Identification of trypsin‐like activity in sturgeon spermatozoa
Identifieur interne : 001B26 ( Main/Curation ); précédent : 001B25; suivant : 001B27Identification of trypsin‐like activity in sturgeon spermatozoa
Auteurs : Andrzej Ciereszko [États-Unis, Pologne] ; Konrad Dabrowski [États-Unis] ; Feng Lin [États-Unis] ; Serge I. Doroshov [États-Unis]Source :
- Journal of Experimental Zoology [ 0022-104X ] ; 1994-05-01.
Abstract
We documented amidase activity in lake sturgeon (Acipenser fulvescens) and white sturgeon (Acipenser transmontanus) spermatozoa. This activity was inhibited by benzamidine and soybean trypsin inhibitor (SBTI), and N‐α‐p‐tosyl‐L‐lysine chloromethyl ketone‐HCl (TLCK), but not by N‐tosyl‐L‐phenylalanine chloromethyl keton (TPCK), indicating the presence of trypsin‐like activity in sturgeon spermatozoa. Higher activity occurred at pH 8.5 and 9.0 than at 8.0. No effect of 10 mM KCl and 100 mM NaCl on trypsin‐like activity was found. Calcium ions inhibited the activity at 10 and 50 mM. Similar activities were documented in neat semen and washed sperm suspension. Presence of trypsin‐like activity corresponds to the presence of acrosomes and acrosome reaction in spermatozoa of these fishes. © 1994 Wiley‐Liss, Inc.
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DOI: 10.1002/jez.1402680609
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Zool.</title><idno type="ISSN">0022-104X</idno><idno type="eISSN">1097-010X</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>New York</pubPlace><date type="published" when="1994-05-01">1994-05-01</date><biblScope unit="volume">268</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="486">486</biblScope><biblScope unit="page" to="491">491</biblScope></imprint><idno type="ISSN">0022-104X</idno></series><idno type="istex">F30F442DC6EF86E4269824B058BBEF2FF708B321</idno><idno type="DOI">10.1002/jez.1402680609</idno><idno type="ArticleID">JEZ1402680609</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-104X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We documented amidase activity in lake sturgeon (Acipenser fulvescens) and white sturgeon (Acipenser transmontanus) spermatozoa. This activity was inhibited by benzamidine and soybean trypsin inhibitor (SBTI), and N‐α‐p‐tosyl‐L‐lysine chloromethyl ketone‐HCl (TLCK), but not by N‐tosyl‐L‐phenylalanine chloromethyl keton (TPCK), indicating the presence of trypsin‐like activity in sturgeon spermatozoa. Higher activity occurred at pH 8.5 and 9.0 than at 8.0. No effect of 10 mM KCl and 100 mM NaCl on trypsin‐like activity was found. Calcium ions inhibited the activity at 10 and 50 mM. Similar activities were documented in neat semen and washed sperm suspension. Presence of trypsin‐like activity corresponds to the presence of acrosomes and acrosome reaction in spermatozoa of these fishes. © 1994 Wiley‐Liss, Inc.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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