Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay
Identifieur interne : 000867 ( Istex/Curation ); précédent : 000866; suivant : 000868Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay
Auteurs : H. J. Th. Goos [Pays-Bas] ; R. De Leeuw [Pays-Bas] ; E. Burzawa-Gérard [France] ; M. Terlou [Pays-Bas] ; C. J. J. Richter [Pays-Bas]Source :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1986.
Abstract
Glycoprotein gonadotropic hormone (GTH) was purified from 2000 pituitaries of male and female African catfish, Clarias gariepinus. The first step was chromatography on concanavalin A-Sepharose followed by filtration on Ultrogel Aca 54, chromatography on DEAE-cellulose, and filtration on Ultrogel Aca 54, respectively. Finally, the purified fractions were analyzed by polyacrylamide gel electrophoresis. The gonadotropic activity in the different fractions was characterized using two tests: the radioimmunoassay for carp gonadotropin-β subunit was used to quantify the immunoreactive GTH and a cAMP accumulation test was applied to measure the GTH biological activity. The purified glycoprotein GTH was used to raise antibodies and to develop a radioimmunoassay. This resulted in an assay with a variation between assays of approximately 4%, a precision of 4–8%, and an accuracy of 4–8%. GTH levels can be measured over a range of 0.8 to 12.5 ng/ml.
Url:
DOI: 10.1016/0016-6480(86)90152-8
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000869
Links to Exploration step
ISTEX:954E16E678C382666E9A2A5EE517EC0A4C74133DLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay</title><author><name sortKey="Goos, H J Th" sort="Goos, H J Th" uniqKey="Goos H" first="H. J. Th." last="Goos">H. J. Th. Goos</name><affiliation wicri:level="1"><mods:affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</mods:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht</wicri:regionArea></affiliation></author><author><name sortKey="De Leeuw, R" sort="De Leeuw, R" uniqKey="De Leeuw R" first="R." last="De Leeuw">R. De Leeuw</name><affiliation wicri:level="1"><mods:affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</mods:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht</wicri:regionArea></affiliation></author><author><name sortKey="Burzawa Gerard, E" sort="Burzawa Gerard, E" uniqKey="Burzawa Gerard E" first="E." last="Burzawa-Gérard">E. Burzawa-Gérard</name><affiliation wicri:level="1"><mods:affiliation>Laboratoire de Physiologie Générale et Comparée, Paris, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Physiologie Générale et Comparée, Paris</wicri:regionArea></affiliation></author><author><name sortKey="Terlou, M" sort="Terlou, M" uniqKey="Terlou M" first="M." last="Terlou">M. Terlou</name><affiliation wicri:level="1"><mods:affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</mods:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht</wicri:regionArea></affiliation></author><author><name sortKey="Richter, C J J" sort="Richter, C J J" uniqKey="Richter C" first="C. J. J." last="Richter">C. J. J. Richter</name><affiliation wicri:level="1"><mods:affiliation>Department of Fish Culture and Inland Fisheries, Agricultural University, Wageningen, The Netherlands</mods:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Fish Culture and Inland Fisheries, Agricultural University, Wageningen</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:954E16E678C382666E9A2A5EE517EC0A4C74133D</idno><date when="1986" year="1986">1986</date><idno type="doi">10.1016/0016-6480(86)90152-8</idno><idno type="url">https://api.istex.fr/document/954E16E678C382666E9A2A5EE517EC0A4C74133D/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000869</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000869</idno><idno type="wicri:Area/Istex/Curation">000867</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay</title><author><name sortKey="Goos, H J Th" sort="Goos, H J Th" uniqKey="Goos H" first="H. J. Th." last="Goos">H. J. Th. Goos</name><affiliation wicri:level="1"><mods:affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</mods:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht</wicri:regionArea></affiliation></author><author><name sortKey="De Leeuw, R" sort="De Leeuw, R" uniqKey="De Leeuw R" first="R." last="De Leeuw">R. De Leeuw</name><affiliation wicri:level="1"><mods:affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</mods:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht</wicri:regionArea></affiliation></author><author><name sortKey="Burzawa Gerard, E" sort="Burzawa Gerard, E" uniqKey="Burzawa Gerard E" first="E." last="Burzawa-Gérard">E. Burzawa-Gérard</name><affiliation wicri:level="1"><mods:affiliation>Laboratoire de Physiologie Générale et Comparée, Paris, France</mods:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Physiologie Générale et Comparée, Paris</wicri:regionArea></affiliation></author><author><name sortKey="Terlou, M" sort="Terlou, M" uniqKey="Terlou M" first="M." last="Terlou">M. Terlou</name><affiliation wicri:level="1"><mods:affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</mods:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht</wicri:regionArea></affiliation></author><author><name sortKey="Richter, C J J" sort="Richter, C J J" uniqKey="Richter C" first="C. J. J." last="Richter">C. J. J. Richter</name><affiliation wicri:level="1"><mods:affiliation>Department of Fish Culture and Inland Fisheries, Agricultural University, Wageningen, The Netherlands</mods:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Fish Culture and Inland Fisheries, Agricultural University, Wageningen</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">General and Comparative Endocrinology</title><title level="j" type="abbrev">YGCEN</title><idno type="ISSN">0016-6480</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1986">1986</date><biblScope unit="volume">63</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="162">162</biblScope><biblScope unit="page" to="170">170</biblScope></imprint><idno type="ISSN">0016-6480</idno></series><idno type="istex">954E16E678C382666E9A2A5EE517EC0A4C74133D</idno><idno type="DOI">10.1016/0016-6480(86)90152-8</idno><idno type="PII">0016-6480(86)90152-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0016-6480</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Glycoprotein gonadotropic hormone (GTH) was purified from 2000 pituitaries of male and female African catfish, Clarias gariepinus. The first step was chromatography on concanavalin A-Sepharose followed by filtration on Ultrogel Aca 54, chromatography on DEAE-cellulose, and filtration on Ultrogel Aca 54, respectively. Finally, the purified fractions were analyzed by polyacrylamide gel electrophoresis. The gonadotropic activity in the different fractions was characterized using two tests: the radioimmunoassay for carp gonadotropin-β subunit was used to quantify the immunoreactive GTH and a cAMP accumulation test was applied to measure the GTH biological activity. The purified glycoprotein GTH was used to raise antibodies and to develop a radioimmunoassay. This resulted in an assay with a variation between assays of approximately 4%, a precision of 4–8%, and an accuracy of 4–8%. GTH levels can be measured over a range of 0.8 to 12.5 ng/ml.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000867 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000867 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:954E16E678C382666E9A2A5EE517EC0A4C74133D
|texte= Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay
}}
| This area was generated with Dilib version V0.6.27. |  |