Molecular diagnosis of iridovirus infections in cold-blooded animals
Identifieur interne : 000C53 ( Istex/Curation ); précédent : 000C52; suivant : 000C54Molecular diagnosis of iridovirus infections in cold-blooded animals
Auteurs : V. Gregory Chinchar [États-Unis] ; Jinghe Mao [États-Unis]Source :
- Seminars in Avian and Exotic Pet Medicine [ 1055-937X ] ; 2000.
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Abstract
Until the mid-1980s, iridoviruses infecting cold-blooded vertebrates were viewed as agents responsible for benign, self-limiting infections of fish and amphibians. However, within the last 15 years, iridoviruses (genus Ranavirus) have been linked to die-offs of many different species of marine and freshwater fish as well as to severe disease in anurans and urodeles. Because diagnosis of iridovirus infections based on clinical signs alone is not definitive, a panel of microscopic, serological, and molecular techniques must be used to identify the etiologic agent. Transmission electron microscopy is capable of classifying the agent as a member of the family Iridoviridae. Enzyme-linked immunosorbent assay (ELISA) and other serological tests readily identify the genus Ranavirus, and polymerase chain reaction (PCR) sequence analyses distinguish among different virus species/strains. Although it is not currently possible to treat or prevent most iridovirus disease, identification of the specific disease agent is important in differential diagnosis and in controlling spread of the agent to uninfected populations.
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DOI: 10.1016/S1055-937X(00)80013-4
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USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, University of Mississippi Medical Center, Jackson</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Seminars in Avian and Exotic Pet Medicine</title><title level="j" type="abbrev">YSAEP</title><idno type="ISSN">1055-937X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">9</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="27">27</biblScope><biblScope unit="page" to="35">35</biblScope></imprint><idno type="ISSN">1055-937X</idno></series><idno type="istex">0AE75361FC19D6C5002ED315F837FDBC1FD313A3</idno><idno type="DOI">10.1016/S1055-937X(00)80013-4</idno><idno type="PII">S1055-937X(00)80013-4</idno><idno type="ArticleID">00800134</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1055-937X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Iridovirus</term><term>amphibians</term><term>epizootic hematopoietic necrosis virus</term><term>fish</term><term>molecular diagnosis</term><term>ranavirus</term><term>viruses infecting cold-blooded animals</term><term>viruses infecting ectothermic animals</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Until the mid-1980s, iridoviruses infecting cold-blooded vertebrates were viewed as agents responsible for benign, self-limiting infections of fish and amphibians. However, within the last 15 years, iridoviruses (genus Ranavirus) have been linked to die-offs of many different species of marine and freshwater fish as well as to severe disease in anurans and urodeles. Because diagnosis of iridovirus infections based on clinical signs alone is not definitive, a panel of microscopic, serological, and molecular techniques must be used to identify the etiologic agent. Transmission electron microscopy is capable of classifying the agent as a member of the family Iridoviridae. Enzyme-linked immunosorbent assay (ELISA) and other serological tests readily identify the genus Ranavirus, and polymerase chain reaction (PCR) sequence analyses distinguish among different virus species/strains. Although it is not currently possible to treat or prevent most iridovirus disease, identification of the specific disease agent is important in differential diagnosis and in controlling spread of the agent to uninfected populations.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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