Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay
Identifieur interne : 000869 ( Istex/Corpus ); précédent : 000868; suivant : 000870Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay
Auteurs : H. J. Th. Goos ; R. De Leeuw ; E. Burzawa-Gérard ; M. Terlou ; C. J. J. RichterSource :
- General and Comparative Endocrinology [ 0016-6480 ] ; 1986.
Abstract
Glycoprotein gonadotropic hormone (GTH) was purified from 2000 pituitaries of male and female African catfish, Clarias gariepinus. The first step was chromatography on concanavalin A-Sepharose followed by filtration on Ultrogel Aca 54, chromatography on DEAE-cellulose, and filtration on Ultrogel Aca 54, respectively. Finally, the purified fractions were analyzed by polyacrylamide gel electrophoresis. The gonadotropic activity in the different fractions was characterized using two tests: the radioimmunoassay for carp gonadotropin-β subunit was used to quantify the immunoreactive GTH and a cAMP accumulation test was applied to measure the GTH biological activity. The purified glycoprotein GTH was used to raise antibodies and to develop a radioimmunoassay. This resulted in an assay with a variation between assays of approximately 4%, a precision of 4–8%, and an accuracy of 4–8%. GTH levels can be measured over a range of 0.8 to 12.5 ng/ml.
Url:
DOI: 10.1016/0016-6480(86)90152-8
Links to Exploration step
ISTEX:954E16E678C382666E9A2A5EE517EC0A4C74133DLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay</title>
<author><name sortKey="Goos, H J Th" sort="Goos, H J Th" uniqKey="Goos H" first="H. J. Th." last="Goos">H. J. Th. Goos</name>
<affiliation><mods:affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="De Leeuw, R" sort="De Leeuw, R" uniqKey="De Leeuw R" first="R." last="De Leeuw">R. De Leeuw</name>
<affiliation><mods:affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Burzawa Gerard, E" sort="Burzawa Gerard, E" uniqKey="Burzawa Gerard E" first="E." last="Burzawa-Gérard">E. Burzawa-Gérard</name>
<affiliation><mods:affiliation>Laboratoire de Physiologie Générale et Comparée, Paris, France</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Terlou, M" sort="Terlou, M" uniqKey="Terlou M" first="M." last="Terlou">M. Terlou</name>
<affiliation><mods:affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Richter, C J J" sort="Richter, C J J" uniqKey="Richter C" first="C. J. J." last="Richter">C. J. J. Richter</name>
<affiliation><mods:affiliation>Department of Fish Culture and Inland Fisheries, Agricultural University, Wageningen, The Netherlands</mods:affiliation>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:954E16E678C382666E9A2A5EE517EC0A4C74133D</idno>
<date when="1986" year="1986">1986</date>
<idno type="doi">10.1016/0016-6480(86)90152-8</idno>
<idno type="url">https://api.istex.fr/document/954E16E678C382666E9A2A5EE517EC0A4C74133D/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000869</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000869</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay</title>
<author><name sortKey="Goos, H J Th" sort="Goos, H J Th" uniqKey="Goos H" first="H. J. Th." last="Goos">H. J. Th. Goos</name>
<affiliation><mods:affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="De Leeuw, R" sort="De Leeuw, R" uniqKey="De Leeuw R" first="R." last="De Leeuw">R. De Leeuw</name>
<affiliation><mods:affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Burzawa Gerard, E" sort="Burzawa Gerard, E" uniqKey="Burzawa Gerard E" first="E." last="Burzawa-Gérard">E. Burzawa-Gérard</name>
<affiliation><mods:affiliation>Laboratoire de Physiologie Générale et Comparée, Paris, France</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Terlou, M" sort="Terlou, M" uniqKey="Terlou M" first="M." last="Terlou">M. Terlou</name>
<affiliation><mods:affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</mods:affiliation>
</affiliation>
</author>
<author><name sortKey="Richter, C J J" sort="Richter, C J J" uniqKey="Richter C" first="C. J. J." last="Richter">C. J. J. Richter</name>
<affiliation><mods:affiliation>Department of Fish Culture and Inland Fisheries, Agricultural University, Wageningen, The Netherlands</mods:affiliation>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">General and Comparative Endocrinology</title>
<title level="j" type="abbrev">YGCEN</title>
<idno type="ISSN">0016-6480</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1986">1986</date>
<biblScope unit="volume">63</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="162">162</biblScope>
<biblScope unit="page" to="170">170</biblScope>
</imprint>
<idno type="ISSN">0016-6480</idno>
</series>
<idno type="istex">954E16E678C382666E9A2A5EE517EC0A4C74133D</idno>
<idno type="DOI">10.1016/0016-6480(86)90152-8</idno>
<idno type="PII">0016-6480(86)90152-8</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0016-6480</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Glycoprotein gonadotropic hormone (GTH) was purified from 2000 pituitaries of male and female African catfish, Clarias gariepinus. The first step was chromatography on concanavalin A-Sepharose followed by filtration on Ultrogel Aca 54, chromatography on DEAE-cellulose, and filtration on Ultrogel Aca 54, respectively. Finally, the purified fractions were analyzed by polyacrylamide gel electrophoresis. The gonadotropic activity in the different fractions was characterized using two tests: the radioimmunoassay for carp gonadotropin-β subunit was used to quantify the immunoreactive GTH and a cAMP accumulation test was applied to measure the GTH biological activity. The purified glycoprotein GTH was used to raise antibodies and to develop a radioimmunoassay. This resulted in an assay with a variation between assays of approximately 4%, a precision of 4–8%, and an accuracy of 4–8%. GTH levels can be measured over a range of 0.8 to 12.5 ng/ml.</div>
</front>
</TEI>
<istex><corpusName>elsevier</corpusName>
<author><json:item><name>H.J.Th. Goos</name>
<affiliations><json:string>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</json:string>
</affiliations>
</json:item>
<json:item><name>R. de Leeuw</name>
<affiliations><json:string>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</json:string>
</affiliations>
</json:item>
<json:item><name>E. Burzawa-Gérard</name>
<affiliations><json:string>Laboratoire de Physiologie Générale et Comparée, Paris, France</json:string>
</affiliations>
</json:item>
<json:item><name>M. Terlou</name>
<affiliations><json:string>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</json:string>
</affiliations>
</json:item>
<json:item><name>C.J.J. Richter</name>
<affiliations><json:string>Department of Fish Culture and Inland Fisheries, Agricultural University, Wageningen, The Netherlands</json:string>
</affiliations>
</json:item>
</author>
<language><json:string>eng</json:string>
</language>
<originalGenre><json:string>Full-length article</json:string>
</originalGenre>
<abstract>Glycoprotein gonadotropic hormone (GTH) was purified from 2000 pituitaries of male and female African catfish, Clarias gariepinus. The first step was chromatography on concanavalin A-Sepharose followed by filtration on Ultrogel Aca 54, chromatography on DEAE-cellulose, and filtration on Ultrogel Aca 54, respectively. Finally, the purified fractions were analyzed by polyacrylamide gel electrophoresis. The gonadotropic activity in the different fractions was characterized using two tests: the radioimmunoassay for carp gonadotropin-β subunit was used to quantify the immunoreactive GTH and a cAMP accumulation test was applied to measure the GTH biological activity. The purified glycoprotein GTH was used to raise antibodies and to develop a radioimmunoassay. This resulted in an assay with a variation between assays of approximately 4%, a precision of 4–8%, and an accuracy of 4–8%. GTH levels can be measured over a range of 0.8 to 12.5 ng/ml.</abstract>
<qualityIndicators><score>5.843</score>
<pdfVersion>1.3</pdfVersion>
<pdfPageSize>476 x 720 pts</pdfPageSize>
<refBibsNative>true</refBibsNative>
<keywordCount>0</keywordCount>
<abstractCharCount>950</abstractCharCount>
<pdfWordCount>4175</pdfWordCount>
<pdfCharCount>26731</pdfCharCount>
<pdfPageCount>9</pdfPageCount>
<abstractWordCount>139</abstractWordCount>
</qualityIndicators>
<title>Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay</title>
<pii><json:string>0016-6480(86)90152-8</json:string>
</pii>
<genre><json:string>research-article</json:string>
</genre>
<serie><volume>Vol. IX</volume>
<editor><json:item><name>W.S. Hoar</name>
</json:item>
<json:item><name>D.J. Randall</name>
</json:item>
<json:item><name>E.M. Donaldson</name>
</json:item>
</editor>
<pages><last>403</last>
<first>351</first>
</pages>
<language><json:string>unknown</json:string>
</language>
<title>Fish Physiology</title>
</serie>
<host><volume>63</volume>
<pii><json:string>S0016-6480(00)X0270-5</json:string>
</pii>
<pages><last>170</last>
<first>162</first>
</pages>
<issn><json:string>0016-6480</json:string>
</issn>
<issue>2</issue>
<genre><json:string>journal</json:string>
</genre>
<language><json:string>unknown</json:string>
</language>
<title>General and Comparative Endocrinology</title>
<publicationDate>1986</publicationDate>
</host>
<categories><wos><json:string>science</json:string>
<json:string>endocrinology & metabolism</json:string>
</wos>
<scienceMetrix><json:string>health sciences</json:string>
<json:string>clinical medicine</json:string>
<json:string>endocrinology & metabolism</json:string>
</scienceMetrix>
</categories>
<publicationDate>1986</publicationDate>
<copyrightDate>1986</copyrightDate>
<doi><json:string>10.1016/0016-6480(86)90152-8</json:string>
</doi>
<id>954E16E678C382666E9A2A5EE517EC0A4C74133D</id>
<score>0.05329554</score>
<fulltext><json:item><extension>pdf</extension>
<original>true</original>
<mimetype>application/pdf</mimetype>
<uri>https://api.istex.fr/document/954E16E678C382666E9A2A5EE517EC0A4C74133D/fulltext/pdf</uri>
</json:item>
<json:item><extension>zip</extension>
<original>false</original>
<mimetype>application/zip</mimetype>
<uri>https://api.istex.fr/document/954E16E678C382666E9A2A5EE517EC0A4C74133D/fulltext/zip</uri>
</json:item>
<istex:fulltextTEI uri="https://api.istex.fr/document/954E16E678C382666E9A2A5EE517EC0A4C74133D/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay</title>
</titleStmt>
<publicationStmt><authority>ISTEX</authority>
<publisher>ELSEVIER</publisher>
<availability><p>ELSEVIER</p>
</availability>
<date>1986</date>
</publicationStmt>
<sourceDesc><biblStruct type="inbook"><analytic><title level="a">Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay</title>
<author xml:id="author-1"><persName><forename type="first">H.J.Th.</forename>
<surname>Goos</surname>
</persName>
<affiliation>To whom correspondence should be addressed: Zoological Laboratory, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands.</affiliation>
<affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</affiliation>
</author>
<author xml:id="author-2"><persName><forename type="first">R.</forename>
<surname>de Leeuw</surname>
</persName>
<affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</affiliation>
</author>
<author xml:id="author-3"><persName><forename type="first">E.</forename>
<surname>Burzawa-Gérard</surname>
</persName>
<affiliation>Laboratoire de Physiologie Générale et Comparée, Paris, France</affiliation>
</author>
<author xml:id="author-4"><persName><forename type="first">M.</forename>
<surname>Terlou</surname>
</persName>
<affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</affiliation>
</author>
<author xml:id="author-5"><persName><forename type="first">C.J.J.</forename>
<surname>Richter</surname>
</persName>
<affiliation>Department of Fish Culture and Inland Fisheries, Agricultural University, Wageningen, The Netherlands</affiliation>
</author>
</analytic>
<monogr><title level="j">General and Comparative Endocrinology</title>
<title level="j" type="abbrev">YGCEN</title>
<idno type="pISSN">0016-6480</idno>
<idno type="PII">S0016-6480(00)X0270-5</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1986"></date>
<biblScope unit="volume">63</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="162">162</biblScope>
<biblScope unit="page" to="170">170</biblScope>
</imprint>
</monogr>
<idno type="istex">954E16E678C382666E9A2A5EE517EC0A4C74133D</idno>
<idno type="DOI">10.1016/0016-6480(86)90152-8</idno>
<idno type="PII">0016-6480(86)90152-8</idno>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><creation><date>1986</date>
</creation>
<langUsage><language ident="en">en</language>
</langUsage>
<abstract xml:lang="en"><p>Glycoprotein gonadotropic hormone (GTH) was purified from 2000 pituitaries of male and female African catfish, Clarias gariepinus. The first step was chromatography on concanavalin A-Sepharose followed by filtration on Ultrogel Aca 54, chromatography on DEAE-cellulose, and filtration on Ultrogel Aca 54, respectively. Finally, the purified fractions were analyzed by polyacrylamide gel electrophoresis. The gonadotropic activity in the different fractions was characterized using two tests: the radioimmunoassay for carp gonadotropin-β subunit was used to quantify the immunoreactive GTH and a cAMP accumulation test was applied to measure the GTH biological activity. The purified glycoprotein GTH was used to raise antibodies and to develop a radioimmunoassay. This resulted in an assay with a variation between assays of approximately 4%, a precision of 4–8%, and an accuracy of 4–8%. GTH levels can be measured over a range of 0.8 to 12.5 ng/ml.</p>
</abstract>
</profileDesc>
<revisionDesc><change when="1986">Published</change>
</revisionDesc>
</teiHeader>
</istex:fulltextTEI>
<json:item><extension>txt</extension>
<original>false</original>
<mimetype>text/plain</mimetype>
<uri>https://api.istex.fr/document/954E16E678C382666E9A2A5EE517EC0A4C74133D/fulltext/txt</uri>
</json:item>
</fulltext>
<metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration>
<istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType>
<istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YGCEN</jid>
<aid>86901528</aid>
<ce:pii>0016-6480(86)90152-8</ce:pii>
<ce:doi>10.1016/0016-6480(86)90152-8</ce:doi>
<ce:copyright type="unknown" year="1986"></ce:copyright>
</item-info>
<head><ce:title>Purification of gonadotropic hormone from the pituitary of the African catfish, <ce:italic>Clarias gariepinus</ce:italic>
(Burchell), and the development of a homologous radioimmunoassay</ce:title>
<ce:author-group><ce:author><ce:given-name>H.J.Th.</ce:given-name>
<ce:surname>Goos</ce:surname>
<ce:cross-ref refid="COR1"><ce:sup>1</ce:sup>
</ce:cross-ref>
<ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>R.</ce:given-name>
<ce:surname>de Leeuw</ce:surname>
<ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>E.</ce:given-name>
<ce:surname>Burzawa-Gérard</ce:surname>
<ce:cross-ref refid="AFF2"><ce:sup>∗</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>M.</ce:given-name>
<ce:surname>Terlou</ce:surname>
<ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:author><ce:given-name>C.J.J.</ce:given-name>
<ce:surname>Richter</ce:surname>
<ce:cross-ref refid="AFF3"><ce:sup>†</ce:sup>
</ce:cross-ref>
</ce:author>
<ce:affiliation id="AFF1"><ce:label>a</ce:label>
<ce:textfn>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</ce:textfn>
</ce:affiliation>
<ce:affiliation id="AFF2"><ce:label>b</ce:label>
<ce:textfn>Laboratoire de Physiologie Générale et Comparée, Paris, France</ce:textfn>
</ce:affiliation>
<ce:affiliation id="AFF3"><ce:label>c</ce:label>
<ce:textfn>Department of Fish Culture and Inland Fisheries, Agricultural University, Wageningen, The Netherlands</ce:textfn>
</ce:affiliation>
<ce:correspondence id="COR1"><ce:label>1</ce:label>
<ce:text>To whom correspondence should be addressed: Zoological Laboratory, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands.</ce:text>
</ce:correspondence>
</ce:author-group>
<ce:date-accepted day="11" month="12" year="1985"></ce:date-accepted>
<ce:abstract><ce:section-title>Abstract</ce:section-title>
<ce:abstract-sec><ce:simple-para>Glycoprotein gonadotropic hormone (GTH) was purified from 2000 pituitaries of male and female African catfish, <ce:italic>Clarias gariepinus</ce:italic>
. The first step was chromatography on concanavalin A-Sepharose followed by filtration on Ultrogel Aca 54, chromatography on DEAE-cellulose, and filtration on Ultrogel Aca 54, respectively. Finally, the purified fractions were analyzed by polyacrylamide gel electrophoresis. The gonadotropic activity in the different fractions was characterized using two tests: the radioimmunoassay for carp gonadotropin-β subunit was used to quantify the immunoreactive GTH and a cAMP accumulation test was applied to measure the GTH biological activity. The purified glycoprotein GTH was used to raise antibodies and to develop a radioimmunoassay. This resulted in an assay with a variation between assays of approximately 4%, a precision of 4–8%, and an accuracy of 4–8%. GTH levels can be measured over a range of 0.8 to 12.5 ng/ml.</ce:simple-para>
</ce:abstract-sec>
</ce:abstract>
</head>
</converted-article>
</istex:document>
</istex:metadataXml>
<mods version="3.6"><titleInfo><title>Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay</title>
</titleInfo>
<titleInfo type="alternative" contentType="CDATA"><title>Purification of gonadotropic hormone from the pituitary of the African catfish,</title>
</titleInfo>
<name type="personal"><namePart type="given">H.J.Th.</namePart>
<namePart type="family">Goos</namePart>
<affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</affiliation>
<description>To whom correspondence should be addressed: Zoological Laboratory, University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands.</description>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">R.</namePart>
<namePart type="family">de Leeuw</namePart>
<affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">E.</namePart>
<namePart type="family">Burzawa-Gérard</namePart>
<affiliation>Laboratoire de Physiologie Générale et Comparée, Paris, France</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">M.</namePart>
<namePart type="family">Terlou</namePart>
<affiliation>Zoological Laboratory, Research Group for Comparative Endocrinology, University of Utrecht, The Netherlands</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">C.J.J.</namePart>
<namePart type="family">Richter</namePart>
<affiliation>Department of Fish Culture and Inland Fisheries, Agricultural University, Wageningen, The Netherlands</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<typeOfResource>text</typeOfResource>
<genre type="research-article" displayLabel="Full-length article"></genre>
<originInfo><publisher>ELSEVIER</publisher>
<dateIssued encoding="w3cdtf">1986</dateIssued>
<copyrightDate encoding="w3cdtf">1986</copyrightDate>
</originInfo>
<language><languageTerm type="code" authority="iso639-2b">eng</languageTerm>
<languageTerm type="code" authority="rfc3066">en</languageTerm>
</language>
<physicalDescription><internetMediaType>text/html</internetMediaType>
</physicalDescription>
<abstract lang="en">Glycoprotein gonadotropic hormone (GTH) was purified from 2000 pituitaries of male and female African catfish, Clarias gariepinus. The first step was chromatography on concanavalin A-Sepharose followed by filtration on Ultrogel Aca 54, chromatography on DEAE-cellulose, and filtration on Ultrogel Aca 54, respectively. Finally, the purified fractions were analyzed by polyacrylamide gel electrophoresis. The gonadotropic activity in the different fractions was characterized using two tests: the radioimmunoassay for carp gonadotropin-β subunit was used to quantify the immunoreactive GTH and a cAMP accumulation test was applied to measure the GTH biological activity. The purified glycoprotein GTH was used to raise antibodies and to develop a radioimmunoassay. This resulted in an assay with a variation between assays of approximately 4%, a precision of 4–8%, and an accuracy of 4–8%. GTH levels can be measured over a range of 0.8 to 12.5 ng/ml.</abstract>
<relatedItem type="host"><titleInfo><title>General and Comparative Endocrinology</title>
</titleInfo>
<titleInfo type="abbreviated"><title>YGCEN</title>
</titleInfo>
<genre type="journal">journal</genre>
<originInfo><dateIssued encoding="w3cdtf">198608</dateIssued>
</originInfo>
<identifier type="ISSN">0016-6480</identifier>
<identifier type="PII">S0016-6480(00)X0270-5</identifier>
<part><date>198608</date>
<detail type="volume"><number>63</number>
<caption>vol.</caption>
</detail>
<detail type="issue"><number>2</number>
<caption>no.</caption>
</detail>
<extent unit="issue pages"><start>157</start>
<end>319</end>
</extent>
<extent unit="pages"><start>162</start>
<end>170</end>
</extent>
</part>
</relatedItem>
<identifier type="istex">954E16E678C382666E9A2A5EE517EC0A4C74133D</identifier>
<identifier type="DOI">10.1016/0016-6480(86)90152-8</identifier>
<identifier type="PII">0016-6480(86)90152-8</identifier>
<recordInfo><recordContentSource>ELSEVIER</recordContentSource>
</recordInfo>
</mods>
</metadata>
</istex>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000869 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000869 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Istex |étape= Corpus |type= RBID |clé= ISTEX:954E16E678C382666E9A2A5EE517EC0A4C74133D |texte= Purification of gonadotropic hormone from the pituitary of the African catfish, Clarias gariepinus (Burchell), and the development of a homologous radioimmunoassay }}
![]() | This area was generated with Dilib version V0.6.27. | ![]() |