Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females
Identifieur interne : 000D05 ( Istex/Corpus ); précédent : 000D04; suivant : 000D06Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females
Auteurs : B. Cuisset ; A. Fostier ; P. Williot ; C. Bennetau-Pelissero ; F. Le MennSource :
- Fish Physiology and Biochemistry [ 0920-1742 ] ; 1995-08-01.
Abstract
Abstract: High levels of 11-ketotestosterone (11KT) were found (49 to 160 ng ml−1) in plasma of Siberian sturgeon females during the end of their reproductive cycle. These levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by UV absorption after HPLC (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). In order to find the origin of 11KT synthesis, ovaries were incubated (30 min and 2h at 20°C) with tritiated 17-hydroxyprogesterone (17OHP) or with tritiated androstenedione (A4). Testosterone (conversion rate from tritiated 17OHP: 4%) and 11-ketotestosterone (conversion rate from tritiated A4: 1.6%) were identified as metabolites of respectively 17OHP and A4 (TLC, HPLC and crystallization). 11β-hydroxyandrostenedione (11βOHA4) and 11β-hydroxytestosterone (11βOHT) were suggested to be intermediate metabolites. Besides interrenal and blood cells were incubated respectively with tritiated cortisol and tritiated A4. 11βOHA4 was identified in interrenal incubation (yield from tritiated cortisol: 1.2%). 11KT in interrenal (yield from tritiated cortisol: 0.14%), and 11βOHA4 and 11KT in blood cells (yield from tritiated A4: 1.6%), were suspected to be synthesized (TLC, HPLC, acetylation). No significant metabolization of tritiated cortisol could be found in liver. The possible contribution of each of these tissues to high 11KT levels found in plasma is discussed.
Url:
DOI: 10.1007/BF00004069
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<front><div type="abstract" xml:lang="en">Abstract: High levels of 11-ketotestosterone (11KT) were found (49 to 160 ng ml−1) in plasma of Siberian sturgeon females during the end of their reproductive cycle. These levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by UV absorption after HPLC (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). In order to find the origin of 11KT synthesis, ovaries were incubated (30 min and 2h at 20°C) with tritiated 17-hydroxyprogesterone (17OHP) or with tritiated androstenedione (A4). Testosterone (conversion rate from tritiated 17OHP: 4%) and 11-ketotestosterone (conversion rate from tritiated A4: 1.6%) were identified as metabolites of respectively 17OHP and A4 (TLC, HPLC and crystallization). 11β-hydroxyandrostenedione (11βOHA4) and 11β-hydroxytestosterone (11βOHT) were suggested to be intermediate metabolites. Besides interrenal and blood cells were incubated respectively with tritiated cortisol and tritiated A4. 11βOHA4 was identified in interrenal incubation (yield from tritiated cortisol: 1.2%). 11KT in interrenal (yield from tritiated cortisol: 0.14%), and 11βOHA4 and 11KT in blood cells (yield from tritiated A4: 1.6%), were suspected to be synthesized (TLC, HPLC, acetylation). No significant metabolization of tritiated cortisol could be found in liver. The possible contribution of each of these tissues to high 11KT levels found in plasma is discussed.</div>
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<abstract>Abstract: High levels of 11-ketotestosterone (11KT) were found (49 to 160 ng ml−1) in plasma of Siberian sturgeon females during the end of their reproductive cycle. These levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by UV absorption after HPLC (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). In order to find the origin of 11KT synthesis, ovaries were incubated (30 min and 2h at 20°C) with tritiated 17-hydroxyprogesterone (17OHP) or with tritiated androstenedione (A4). Testosterone (conversion rate from tritiated 17OHP: 4%) and 11-ketotestosterone (conversion rate from tritiated A4: 1.6%) were identified as metabolites of respectively 17OHP and A4 (TLC, HPLC and crystallization). 11β-hydroxyandrostenedione (11βOHA4) and 11β-hydroxytestosterone (11βOHT) were suggested to be intermediate metabolites. Besides interrenal and blood cells were incubated respectively with tritiated cortisol and tritiated A4. 11βOHA4 was identified in interrenal incubation (yield from tritiated cortisol: 1.2%). 11KT in interrenal (yield from tritiated cortisol: 0.14%), and 11βOHA4 and 11KT in blood cells (yield from tritiated A4: 1.6%), were suspected to be synthesized (TLC, HPLC, acetylation). No significant metabolization of tritiated cortisol could be found in liver. The possible contribution of each of these tissues to high 11KT levels found in plasma is discussed.</abstract>
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<abstract xml:lang="en"><p>Abstract: High levels of 11-ketotestosterone (11KT) were found (49 to 160 ng ml−1) in plasma of Siberian sturgeon females during the end of their reproductive cycle. These levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by UV absorption after HPLC (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). In order to find the origin of 11KT synthesis, ovaries were incubated (30 min and 2h at 20°C) with tritiated 17-hydroxyprogesterone (17OHP) or with tritiated androstenedione (A4). Testosterone (conversion rate from tritiated 17OHP: 4%) and 11-ketotestosterone (conversion rate from tritiated A4: 1.6%) were identified as metabolites of respectively 17OHP and A4 (TLC, HPLC and crystallization). 11β-hydroxyandrostenedione (11βOHA4) and 11β-hydroxytestosterone (11βOHT) were suggested to be intermediate metabolites. Besides interrenal and blood cells were incubated respectively with tritiated cortisol and tritiated A4. 11βOHA4 was identified in interrenal incubation (yield from tritiated cortisol: 1.2%). 11KT in interrenal (yield from tritiated cortisol: 0.14%), and 11βOHA4 and 11KT in blood cells (yield from tritiated A4: 1.6%), were suspected to be synthesized (TLC, HPLC, acetylation). No significant metabolization of tritiated cortisol could be found in liver. The possible contribution of each of these tissues to high 11KT levels found in plasma is discussed.</p>
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<VolumeIDStart>14</VolumeIDStart>
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<IssueIDStart>4</IssueIDStart>
<IssueIDEnd>4</IssueIDEnd>
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<ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>B.</GivenName>
<FamilyName>Cuisset</FamilyName>
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<Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>A.</GivenName>
<FamilyName>Fostier</FamilyName>
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<Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>P.</GivenName>
<FamilyName>Williot</FamilyName>
</AuthorName>
</Author>
<Author AffiliationIDS="Aff1 Aff4"><AuthorName DisplayOrder="Western"><GivenName>C.</GivenName>
<FamilyName>Bennetau-Pelissero</FamilyName>
</AuthorName>
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<Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>F.</GivenName>
<Particle>Le</Particle>
<FamilyName>Menn</FamilyName>
</AuthorName>
</Author>
<Affiliation ID="Aff1"><OrgName>Université Bordeaux I, U.A.-I.N.R.A. de Biologie de la Reproduction des Poissons</OrgName>
<OrgAddress><Street>Avenue des Facultés</Street>
<Postcode>33405</Postcode>
<City>Talence Cedex</City>
<Country>France</Country>
</OrgAddress>
</Affiliation>
<Affiliation ID="Aff2"><OrgName>Institut National de la Recherche Agronomique, Laboratoire de Physiologie des Poissons, Campus de Beaulieu</OrgName>
<OrgAddress><Postcode>35042</Postcode>
<City>Rennes Cedex</City>
<Country>France</Country>
</OrgAddress>
</Affiliation>
<Affiliation ID="Aff3"><OrgDivision>Division Aquaculture et Pechê</OrgDivision>
<OrgName>Centre National du Machinisme Agricole, du Génie Rural, des Eaux des Forêts</OrgName>
<OrgAddress><Street>50 Avenue de Verdun</Street>
<Postcode>BP 3, 33611</Postcode>
<City>Gazinet Cedex</City>
<Country>France</Country>
</OrgAddress>
</Affiliation>
<Affiliation ID="Aff4"><OrgName>ENITA Bordeaux</OrgName>
<OrgAddress><Postcode>33170</Postcode>
<City>Gradigham</City>
<Country>France</Country>
</OrgAddress>
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<Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading>
<Para>High levels of 11-ketotestosterone (11KT) were found (49 to 160 ng ml<Superscript>−1</Superscript>
) in plasma of Siberian sturgeon females during the end of their reproductive cycle. These levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by UV absorption after HPLC (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). In order to find the origin of 11KT synthesis, ovaries were incubated (30 min and 2h at 20°C) with tritiated 17-hydroxyprogesterone (17OHP) or with tritiated androstenedione (A4). Testosterone (conversion rate from tritiated 17OHP: 4%) and 11-ketotestosterone (conversion rate from tritiated A4: 1.6%) were identified as metabolites of respectively 17OHP and A4 (TLC, HPLC and crystallization). 11β-hydroxyandrostenedione (11βOHA4) and 11β-hydroxytestosterone (11βOHT) were suggested to be intermediate metabolites. Besides interrenal and blood cells were incubated respectively with tritiated cortisol and tritiated A4. 11βOHA4 was identified in interrenal incubation (yield from tritiated cortisol: 1.2%). 11KT in interrenal (yield from tritiated cortisol: 0.14%), and 11βOHA4 and 11KT in blood cells (yield from tritiated A4: 1.6%), were suspected to be synthesized (TLC, HPLC, acetylation). No significant metabolization of tritiated cortisol could be found in liver. The possible contribution of each of these tissues to high 11KT levels found in plasma is discussed.</Para>
</Abstract>
<KeywordGroup Language="En"><Heading>Keywords</Heading>
<Keyword>11KT</Keyword>
<Keyword>steroidogenesis</Keyword>
<Keyword>plasma steroids</Keyword>
<Keyword>ovary</Keyword>
<Keyword>sturgeon</Keyword>
<Keyword><Emphasis Type="Italic">Acipenser baeri</Emphasis>
</Keyword>
</KeywordGroup>
<ArticleNote Type="Misc"><SimplePara>To whom correspondence should be sent</SimplePara>
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<mods version="3.6"><titleInfo lang="en"><title>Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females</title>
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<titleInfo type="alternative" contentType="CDATA" lang="en"><title>Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females</title>
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<name type="personal"><namePart type="given">B.</namePart>
<namePart type="family">Cuisset</namePart>
<affiliation>Université Bordeaux I, U.A.-I.N.R.A. de Biologie de la Reproduction des Poissons, Avenue des Facultés, 33405, Talence Cedex, France</affiliation>
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<affiliation>Institut National de la Recherche Agronomique, Laboratoire de Physiologie des Poissons, Campus de Beaulieu, 35042, Rennes Cedex, France</affiliation>
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<name type="personal"><namePart type="given">P.</namePart>
<namePart type="family">Williot</namePart>
<affiliation>Division Aquaculture et Pechê, Centre National du Machinisme Agricole, du Génie Rural, des Eaux des Forêts, 50 Avenue de Verdun, BP 3, 33611, Gazinet Cedex, France</affiliation>
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<name type="personal"><namePart type="given">C.</namePart>
<namePart type="family">Bennetau-Pelissero</namePart>
<affiliation>Université Bordeaux I, U.A.-I.N.R.A. de Biologie de la Reproduction des Poissons, Avenue des Facultés, 33405, Talence Cedex, France</affiliation>
<affiliation>ENITA Bordeaux, 33170, Gradigham, France</affiliation>
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<name type="personal"><namePart type="given">F.</namePart>
<namePart type="family">Le Menn</namePart>
<affiliation>Université Bordeaux I, U.A.-I.N.R.A. de Biologie de la Reproduction des Poissons, Avenue des Facultés, 33405, Talence Cedex, France</affiliation>
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<dateIssued encoding="w3cdtf">1995-08-01</dateIssued>
<copyrightDate encoding="w3cdtf">1995</copyrightDate>
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<abstract lang="en">Abstract: High levels of 11-ketotestosterone (11KT) were found (49 to 160 ng ml−1) in plasma of Siberian sturgeon females during the end of their reproductive cycle. These levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by UV absorption after HPLC (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). In order to find the origin of 11KT synthesis, ovaries were incubated (30 min and 2h at 20°C) with tritiated 17-hydroxyprogesterone (17OHP) or with tritiated androstenedione (A4). Testosterone (conversion rate from tritiated 17OHP: 4%) and 11-ketotestosterone (conversion rate from tritiated A4: 1.6%) were identified as metabolites of respectively 17OHP and A4 (TLC, HPLC and crystallization). 11β-hydroxyandrostenedione (11βOHA4) and 11β-hydroxytestosterone (11βOHT) were suggested to be intermediate metabolites. Besides interrenal and blood cells were incubated respectively with tritiated cortisol and tritiated A4. 11βOHA4 was identified in interrenal incubation (yield from tritiated cortisol: 1.2%). 11KT in interrenal (yield from tritiated cortisol: 0.14%), and 11βOHA4 and 11KT in blood cells (yield from tritiated A4: 1.6%), were suspected to be synthesized (TLC, HPLC, acetylation). No significant metabolization of tritiated cortisol could be found in liver. The possible contribution of each of these tissues to high 11KT levels found in plasma is discussed.</abstract>
<relatedItem type="host"><titleInfo><title>Fish Physiology and Biochemistry</title>
</titleInfo>
<titleInfo type="abbreviated"><title>Fish Physiol Biochem</title>
</titleInfo>
<genre type="journal" displayLabel="Archive Journal"></genre>
<originInfo><dateIssued encoding="w3cdtf">1995-08-01</dateIssued>
<copyrightDate encoding="w3cdtf">1995</copyrightDate>
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<subject><genre>Life Sciences</genre>
<topic>Animal Biochemistry</topic>
<topic>Hydrobiology</topic>
<topic>Zoology</topic>
<topic>Animal Anatomy / Morphology / Histology</topic>
<topic>Animal Physiology</topic>
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<identifier type="ISSN">0920-1742</identifier>
<identifier type="eISSN">1573-5168</identifier>
<identifier type="JournalID">10695</identifier>
<identifier type="IssueArticleCount">8</identifier>
<identifier type="VolumeIssueCount">6</identifier>
<part><date>1995</date>
<detail type="volume"><number>14</number>
<caption>vol.</caption>
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<detail type="issue"><number>4</number>
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<extent unit="pages"><start>313</start>
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