Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout Salmo trutta from northern Italy
Identifieur interne : 001575 ( Istex/Corpus ); précédent : 001574; suivant : 001576Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout Salmo trutta from northern Italy
Auteurs : E. Giuffra ; L. Bernatchez ; R. GuyomardSource :
- Molecular Ecology [ 0962-1083 ] ; 1994-04.
English descriptors
- KwdEn :
Abstract
The Pô River basin of northern Italy is the home of distinctive and endemic morphological forms of brown trout Salmo trutta. We used PCR‐direct sequencing and RFLP techniques to study variation in the mitochondrial control region of 225 trout in order to assess genetic relatedness among 18 populations from that region. The distribution analysis of these genotypes among north Italian populations confirmed the phylogenetic differentiation of marbled trout Salmo trutta marmoratus populations and the postglacial origin of S. t. fario. Extensive genetic heterogeneity was observed among morphologically identical S. t. fario populations. Introgression with domestic strains of Atlantic basin origin was detected in all forms. In order to assess the phylogenetic congruence detected in coding and noncoding regions of the mitochondrial genome, we also analysed sequence variation in segments of the cytochrome b and ATPase subunit VI genes among representatives of all variants detected in the analysis of the control region. Variation in protein coding genes was only slightly less than that observed in the control region of the same individuals, both in terms of number of variants detected and of pairwise sequence divergence estimates among variants. Phylogenetic analysis based on protein coding genes sequences identified the same phylogenetic groupings defined by the control region analysis and also allowed a partial resolution of their phyletic relationships that was previously unresolved. However, coding and noncoding segments differed substantially in the transition‐transversion ratio (17:0 in coding segments vs. 17:6 in control region segments).
Url:
DOI: 10.1111/j.1365-294X.1994.tb00117.x
Links to Exploration step
ISTEX:B313728FAAAB0BCB75AC3E385596C86AD069B4E5Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout Salmo trutta from northern Italy</title><author><name sortKey="Giuffra, E" sort="Giuffra, E" uniqKey="Giuffra E" first="E." last="Giuffra">E. Giuffra</name><affiliation><mods:affiliation>Laboratoire de Génétique des Poissons, INRA, 78352 Jouy‐en‐Josas, France</mods:affiliation></affiliation></author><author><name sortKey="Bernatchez, L" sort="Bernatchez, L" uniqKey="Bernatchez L" first="L." last="Bernatchez">L. Bernatchez</name><affiliation><mods:affiliation>*INRS‐Eau, 2800 rue Einstein, suite 105, CP 7500, Sainte‐Foy, Québec, Canada G1V 4C7</mods:affiliation></affiliation><affiliation><mods:affiliation>INRS‐Eau, 2800 rue Einstein, suite 105, CP 7500, Sainte‐Foy (Québec), Canada G1V 4C7. Fax (418)6542600.</mods:affiliation></affiliation></author><author><name sortKey="Guyomard, R" sort="Guyomard, R" uniqKey="Guyomard R" first="R." last="Guyomard">R. Guyomard</name><affiliation><mods:affiliation>Laboratoire de Génétique des Poissons, INRA, 78352 Jouy‐en‐Josas, France</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:B313728FAAAB0BCB75AC3E385596C86AD069B4E5</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1111/j.1365-294X.1994.tb00117.x</idno><idno type="url">https://api.istex.fr/document/B313728FAAAB0BCB75AC3E385596C86AD069B4E5/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001575</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001575</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout Salmo trutta from northern Italy</title><author><name sortKey="Giuffra, E" sort="Giuffra, E" uniqKey="Giuffra E" first="E." last="Giuffra">E. Giuffra</name><affiliation><mods:affiliation>Laboratoire de Génétique des Poissons, INRA, 78352 Jouy‐en‐Josas, France</mods:affiliation></affiliation></author><author><name sortKey="Bernatchez, L" sort="Bernatchez, L" uniqKey="Bernatchez L" first="L." last="Bernatchez">L. Bernatchez</name><affiliation><mods:affiliation>*INRS‐Eau, 2800 rue Einstein, suite 105, CP 7500, Sainte‐Foy, Québec, Canada G1V 4C7</mods:affiliation></affiliation><affiliation><mods:affiliation>INRS‐Eau, 2800 rue Einstein, suite 105, CP 7500, Sainte‐Foy (Québec), Canada G1V 4C7. Fax (418)6542600.</mods:affiliation></affiliation></author><author><name sortKey="Guyomard, R" sort="Guyomard, R" uniqKey="Guyomard R" first="R." last="Guyomard">R. Guyomard</name><affiliation><mods:affiliation>Laboratoire de Génétique des Poissons, INRA, 78352 Jouy‐en‐Josas, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Ecology</title><idno type="ISSN">0962-1083</idno><idno type="eISSN">1365-294X</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1994-04">1994-04</date><biblScope unit="volume">3</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="161">161</biblScope><biblScope unit="page" to="171">171</biblScope></imprint><idno type="ISSN">0962-1083</idno></series><idno type="istex">B313728FAAAB0BCB75AC3E385596C86AD069B4E5</idno><idno type="DOI">10.1111/j.1365-294X.1994.tb00117.x</idno><idno type="ArticleID">MEC161</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0962-1083</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>S. trutta</term><term>control region</term><term>mitochondrial DNA</term><term>phylogeny</term><term>polymerase chain reaction</term><term>protein coding genes</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Pô River basin of northern Italy is the home of distinctive and endemic morphological forms of brown trout Salmo trutta. We used PCR‐direct sequencing and RFLP techniques to study variation in the mitochondrial control region of 225 trout in order to assess genetic relatedness among 18 populations from that region. The distribution analysis of these genotypes among north Italian populations confirmed the phylogenetic differentiation of marbled trout Salmo trutta marmoratus populations and the postglacial origin of S. t. fario. Extensive genetic heterogeneity was observed among morphologically identical S. t. fario populations. Introgression with domestic strains of Atlantic basin origin was detected in all forms. In order to assess the phylogenetic congruence detected in coding and noncoding regions of the mitochondrial genome, we also analysed sequence variation in segments of the cytochrome b and ATPase subunit VI genes among representatives of all variants detected in the analysis of the control region. Variation in protein coding genes was only slightly less than that observed in the control region of the same individuals, both in terms of number of variants detected and of pairwise sequence divergence estimates among variants. Phylogenetic analysis based on protein coding genes sequences identified the same phylogenetic groupings defined by the control region analysis and also allowed a partial resolution of their phyletic relationships that was previously unresolved. However, coding and noncoding segments differed substantially in the transition‐transversion ratio (17:0 in coding segments vs. 17:6 in control region segments).</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>E. GIUFFRA</name><affiliations><json:string>Laboratoire de Génétique des Poissons, INRA, 78352 Jouy‐en‐Josas, France</json:string></affiliations></json:item><json:item><name>L. BERNATCHEZ</name><affiliations><json:string>*INRS‐Eau, 2800 rue Einstein, suite 105, CP 7500, Sainte‐Foy, Québec, Canada G1V 4C7</json:string><json:string>INRS‐Eau, 2800 rue Einstein, suite 105, CP 7500, Sainte‐Foy (Québec), Canada G1V 4C7. Fax (418)6542600.</json:string></affiliations></json:item><json:item><name>R. GUYOMARD</name><affiliations><json:string>Laboratoire de Génétique des Poissons, INRA, 78352 Jouy‐en‐Josas, France</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>control region</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>mitochondrial DNA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>phylogeny</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>polymerase chain reaction</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>protein coding genes</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>S. trutta</value></json:item></subject><articleId><json:string>MEC161</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>The Pô River basin of northern Italy is the home of distinctive and endemic morphological forms of brown trout Salmo trutta. We used PCR‐direct sequencing and RFLP techniques to study variation in the mitochondrial control region of 225 trout in order to assess genetic relatedness among 18 populations from that region. The distribution analysis of these genotypes among north Italian populations confirmed the phylogenetic differentiation of marbled trout Salmo trutta marmoratus populations and the postglacial origin of S. t. fario. Extensive genetic heterogeneity was observed among morphologically identical S. t. fario populations. Introgression with domestic strains of Atlantic basin origin was detected in all forms. In order to assess the phylogenetic congruence detected in coding and noncoding regions of the mitochondrial genome, we also analysed sequence variation in segments of the cytochrome b and ATPase subunit VI genes among representatives of all variants detected in the analysis of the control region. Variation in protein coding genes was only slightly less than that observed in the control region of the same individuals, both in terms of number of variants detected and of pairwise sequence divergence estimates among variants. Phylogenetic analysis based on protein coding genes sequences identified the same phylogenetic groupings defined by the control region analysis and also allowed a partial resolution of their phyletic relationships that was previously unresolved. However, coding and noncoding segments differed substantially in the transition‐transversion ratio (17:0 in coding segments vs. 17:6 in control region segments).</abstract><qualityIndicators><score>8.392</score><pdfVersion>1.4</pdfVersion><pdfPageSize>612 x 774.24 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractCharCount>1663</abstractCharCount><pdfWordCount>6322</pdfWordCount><pdfCharCount>40650</pdfCharCount><pdfPageCount>11</pdfPageCount><abstractWordCount>241</abstractWordCount></qualityIndicators><title>Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout Salmo trutta from northern Italy</title><genre><json:string>article</json:string></genre><host><volume>3</volume><publisherId><json:string>MEC</json:string></publisherId><pages><total>11</total><last>171</last><first>161</first></pages><issn><json:string>0962-1083</json:string></issn><issue>2</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1365-294X</json:string></eissn><title>Molecular Ecology</title><doi><json:string>10.1111/(ISSN)1365-294X</json:string></doi></host><categories><wos><json:string>science</json:string><json:string>evolutionary biology</json:string><json:string>ecology</json:string><json:string>biochemistry & molecular biology</json:string></wos><scienceMetrix><json:string>natural sciences</json:string><json:string>biology</json:string><json:string>evolutionary biology</json:string></scienceMetrix></categories><publicationDate>1994</publicationDate><copyrightDate>1994</copyrightDate><doi><json:string>10.1111/j.1365-294X.1994.tb00117.x</json:string></doi><id>B313728FAAAB0BCB75AC3E385596C86AD069B4E5</id><score>0.017558502</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/B313728FAAAB0BCB75AC3E385596C86AD069B4E5/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/B313728FAAAB0BCB75AC3E385596C86AD069B4E5/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/B313728FAAAB0BCB75AC3E385596C86AD069B4E5/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout Salmo trutta from northern Italy</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><availability><p>WILEY</p></availability><date>1994</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout Salmo trutta from northern Italy</title><author xml:id="author-1"><persName><forename type="first">E.</forename><surname>GIUFFRA</surname></persName><affiliation>Laboratoire de Génétique des Poissons, INRA, 78352 Jouy‐en‐Josas, France</affiliation></author><author xml:id="author-2"><persName><forename type="first">L.</forename><surname>BERNATCHEZ</surname></persName><affiliation>*INRS‐Eau, 2800 rue Einstein, suite 105, CP 7500, Sainte‐Foy, Québec, Canada G1V 4C7</affiliation><affiliation>INRS‐Eau, 2800 rue Einstein, suite 105, CP 7500, Sainte‐Foy (Québec), Canada G1V 4C7. Fax (418)6542600.</affiliation></author><author xml:id="author-3"><persName><forename type="first">R.</forename><surname>GUYOMARD</surname></persName><affiliation>Laboratoire de Génétique des Poissons, INRA, 78352 Jouy‐en‐Josas, France</affiliation></author></analytic><monogr><title level="j">Molecular Ecology</title><idno type="pISSN">0962-1083</idno><idno type="eISSN">1365-294X</idno><idno type="DOI">10.1111/(ISSN)1365-294X</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1994-04"></date><biblScope unit="volume">3</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="161">161</biblScope><biblScope unit="page" to="171">171</biblScope></imprint></monogr><idno type="istex">B313728FAAAB0BCB75AC3E385596C86AD069B4E5</idno><idno type="DOI">10.1111/j.1365-294X.1994.tb00117.x</idno><idno type="ArticleID">MEC161</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1994</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The Pô River basin of northern Italy is the home of distinctive and endemic morphological forms of brown trout Salmo trutta. We used PCR‐direct sequencing and RFLP techniques to study variation in the mitochondrial control region of 225 trout in order to assess genetic relatedness among 18 populations from that region. The distribution analysis of these genotypes among north Italian populations confirmed the phylogenetic differentiation of marbled trout Salmo trutta marmoratus populations and the postglacial origin of S. t. fario. Extensive genetic heterogeneity was observed among morphologically identical S. t. fario populations. Introgression with domestic strains of Atlantic basin origin was detected in all forms. In order to assess the phylogenetic congruence detected in coding and noncoding regions of the mitochondrial genome, we also analysed sequence variation in segments of the cytochrome b and ATPase subunit VI genes among representatives of all variants detected in the analysis of the control region. Variation in protein coding genes was only slightly less than that observed in the control region of the same individuals, both in terms of number of variants detected and of pairwise sequence divergence estimates among variants. Phylogenetic analysis based on protein coding genes sequences identified the same phylogenetic groupings defined by the control region analysis and also allowed a partial resolution of their phyletic relationships that was previously unresolved. However, coding and noncoding segments differed substantially in the transition‐transversion ratio (17:0 in coding segments vs. 17:6 in control region segments).</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>control region</term></item><item><term>mitochondrial DNA</term></item><item><term>phylogeny</term></item><item><term>polymerase chain reaction</term></item><item><term>protein coding genes</term></item><item><term>S. trutta</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1994-04">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/B313728FAAAB0BCB75AC3E385596C86AD069B4E5/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Blackwell Publishing Ltd</publisherName><publisherLoc>Oxford, UK</publisherLoc></publisherInfo><doi origin="wiley" registered="yes">10.1111/(ISSN)1365-294X</doi><issn type="print">0962-1083</issn><issn type="electronic">1365-294X</issn><idGroup><id type="product" value="MEC"></id><id type="publisherDivision" value="ST"></id></idGroup><titleGroup><title type="main" sort="MOLECULAR ECOLOGY">Molecular Ecology</title></titleGroup></publicationMeta><publicationMeta level="part" position="04002"><doi origin="wiley">10.1111/mec.1994.3.issue-2</doi><numberingGroup><numbering type="journalVolume" number="3">3</numbering><numbering type="journalIssue" number="2">2</numbering></numberingGroup><coverDate startDate="1994-04">April 1994</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="0016100" status="forIssue"><doi origin="wiley">10.1111/j.1365-294X.1994.tb00117.x</doi><idGroup><id type="unit" value="MEC161"></id></idGroup><countGroup><count type="pageTotal" number="11"></count></countGroup><titleGroup><title type="tocHeading1">Original Article</title></titleGroup><eventGroup><event type="firstOnline" date="2008-10-09"></event><event type="publishedOnlineFinalForm" date="2008-10-09"></event><event type="xmlConverted" agent="Converter:BPG_TO_WML3G version:2.3.6 mode:FullText source:HeaderRef result:HeaderRef" date="2010-04-21"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-20"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-31"></event></eventGroup><numberingGroup><numbering type="pageFirst" number="161">161</numbering><numbering type="pageLast" number="171">171</numbering></numberingGroup><correspondenceTo>INRS‐Eau, 2800 rue Einstein, suite 105, CP 7500, Sainte‐Foy (Québec), Canada G1V 4C7. Fax (418)6542600.</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:MEC.MEC161.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received 8 July 1993; revision received 1 October 1993; accepted 11 November 1993</unparsedEditorialHistory><countGroup><count type="referenceTotal" number="37"></count><count type="linksCrossRef" number="4"></count></countGroup><titleGroup><title type="main">Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout <i>Salmo trutta</i> from northern Italy</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1"><personName><givenNames>E.</givenNames><familyName>GIUFFRA</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a2" corresponding="yes"><personName><givenNames>L.</givenNames><familyName>BERNATCHEZ</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>R.</givenNames><familyName>GUYOMARD</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="FR"><unparsedAffiliation>Laboratoire de Génétique des Poissons, INRA, 78352 Jouy‐en‐Josas, France</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="CA"><unparsedAffiliation>*INRS‐Eau, 2800 rue Einstein, suite 105, CP 7500, Sainte‐Foy, Québec, Canada G1V 4C7</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">control region</keyword><keyword xml:id="k2">mitochondrial DNA</keyword><keyword xml:id="k3">phylogeny</keyword><keyword xml:id="k4">polymerase chain reaction</keyword><keyword xml:id="k5">protein coding genes</keyword><keyword xml:id="k6"><i>S. trutta</i></keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>The Pô River basin of northern Italy is the home of distinctive and endemic morphological forms of brown trout <i>Salmo trutta.</i> We used PCR‐direct sequencing and RFLP techniques to study variation in the mitochondrial control region of 225 trout in order to assess genetic relatedness among 18 populations from that region. The distribution analysis of these genotypes among north Italian populations confirmed the phylogenetic differentiation of marbled trout <i>Salmo trutta marmoratus</i> populations and the postglacial origin of <i>S. t. fario.</i> Extensive genetic heterogeneity was observed among morphologically identical <i>S. t. fario</i> populations. Introgression with domestic strains of Atlantic basin origin was detected in all forms. In order to assess the phylogenetic congruence detected in coding and noncoding regions of the mitochondrial genome, we also analysed sequence variation in segments of the cytochrome <i>b</i> and ATPase subunit VI genes among representatives of all variants detected in the analysis of the control region. Variation in protein coding genes was only slightly less than that observed in the control region of the same individuals, both in terms of number of variants detected and of pairwise sequence divergence estimates among variants. Phylogenetic analysis based on protein coding genes sequences identified the same phylogenetic groupings defined by the control region analysis and also allowed a partial resolution of their phyletic relationships that was previously unresolved. However, coding and noncoding segments differed substantially in the transition‐transversion ratio (17:0 in coding segments vs. 17:6 in control region segments).</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="n-fnt-1" numbered="no"><p>This study is part of the dissertation work of E. Giuffra on the phylogenetic analysis of north Italian brown trout populations based on mitochondrial and nuclear genes data. A major research interest of L. Bernatchez and R. Guyomard is in using molecular genetics approaches to study evolutionary processes and population structure in fish. The collaboration of L. Bernatchez to this study began while he was a postdoctoral fellow in the Laboratoire Genomé et Populations directed by François Bonhomme at Université de Montpellier II, France.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout Salmo trutta from northern Italy</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout Salmo trutta from northern Italy</title></titleInfo><name type="personal"><namePart type="given">E.</namePart><namePart type="family">GIUFFRA</namePart><affiliation>Laboratoire de Génétique des Poissons, INRA, 78352 Jouy‐en‐Josas, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">BERNATCHEZ</namePart><affiliation>*INRS‐Eau, 2800 rue Einstein, suite 105, CP 7500, Sainte‐Foy, Québec, Canada G1V 4C7</affiliation><affiliation>INRS‐Eau, 2800 rue Einstein, suite 105, CP 7500, Sainte‐Foy (Québec), Canada G1V 4C7. Fax (418)6542600.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R.</namePart><namePart type="family">GUYOMARD</namePart><affiliation>Laboratoire de Génétique des Poissons, INRA, 78352 Jouy‐en‐Josas, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1994-04</dateIssued><edition>Received 8 July 1993; revision received 1 October 1993; accepted 11 November 1993</edition><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="references">37</extent></physicalDescription><abstract lang="en">The Pô River basin of northern Italy is the home of distinctive and endemic morphological forms of brown trout Salmo trutta. We used PCR‐direct sequencing and RFLP techniques to study variation in the mitochondrial control region of 225 trout in order to assess genetic relatedness among 18 populations from that region. The distribution analysis of these genotypes among north Italian populations confirmed the phylogenetic differentiation of marbled trout Salmo trutta marmoratus populations and the postglacial origin of S. t. fario. Extensive genetic heterogeneity was observed among morphologically identical S. t. fario populations. Introgression with domestic strains of Atlantic basin origin was detected in all forms. In order to assess the phylogenetic congruence detected in coding and noncoding regions of the mitochondrial genome, we also analysed sequence variation in segments of the cytochrome b and ATPase subunit VI genes among representatives of all variants detected in the analysis of the control region. Variation in protein coding genes was only slightly less than that observed in the control region of the same individuals, both in terms of number of variants detected and of pairwise sequence divergence estimates among variants. Phylogenetic analysis based on protein coding genes sequences identified the same phylogenetic groupings defined by the control region analysis and also allowed a partial resolution of their phyletic relationships that was previously unresolved. However, coding and noncoding segments differed substantially in the transition‐transversion ratio (17:0 in coding segments vs. 17:6 in control region segments).</abstract><subject lang="en"><genre>keywords</genre><topic>control region</topic><topic>mitochondrial DNA</topic><topic>phylogeny</topic><topic>polymerase chain reaction</topic><topic>protein coding genes</topic><topic>S. trutta</topic></subject><relatedItem type="host"><titleInfo><title>Molecular Ecology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0962-1083</identifier><identifier type="eISSN">1365-294X</identifier><identifier type="DOI">10.1111/(ISSN)1365-294X</identifier><identifier type="PublisherID">MEC</identifier><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>3</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>161</start><end>171</end><total>11</total></extent></part></relatedItem><identifier type="istex">B313728FAAAB0BCB75AC3E385596C86AD069B4E5</identifier><identifier type="DOI">10.1111/j.1365-294X.1994.tb00117.x</identifier><identifier type="ArticleID">MEC161</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001575 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001575 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:B313728FAAAB0BCB75AC3E385596C86AD069B4E5
|texte= Mitochondrial control region and protein coding genes sequence variation among phenotypic forms of brown trout Salmo trutta from northern Italy
}}
| This area was generated with Dilib version V0.6.27. |  |