Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti
Identifieur interne : 000598 ( PascalFrancis/Curation ); précédent : 000597; suivant : 000599Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti
Auteurs : D. W. Lescheid [Canada] ; J. F. F. Powell [Canada] ; W. H. Fischer ; M. Park ; A. Craig ; O. Bukovskaya ; I. A. Barannikova ; N. M. Sherwood [Canada]Source :
- Regulatory peptides [ 0167-0115 ] ; 1995.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl aminopeptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
pA |
|
---|
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000513
Links to Exploration step
Pascal:95-0193486Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti</title>
<author><name sortKey="Lescheid, D W" sort="Lescheid, D W" uniqKey="Lescheid D" first="D. W." last="Lescheid">D. W. Lescheid</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. Victoria, dep. biology</s1>
<s2>Victoria BC V8W 2Y2</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Powell, J F F" sort="Powell, J F F" uniqKey="Powell J" first="J. F. F." last="Powell">J. F. F. Powell</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. Victoria, dep. biology</s1>
<s2>Victoria BC V8W 2Y2</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Fischer, W H" sort="Fischer, W H" uniqKey="Fischer W" first="W. H." last="Fischer">W. H. Fischer</name>
</author>
<author><name sortKey="Park, M" sort="Park, M" uniqKey="Park M" first="M." last="Park">M. Park</name>
</author>
<author><name sortKey="Craig, A" sort="Craig, A" uniqKey="Craig A" first="A." last="Craig">A. Craig</name>
</author>
<author><name sortKey="Bukovskaya, O" sort="Bukovskaya, O" uniqKey="Bukovskaya O" first="O." last="Bukovskaya">O. Bukovskaya</name>
</author>
<author><name sortKey="Barannikova, I A" sort="Barannikova, I A" uniqKey="Barannikova I" first="I. A." last="Barannikova">I. A. Barannikova</name>
</author>
<author><name sortKey="Sherwood, N M" sort="Sherwood, N M" uniqKey="Sherwood N" first="N. M." last="Sherwood">N. M. Sherwood</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. Victoria, dep. biology</s1>
<s2>Victoria BC V8W 2Y2</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">95-0193486</idno>
<date when="1995">1995</date>
<idno type="stanalyst">PASCAL 95-0193486 INIST</idno>
<idno type="RBID">Pascal:95-0193486</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000513</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000598</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti</title>
<author><name sortKey="Lescheid, D W" sort="Lescheid, D W" uniqKey="Lescheid D" first="D. W." last="Lescheid">D. W. Lescheid</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. Victoria, dep. biology</s1>
<s2>Victoria BC V8W 2Y2</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Powell, J F F" sort="Powell, J F F" uniqKey="Powell J" first="J. F. F." last="Powell">J. F. F. Powell</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. Victoria, dep. biology</s1>
<s2>Victoria BC V8W 2Y2</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Fischer, W H" sort="Fischer, W H" uniqKey="Fischer W" first="W. H." last="Fischer">W. H. Fischer</name>
</author>
<author><name sortKey="Park, M" sort="Park, M" uniqKey="Park M" first="M." last="Park">M. Park</name>
</author>
<author><name sortKey="Craig, A" sort="Craig, A" uniqKey="Craig A" first="A." last="Craig">A. Craig</name>
</author>
<author><name sortKey="Bukovskaya, O" sort="Bukovskaya, O" uniqKey="Bukovskaya O" first="O." last="Bukovskaya">O. Bukovskaya</name>
</author>
<author><name sortKey="Barannikova, I A" sort="Barannikova, I A" uniqKey="Barannikova I" first="I. A." last="Barannikova">I. A. Barannikova</name>
</author>
<author><name sortKey="Sherwood, N M" sort="Sherwood, N M" uniqKey="Sherwood N" first="N. M." last="Sherwood">N. M. Sherwood</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. Victoria, dep. biology</s1>
<s2>Victoria BC V8W 2Y2</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Regulatory peptides</title>
<title level="j" type="abbreviated">Regul. pept.</title>
<idno type="ISSN">0167-0115</idno>
<imprint><date when="1995">1995</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Regulatory peptides</title>
<title level="j" type="abbreviated">Regul. pept.</title>
<idno type="ISSN">0167-0115</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aminoacid sequence</term>
<term>Biological evolution</term>
<term>Gonadotropin RH</term>
<term>Purification</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Gonadotrophine RH</term>
<term>Purification</term>
<term>Séquence aminoacide</term>
<term>Evolution biologique</term>
<term>Acipenser gueldenstaedti</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl aminopeptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH<sub>2</sub>
</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0167-0115</s0>
</fA01>
<fA02 i1="01"><s0>REPPDY</s0>
</fA02>
<fA03 i2="1"><s0>Regul. pept.</s0>
</fA03>
<fA05><s2>55</s2>
</fA05>
<fA06><s2>3</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>LESCHEID (D. W.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>POWELL (J. F. F.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>FISCHER (W. H.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>PARK (M.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>CRAIG (A.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>BUKOVSKAYA (O.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>BARANNIKOVA (I. A.)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>SHERWOOD (N. M.)</s1>
</fA11>
<fA14 i1="01"><s1>Univ. Victoria, dep. biology</s1>
<s2>Victoria BC V8W 2Y2</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA20><s1>299-309</s1>
</fA20>
<fA21><s1>1995</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>18854</s2>
<s5>354000059289330090</s5>
</fA43>
<fA44><s0>0000</s0>
</fA44>
<fA45><s0>50 ref.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>95-0193486</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Regulatory peptides</s0>
</fA64>
<fA66 i1="01"><s0>NLD</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl aminopeptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH<sub>2</sub>
</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A02D02</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Gonadotrophine RH</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Gonadotropin RH</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Gonadotropina RH</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Purification</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Purification</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Purificación</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Séquence aminoacide</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Aminoacid sequence</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Secuencia aminoácido</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Evolution biologique</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Biological evolution</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Evolución biológica</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Acipenser gueldenstaedti</s0>
<s2>NS</s2>
<s4>INC</s4>
<s5>64</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Milieu marin</s0>
<s5>16</s5>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Marine environment</s0>
<s5>16</s5>
</fC07>
<fC07 i1="01" i2="X" l="GER"><s0>Meerumwelt</s0>
<s5>16</s5>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Medio marino</s0>
<s5>16</s5>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Neuropeptide</s0>
<s5>17</s5>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Neuropeptide</s0>
<s5>17</s5>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Neuropéptido</s0>
<s5>17</s5>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Hormone hypothalamique</s0>
<s5>18</s5>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Hypothalamic hormone</s0>
<s5>18</s5>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Hormona hipotalámica</s0>
<s5>18</s5>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Pisces</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Pisces</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Pisces</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Acipenseridae</s0>
<s2>NS</s2>
<s4>INC</s4>
<s5>71</s5>
</fC07>
<fN21><s1>116</s1>
</fN21>
</pA>
</standard>
</inist>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/PascalFrancis/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000598 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PascalFrancis/Curation/biblio.hfd -nk 000598 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= PascalFrancis |étape= Curation |type= RBID |clé= Pascal:95-0193486 |texte= Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti }}
![]() | This area was generated with Dilib version V0.6.27. | ![]() |