Identification of trypsin‐like activity in sturgeon spermatozoa
Identifieur interne : 000363 ( Istex/Corpus ); précédent : 000362; suivant : 000364Identification of trypsin‐like activity in sturgeon spermatozoa
Auteurs : Andrzej Ciereszko ; Konrad Dabrowski ; Feng Lin ; Serge I. DoroshovSource :
- Journal of Experimental Zoology [ 0022-104X ] ; 1994-05-01.
Abstract
We documented amidase activity in lake sturgeon (Acipenser fulvescens) and white sturgeon (Acipenser transmontanus) spermatozoa. This activity was inhibited by benzamidine and soybean trypsin inhibitor (SBTI), and N‐α‐p‐tosyl‐L‐lysine chloromethyl ketone‐HCl (TLCK), but not by N‐tosyl‐L‐phenylalanine chloromethyl keton (TPCK), indicating the presence of trypsin‐like activity in sturgeon spermatozoa. Higher activity occurred at pH 8.5 and 9.0 than at 8.0. No effect of 10 mM KCl and 100 mM NaCl on trypsin‐like activity was found. Calcium ions inhibited the activity at 10 and 50 mM. Similar activities were documented in neat semen and washed sperm suspension. Presence of trypsin‐like activity corresponds to the presence of acrosomes and acrosome reaction in spermatozoa of these fishes. © 1994 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jez.1402680609
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Zool.</title><idno type="ISSN">0022-104X</idno><idno type="eISSN">1097-010X</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>New York</pubPlace><date type="published" when="1994-05-01">1994-05-01</date><biblScope unit="volume">268</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="486">486</biblScope><biblScope unit="page" to="491">491</biblScope></imprint><idno type="ISSN">0022-104X</idno></series><idno type="istex">F30F442DC6EF86E4269824B058BBEF2FF708B321</idno><idno type="DOI">10.1002/jez.1402680609</idno><idno type="ArticleID">JEZ1402680609</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-104X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We documented amidase activity in lake sturgeon (Acipenser fulvescens) and white sturgeon (Acipenser transmontanus) spermatozoa. This activity was inhibited by benzamidine and soybean trypsin inhibitor (SBTI), and N‐α‐p‐tosyl‐L‐lysine chloromethyl ketone‐HCl (TLCK), but not by N‐tosyl‐L‐phenylalanine chloromethyl keton (TPCK), indicating the presence of trypsin‐like activity in sturgeon spermatozoa. Higher activity occurred at pH 8.5 and 9.0 than at 8.0. No effect of 10 mM KCl and 100 mM NaCl on trypsin‐like activity was found. Calcium ions inhibited the activity at 10 and 50 mM. Similar activities were documented in neat semen and washed sperm suspension. Presence of trypsin‐like activity corresponds to the presence of acrosomes and acrosome reaction in spermatozoa of these fishes. © 1994 Wiley‐Liss, Inc.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Andrzej Ciereszko</name><affiliations><json:string>School of Natural Resources, The Ohio State University, Columbus, Ohio 43210</json:string><json:string>Centre for Agrotechnology and Veterinary Sciences, Polish Academy of Sciences, 10‐718 Olsztyn, Poland</json:string></affiliations></json:item><json:item><name>Konrad Dabrowski</name><affiliations><json:string>School of Natural Resources, The Ohio State University, Columbus, Ohio 43210</json:string><json:string>The Ohio State University, School of Natural Resources, 210 Kottman Hall, 2021 Coffey Rd., Columbus, OH 43210===</json:string></affiliations></json:item><json:item><name>Feng Lin</name><affiliations><json:string>School of Natural Resources, The Ohio State University, Columbus, Ohio 43210</json:string></affiliations></json:item><json:item><name>Serge I. 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Similar activities were documented in neat semen and washed sperm suspension. 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This activity was inhibited by benzamidine and soybean trypsin inhibitor (SBTI), and N‐α‐p‐tosyl‐L‐lysine chloromethyl ketone‐HCl (TLCK), but not by N‐tosyl‐L‐phenylalanine chloromethyl keton (TPCK), indicating the presence of trypsin‐like activity in sturgeon spermatozoa. Higher activity occurred at pH 8.5 and 9.0 than at 8.0. No effect of 10 mM KCl and 100 mM NaCl on trypsin‐like activity was found. Calcium ions inhibited the activity at 10 and 50 mM. Similar activities were documented in neat semen and washed sperm suspension. Presence of trypsin‐like activity corresponds to the presence of acrosomes and acrosome reaction in spermatozoa of these fishes. © 1994 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Identification of trypsin‐like activity in sturgeon spermatozoa</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>PROTEINASE OF STURGEON SPERMATOZOA</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Identification of trypsin‐like activity in sturgeon spermatozoa</title></titleInfo><name type="personal"><namePart type="given">Andrzej</namePart><namePart type="family">Ciereszko</namePart><affiliation>School of Natural Resources, The Ohio State University, Columbus, Ohio 43210</affiliation><affiliation>Centre for Agrotechnology and Veterinary Sciences, Polish Academy of Sciences, 10‐718 Olsztyn, Poland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Konrad</namePart><namePart type="family">Dabrowski</namePart><affiliation>School of Natural Resources, The Ohio State University, Columbus, Ohio 43210</affiliation><affiliation>The Ohio State University, School of Natural Resources, 210 Kottman Hall, 2021 Coffey Rd., Columbus, OH 43210===</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Feng</namePart><namePart type="family">Lin</namePart><affiliation>School of Natural Resources, The Ohio State University, Columbus, Ohio 43210</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Serge I.</namePart><namePart type="family">Doroshov</namePart><affiliation>Department of Animal Sciences, University of California, Davis, California 95616</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="shortCommunication"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">New York</placeTerm></place><dateIssued encoding="w3cdtf">1994-05-01</dateIssued><dateCaptured encoding="w3cdtf">1993-10-12</dateCaptured><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">2</extent><extent unit="tables">2</extent><extent unit="references">24</extent></physicalDescription><abstract lang="en">We documented amidase activity in lake sturgeon (Acipenser fulvescens) and white sturgeon (Acipenser transmontanus) spermatozoa. This activity was inhibited by benzamidine and soybean trypsin inhibitor (SBTI), and N‐α‐p‐tosyl‐L‐lysine chloromethyl ketone‐HCl (TLCK), but not by N‐tosyl‐L‐phenylalanine chloromethyl keton (TPCK), indicating the presence of trypsin‐like activity in sturgeon spermatozoa. Higher activity occurred at pH 8.5 and 9.0 than at 8.0. No effect of 10 mM KCl and 100 mM NaCl on trypsin‐like activity was found. Calcium ions inhibited the activity at 10 and 50 mM. Similar activities were documented in neat semen and washed sperm suspension. Presence of trypsin‐like activity corresponds to the presence of acrosomes and acrosome reaction in spermatozoa of these fishes. © 1994 Wiley‐Liss, Inc.</abstract><relatedItem type="host"><titleInfo><title>Journal of Experimental Zoology</title></titleInfo><titleInfo type="abbreviated"><title>J. Exp. 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