EsturgeonV1, Main, Curation, bibRecord, 001A19

Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti

Identifieur interne : 001A19 ( Main/Curation ); précédent : 001A18; suivant : 001A20

Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti

Auteurs : D. W. Lescheid [Canada] ; J. F. F. Powell [Canada] ; W. H. Fischer [États-Unis] ; M. Park [États-Unis] ; A. Craig [États-Unis] ; O. Bukovskaya [Russie] ; I. A. Barannikova [Russie] ; N. M. Sherwood [Canada]

Source :

Regulatory Peptides [ 0167-0115 ] ; 1995.

RBID : ISTEX:EF9B0369BC6ACADB6F0B0A548F4B69C0DEA68AEB

Descripteurs français

Pascal (Inist)
- Acipenser gueldenstaedti, Evolution biologique, Gonadotrophine RH, Purification, Séquence aminoacide.

English descriptors

KwdEn :
- Amino Acid Sequence, Aminoacid sequence, Animals, Biological evolution, Brain Chemistry, Chromatography, High Pressure Liquid, Female, Fishes, Gonadotropin RH, Gonadotropin-Releasing Hormone (analysis), Gonadotropin-Releasing Hormone (chemistry), Male, Mass Spectrometry, Molecular Sequence Data, Purification, Sequence Analysis.
MESH :
- chemical , analysis : Gonadotropin-Releasing Hormone.
- chemical , chemistry : Gonadotropin-Releasing Hormone.
- Amino Acid Sequence, Animals, Brain Chemistry, Chromatography, High Pressure Liquid, Female, Fishes, Male, Mass Spectrometry, Molecular Sequence Data, Sequence Analysis.

Abstract

The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl aminopeptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.

Url:

https://api.istex.fr/document/EF9B0369BC6ACADB6F0B0A548F4B69C0DEA68AEB/fulltext/pdf

DOI: 10.1016/0167-0115(94)00118-H

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ISTEX:EF9B0369BC6ACADB6F0B0A548F4B69C0DEA68AEB

Le document en format XML

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<front><div type="abstract" xml:lang="en">The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl aminopeptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.</div>
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<author><name sortKey="Lescheid, D W" sort="Lescheid, D W" uniqKey="Lescheid D" first="D. W." last="Lescheid">D. W. Lescheid</name>
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<author><name sortKey="Powell, J F F" sort="Powell, J F F" uniqKey="Powell J" first="J. F. F." last="Powell">J. F. F. Powell</name>
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<author><name sortKey="Fischer, W H" sort="Fischer, W H" uniqKey="Fischer W" first="W. H." last="Fischer">W. H. Fischer</name>
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<author><name sortKey="Park, M" sort="Park, M" uniqKey="Park M" first="M." last="Park">M. Park</name>
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<author><name sortKey="Craig, A" sort="Craig, A" uniqKey="Craig A" first="A." last="Craig">A. Craig</name>
</author>
<author><name sortKey="Bukovskaya, O" sort="Bukovskaya, O" uniqKey="Bukovskaya O" first="O." last="Bukovskaya">O. Bukovskaya</name>
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<author><name sortKey="Barannikova, I A" sort="Barannikova, I A" uniqKey="Barannikova I" first="I. A." last="Barannikova">I. A. Barannikova</name>
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<sourceDesc><biblStruct><analytic><title level="a">Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti</title>
<author><name sortKey="Lescheid, D W" sort="Lescheid, D W" uniqKey="Lescheid D" first="D. W." last="Lescheid">D. W. Lescheid</name>
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<author><name sortKey="Powell, J F F" sort="Powell, J F F" uniqKey="Powell J" first="J. F. F." last="Powell">J. F. F. Powell</name>
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<author><name sortKey="Fischer, W H" sort="Fischer, W H" uniqKey="Fischer W" first="W. H." last="Fischer">W. H. Fischer</name>
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<author><name sortKey="Park, M" sort="Park, M" uniqKey="Park M" first="M." last="Park">M. Park</name>
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<wicri:regionArea>The Clayton Foundation Laboratories for Peptide Biology, The Salk Institute, La Jolla, CA 92037</wicri:regionArea>
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<author><name sortKey="Craig, A" sort="Craig, A" uniqKey="Craig A" first="A." last="Craig">A. Craig</name>
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<wicri:regionArea>The Clayton Foundation Laboratories for Peptide Biology, The Salk Institute, La Jolla, CA 92037</wicri:regionArea>
<placeName><region type="state">Californie</region>
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</affiliation>
</author>
<author><name sortKey="Bukovskaya, O" sort="Bukovskaya, O" uniqKey="Bukovskaya O" first="O." last="Bukovskaya">O. Bukovskaya</name>
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<wicri:regionArea>Physiological Institute, St. Petersburg University, St. Petersburg 199034</wicri:regionArea>
<wicri:noRegion>St. Petersburg 199034</wicri:noRegion>
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<author><name sortKey="Barannikova, I A" sort="Barannikova, I A" uniqKey="Barannikova I" first="I. A." last="Barannikova">I. A. Barannikova</name>
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<wicri:regionArea>Physiological Institute, St. Petersburg University, St. Petersburg 199034</wicri:regionArea>
<wicri:noRegion>St. Petersburg 199034</wicri:noRegion>
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<author><name sortKey="Sherwood, N M" sort="Sherwood, N M" uniqKey="Sherwood N" first="N. M." last="Sherwood">N. M. Sherwood</name>
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<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
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<front><div type="abstract" xml:lang="en">The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl aminopeptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.</div>
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<author><name sortKey="Lescheid, D W" sort="Lescheid, D W" uniqKey="Lescheid D" first="D W" last="Lescheid">D W Lescheid</name>
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<author><name sortKey="Powell, J F" sort="Powell, J F" uniqKey="Powell J" first="J F" last="Powell">J F Powell</name>
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<author><name sortKey="Fischer, W H" sort="Fischer, W H" uniqKey="Fischer W" first="W H" last="Fischer">W H Fischer</name>
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<author><name sortKey="Park, M" sort="Park, M" uniqKey="Park M" first="M" last="Park">M. Park</name>
</author>
<author><name sortKey="Craig, A" sort="Craig, A" uniqKey="Craig A" first="A" last="Craig">A. Craig</name>
</author>
<author><name sortKey="Bukovskaya, O" sort="Bukovskaya, O" uniqKey="Bukovskaya O" first="O" last="Bukovskaya">O. Bukovskaya</name>
</author>
<author><name sortKey="Barannikova, I A" sort="Barannikova, I A" uniqKey="Barannikova I" first="I A" last="Barannikova">I A Barannikova</name>
</author>
<author><name sortKey="Sherwood, N M" sort="Sherwood, N M" uniqKey="Sherwood N" first="N M" last="Sherwood">N M Sherwood</name>
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<author><name sortKey="Lescheid, D W" sort="Lescheid, D W" uniqKey="Lescheid D" first="D W" last="Lescheid">D W Lescheid</name>
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<author><name sortKey="Powell, J F" sort="Powell, J F" uniqKey="Powell J" first="J F" last="Powell">J F Powell</name>
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<author><name sortKey="Fischer, W H" sort="Fischer, W H" uniqKey="Fischer W" first="W H" last="Fischer">W H Fischer</name>
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<author><name sortKey="Park, M" sort="Park, M" uniqKey="Park M" first="M" last="Park">M. Park</name>
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<author><name sortKey="Craig, A" sort="Craig, A" uniqKey="Craig A" first="A" last="Craig">A. Craig</name>
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<author><name sortKey="Bukovskaya, O" sort="Bukovskaya, O" uniqKey="Bukovskaya O" first="O" last="Bukovskaya">O. Bukovskaya</name>
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<author><name sortKey="Barannikova, I A" sort="Barannikova, I A" uniqKey="Barannikova I" first="I A" last="Barannikova">I A Barannikova</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Brain Chemistry</term>
<term>Chromatography, High Pressure Liquid</term>
<term>Female</term>
<term>Fishes</term>
<term>Gonadotropin-Releasing Hormone (analysis)</term>
<term>Gonadotropin-Releasing Hormone (chemistry)</term>
<term>Male</term>
<term>Mass Spectrometry</term>
<term>Molecular Sequence Data</term>
<term>Sequence Analysis</term>
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<term>Animals</term>
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<term>Female</term>
<term>Fishes</term>
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<front><div type="abstract" xml:lang="en">The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl amino-peptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.</div>
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Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti

Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti

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