Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti.
Identifieur interne : 000851 ( Ncbi/Curation ); précédent : 000850; suivant : 000852Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti.
Auteurs : D W Lescheid [Canada] ; J F Powell ; W H Fischer ; M. Park ; A. Craig ; O. Bukovskaya ; I A Barannikova ; N M SherwoodSource :
- Regulatory peptides [ 0167-0115 ] ; 1995.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Gonadotropin-Releasing Hormone.
- chemical , chemistry : Gonadotropin-Releasing Hormone.
- Amino Acid Sequence, Animals, Brain Chemistry, Chromatography, High Pressure Liquid, Female, Fishes, Male, Mass Spectrometry, Molecular Sequence Data, Sequence Analysis.
Abstract
The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl amino-peptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.
PubMed: 7761629
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Park</name></author><author><name sortKey="Craig, A" sort="Craig, A" uniqKey="Craig A" first="A" last="Craig">A. Craig</name></author><author><name sortKey="Bukovskaya, O" sort="Bukovskaya, O" uniqKey="Bukovskaya O" first="O" last="Bukovskaya">O. Bukovskaya</name></author><author><name sortKey="Barannikova, I A" sort="Barannikova, I A" uniqKey="Barannikova I" first="I A" last="Barannikova">I A Barannikova</name></author><author><name sortKey="Sherwood, N M" sort="Sherwood, N M" uniqKey="Sherwood N" first="N M" last="Sherwood">N M Sherwood</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1995">1995</date><idno type="RBID">pubmed:7761629</idno><idno type="pmid">7761629</idno><idno type="wicri:Area/PubMed/Corpus">000703</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000703</idno><idno type="wicri:Area/PubMed/Curation">000703</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000703</idno><idno type="wicri:Area/PubMed/Checkpoint">000703</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000703</idno><idno type="wicri:Area/Ncbi/Merge">000851</idno><idno type="wicri:Area/Ncbi/Curation">000851</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti.</title><author><name sortKey="Lescheid, D W" sort="Lescheid, D W" uniqKey="Lescheid D" first="D W" last="Lescheid">D W Lescheid</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, University of Victoria, B.C., Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, University of Victoria, B.C.</wicri:regionArea><wicri:noRegion>B.C.</wicri:noRegion></affiliation></author><author><name sortKey="Powell, J F" sort="Powell, J F" uniqKey="Powell J" first="J F" last="Powell">J F Powell</name></author><author><name sortKey="Fischer, W H" sort="Fischer, W H" uniqKey="Fischer W" first="W H" last="Fischer">W H Fischer</name></author><author><name sortKey="Park, M" sort="Park, M" uniqKey="Park M" first="M" last="Park">M. Park</name></author><author><name sortKey="Craig, A" sort="Craig, A" uniqKey="Craig A" first="A" last="Craig">A. Craig</name></author><author><name sortKey="Bukovskaya, O" sort="Bukovskaya, O" uniqKey="Bukovskaya O" first="O" last="Bukovskaya">O. Bukovskaya</name></author><author><name sortKey="Barannikova, I A" sort="Barannikova, I A" uniqKey="Barannikova I" first="I A" last="Barannikova">I A Barannikova</name></author><author><name sortKey="Sherwood, N M" sort="Sherwood, N M" uniqKey="Sherwood N" first="N M" last="Sherwood">N M Sherwood</name></author></analytic><series><title level="j">Regulatory peptides</title><idno type="ISSN">0167-0115</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Brain Chemistry</term><term>Chromatography, High Pressure Liquid</term><term>Female</term><term>Fishes</term><term>Gonadotropin-Releasing Hormone (analysis)</term><term>Gonadotropin-Releasing Hormone (chemistry)</term><term>Male</term><term>Mass Spectrometry</term><term>Molecular Sequence Data</term><term>Sequence Analysis</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Gonadotropin-Releasing Hormone</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Gonadotropin-Releasing Hormone</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Brain Chemistry</term><term>Chromatography, High Pressure Liquid</term><term>Female</term><term>Fishes</term><term>Male</term><term>Mass Spectrometry</term><term>Molecular Sequence Data</term><term>Sequence Analysis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl amino-peptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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