Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females.
Identifieur interne : 000697 ( PubMed/Curation ); précédent : 000696; suivant : 000698Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females.
Auteurs : B. Cuisset [France] ; A. Fostier ; P. Williot ; C. Bennetau-Pelissero ; F. Le MennSource :
- Fish physiology and biochemistry [ 0920-1742 ] ; 1995.
Abstract
High levels of 11-ketotestosterone (11KT) were found (49 to 160 ng ml(-1)) in plasma of Siberian sturgeon females during the end of their reproductive cycle. These levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by UV absorption after HPLC (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). In order to find the origin of 11KT synthesis, ovaries were incubated (30 min and 2h at 20°C) with tritiated 17-hydroxyprogesterone (17OHP) or with tritiated androstenedione (A4). Testosterone (conversion rate from tritiated 17OHP: 4%) and 11-ketotestosterone (conversion rate from tritiated A4: 1.6%) were identified as metabolites of respectively 17OHP and A4 (TLC, HPLC and crystallization). 11β-hydroxyandrostenedione (11βOHA4) and 11β-hydroxytestosterone (11βOHT) were suggested to be intermediate metabolites. Besides interrenal and blood cells were incubated respectively with tritiated cortisol and tritiated A4. 11βOHA4 was identified in interrenal incubation (yield from tritiated cortisol: 1.2%). 11KT in interrenal (yield from tritiated cortisol: 0.14%), and 11βOHA4 and 11KT in blood cells (yield from tritiated A4: 1.6%), were suspected to be synthesized (TLC, HPLC, acetylation). No significant metabolization of tritiated cortisol could be found in liver. The possible contribution of each of these tissues to high 11KT levels found in plasma is discussed.
DOI: 10.1007/BF00004069
PubMed: 24197499
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Cuisset</name><affiliation wicri:level="1"><nlm:affiliation>Université Bordeaux I, U.A.-I.N.R.A. de Biologie de la Reproduction des Poissons, Avenue des Facultés, 33405, Talence Cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université Bordeaux I, U.A.-I.N.R.A. de Biologie de la Reproduction des Poissons, Avenue des Facultés, 33405, Talence Cedex</wicri:regionArea></affiliation></author><author><name sortKey="Fostier, A" sort="Fostier, A" uniqKey="Fostier A" first="A" last="Fostier">A. Fostier</name></author><author><name sortKey="Williot, P" sort="Williot, P" uniqKey="Williot P" first="P" last="Williot">P. Williot</name></author><author><name sortKey="Bennetau Pelissero, C" sort="Bennetau Pelissero, C" uniqKey="Bennetau Pelissero C" first="C" last="Bennetau-Pelissero">C. Bennetau-Pelissero</name></author><author><name sortKey="Le Menn, F" sort="Le Menn, F" uniqKey="Le Menn F" first="F" last="Le Menn">F. Le Menn</name></author></analytic><series><title level="j">Fish physiology and biochemistry</title><idno type="ISSN">0920-1742</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">High levels of 11-ketotestosterone (11KT) were found (49 to 160 ng ml(-1)) in plasma of Siberian sturgeon females during the end of their reproductive cycle. These levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by UV absorption after HPLC (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). In order to find the origin of 11KT synthesis, ovaries were incubated (30 min and 2h at 20°C) with tritiated 17-hydroxyprogesterone (17OHP) or with tritiated androstenedione (A4). Testosterone (conversion rate from tritiated 17OHP: 4%) and 11-ketotestosterone (conversion rate from tritiated A4: 1.6%) were identified as metabolites of respectively 17OHP and A4 (TLC, HPLC and crystallization). 11β-hydroxyandrostenedione (11βOHA4) and 11β-hydroxytestosterone (11βOHT) were suggested to be intermediate metabolites. Besides interrenal and blood cells were incubated respectively with tritiated cortisol and tritiated A4. 11βOHA4 was identified in interrenal incubation (yield from tritiated cortisol: 1.2%). 11KT in interrenal (yield from tritiated cortisol: 0.14%), and 11βOHA4 and 11KT in blood cells (yield from tritiated A4: 1.6%), were suspected to be synthesized (TLC, HPLC, acetylation). No significant metabolization of tritiated cortisol could be found in liver. The possible contribution of each of these tissues to high 11KT levels found in plasma is discussed.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">24197499</PMID><DateCreated><Year>2013</Year><Month>11</Month><Day>07</Day></DateCreated><DateCompleted><Year>2013</Year><Month>11</Month><Day>08</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0920-1742</ISSN><JournalIssue CitedMedium="Print"><Volume>14</Volume><Issue>4</Issue><PubDate><Year>1995</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Fish physiology and biochemistry</Title><ISOAbbreviation>Fish Physiol. Biochem.</ISOAbbreviation></Journal><ArticleTitle>Occurrence and in vitro biosynthesis of 11-ketotestosterone in Siberian sturgeon, Acipenser baeri Brandt maturing females.</ArticleTitle><Pagination><MedlinePgn>313-22</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/BF00004069</ELocationID><Abstract><AbstractText>High levels of 11-ketotestosterone (11KT) were found (49 to 160 ng ml(-1)) in plasma of Siberian sturgeon females during the end of their reproductive cycle. These levels were measured either by specific radioimmunoassay, or both by specific radioimmunoassay and by UV absorption after HPLC (isocratic conditions, 33% methanol, 26% acetonitrile, 41% water). In order to find the origin of 11KT synthesis, ovaries were incubated (30 min and 2h at 20°C) with tritiated 17-hydroxyprogesterone (17OHP) or with tritiated androstenedione (A4). Testosterone (conversion rate from tritiated 17OHP: 4%) and 11-ketotestosterone (conversion rate from tritiated A4: 1.6%) were identified as metabolites of respectively 17OHP and A4 (TLC, HPLC and crystallization). 11β-hydroxyandrostenedione (11βOHA4) and 11β-hydroxytestosterone (11βOHT) were suggested to be intermediate metabolites. Besides interrenal and blood cells were incubated respectively with tritiated cortisol and tritiated A4. 11βOHA4 was identified in interrenal incubation (yield from tritiated cortisol: 1.2%). 11KT in interrenal (yield from tritiated cortisol: 0.14%), and 11βOHA4 and 11KT in blood cells (yield from tritiated A4: 1.6%), were suspected to be synthesized (TLC, HPLC, acetylation). No significant metabolization of tritiated cortisol could be found in liver. The possible contribution of each of these tissues to high 11KT levels found in plasma is discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cuisset</LastName><ForeName>B</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Université Bordeaux I, U.A.-I.N.R.A. de Biologie de la Reproduction des Poissons, Avenue des Facultés, 33405, Talence Cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fostier</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Williot</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Bennetau-Pelissero</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Le Menn</LastName><ForeName>F</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Fish Physiol Biochem</MedlineTA><NlmUniqueID>100955049</NlmUniqueID><ISSNLinking>0920-1742</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="accepted"><Year>1995</Year><Month>01</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>11</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>1995</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1995</Year><Month>8</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24197499</ArticleId><ArticleId IdType="doi">10.1007/BF00004069</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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