnormal chromosome`
C07	`01`	`X`	`SPA`	`@0 Cromosoma anormal`
C07	`02`	`X`	`FRE`	`@0 Aberration chromosomique`
C07	`02`	`X`	`ENG`	`@0 Chromosomal aberration`
C07	`02`	`X`	`SPA`	`@0 Aberración cromosómica`
C07	`03`	`X`	`FRE`	`@0 Nucleotidyltransferases @2 FE`
C07	`03`	`X`	`ENG`	`@0 Nucleotidyltransferases @2 FE`
C07	`03`	`X`	`SPA`	`@0 Nucleotidyltransferases @2 FE`
C07	`04`	`X`	`FRE`	`@0 Transferases @2 FE`
C07	`04`	`X`	`ENG`	`@0 Transferases @2 FE`
C07	`04`	`X`	`SPA`	`@0 Transferases @2 FE`
C07	`05`	`X`	`FRE`	`@0 Enzyme`
C07	`05`	`X`	`ENG`	`@0 Enzyme`
C07	`05`	`X`	`SPA`	`@0 Enzima`
C07	`06`	`X`	`FRE`	`@0 Biologie moléculaire @5 61`
C07	`06`	`X`	`ENG`	`@0 Molecular biology @5 61`
C07	`06`	`X`	`SPA`	`@0 Biología molecular @5 61`
N21				`@1 001`

Format Inist (serveur)

NO :	PASCAL 01-0004051 INIST
ET :	Quality assurance in RT-PCR-based BCR/ABL diagnostics: results of an interlaboratory test and a standardization approach
AU :	BURMEISTER (T.); MAURER (J.); AIVADO (M.); ELMAAGACLI (A. H.); GRÜNEBACH (F.); HELD (K. R.); HESS (G.); HOCHHAUS (A.); HÖPPNER (W.); LENTES (K. U.); LÜBBERT (M.); SCHÄFER (K. L.); SCHAFHAUSEN (P.); SCHMIDT (C. A.); SCHÜLER (F.); SEEGER (K.); SEELIG (R.); THIEDE (C.); VIEHMANN (S.); WEBER (C.); WILHELM (S.); CHRISTMANN (A.); CLEMENT (J. H.); EBENER (U.); ENCZMANN (J.); LEO (R.); SCHLEUNING (M.); SCHOCH (R.); THIEL (E.)
AF :	Freie Universitat Berlin, Med Klinik III/Allemagne (1 aut., 2 aut., 29 aut.); Universität Düsseldorf, Klinik fur Hamatologie und Onkologie/Allemagne (3 aut.); Universität-Gesamthochschule Essen, Klinik für Knochenmarktransplantation/Allemagne (4 aut.); Universität Tübingen, Med, Klinik/Allemagne (5 aut.); Praxis Held/Kesser/Arndt Hamburg/Allemagne (6 aut.); Universität Mainz, III, Med, Klinik/Allemagne (7 aut.); Universität Heidelberg, III, Med, Klinik, Klinikum Mannheim/Allemagne (8 aut.); GLE medicon GmbH/Hamburg/Allemagne (9 aut.); Universität Trier, Abt, Molekulare Neurogenetik/Allemagne (10 aut.); Universität Freiburg, Med. Klinik I/Allemagne (11 aut.); Universitat Münster, Institut fur Pathologie/Allemagne (12 aut.); Universität Hamburg, Med. Klinik III/Allemagne (13 aut.); ¹¹³Humboldt-Universitat Berlin, Med. Klinik, Hamatologie/Onkologie/Allemagne (14 aut.); Universität Greifswald, Klinik fur Innere Medizin C/Allemagne (15 aut.); Humboldt-Universitat Berlin, Zentrum fur Kinderheilkunde/Allemagne (16 aut.); Labor Prof Seelig und Partner/Karlsruhe/Allemagne (17 aut.); Technische Universitat Dresden, Med, Klinik 1/Allemagne (18 aut.); Universität Giessen, Kinderklinik, Onkogenetisches Labor/Allemagne (19 aut.); Universität Giessen, Abt, for Hamatologie/Allemagne (20 aut.); Universität Rostock, Abt für Hämatologie/Onkologie/Allemagne (21 aut.); Universität Homburg, Institut für Humangenetik/Allemagne (22 aut.); Universität Jena, Innere Medizin II/Allemagne (23 aut.); Universität Frankfurt, Zentrum für Kinderheilkunde/Allemagne (24 aut.); Universität Düsseldorf, Knochenmarkspenderzentrale/Allemagne (25 aut.); Universität Münster, Med, Klinik A/Allemagne (26 aut.)
DT :	Publication en série; Niveau analytique
SO :	Leukemia; ISSN 0887-6924; Coden LEUKED; Royaume-Uni; Da. 2000; Vol. 14; No. 10; Pp. 1850-1856; Bibl. 19 ref.
LA :	Anglais
EA :	Here we describe the results of an interlaboratory test for RT-PCR-based BCR/ABL analysis. The test was organized in two parts. The number of participating laboratories in the first and second part was 27 and 20, respectively. In the first part samples containing various concentrations of plasmids with the ela2, b2a2 or b3a2 BCR/ABL transcripts were analyzed by PCR. In the second part of the test, cell samples containing various concentrations of BCR/ABL-positive cells were analyzed by RT-PCR. Overall PCR sensitivity was sufficient in approximately 90% of the tests, but a significant number of false positive results were obtained. There were significant differences in sensitivity in the cell-based analysis between the various participants. The results are discussed, and proposals are made regarding the choice of primers, controls, conditions for RNA extraction and reverse transcription.
CC :	002B24O10
FD :	Translocation chromosomique; Chromosome C9 anormal; Chromosome G22 anormal; Gène hybride; Technique; Diagnostic; Assurance qualité; Expression génique; Réaction chaîne polymérase; RNA-directed DNA polymerase; Normalisation; Homme; Etude multicentrique; Gène bcr; Gène abl
FG :	Chromosome anormal; Aberration chromosomique; Nucleotidyltransferases; Transferases; Enzyme; Biologie moléculaire
ED :	Chromosome translocation; Abnormal chromosome C9; Abnormal chromosome G22; Hybrid gene; Technique; Diagnosis; Quality assurance; Gene expression; Polymerase chain reaction; RNA-directed DNA polymerase; Standardization; Human; Multicenter study
EG :	Abnormal chromosome; Chromosomal aberration; Nucleotidyltransferases; Transferases; Enzyme; Molecular biology
SD :	Translocación cromosómica; Cromosoma C9 anormal; Cromosoma G22 anormal; Gen híbrido; Técnica; Diagnóstico; Aseguración calidad; Expresión genética; Reacción cadena polimerasa; RNA-directed DNA polymerase; Normalización; Hombre; Estudio multicéntrico
LO :	INIST-21129.354000092220510160
ID :	01-0004051

Links to Exploration step

Pascal:01-0004051

Le document en format XML

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<author><name sortKey="Hochhaus, A" sort="Hochhaus, A" uniqKey="Hochhaus A" first="A." last="Hochhaus">A. Hochhaus</name>
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<author><name sortKey="Lubbert, M" sort="Lubbert, M" uniqKey="Lubbert M" first="M." last="Lübbert">M. Lübbert</name>
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<author><name sortKey="Sch Fer, K L" sort="Sch Fer, K L" uniqKey="Sch Fer K" first="K. L." last="Sch Fer">K. L. Sch Fer</name>
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<author><name sortKey="Schafhausen, P" sort="Schafhausen, P" uniqKey="Schafhausen P" first="P." last="Schafhausen">P. Schafhausen</name>
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<author><name sortKey="Schmidt, C A" sort="Schmidt, C A" uniqKey="Schmidt C" first="C. A." last="Schmidt">C. A. Schmidt</name>
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<author><name sortKey="Schuler, F" sort="Schuler, F" uniqKey="Schuler F" first="F." last="Schüler">F. Schüler</name>
<affiliation><inist:fA14 i1="14"><s1>Universität Greifswald, Klinik fur Innere Medizin C</s1>
<s3>DEU</s3>
<sZ>15 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Seeger, K" sort="Seeger, K" uniqKey="Seeger K" first="K." last="Seeger">K. Seeger</name>
<affiliation><inist:fA14 i1="15"><s1>Humboldt-Universitat Berlin, Zentrum fur Kinderheilkunde</s1>
<s3>DEU</s3>
<sZ>16 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Seelig, R" sort="Seelig, R" uniqKey="Seelig R" first="R." last="Seelig">R. Seelig</name>
<affiliation><inist:fA14 i1="16"><s1>Labor Prof Seelig und Partner</s1>
<s2>Karlsruhe</s2>
<s3>DEU</s3>
<sZ>17 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Thiede, C" sort="Thiede, C" uniqKey="Thiede C" first="C." last="Thiede">C. Thiede</name>
<affiliation><inist:fA14 i1="17"><s1>Technische Universitat Dresden, Med, Klinik 1</s1>
<s3>DEU</s3>
<sZ>18 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Viehmann, S" sort="Viehmann, S" uniqKey="Viehmann S" first="S." last="Viehmann">S. Viehmann</name>
<affiliation><inist:fA14 i1="18"><s1>Universität Giessen, Kinderklinik, Onkogenetisches Labor</s1>
<s3>DEU</s3>
<sZ>19 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Weber, C" sort="Weber, C" uniqKey="Weber C" first="C." last="Weber">C. Weber</name>
<affiliation><inist:fA14 i1="19"><s1>Universität Giessen, Abt, for Hamatologie</s1>
<s3>DEU</s3>
<sZ>20 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Wilhelm, S" sort="Wilhelm, S" uniqKey="Wilhelm S" first="S." last="Wilhelm">S. Wilhelm</name>
<affiliation><inist:fA14 i1="20"><s1>Universität Rostock, Abt für Hämatologie/Onkologie</s1>
<s3>DEU</s3>
<sZ>21 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Christmann, A" sort="Christmann, A" uniqKey="Christmann A" first="A." last="Christmann">A. Christmann</name>
<affiliation><inist:fA14 i1="21"><s1>Universität Homburg, Institut für Humangenetik</s1>
<s3>DEU</s3>
<sZ>22 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Clement, J H" sort="Clement, J H" uniqKey="Clement J" first="J. H." last="Clement">J. H. Clement</name>
<affiliation><inist:fA14 i1="22"><s1>Universität Jena, Innere Medizin II</s1>
<s3>DEU</s3>
<sZ>23 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Ebener, U" sort="Ebener, U" uniqKey="Ebener U" first="U." last="Ebener">U. Ebener</name>
<affiliation><inist:fA14 i1="23"><s1>Universität Frankfurt, Zentrum für Kinderheilkunde</s1>
<s3>DEU</s3>
<sZ>24 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Enczmann, J" sort="Enczmann, J" uniqKey="Enczmann J" first="J." last="Enczmann">J. Enczmann</name>
<affiliation><inist:fA14 i1="24"><s1>Universität Düsseldorf, Knochenmarkspenderzentrale</s1>
<s3>DEU</s3>
<sZ>25 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Leo, R" sort="Leo, R" uniqKey="Leo R" first="R." last="Leo">R. Leo</name>
<affiliation><inist:fA14 i1="25"><s1>Universität Münster, Med, Klinik A</s1>
<s3>DEU</s3>
<sZ>26 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Schleuning, M" sort="Schleuning, M" uniqKey="Schleuning M" first="M." last="Schleuning">M. Schleuning</name>
</author>
<author><name sortKey="Schoch, R" sort="Schoch, R" uniqKey="Schoch R" first="R." last="Schoch">R. Schoch</name>
</author>
<author><name sortKey="Thiel, E" sort="Thiel, E" uniqKey="Thiel E" first="E." last="Thiel">E. Thiel</name>
<affiliation><inist:fA14 i1="01"><s1>Freie Universitat Berlin, Med Klinik III</s1>
<s3>DEU</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>29 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">01-0004051</idno>
<date when="2000">2000</date>
<idno type="stanalyst">PASCAL 01-0004051 INIST</idno>
<idno type="RBID">Pascal:01-0004051</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000E80</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Quality assurance in RT-PCR-based BCR/ABL diagnostics: results of an interlaboratory test and a standardization approach</title>
<author><name sortKey="Burmeister, T" sort="Burmeister, T" uniqKey="Burmeister T" first="T." last="Burmeister">T. Burmeister</name>
<affiliation><inist:fA14 i1="01"><s1>Freie Universitat Berlin, Med Klinik III</s1>
<s3>DEU</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>29 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Maurer, J" sort="Maurer, J" uniqKey="Maurer J" first="J." last="Maurer">J. Maurer</name>
<affiliation><inist:fA14 i1="01"><s1>Freie Universitat Berlin, Med Klinik III</s1>
<s3>DEU</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>29 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Aivado, M" sort="Aivado, M" uniqKey="Aivado M" first="M." last="Aivado">M. Aivado</name>
<affiliation><inist:fA14 i1="02"><s1>Universität Düsseldorf, Klinik fur Hamatologie und Onkologie</s1>
<s3>DEU</s3>
<sZ>3 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Elmaagacli, A H" sort="Elmaagacli, A H" uniqKey="Elmaagacli A" first="A. H." last="Elmaagacli">A. H. Elmaagacli</name>
<affiliation><inist:fA14 i1="03"><s1>Universität-Gesamthochschule Essen, Klinik für Knochenmarktransplantation</s1>
<s3>DEU</s3>
<sZ>4 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Grunebach, F" sort="Grunebach, F" uniqKey="Grunebach F" first="F." last="Grünebach">F. Grünebach</name>
<affiliation><inist:fA14 i1="04"><s1>Universität Tübingen, Med, Klinik</s1>
<s3>DEU</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Held, K R" sort="Held, K R" uniqKey="Held K" first="K. R." last="Held">K. R. Held</name>
<affiliation><inist:fA14 i1="05"><s1>Praxis Held/Kesser/Arndt Hamburg</s1>
<s3>DEU</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Hess, G" sort="Hess, G" uniqKey="Hess G" first="G." last="Hess">G. Hess</name>
<affiliation><inist:fA14 i1="06"><s1>Universität Mainz, III, Med, Klinik</s1>
<s3>DEU</s3>
<sZ>7 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Hochhaus, A" sort="Hochhaus, A" uniqKey="Hochhaus A" first="A." last="Hochhaus">A. Hochhaus</name>
<affiliation><inist:fA14 i1="07"><s1>Universität Heidelberg, III, Med, Klinik, Klinikum Mannheim</s1>
<s3>DEU</s3>
<sZ>8 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Hoppner, W" sort="Hoppner, W" uniqKey="Hoppner W" first="W." last="Höppner">W. Höppner</name>
<affiliation><inist:fA14 i1="08"><s1>GLE medicon GmbH</s1>
<s2>Hamburg</s2>
<s3>DEU</s3>
<sZ>9 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Lentes, K U" sort="Lentes, K U" uniqKey="Lentes K" first="K. U." last="Lentes">K. U. Lentes</name>
<affiliation><inist:fA14 i1="09"><s1>Universität Trier, Abt, Molekulare Neurogenetik</s1>
<s3>DEU</s3>
<sZ>10 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Lubbert, M" sort="Lubbert, M" uniqKey="Lubbert M" first="M." last="Lübbert">M. Lübbert</name>
<affiliation><inist:fA14 i1="10"><s1>Universität Freiburg, Med. Klinik I</s1>
<s3>DEU</s3>
<sZ>11 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Sch Fer, K L" sort="Sch Fer, K L" uniqKey="Sch Fer K" first="K. L." last="Sch Fer">K. L. Sch Fer</name>
<affiliation><inist:fA14 i1="11"><s1>Universitat Münster, Institut fur Pathologie</s1>
<s3>DEU</s3>
<sZ>12 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Schafhausen, P" sort="Schafhausen, P" uniqKey="Schafhausen P" first="P." last="Schafhausen">P. Schafhausen</name>
<affiliation><inist:fA14 i1="12"><s1>Universität Hamburg, Med. Klinik III</s1>
<s3>DEU</s3>
<sZ>13 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Schmidt, C A" sort="Schmidt, C A" uniqKey="Schmidt C" first="C. A." last="Schmidt">C. A. Schmidt</name>
<affiliation><inist:fA14 i1="13"><s1><sup>113</sup>
Humboldt-Universitat Berlin, Med. Klinik, Hamatologie/Onkologie</s1>
<s3>DEU</s3>
<sZ>14 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Schuler, F" sort="Schuler, F" uniqKey="Schuler F" first="F." last="Schüler">F. Schüler</name>
<affiliation><inist:fA14 i1="14"><s1>Universität Greifswald, Klinik fur Innere Medizin C</s1>
<s3>DEU</s3>
<sZ>15 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Seeger, K" sort="Seeger, K" uniqKey="Seeger K" first="K." last="Seeger">K. Seeger</name>
<affiliation><inist:fA14 i1="15"><s1>Humboldt-Universitat Berlin, Zentrum fur Kinderheilkunde</s1>
<s3>DEU</s3>
<sZ>16 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Seelig, R" sort="Seelig, R" uniqKey="Seelig R" first="R." last="Seelig">R. Seelig</name>
<affiliation><inist:fA14 i1="16"><s1>Labor Prof Seelig und Partner</s1>
<s2>Karlsruhe</s2>
<s3>DEU</s3>
<sZ>17 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Thiede, C" sort="Thiede, C" uniqKey="Thiede C" first="C." last="Thiede">C. Thiede</name>
<affiliation><inist:fA14 i1="17"><s1>Technische Universitat Dresden, Med, Klinik 1</s1>
<s3>DEU</s3>
<sZ>18 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Viehmann, S" sort="Viehmann, S" uniqKey="Viehmann S" first="S." last="Viehmann">S. Viehmann</name>
<affiliation><inist:fA14 i1="18"><s1>Universität Giessen, Kinderklinik, Onkogenetisches Labor</s1>
<s3>DEU</s3>
<sZ>19 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Weber, C" sort="Weber, C" uniqKey="Weber C" first="C." last="Weber">C. Weber</name>
<affiliation><inist:fA14 i1="19"><s1>Universität Giessen, Abt, for Hamatologie</s1>
<s3>DEU</s3>
<sZ>20 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Wilhelm, S" sort="Wilhelm, S" uniqKey="Wilhelm S" first="S." last="Wilhelm">S. Wilhelm</name>
<affiliation><inist:fA14 i1="20"><s1>Universität Rostock, Abt für Hämatologie/Onkologie</s1>
<s3>DEU</s3>
<sZ>21 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Christmann, A" sort="Christmann, A" uniqKey="Christmann A" first="A." last="Christmann">A. Christmann</name>
<affiliation><inist:fA14 i1="21"><s1>Universität Homburg, Institut für Humangenetik</s1>
<s3>DEU</s3>
<sZ>22 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Clement, J H" sort="Clement, J H" uniqKey="Clement J" first="J. H." last="Clement">J. H. Clement</name>
<affiliation><inist:fA14 i1="22"><s1>Universität Jena, Innere Medizin II</s1>
<s3>DEU</s3>
<sZ>23 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Ebener, U" sort="Ebener, U" uniqKey="Ebener U" first="U." last="Ebener">U. Ebener</name>
<affiliation><inist:fA14 i1="23"><s1>Universität Frankfurt, Zentrum für Kinderheilkunde</s1>
<s3>DEU</s3>
<sZ>24 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Enczmann, J" sort="Enczmann, J" uniqKey="Enczmann J" first="J." last="Enczmann">J. Enczmann</name>
<affiliation><inist:fA14 i1="24"><s1>Universität Düsseldorf, Knochenmarkspenderzentrale</s1>
<s3>DEU</s3>
<sZ>25 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Leo, R" sort="Leo, R" uniqKey="Leo R" first="R." last="Leo">R. Leo</name>
<affiliation><inist:fA14 i1="25"><s1>Universität Münster, Med, Klinik A</s1>
<s3>DEU</s3>
<sZ>26 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Schleuning, M" sort="Schleuning, M" uniqKey="Schleuning M" first="M." last="Schleuning">M. Schleuning</name>
</author>
<author><name sortKey="Schoch, R" sort="Schoch, R" uniqKey="Schoch R" first="R." last="Schoch">R. Schoch</name>
</author>
<author><name sortKey="Thiel, E" sort="Thiel, E" uniqKey="Thiel E" first="E." last="Thiel">E. Thiel</name>
<affiliation><inist:fA14 i1="01"><s1>Freie Universitat Berlin, Med Klinik III</s1>
<s3>DEU</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>29 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Leukemia</title>
<title level="j" type="abbreviated">Leukemia</title>
<idno type="ISSN">0887-6924</idno>
<imprint><date when="2000">2000</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Leukemia</title>
<title level="j" type="abbreviated">Leukemia</title>
<idno type="ISSN">0887-6924</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Abnormal chromosome C9</term>
<term>Abnormal chromosome G22</term>
<term>Chromosome translocation</term>
<term>Diagnosis</term>
<term>Gene expression</term>
<term>Human</term>
<term>Hybrid gene</term>
<term>Multicenter study</term>
<term>Polymerase chain reaction</term>
<term>Quality assurance</term>
<term>RNA-directed DNA polymerase</term>
<term>Standardization</term>
<term>Technique</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Translocation chromosomique</term>
<term>Chromosome C9 anormal</term>
<term>Chromosome G22 anormal</term>
<term>Gène hybride</term>
<term>Technique</term>
<term>Diagnostic</term>
<term>Assurance qualité</term>
<term>Expression génique</term>
<term>Réaction chaîne polymérase</term>
<term>RNA-directed DNA polymerase</term>
<term>Normalisation</term>
<term>Homme</term>
<term>Etude multicentrique</term>
<term>Gène bcr</term>
<term>Gène abl</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Here we describe the results of an interlaboratory test for RT-PCR-based BCR/ABL analysis. The test was organized in two parts. The number of participating laboratories in the first and second part was 27 and 20, respectively. In the first part samples containing various concentrations of plasmids with the ela2, b2a2 or b3a2 BCR/ABL transcripts were analyzed by PCR. In the second part of the test, cell samples containing various concentrations of BCR/ABL-positive cells were analyzed by RT-PCR. Overall PCR sensitivity was sufficient in approximately 90% of the tests, but a significant number of false positive results were obtained. There were significant differences in sensitivity in the cell-based analysis between the various participants. The results are discussed, and proposals are made regarding the choice of primers, controls, conditions for RNA extraction and reverse transcription.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0887-6924</s0>
</fA01>
<fA02 i1="01"><s0>LEUKED</s0>
</fA02>
<fA03 i2="1"><s0>Leukemia</s0>
</fA03>
<fA05><s2>14</s2>
</fA05>
<fA06><s2>10</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Quality assurance in RT-PCR-based BCR/ABL diagnostics: results of an interlaboratory test and a standardization approach</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>BURMEISTER (T.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>MAURER (J.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>AIVADO (M.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>ELMAAGACLI (A. H.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>GRÜNEBACH (F.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>HELD (K. R.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>HESS (G.)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>HOCHHAUS (A.)</s1>
</fA11>
<fA11 i1="09" i2="1"><s1>HÖPPNER (W.)</s1>
</fA11>
<fA11 i1="10" i2="1"><s1>LENTES (K. U.)</s1>
</fA11>
<fA11 i1="11" i2="1"><s1>LÜBBERT (M.)</s1>
</fA11>
<fA11 i1="12" i2="1"><s1>SCHÄFER (K. L.)</s1>
</fA11>
<fA11 i1="13" i2="1"><s1>SCHAFHAUSEN (P.)</s1>
</fA11>
<fA11 i1="14" i2="1"><s1>SCHMIDT (C. A.)</s1>
</fA11>
<fA11 i1="15" i2="1"><s1>SCHÜLER (F.)</s1>
</fA11>
<fA11 i1="16" i2="1"><s1>SEEGER (K.)</s1>
</fA11>
<fA11 i1="17" i2="1"><s1>SEELIG (R.)</s1>
</fA11>
<fA11 i1="18" i2="1"><s1>THIEDE (C.)</s1>
</fA11>
<fA11 i1="19" i2="1"><s1>VIEHMANN (S.)</s1>
</fA11>
<fA11 i1="20" i2="1"><s1>WEBER (C.)</s1>
</fA11>
<fA11 i1="21" i2="1"><s1>WILHELM (S.)</s1>
</fA11>
<fA11 i1="22" i2="1"><s1>CHRISTMANN (A.)</s1>
</fA11>
<fA11 i1="23" i2="1"><s1>CLEMENT (J. H.)</s1>
</fA11>
<fA11 i1="24" i2="1"><s1>EBENER (U.)</s1>
</fA11>
<fA11 i1="25" i2="1"><s1>ENCZMANN (J.)</s1>
</fA11>
<fA11 i1="26" i2="1"><s1>LEO (R.)</s1>
</fA11>
<fA11 i1="27" i2="1"><s1>SCHLEUNING (M.)</s1>
</fA11>
<fA11 i1="28" i2="1"><s1>SCHOCH (R.)</s1>
</fA11>
<fA11 i1="29" i2="1"><s1>THIEL (E.)</s1>
</fA11>
<fA14 i1="01"><s1>Freie Universitat Berlin, Med Klinik III</s1>
<s3>DEU</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>29 aut.</sZ>
</fA14>
<fA14 i1="02"><s1>Universität Düsseldorf, Klinik fur Hamatologie und Onkologie</s1>
<s3>DEU</s3>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="03"><s1>Universität-Gesamthochschule Essen, Klinik für Knochenmarktransplantation</s1>
<s3>DEU</s3>
<sZ>4 aut.</sZ>
</fA14>
<fA14 i1="04"><s1>Universität Tübingen, Med, Klinik</s1>
<s3>DEU</s3>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="05"><s1>Praxis Held/Kesser/Arndt Hamburg</s1>
<s3>DEU</s3>
<sZ>6 aut.</sZ>
</fA14>
<fA14 i1="06"><s1>Universität Mainz, III, Med, Klinik</s1>
<s3>DEU</s3>
<sZ>7 aut.</sZ>
</fA14>
<fA14 i1="07"><s1>Universität Heidelberg, III, Med, Klinik, Klinikum Mannheim</s1>
<s3>DEU</s3>
<sZ>8 aut.</sZ>
</fA14>
<fA14 i1="08"><s1>GLE medicon GmbH</s1>
<s2>Hamburg</s2>
<s3>DEU</s3>
<sZ>9 aut.</sZ>
</fA14>
<fA14 i1="09"><s1>Universität Trier, Abt, Molekulare Neurogenetik</s1>
<s3>DEU</s3>
<sZ>10 aut.</sZ>
</fA14>
<fA14 i1="10"><s1>Universität Freiburg, Med. Klinik I</s1>
<s3>DEU</s3>
<sZ>11 aut.</sZ>
</fA14>
<fA14 i1="11"><s1>Universitat Münster, Institut fur Pathologie</s1>
<s3>DEU</s3>
<sZ>12 aut.</sZ>
</fA14>
<fA14 i1="12"><s1>Universität Hamburg, Med. Klinik III</s1>
<s3>DEU</s3>
<sZ>13 aut.</sZ>
</fA14>
<fA14 i1="13"><s1><sup>113</sup>
Humboldt-Universitat Berlin, Med. Klinik, Hamatologie/Onkologie</s1>
<s3>DEU</s3>
<sZ>14 aut.</sZ>
</fA14>
<fA14 i1="14"><s1>Universität Greifswald, Klinik fur Innere Medizin C</s1>
<s3>DEU</s3>
<sZ>15 aut.</sZ>
</fA14>
<fA14 i1="15"><s1>Humboldt-Universitat Berlin, Zentrum fur Kinderheilkunde</s1>
<s3>DEU</s3>
<sZ>16 aut.</sZ>
</fA14>
<fA14 i1="16"><s1>Labor Prof Seelig und Partner</s1>
<s2>Karlsruhe</s2>
<s3>DEU</s3>
<sZ>17 aut.</sZ>
</fA14>
<fA14 i1="17"><s1>Technische Universitat Dresden, Med, Klinik 1</s1>
<s3>DEU</s3>
<sZ>18 aut.</sZ>
</fA14>
<fA14 i1="18"><s1>Universität Giessen, Kinderklinik, Onkogenetisches Labor</s1>
<s3>DEU</s3>
<sZ>19 aut.</sZ>
</fA14>
<fA14 i1="19"><s1>Universität Giessen, Abt, for Hamatologie</s1>
<s3>DEU</s3>
<sZ>20 aut.</sZ>
</fA14>
<fA14 i1="20"><s1>Universität Rostock, Abt für Hämatologie/Onkologie</s1>
<s3>DEU</s3>
<sZ>21 aut.</sZ>
</fA14>
<fA14 i1="21"><s1>Universität Homburg, Institut für Humangenetik</s1>
<s3>DEU</s3>
<sZ>22 aut.</sZ>
</fA14>
<fA14 i1="22"><s1>Universität Jena, Innere Medizin II</s1>
<s3>DEU</s3>
<sZ>23 aut.</sZ>
</fA14>
<fA14 i1="23"><s1>Universität Frankfurt, Zentrum für Kinderheilkunde</s1>
<s3>DEU</s3>
<sZ>24 aut.</sZ>
</fA14>
<fA14 i1="24"><s1>Universität Düsseldorf, Knochenmarkspenderzentrale</s1>
<s3>DEU</s3>
<sZ>25 aut.</sZ>
</fA14>
<fA14 i1="25"><s1>Universität Münster, Med, Klinik A</s1>
<s3>DEU</s3>
<sZ>26 aut.</sZ>
</fA14>
<fA20><s1>1850-1856</s1>
</fA20>
<fA21><s1>2000</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>21129</s2>
<s5>354000092220510160</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2001 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>19 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>01-0004051</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Leukemia</s0>
</fA64>
<fA66 i1="01"><s0>GBR</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>Here we describe the results of an interlaboratory test for RT-PCR-based BCR/ABL analysis. The test was organized in two parts. The number of participating laboratories in the first and second part was 27 and 20, respectively. In the first part samples containing various concentrations of plasmids with the ela2, b2a2 or b3a2 BCR/ABL transcripts were analyzed by PCR. In the second part of the test, cell samples containing various concentrations of BCR/ABL-positive cells were analyzed by RT-PCR. Overall PCR sensitivity was sufficient in approximately 90% of the tests, but a significant number of false positive results were obtained. There were significant differences in sensitivity in the cell-based analysis between the various participants. The results are discussed, and proposals are made regarding the choice of primers, controls, conditions for RNA extraction and reverse transcription.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002B24O10</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Translocation chromosomique</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Chromosome translocation</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Translocación cromosómica</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Chromosome C9 anormal</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Abnormal chromosome C9</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Cromosoma C9 anormal</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Chromosome G22 anormal</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Abnormal chromosome G22</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Cromosoma G22 anormal</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Gène hybride</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Hybrid gene</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Gen híbrido</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Technique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Technique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Técnica</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Diagnostic</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Diagnosis</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Diagnóstico</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Assurance qualité</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Quality assurance</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Aseguración calidad</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Expression génique</s0>
<s5>09</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Gene expression</s0>
<s5>09</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Expresión genética</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Réaction chaîne polymérase</s0>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Polymerase chain reaction</s0>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Reacción cadena polimerasa</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>RNA-directed DNA polymerase</s0>
<s2>FE</s2>
<s5>11</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>RNA-directed DNA polymerase</s0>
<s2>FE</s2>
<s5>11</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>RNA-directed DNA polymerase</s0>
<s2>FE</s2>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Normalisation</s0>
<s5>18</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Standardization</s0>
<s5>18</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Normalización</s0>
<s5>18</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Homme</s0>
<s5>19</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Human</s0>
<s5>19</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Hombre</s0>
<s5>19</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Etude multicentrique</s0>
<s5>20</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Multicenter study</s0>
<s5>20</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Estudio multicéntrico</s0>
<s5>20</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Gène bcr</s0>
<s4>INC</s4>
<s5>86</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Gène abl</s0>
<s4>INC</s4>
<s5>87</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Chromosome anormal</s0>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Abnormal chromosome</s0>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Cromosoma anormal</s0>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Aberration chromosomique</s0>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Chromosomal aberration</s0>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Aberración cromosómica</s0>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Nucleotidyltransferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Transferases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Enzyme</s0>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Enzyme</s0>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Enzima</s0>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Biologie moléculaire</s0>
<s5>61</s5>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Molecular biology</s0>
<s5>61</s5>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Biología molecular</s0>
<s5>61</s5>
</fC07>
<fN21><s1>001</s1>
</fN21>
</pA>
</standard>
<server><NO>PASCAL 01-0004051 INIST</NO>
<ET>Quality assurance in RT-PCR-based BCR/ABL diagnostics: results of an interlaboratory test and a standardization approach</ET>
<AU>BURMEISTER (T.); MAURER (J.); AIVADO (M.); ELMAAGACLI (A. H.); GRÜNEBACH (F.); HELD (K. R.); HESS (G.); HOCHHAUS (A.); HÖPPNER (W.); LENTES (K. U.); LÜBBERT (M.); SCHÄFER (K. L.); SCHAFHAUSEN (P.); SCHMIDT (C. A.); SCHÜLER (F.); SEEGER (K.); SEELIG (R.); THIEDE (C.); VIEHMANN (S.); WEBER (C.); WILHELM (S.); CHRISTMANN (A.); CLEMENT (J. H.); EBENER (U.); ENCZMANN (J.); LEO (R.); SCHLEUNING (M.); SCHOCH (R.); THIEL (E.)</AU>
<AF>Freie Universitat Berlin, Med Klinik III/Allemagne (1 aut., 2 aut., 29 aut.); Universität Düsseldorf, Klinik fur Hamatologie und Onkologie/Allemagne (3 aut.); Universität-Gesamthochschule Essen, Klinik für Knochenmarktransplantation/Allemagne (4 aut.); Universität Tübingen, Med, Klinik/Allemagne (5 aut.); Praxis Held/Kesser/Arndt Hamburg/Allemagne (6 aut.); Universität Mainz, III, Med, Klinik/Allemagne (7 aut.); Universität Heidelberg, III, Med, Klinik, Klinikum Mannheim/Allemagne (8 aut.); GLE medicon GmbH/Hamburg/Allemagne (9 aut.); Universität Trier, Abt, Molekulare Neurogenetik/Allemagne (10 aut.); Universität Freiburg, Med. Klinik I/Allemagne (11 aut.); Universitat Münster, Institut fur Pathologie/Allemagne (12 aut.); Universität Hamburg, Med. Klinik III/Allemagne (13 aut.); <sup>113</sup>
Humboldt-Universitat Berlin, Med. Klinik, Hamatologie/Onkologie/Allemagne (14 aut.); Universität Greifswald, Klinik fur Innere Medizin C/Allemagne (15 aut.); Humboldt-Universitat Berlin, Zentrum fur Kinderheilkunde/Allemagne (16 aut.); Labor Prof Seelig und Partner/Karlsruhe/Allemagne (17 aut.); Technische Universitat Dresden, Med, Klinik 1/Allemagne (18 aut.); Universität Giessen, Kinderklinik, Onkogenetisches Labor/Allemagne (19 aut.); Universität Giessen, Abt, for Hamatologie/Allemagne (20 aut.); Universität Rostock, Abt für Hämatologie/Onkologie/Allemagne (21 aut.); Universität Homburg, Institut für Humangenetik/Allemagne (22 aut.); Universität Jena, Innere Medizin II/Allemagne (23 aut.); Universität Frankfurt, Zentrum für Kinderheilkunde/Allemagne (24 aut.); Universität Düsseldorf, Knochenmarkspenderzentrale/Allemagne (25 aut.); Universität Münster, Med, Klinik A/Allemagne (26 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Leukemia; ISSN 0887-6924; Coden LEUKED; Royaume-Uni; Da. 2000; Vol. 14; No. 10; Pp. 1850-1856; Bibl. 19 ref.</SO>
<LA>Anglais</LA>
<EA>Here we describe the results of an interlaboratory test for RT-PCR-based BCR/ABL analysis. The test was organized in two parts. The number of participating laboratories in the first and second part was 27 and 20, respectively. In the first part samples containing various concentrations of plasmids with the ela2, b2a2 or b3a2 BCR/ABL transcripts were analyzed by PCR. In the second part of the test, cell samples containing various concentrations of BCR/ABL-positive cells were analyzed by RT-PCR. Overall PCR sensitivity was sufficient in approximately 90% of the tests, but a significant number of false positive results were obtained. There were significant differences in sensitivity in the cell-based analysis between the various participants. The results are discussed, and proposals are made regarding the choice of primers, controls, conditions for RNA extraction and reverse transcription.</EA>
<CC>002B24O10</CC>
<FD>Translocation chromosomique; Chromosome C9 anormal; Chromosome G22 anormal; Gène hybride; Technique; Diagnostic; Assurance qualité; Expression génique; Réaction chaîne polymérase; RNA-directed DNA polymerase; Normalisation; Homme; Etude multicentrique; Gène bcr; Gène abl</FD>
<FG>Chromosome anormal; Aberration chromosomique; Nucleotidyltransferases; Transferases; Enzyme; Biologie moléculaire</FG>
<ED>Chromosome translocation; Abnormal chromosome C9; Abnormal chromosome G22; Hybrid gene; Technique; Diagnosis; Quality assurance; Gene expression; Polymerase chain reaction; RNA-directed DNA polymerase; Standardization; Human; Multicenter study</ED>
<EG>Abnormal chromosome; Chromosomal aberration; Nucleotidyltransferases; Transferases; Enzyme; Molecular biology</EG>
<SD>Translocación cromosómica; Cromosoma C9 anormal; Cromosoma G22 anormal; Gen híbrido; Técnica; Diagnóstico; Aseguración calidad; Expresión genética; Reacción cadena polimerasa; RNA-directed DNA polymerase; Normalización; Hombre; Estudio multicéntrico</SD>
<LO>INIST-21129.354000092220510160</LO>
<ID>01-0004051</ID>
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Quality assurance in RT-PCR-based BCR/ABL diagnostics: results of an interlaboratory test and a standardization approach

Quality assurance in RT-PCR-based BCR/ABL diagnostics: results of an interlaboratory test and a standardization approach

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