Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay
Identifieur interne : 001F25 ( Main/Exploration ); précédent : 001F24; suivant : 001F26Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay
Auteurs : H. J. Th. Goos [Pays-Bas] ; R. De Leeuw [Pays-Bas] ; E. Burzawa-Gérard [France] ; M. Terlou [Pays-Bas] ; C. J. J. Richter [Pays-Bas]Source :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1986.
Abstract
Glycoprotein gonadotropic hormone (GTH) was purified from 2000 pituitaries of male and female African catfish, Clarias gariepinus. The first step was chromatography on concanavalin A-Sepharose followed by filtration on Ultrogel Aca 54, chromatography on DEAE-cellulose, and filtration on Ultrogel Aca 54, respectively. Finally, the purified fractions were analyzed by polyacrylamide gel electrophoresis. The gonadotropic activity in the different fractions was characterized using two tests: the radioimmunoassay for carp gonadotropin-β subunit was used to quantify the immunoreactive GTH and a cAMP accumulation test was applied to measure the GTH biological activity. The purified glycoprotein GTH was used to raise antibodies and to develop a radioimmunoassay. This resulted in an assay with a variation between assays of approximately 4%, a precision of 4–8%, and an accuracy of 4–8%. GTH levels can be measured over a range of 0.8 to 12.5 ng/ml.
Url:
DOI: 10.1016/0016-6480(86)90152-8
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000869
- to stream Istex, to step Curation: 000867
- to stream Istex, to step Checkpoint: 001327
- to stream Main, to step Merge: 002099
- to stream Main, to step Curation: 001F25
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay</title>
<author><name sortKey="Goos, H J Th" sort="Goos, H J Th" uniqKey="Goos H" first="H. J. Th." last="Goos">H. J. Th. Goos</name>
</author>
<author><name sortKey="De Leeuw, R" sort="De Leeuw, R" uniqKey="De Leeuw R" first="R." last="De Leeuw">R. De Leeuw</name>
</author>
<author><name sortKey="Burzawa Gerard, E" sort="Burzawa Gerard, E" uniqKey="Burzawa Gerard E" first="E." last="Burzawa-Gérard">E. Burzawa-Gérard</name>
</author>
<author><name sortKey="Terlou, M" sort="Terlou, M" uniqKey="Terlou M" first="M." last="Terlou">M. Terlou</name>
</author>
<author><name sortKey="Richter, C J J" sort="Richter, C J J" uniqKey="Richter C" first="C. J. J." last="Richter">C. J. J. Richter</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:954E16E678C382666E9A2A5EE517EC0A4C74133D</idno>
<date when="1986" year="1986">1986</date>
<idno type="doi">10.1016/0016-6480(86)90152-8</idno>
<idno type="url">https://api.istex.fr/document/954E16E678C382666E9A2A5EE517EC0A4C74133D/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000869</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000869</idno>
<idno type="wicri:Area/Istex/Curation">000867</idno>
<idno type="wicri:Area/Istex/Checkpoint">001327</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001327</idno>
<idno type="wicri:doubleKey">0016-6480:1986:Goos H:purification:of:gonadotropic</idno>
<idno type="wicri:Area/Main/Merge">002099</idno>
<idno type="wicri:Area/Main/Curation">001F25</idno>
<idno type="wicri:Area/Main/Exploration">001F25</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay</title>
<author><name sortKey="Goos, H J Th" sort="Goos, H J Th" uniqKey="Goos H" first="H. J. Th." last="Goos">H. J. Th. Goos</name>
<affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht</wicri:regionArea>
<wicri:noRegion>University of Utrecht</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="De Leeuw, R" sort="De Leeuw, R" uniqKey="De Leeuw R" first="R." last="De Leeuw">R. De Leeuw</name>
<affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht</wicri:regionArea>
<wicri:noRegion>University of Utrecht</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Burzawa Gerard, E" sort="Burzawa Gerard, E" uniqKey="Burzawa Gerard E" first="E." last="Burzawa-Gérard">E. Burzawa-Gérard</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Laboratoire de Physiologie Générale et Comparée, Paris</wicri:regionArea>
<placeName><region type="region">Île-de-France</region>
<region type="old region">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Terlou, M" sort="Terlou, M" uniqKey="Terlou M" first="M." last="Terlou">M. Terlou</name>
<affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht</wicri:regionArea>
<wicri:noRegion>University of Utrecht</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Richter, C J J" sort="Richter, C J J" uniqKey="Richter C" first="C. J. J." last="Richter">C. J. J. Richter</name>
<affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Fish Culture and Inland Fisheries, Agricultural University, Wageningen</wicri:regionArea>
<wicri:noRegion>Wageningen</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">General and Comparative Endocrinology</title>
<title level="j" type="abbrev">YGCEN</title>
<idno type="ISSN">0016-6480</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1986">1986</date>
<biblScope unit="volume">63</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="162">162</biblScope>
<biblScope unit="page" to="170">170</biblScope>
</imprint>
<idno type="ISSN">0016-6480</idno>
</series>
<idno type="istex">954E16E678C382666E9A2A5EE517EC0A4C74133D</idno>
<idno type="DOI">10.1016/0016-6480(86)90152-8</idno>
<idno type="PII">0016-6480(86)90152-8</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0016-6480</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Glycoprotein gonadotropic hormone (GTH) was purified from 2000 pituitaries of male and female African catfish, Clarias gariepinus. The first step was chromatography on concanavalin A-Sepharose followed by filtration on Ultrogel Aca 54, chromatography on DEAE-cellulose, and filtration on Ultrogel Aca 54, respectively. Finally, the purified fractions were analyzed by polyacrylamide gel electrophoresis. The gonadotropic activity in the different fractions was characterized using two tests: the radioimmunoassay for carp gonadotropin-β subunit was used to quantify the immunoreactive GTH and a cAMP accumulation test was applied to measure the GTH biological activity. The purified glycoprotein GTH was used to raise antibodies and to develop a radioimmunoassay. This resulted in an assay with a variation between assays of approximately 4%, a precision of 4–8%, and an accuracy of 4–8%. GTH levels can be measured over a range of 0.8 to 12.5 ng/ml.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
<li>Pays-Bas</li>
</country>
<region><li>Île-de-France</li>
</region>
<settlement><li>Paris</li>
</settlement>
</list>
<tree><country name="Pays-Bas"><noRegion><name sortKey="Goos, H J Th" sort="Goos, H J Th" uniqKey="Goos H" first="H. J. Th." last="Goos">H. J. Th. Goos</name>
</noRegion>
<name sortKey="De Leeuw, R" sort="De Leeuw, R" uniqKey="De Leeuw R" first="R." last="De Leeuw">R. De Leeuw</name>
<name sortKey="Richter, C J J" sort="Richter, C J J" uniqKey="Richter C" first="C. J. J." last="Richter">C. J. J. Richter</name>
<name sortKey="Terlou, M" sort="Terlou, M" uniqKey="Terlou M" first="M." last="Terlou">M. Terlou</name>
</country>
<country name="France"><region name="Île-de-France"><name sortKey="Burzawa Gerard, E" sort="Burzawa Gerard, E" uniqKey="Burzawa Gerard E" first="E." last="Burzawa-Gérard">E. Burzawa-Gérard</name>
</region>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001F25 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001F25 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:954E16E678C382666E9A2A5EE517EC0A4C74133D |texte= Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay }}
![]() | This area was generated with Dilib version V0.6.27. | ![]() |