Identification of trypsin‐like activity in sturgeon spermatozoa
Identifieur interne : 001B26 ( Main/Exploration ); précédent : 001B25; suivant : 001B27Identification of trypsin‐like activity in sturgeon spermatozoa
Auteurs : Andrzej Ciereszko [États-Unis, Pologne] ; Konrad Dabrowski [États-Unis] ; Feng Lin [États-Unis] ; Serge I. Doroshov [États-Unis]Source :
- Journal of Experimental Zoology [ 0022-104X ] ; 1994-05-01.
Abstract
We documented amidase activity in lake sturgeon (Acipenser fulvescens) and white sturgeon (Acipenser transmontanus) spermatozoa. This activity was inhibited by benzamidine and soybean trypsin inhibitor (SBTI), and N‐α‐p‐tosyl‐L‐lysine chloromethyl ketone‐HCl (TLCK), but not by N‐tosyl‐L‐phenylalanine chloromethyl keton (TPCK), indicating the presence of trypsin‐like activity in sturgeon spermatozoa. Higher activity occurred at pH 8.5 and 9.0 than at 8.0. No effect of 10 mM KCl and 100 mM NaCl on trypsin‐like activity was found. Calcium ions inhibited the activity at 10 and 50 mM. Similar activities were documented in neat semen and washed sperm suspension. Presence of trypsin‐like activity corresponds to the presence of acrosomes and acrosome reaction in spermatozoa of these fishes. © 1994 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jez.1402680609
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000363
- to stream Istex, to step Curation: 000362
- to stream Istex, to step Checkpoint: 001083
- to stream Main, to step Merge: 001C49
- to stream Main, to step Curation: 001B26
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of trypsin‐like activity in sturgeon spermatozoa</title>
<author><name sortKey="Ciereszko, Andrzej" sort="Ciereszko, Andrzej" uniqKey="Ciereszko A" first="Andrzej" last="Ciereszko">Andrzej Ciereszko</name>
</author>
<author><name sortKey="Dabrowski, Konrad" sort="Dabrowski, Konrad" uniqKey="Dabrowski K" first="Konrad" last="Dabrowski">Konrad Dabrowski</name>
</author>
<author><name sortKey="Lin, Feng" sort="Lin, Feng" uniqKey="Lin F" first="Feng" last="Lin">Feng Lin</name>
</author>
<author><name sortKey="Doroshov, Serge I" sort="Doroshov, Serge I" uniqKey="Doroshov S" first="Serge I." last="Doroshov">Serge I. Doroshov</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:F30F442DC6EF86E4269824B058BBEF2FF708B321</idno>
<date when="1994" year="1994">1994</date>
<idno type="doi">10.1002/jez.1402680609</idno>
<idno type="url">https://api.istex.fr/document/F30F442DC6EF86E4269824B058BBEF2FF708B321/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000363</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000363</idno>
<idno type="wicri:Area/Istex/Curation">000362</idno>
<idno type="wicri:Area/Istex/Checkpoint">001083</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001083</idno>
<idno type="wicri:doubleKey">0022-104X:1994:Ciereszko A:identification:of:trypsin</idno>
<idno type="wicri:Area/Main/Merge">001C49</idno>
<idno type="wicri:Area/Main/Curation">001B26</idno>
<idno type="wicri:Area/Main/Exploration">001B26</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Identification of trypsin‐like activity in sturgeon spermatozoa</title>
<author><name sortKey="Ciereszko, Andrzej" sort="Ciereszko, Andrzej" uniqKey="Ciereszko A" first="Andrzej" last="Ciereszko">Andrzej Ciereszko</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>School of Natural Resources, The Ohio State University, Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Pologne</country>
<wicri:regionArea>Centre for Agrotechnology and Veterinary Sciences, Polish Academy of Sciences, 10‐718 Olsztyn</wicri:regionArea>
<wicri:noRegion>10‐718 Olsztyn</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dabrowski, Konrad" sort="Dabrowski, Konrad" uniqKey="Dabrowski K" first="Konrad" last="Dabrowski">Konrad Dabrowski</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>School of Natural Resources, The Ohio State University, Columbus</wicri:cityArea>
</affiliation>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>The Ohio State University, School of Natural Resources, 210 Kottman Hall, 2021 Coffey Rd., Columbus</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Lin, Feng" sort="Lin, Feng" uniqKey="Lin F" first="Feng" last="Lin">Feng Lin</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Ohio</region>
</placeName>
<wicri:cityArea>School of Natural Resources, The Ohio State University, Columbus</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Doroshov, Serge I" sort="Doroshov, Serge I" uniqKey="Doroshov S" first="Serge I." last="Doroshov">Serge I. Doroshov</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Department of Animal Sciences, University of California, Davis</wicri:cityArea>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Journal of Experimental Zoology</title>
<title level="j" type="abbrev">J. Exp. Zool.</title>
<idno type="ISSN">0022-104X</idno>
<idno type="eISSN">1097-010X</idno>
<imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher>
<pubPlace>New York</pubPlace>
<date type="published" when="1994-05-01">1994-05-01</date>
<biblScope unit="volume">268</biblScope>
<biblScope unit="issue">6</biblScope>
<biblScope unit="page" from="486">486</biblScope>
<biblScope unit="page" to="491">491</biblScope>
</imprint>
<idno type="ISSN">0022-104X</idno>
</series>
<idno type="istex">F30F442DC6EF86E4269824B058BBEF2FF708B321</idno>
<idno type="DOI">10.1002/jez.1402680609</idno>
<idno type="ArticleID">JEZ1402680609</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0022-104X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We documented amidase activity in lake sturgeon (Acipenser fulvescens) and white sturgeon (Acipenser transmontanus) spermatozoa. This activity was inhibited by benzamidine and soybean trypsin inhibitor (SBTI), and N‐α‐p‐tosyl‐L‐lysine chloromethyl ketone‐HCl (TLCK), but not by N‐tosyl‐L‐phenylalanine chloromethyl keton (TPCK), indicating the presence of trypsin‐like activity in sturgeon spermatozoa. Higher activity occurred at pH 8.5 and 9.0 than at 8.0. No effect of 10 mM KCl and 100 mM NaCl on trypsin‐like activity was found. Calcium ions inhibited the activity at 10 and 50 mM. Similar activities were documented in neat semen and washed sperm suspension. Presence of trypsin‐like activity corresponds to the presence of acrosomes and acrosome reaction in spermatozoa of these fishes. © 1994 Wiley‐Liss, Inc.</div>
</front>
</TEI>
<affiliations><list><country><li>Pologne</li>
<li>États-Unis</li>
</country>
<region><li>Californie</li>
<li>Ohio</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Ohio"><name sortKey="Ciereszko, Andrzej" sort="Ciereszko, Andrzej" uniqKey="Ciereszko A" first="Andrzej" last="Ciereszko">Andrzej Ciereszko</name>
</region>
<name sortKey="Dabrowski, Konrad" sort="Dabrowski, Konrad" uniqKey="Dabrowski K" first="Konrad" last="Dabrowski">Konrad Dabrowski</name>
<name sortKey="Dabrowski, Konrad" sort="Dabrowski, Konrad" uniqKey="Dabrowski K" first="Konrad" last="Dabrowski">Konrad Dabrowski</name>
<name sortKey="Doroshov, Serge I" sort="Doroshov, Serge I" uniqKey="Doroshov S" first="Serge I." last="Doroshov">Serge I. Doroshov</name>
<name sortKey="Lin, Feng" sort="Lin, Feng" uniqKey="Lin F" first="Feng" last="Lin">Feng Lin</name>
</country>
<country name="Pologne"><noRegion><name sortKey="Ciereszko, Andrzej" sort="Ciereszko, Andrzej" uniqKey="Ciereszko A" first="Andrzej" last="Ciereszko">Andrzej Ciereszko</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001B26 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001B26 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:F30F442DC6EF86E4269824B058BBEF2FF708B321 |texte= Identification of trypsin‐like activity in sturgeon spermatozoa }}
![]() | This area was generated with Dilib version V0.6.27. | ![]() |