EsturgeonV1, PascalFrancis, Curation, bibRecord, 000598

Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti

Identifieur interne : 000598 ( PascalFrancis/Curation ); précédent : 000597; suivant : 000599

Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti

Auteurs : D. W. Lescheid [Canada] ; J. F. F. Powell [Canada] ; W. H. Fischer ; M. Park ; A. Craig ; O. Bukovskaya ; I. A. Barannikova ; N. M. Sherwood [Canada]

Source :

Regulatory peptides [ 0167-0115 ] ; 1995.

RBID : Pascal:95-0193486

Descripteurs français

Pascal (Inist)
- Gonadotrophine RH, Purification, Séquence aminoacide, Evolution biologique, Acipenser gueldenstaedti.

English descriptors

KwdEn :
- Aminoacid sequence, Biological evolution, Gonadotropin RH, Purification.

Abstract

The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl aminopeptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH₂

A01	`01`	`1`		`@0 0167-0115`
A02	`01`			`@0 REPPDY`
A03		`1`		`@0 Regul. pept.`
A05				`@2 55`
A06				`@2 3`
A08	`01`	`1`	`ENG`	`@1 Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti`
A11	`01`	`1`		`@1 LESCHEID (D. W.)`
A11	`02`	`1`		`@1 POWELL (J. F. F.)`
A11	`03`	`1`		`@1 FISCHER (W. H.)`
A11	`04`	`1`		`@1 PARK (M.)`
A11	`05`	`1`		`@1 CRAIG (A.)`
A11	`06`	`1`		`@1 BUKOVSKAYA (O.)`
A11	`07`	`1`		`@1 BARANNIKOVA (I. A.)`
A11	`08`	`1`		`@1 SHERWOOD (N. M.)`
A14	`01`			`@1 Univ. Victoria, dep. biology @2 Victoria BC V8W 2Y2 @3 CAN @Z 1 aut. @Z 2 aut. @Z 8 aut.`
A20				`@1 299-309`
A21				`@1 1995`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 18854 @5 354000059289330090`
A44				`@0 0000`
A45				`@0 50 ref.`
A47	`01`	`1`		`@0 95-0193486`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Regulatory peptides`
A66	`01`			`@0 NLD`
C01	`01`		`ENG`	@0 The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl aminopeptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH₂
C02	`01`	`X`		`@0 002A02D02`
C03	`01`	`X`	`FRE`	`@0 Gonadotrophine RH @5 01`
C03	`01`	`X`	`ENG`	`@0 Gonadotropin RH @5 01`
C03	`01`	`X`	`SPA`	`@0 Gonadotropina RH @5 01`
C03	`02`	`X`	`FRE`	`@0 Purification @5 02`
C03	`02`	`X`	`ENG`	`@0 Purification @5 02`
C03	`02`	`X`	`SPA`	`@0 Purificación @5 02`
C03	`03`	`X`	`FRE`	`@0 Séquence aminoacide @5 03`
C03	`03`	`X`	`ENG`	`@0 Aminoacid sequence @5 03`
C03	`03`	`X`	`SPA`	`@0 Secuencia aminoácido @5 03`
C03	`04`	`X`	`FRE`	`@0 Evolution biologique @5 04`
C03	`04`	`X`	`ENG`	`@0 Biological evolution @5 04`
C03	`04`	`X`	`SPA`	`@0 Evolución biológica @5 04`
C03	`05`	`X`	`FRE`	`@0 Acipenser gueldenstaedti @2 NS @4 INC @5 64`
C07	`01`	`X`	`FRE`	`@0 Milieu marin @5 16`
C07	`01`	`X`	`ENG`	`@0 Marine environment @5 16`
C07	`01`	`X`	`GER`	`@0 Meerumwelt @5 16`
C07	`01`	`X`	`SPA`	`@0 Medio marino @5 16`
C07	`02`	`X`	`FRE`	`@0 Neuropeptide @5 17`
C07	`02`	`X`	`ENG`	`@0 Neuropeptide @5 17`
C07	`02`	`X`	`SPA`	`@0 Neuropéptido @5 17`
C07	`03`	`X`	`FRE`	`@0 Hormone hypothalamique @5 18`
C07	`03`	`X`	`ENG`	`@0 Hypothalamic hormone @5 18`
C07	`03`	`X`	`SPA`	`@0 Hormona hipotalámica @5 18`
C07	`04`	`X`	`FRE`	`@0 Pisces @2 NS @5 29`
C07	`04`	`X`	`ENG`	`@0 Pisces @2 NS @5 29`
C07	`04`	`X`	`SPA`	`@0 Pisces @2 NS @5 29`
C07	`05`	`X`	`FRE`	`@0 Vertebrata @2 NS`
C07	`05`	`X`	`ENG`	`@0 Vertebrata @2 NS`
C07	`05`	`X`	`SPA`	`@0 Vertebrata @2 NS`
C07	`06`	`X`	`FRE`	`@0 Acipenseridae @2 NS @4 INC @5 71`
N21				`@1 116`

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Pascal:95-0193486

Le document en format XML

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<author><name sortKey="Lescheid, D W" sort="Lescheid, D W" uniqKey="Lescheid D" first="D. W." last="Lescheid">D. W. Lescheid</name>
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<s2>Victoria BC V8W 2Y2</s2>
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<country>Canada</country>
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<author><name sortKey="Powell, J F F" sort="Powell, J F F" uniqKey="Powell J" first="J. F. F." last="Powell">J. F. F. Powell</name>
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<author><name sortKey="Fischer, W H" sort="Fischer, W H" uniqKey="Fischer W" first="W. H." last="Fischer">W. H. Fischer</name>
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<author><name sortKey="Park, M" sort="Park, M" uniqKey="Park M" first="M." last="Park">M. Park</name>
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<author><name sortKey="Craig, A" sort="Craig, A" uniqKey="Craig A" first="A." last="Craig">A. Craig</name>
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<author><name sortKey="Bukovskaya, O" sort="Bukovskaya, O" uniqKey="Bukovskaya O" first="O." last="Bukovskaya">O. Bukovskaya</name>
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<author><name sortKey="Barannikova, I A" sort="Barannikova, I A" uniqKey="Barannikova I" first="I. A." last="Barannikova">I. A. Barannikova</name>
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<author><name sortKey="Sherwood, N M" sort="Sherwood, N M" uniqKey="Sherwood N" first="N. M." last="Sherwood">N. M. Sherwood</name>
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<idno type="inist">95-0193486</idno>
<date when="1995">1995</date>
<idno type="stanalyst">PASCAL 95-0193486 INIST</idno>
<idno type="RBID">Pascal:95-0193486</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000513</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000598</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti</title>
<author><name sortKey="Lescheid, D W" sort="Lescheid, D W" uniqKey="Lescheid D" first="D. W." last="Lescheid">D. W. Lescheid</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. Victoria, dep. biology</s1>
<s2>Victoria BC V8W 2Y2</s2>
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<country>Canada</country>
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<author><name sortKey="Powell, J F F" sort="Powell, J F F" uniqKey="Powell J" first="J. F. F." last="Powell">J. F. F. Powell</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. Victoria, dep. biology</s1>
<s2>Victoria BC V8W 2Y2</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Canada</country>
</affiliation>
</author>
<author><name sortKey="Fischer, W H" sort="Fischer, W H" uniqKey="Fischer W" first="W. H." last="Fischer">W. H. Fischer</name>
</author>
<author><name sortKey="Park, M" sort="Park, M" uniqKey="Park M" first="M." last="Park">M. Park</name>
</author>
<author><name sortKey="Craig, A" sort="Craig, A" uniqKey="Craig A" first="A." last="Craig">A. Craig</name>
</author>
<author><name sortKey="Bukovskaya, O" sort="Bukovskaya, O" uniqKey="Bukovskaya O" first="O." last="Bukovskaya">O. Bukovskaya</name>
</author>
<author><name sortKey="Barannikova, I A" sort="Barannikova, I A" uniqKey="Barannikova I" first="I. A." last="Barannikova">I. A. Barannikova</name>
</author>
<author><name sortKey="Sherwood, N M" sort="Sherwood, N M" uniqKey="Sherwood N" first="N. M." last="Sherwood">N. M. Sherwood</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Univ. Victoria, dep. biology</s1>
<s2>Victoria BC V8W 2Y2</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
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<country>Canada</country>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Regulatory peptides</title>
<title level="j" type="abbreviated">Regul. pept.</title>
<idno type="ISSN">0167-0115</idno>
<imprint><date when="1995">1995</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Regulatory peptides</title>
<title level="j" type="abbreviated">Regul. pept.</title>
<idno type="ISSN">0167-0115</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aminoacid sequence</term>
<term>Biological evolution</term>
<term>Gonadotropin RH</term>
<term>Purification</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Gonadotrophine RH</term>
<term>Purification</term>
<term>Séquence aminoacide</term>
<term>Evolution biologique</term>
<term>Acipenser gueldenstaedti</term>
</keywords>
</textClass>
</profileDesc>
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<front><div type="abstract" xml:lang="en">The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl aminopeptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH<sub>2</sub>
</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0167-0115</s0>
</fA01>
<fA02 i1="01"><s0>REPPDY</s0>
</fA02>
<fA03 i2="1"><s0>Regul. pept.</s0>
</fA03>
<fA05><s2>55</s2>
</fA05>
<fA06><s2>3</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti</s1>
</fA08>
<fA11 i1="01" i2="1"><s1>LESCHEID (D. W.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>POWELL (J. F. F.)</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>FISCHER (W. H.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>PARK (M.)</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>CRAIG (A.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>BUKOVSKAYA (O.)</s1>
</fA11>
<fA11 i1="07" i2="1"><s1>BARANNIKOVA (I. A.)</s1>
</fA11>
<fA11 i1="08" i2="1"><s1>SHERWOOD (N. M.)</s1>
</fA11>
<fA14 i1="01"><s1>Univ. Victoria, dep. biology</s1>
<s2>Victoria BC V8W 2Y2</s2>
<s3>CAN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>8 aut.</sZ>
</fA14>
<fA20><s1>299-309</s1>
</fA20>
<fA21><s1>1995</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA43 i1="01"><s1>INIST</s1>
<s2>18854</s2>
<s5>354000059289330090</s5>
</fA43>
<fA44><s0>0000</s0>
</fA44>
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</fA45>
<fA47 i1="01" i2="1"><s0>95-0193486</s0>
</fA47>
<fA60><s1>P</s1>
</fA60>
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</fA64>
<fA66 i1="01"><s0>NLD</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The mammalian form of gonadotropin-releasing hormone (GnRH) was purified from the brains of Russian sturgeon, Acipenser gueldenstaedti, using reversed-phase high pressure liquid chromatography (HPLC). The total concentration of mGnRH within these fish was 5.4 ng/brain. Small amounts of immunoreactive chicken GnRH-II like molecules were also detected but at insufficient quantities for purification. The primary structure of mGnRH was determined using automated Edman degradation. Because sequence data could not be obtained until after digestion by bovine pyroglutamyl aminopeptidase, it was determined that the amino-terminal residue was modified. Furthermore, mass spectrometric data and co-elution with synthetic mGnRH on HPLC confirmed that the carboxy-terminal residue was amidated. The amino acid sequence of sturgeon GnRH is pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH<sub>2</sub>
</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A02D02</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Gonadotrophine RH</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Gonadotropin RH</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Gonadotropina RH</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Purification</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Purification</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Purificación</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Séquence aminoacide</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Aminoacid sequence</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Secuencia aminoácido</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Evolution biologique</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Biological evolution</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Evolución biológica</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Acipenser gueldenstaedti</s0>
<s2>NS</s2>
<s4>INC</s4>
<s5>64</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Milieu marin</s0>
<s5>16</s5>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Marine environment</s0>
<s5>16</s5>
</fC07>
<fC07 i1="01" i2="X" l="GER"><s0>Meerumwelt</s0>
<s5>16</s5>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Medio marino</s0>
<s5>16</s5>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Neuropeptide</s0>
<s5>17</s5>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Neuropeptide</s0>
<s5>17</s5>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Neuropéptido</s0>
<s5>17</s5>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Hormone hypothalamique</s0>
<s5>18</s5>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Hypothalamic hormone</s0>
<s5>18</s5>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Hormona hipotalámica</s0>
<s5>18</s5>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Pisces</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Pisces</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Pisces</s0>
<s2>NS</s2>
<s5>29</s5>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Vertebrata</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Acipenseridae</s0>
<s2>NS</s2>
<s4>INC</s4>
<s5>71</s5>
</fC07>
<fN21><s1>116</s1>
</fN21>
</pA>
</standard>
</inist>
</record>

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Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti

Mammalian gonadotropin-releasing hormone (GnRH) identified by primary structure in Russian sturgeon, Acipenser gueldenstaedti

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