Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females
Identifieur interne : 001A44 ( Main/Curation ); précédent : 001A43; suivant : 001A45Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females
Auteurs : B. Cuisset [France] ; A. Fostier [France] ; Patrick Williot [France] ; C. Bennetau-Pelissero [France] ; Françoise Le Menn [France]Source :
- Fish Physiology and Biochemistry [ 0920-1742 ] ; 1995-08-01.
Abstract
Abstract: High levels of 11-ketotestosterone (11KT) were found (49 to 160 ng ml−1) in plasma of Siberian sturgeon females during the end of their reproductive cycle. These levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by UV absorption after HPLC (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). In order to find the origin of 11KT synthesis, ovaries were incubated (30 min and 2h at 20°C) with tritiated 17-hydroxyprogesterone (17OHP) or with tritiated androstenedione (A4). Testosterone (conversion rate from tritiated 17OHP: 4%) and 11-ketotestosterone (conversion rate from tritiated A4: 1.6%) were identified as metabolites of respectively 17OHP and A4 (TLC, HPLC and crystallization). 11β-hydroxyandrostenedione (11βOHA4) and 11β-hydroxytestosterone (11βOHT) were suggested to be intermediate metabolites. Besides interrenal and blood cells were incubated respectively with tritiated cortisol and tritiated A4. 11βOHA4 was identified in interrenal incubation (yield from tritiated cortisol: 1.2%). 11KT in interrenal (yield from tritiated cortisol: 0.14%), and 11βOHA4 and 11KT in blood cells (yield from tritiated A4: 1.6%), were suspected to be synthesized (TLC, HPLC, acetylation). No significant metabolization of tritiated cortisol could be found in liver. The possible contribution of each of these tissues to high 11KT levels found in plasma is discussed.
Url:
DOI: 10.1007/BF00004069
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000D05
- to stream Istex, to step Curation: Pour aller vers cette notice dans l'étape Curation :000D03
- to stream Istex, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001012
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000697
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :000697
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000697
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000515
- to stream Ncbi, to step Curation: Pour aller vers cette notice dans l'étape Curation :000515
- to stream Ncbi, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000515
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :001B60
Links to Exploration step
ISTEX:89AA26D114C8C03C08AFCAFA40A8CB705CBF6094Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females</title>
<author><name sortKey="Cuisset, B" sort="Cuisset, B" uniqKey="Cuisset B" first="B." last="Cuisset">B. Cuisset</name>
</author>
<author><name sortKey="Fostier, A" sort="Fostier, A" uniqKey="Fostier A" first="A." last="Fostier">A. Fostier</name>
</author>
<author><name sortKey="Williot, P" sort="Williot, P" uniqKey="Williot P" first="P." last="Williot">Patrick Williot</name>
<affiliation><country>France</country>
<placeName><settlement type="city">Bordeaux</settlement>
<settlement type="town">Cestas</settlement>
<region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
</placeName>
<orgName type="laboratoire" n="5">Cemagref - centre de Bordeaux</orgName>
<orgName type="institution">Centre national du machinisme agricole, du génie rural, des eaux et des forêts</orgName>
<orgName type="institution">Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture</orgName>
</affiliation>
</author>
<author><name sortKey="Bennetau Pelissero, C" sort="Bennetau Pelissero, C" uniqKey="Bennetau Pelissero C" first="C." last="Bennetau-Pelissero">C. Bennetau-Pelissero</name>
</author>
<author><name sortKey="Le Menn, F" sort="Le Menn, F" uniqKey="Le Menn F" first="F." last="Le Menn">Françoise Le Menn</name>
<affiliation><country>France</country>
<placeName><settlement type="city">Bordeaux</settlement>
<region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
</placeName>
<orgName type="university" n="3">Université Bordeaux I</orgName>
<orgName type="institution" wicri:auto="newGroup">Université de Bordeaux</orgName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:89AA26D114C8C03C08AFCAFA40A8CB705CBF6094</idno>
<date when="1995" year="1995">1995</date>
<idno type="doi">10.1007/BF00004069</idno>
<idno type="url">https://api.istex.fr/document/89AA26D114C8C03C08AFCAFA40A8CB705CBF6094/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000D05</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000D05</idno>
<idno type="wicri:Area/Istex/Curation">000D03</idno>
<idno type="wicri:Area/Istex/Checkpoint">001012</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001012</idno>
<idno type="wicri:doubleKey">0920-1742:1995:Cuisset B:occurrence:and:in</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:24197499</idno>
<idno type="wicri:Area/PubMed/Corpus">000697</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000697</idno>
<idno type="wicri:Area/PubMed/Curation">000697</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000697</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000697</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000697</idno>
<idno type="wicri:Area/Ncbi/Merge">000515</idno>
<idno type="wicri:Area/Ncbi/Curation">000515</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000515</idno>
<idno type="wicri:doubleKey">0920-1742:1995:Cuisset B:occurrence:and:in</idno>
<idno type="wicri:Area/Main/Merge">001B60</idno>
<idno type="wicri:Area/Main/Curation">001A44</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females</title>
<author><name sortKey="Cuisset, B" sort="Cuisset, B" uniqKey="Cuisset B" first="B." last="Cuisset">B. Cuisset</name>
<affiliation wicri:level="4"><country xml:lang="fr">France</country>
<wicri:regionArea>Université Bordeaux I, U.A.-I.N.R.A. de Biologie de la Reproduction des Poissons, Avenue des Facultés, 33405, Talence Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
<settlement type="city">Talence</settlement>
</placeName>
<orgName type="university">Université Bordeaux I</orgName>
</affiliation>
</author>
<author><name sortKey="Fostier, A" sort="Fostier, A" uniqKey="Fostier A" first="A." last="Fostier">A. Fostier</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut National de la Recherche Agronomique, Laboratoire de Physiologie des Poissons, Campus de Beaulieu, 35042, Rennes Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Région Bretagne</region>
<settlement type="city">Rennes</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Williot, P" sort="Williot, P" uniqKey="Williot P" first="P." last="Williot">Patrick Williot</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Division Aquaculture et Pechê, Centre National du Machinisme Agricole, du Génie Rural, des Eaux des Forêts, 50 Avenue de Verdun, BP 3, 33611, Gazinet Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
<settlement type="city">Gazinet</settlement>
</placeName>
<placeName><settlement type="city">Bordeaux</settlement>
<settlement type="town">Cestas</settlement>
<region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
</placeName>
<orgName type="laboratoire" n="5">Cemagref - centre de Bordeaux</orgName>
<orgName type="institution">Centre national du machinisme agricole, du génie rural, des eaux et des forêts</orgName>
<orgName type="institution">Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture</orgName>
</affiliation>
</author>
<author><name sortKey="Bennetau Pelissero, C" sort="Bennetau Pelissero, C" uniqKey="Bennetau Pelissero C" first="C." last="Bennetau-Pelissero">C. Bennetau-Pelissero</name>
<affiliation wicri:level="4"><country xml:lang="fr">France</country>
<wicri:regionArea>Université Bordeaux I, U.A.-I.N.R.A. de Biologie de la Reproduction des Poissons, Avenue des Facultés, 33405, Talence Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
<settlement type="city">Talence</settlement>
</placeName>
<orgName type="university">Université Bordeaux I</orgName>
</affiliation>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>ENITA Bordeaux, 33170, Gradigham</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
<settlement type="city">Gradigham</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Le Menn, F" sort="Le Menn, F" uniqKey="Le Menn F" first="F." last="Le Menn">Françoise Le Menn</name>
<affiliation wicri:level="4"><country xml:lang="fr">France</country>
<wicri:regionArea>Université Bordeaux I, U.A.-I.N.R.A. de Biologie de la Reproduction des Poissons, Avenue des Facultés, 33405, Talence Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
<settlement type="city">Talence</settlement>
</placeName>
<orgName type="university">Université Bordeaux I</orgName>
<placeName><settlement type="city">Bordeaux</settlement>
<region type="region" nuts="2">Nouvelle-Aquitaine</region>
<region type="old region" nuts="2">Aquitaine</region>
</placeName>
<orgName type="university" n="3">Université Bordeaux I</orgName>
<orgName type="institution" wicri:auto="newGroup">Université de Bordeaux</orgName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Fish Physiology and Biochemistry</title>
<title level="j" type="abbrev">Fish Physiol Biochem</title>
<idno type="ISSN">0920-1742</idno>
<idno type="eISSN">1573-5168</idno>
<imprint><publisher>Kluwer Academic Publishers</publisher>
<pubPlace>Dordrecht</pubPlace>
<date type="published" when="1995-08-01">1995-08-01</date>
<biblScope unit="volume">14</biblScope>
<biblScope unit="issue">4</biblScope>
<biblScope unit="page" from="313">313</biblScope>
<biblScope unit="page" to="322">322</biblScope>
</imprint>
<idno type="ISSN">0920-1742</idno>
</series>
<idno type="istex">89AA26D114C8C03C08AFCAFA40A8CB705CBF6094</idno>
<idno type="DOI">10.1007/BF00004069</idno>
<idno type="ArticleID">BF00004069</idno>
<idno type="ArticleID">Art6</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0920-1742</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: High levels of 11-ketotestosterone (11KT) were found (49 to 160 ng ml−1) in plasma of Siberian sturgeon females during the end of their reproductive cycle. These levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by UV absorption after HPLC (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). In order to find the origin of 11KT synthesis, ovaries were incubated (30 min and 2h at 20°C) with tritiated 17-hydroxyprogesterone (17OHP) or with tritiated androstenedione (A4). Testosterone (conversion rate from tritiated 17OHP: 4%) and 11-ketotestosterone (conversion rate from tritiated A4: 1.6%) were identified as metabolites of respectively 17OHP and A4 (TLC, HPLC and crystallization). 11β-hydroxyandrostenedione (11βOHA4) and 11β-hydroxytestosterone (11βOHT) were suggested to be intermediate metabolites. Besides interrenal and blood cells were incubated respectively with tritiated cortisol and tritiated A4. 11βOHA4 was identified in interrenal incubation (yield from tritiated cortisol: 1.2%). 11KT in interrenal (yield from tritiated cortisol: 0.14%), and 11βOHA4 and 11KT in blood cells (yield from tritiated A4: 1.6%), were suspected to be synthesized (TLC, HPLC, acetylation). No significant metabolization of tritiated cortisol could be found in liver. The possible contribution of each of these tissues to high 11KT levels found in plasma is discussed.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001A44 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 001A44 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= Main |étape= Curation |type= RBID |clé= ISTEX:89AA26D114C8C03C08AFCAFA40A8CB705CBF6094 |texte= Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females }}
This area was generated with Dilib version V0.6.27. |