Development of three distinct GnRH neuron populations expressing two different GnRH forms in the brain of the African catfish (Clarias gariepinus)
Identifieur interne : 001369 ( Istex/Corpus ); précédent : 001368; suivant : 001370Development of three distinct GnRH neuron populations expressing two different GnRH forms in the brain of the African catfish (Clarias gariepinus)
Auteurs : Eline A. Dubois ; Matthijs A. Zandbergen ; Jan Peute ; Jan Bogerd ; Henk J. Th. GoosSource :
- Journal of Comparative Neurology [ 0021-9967 ] ; 2001-08-27.
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Abstract
The early development of both the catfish gonadotropin‐releasing hormone (cfGnRH)‐ and the chicken GnRH‐II (cGnRH‐II) system was investigated in African catfish by immunocytochemistry by using antibodies against the GnRH‐associated peptide (GAP) of the respective preprohormones. Weakly cfGnRH‐immunoreactive (ir) neurons and fibers were present at 2 weeks after hatching (ph) but only in the ventral telencephalon and pituitary. Two weeks later, cfGnRH fibers and neurons were also observed in more rostral and in more caudal brain areas, mainly in the preoptic area and hypothalamus. Based on differences in temporal, spatial, and morphologic appearance, two distinct cfGnRH populations were identified in the ventral forebrain: a population innervating the pituitary (ventral forebrain system) and a so‐called terminal nerve (TN) population. DiI tracing studies revealed that the TN population has no neuronal connections with the pituitary. The cGnRH‐II system is present from 2 weeks ph onward in the midbrain tegmentum and only their size and staining intensity increased during development. Based on the comparison of GnRH systems amongst vertebrates, we hypothesize that during fish evolution, three different GnRH systems evolved, each expressing their own molecular form: the cGnRH‐II system in the midbrain, a hypophysiotropic GnRH system in the hypothalamus with a species‐specific GnRH form, and a salmon GnRH‐expressing TN population. This hypothesis is supported by phylogenetic analysis of known GnRH precursor amino acid sequences. We hypothesize, because the African catfish is a less advanced teleost species, that it contains the cfGnRH form both in the ventral forebrain system and in the TN population.J. Comp. Neurol. 437:308–320, 2001. © 2001 Wiley‐Liss, Inc.
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DOI: 10.1002/cne.1285
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ISTEX:2679B0934514CCBF4D3AA7FD1250AFDAA36E08F9Le document en format XML
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Neurol.</title><idno type="ISSN">0021-9967</idno><idno type="eISSN">1096-9861</idno><imprint><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="2001-08-27">2001-08-27</date><biblScope unit="volume">437</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="308">308</biblScope><biblScope unit="page" to="320">320</biblScope></imprint><idno type="ISSN">0021-9967</idno></series><idno type="istex">2679B0934514CCBF4D3AA7FD1250AFDAA36E08F9</idno><idno type="DOI">10.1002/cne.1285</idno><idno type="ArticleID">CNE1285</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9967</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>GnRH</term><term>brain</term><term>fish</term><term>immunocytochemistry</term><term>ontogeny</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The early development of both the catfish gonadotropin‐releasing hormone (cfGnRH)‐ and the chicken GnRH‐II (cGnRH‐II) system was investigated in African catfish by immunocytochemistry by using antibodies against the GnRH‐associated peptide (GAP) of the respective preprohormones. Weakly cfGnRH‐immunoreactive (ir) neurons and fibers were present at 2 weeks after hatching (ph) but only in the ventral telencephalon and pituitary. Two weeks later, cfGnRH fibers and neurons were also observed in more rostral and in more caudal brain areas, mainly in the preoptic area and hypothalamus. Based on differences in temporal, spatial, and morphologic appearance, two distinct cfGnRH populations were identified in the ventral forebrain: a population innervating the pituitary (ventral forebrain system) and a so‐called terminal nerve (TN) population. DiI tracing studies revealed that the TN population has no neuronal connections with the pituitary. The cGnRH‐II system is present from 2 weeks ph onward in the midbrain tegmentum and only their size and staining intensity increased during development. Based on the comparison of GnRH systems amongst vertebrates, we hypothesize that during fish evolution, three different GnRH systems evolved, each expressing their own molecular form: the cGnRH‐II system in the midbrain, a hypophysiotropic GnRH system in the hypothalamus with a species‐specific GnRH form, and a salmon GnRH‐expressing TN population. This hypothesis is supported by phylogenetic analysis of known GnRH precursor amino acid sequences. We hypothesize, because the African catfish is a less advanced teleost species, that it contains the cfGnRH form both in the ventral forebrain system and in the TN population.J. Comp. Neurol. 437:308–320, 2001. © 2001 Wiley‐Liss, Inc.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Eline A. 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Weakly cfGnRH‐immunoreactive (ir) neurons and fibers were present at 2 weeks after hatching (ph) but only in the ventral telencephalon and pituitary. Two weeks later, cfGnRH fibers and neurons were also observed in more rostral and in more caudal brain areas, mainly in the preoptic area and hypothalamus. Based on differences in temporal, spatial, and morphologic appearance, two distinct cfGnRH populations were identified in the ventral forebrain: a population innervating the pituitary (ventral forebrain system) and a so‐called terminal nerve (TN) population. DiI tracing studies revealed that the TN population has no neuronal connections with the pituitary. The cGnRH‐II system is present from 2 weeks ph onward in the midbrain tegmentum and only their size and staining intensity increased during development. Based on the comparison of GnRH systems amongst vertebrates, we hypothesize that during fish evolution, three different GnRH systems evolved, each expressing their own molecular form: the cGnRH‐II system in the midbrain, a hypophysiotropic GnRH system in the hypothalamus with a species‐specific GnRH form, and a salmon GnRH‐expressing TN population. This hypothesis is supported by phylogenetic analysis of known GnRH precursor amino acid sequences. We hypothesize, because the African catfish is a less advanced teleost species, that it contains the cfGnRH form both in the ventral forebrain system and in the TN population.J. Comp. 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Weakly cfGnRH‐immunoreactive (ir) neurons and fibers were present at 2 weeks after hatching (ph) but only in the ventral telencephalon and pituitary. Two weeks later, cfGnRH fibers and neurons were also observed in more rostral and in more caudal brain areas, mainly in the preoptic area and hypothalamus. Based on differences in temporal, spatial, and morphologic appearance, two distinct cfGnRH populations were identified in the ventral forebrain: a population innervating the pituitary (ventral forebrain system) and a so‐called terminal nerve (TN) population. DiI tracing studies revealed that the TN population has no neuronal connections with the pituitary. The cGnRH‐II system is present from 2 weeks ph onward in the midbrain tegmentum and only their size and staining intensity increased during development. Based on the comparison of GnRH systems amongst vertebrates, we hypothesize that during fish evolution, three different GnRH systems evolved, each expressing their own molecular form: the cGnRH‐II system in the midbrain, a hypophysiotropic GnRH system in the hypothalamus with a species‐specific GnRH form, and a salmon GnRH‐expressing TN population. This hypothesis is supported by phylogenetic analysis of known GnRH precursor amino acid sequences. We hypothesize, because the African catfish is a less advanced teleost species, that it contains the cfGnRH form both in the ventral forebrain system and in the TN population.J. Comp. 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Th.</givenNames><familyName>Goos</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="NL" type="organization"><unparsedAffiliation>Research Group of Comparative Endocrinology, Graduate School for Developmental Biology, Faculty of Biology, 3584 CH Utrecht, The Netherlands</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">fish</keyword><keyword xml:id="kwd2">GnRH</keyword><keyword xml:id="kwd3">ontogeny</keyword><keyword xml:id="kwd4">immunocytochemistry</keyword><keyword xml:id="kwd5">brain</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>The early development of both the catfish gonadotropin‐releasing hormone (cfGnRH)‐ and the chicken GnRH‐II (cGnRH‐II) system was investigated in African catfish by immunocytochemistry by using antibodies against the GnRH‐associated peptide (GAP) of the respective preprohormones. Weakly cfGnRH‐immunoreactive (ir) neurons and fibers were present at 2 weeks after hatching (ph) but only in the ventral telencephalon and pituitary. Two weeks later, cfGnRH fibers and neurons were also observed in more rostral and in more caudal brain areas, mainly in the preoptic area and hypothalamus. Based on differences in temporal, spatial, and morphologic appearance, two distinct cfGnRH populations were identified in the ventral forebrain: a population innervating the pituitary (ventral forebrain system) and a so‐called terminal nerve (TN) population. DiI tracing studies revealed that the TN population has no neuronal connections with the pituitary. The cGnRH‐II system is present from 2 weeks ph onward in the midbrain tegmentum and only their size and staining intensity increased during development. Based on the comparison of GnRH systems amongst vertebrates, we hypothesize that during fish evolution, three different GnRH systems evolved, each expressing their own molecular form: the cGnRH‐II system in the midbrain, a hypophysiotropic GnRH system in the hypothalamus with a species‐specific GnRH form, and a salmon GnRH‐expressing TN population. This hypothesis is supported by phylogenetic analysis of known GnRH precursor amino acid sequences. We hypothesize, because the African catfish is a less advanced teleost species, that it contains the cfGnRH form both in the ventral forebrain system and in the TN population.J. Comp. Neurol. 437:308–320, 2001. © 2001 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Development of three distinct GnRH neuron populations expressing two different GnRH forms in the brain of the African catfish (Clarias gariepinus)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Development of Three GnRH Populations in Catfish Brain</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Development of three distinct GnRH neuron populations expressing two different GnRH forms in the brain of the African catfish (Clarias gariepinus)</title></titleInfo><name type="personal"><namePart type="given">Eline A.</namePart><namePart type="family">Dubois</namePart><affiliation>Research Group of Comparative Endocrinology, Graduate School for Developmental Biology, Faculty of Biology, 3584 CH Utrecht, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Matthijs A.</namePart><namePart type="family">Zandbergen</namePart><affiliation>Research Group of Comparative Endocrinology, Graduate School for Developmental Biology, Faculty of Biology, 3584 CH Utrecht, The Netherlands</affiliation><affiliation>Research Group of Comparative Endocrinology, Graduate School for Developmental Biology, Faculty of Biology, Padualaan 8, 3584 CH Utrecht, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jan</namePart><namePart type="family">Peute</namePart><affiliation>Research Group of Comparative Endocrinology, Graduate School for Developmental Biology, Faculty of Biology, 3584 CH Utrecht, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jan</namePart><namePart type="family">Bogerd</namePart><affiliation>Research Group of Comparative Endocrinology, Graduate School for Developmental Biology, Faculty of Biology, 3584 CH Utrecht, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Henk J. Th.</namePart><namePart type="family">Goos</namePart><affiliation>Research Group of Comparative Endocrinology, Graduate School for Developmental Biology, Faculty of Biology, 3584 CH Utrecht, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>John Wiley & Sons, Inc.</publisher><place><placeTerm type="text">New York</placeTerm></place><dateIssued encoding="w3cdtf">2001-08-27</dateIssued><dateCaptured encoding="w3cdtf">2000-12-19</dateCaptured><dateValid encoding="w3cdtf">2001-05-30</dateValid><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">6</extent><extent unit="tables">3</extent><extent unit="references">68</extent><extent unit="words">39467</extent></physicalDescription><abstract lang="en">The early development of both the catfish gonadotropin‐releasing hormone (cfGnRH)‐ and the chicken GnRH‐II (cGnRH‐II) system was investigated in African catfish by immunocytochemistry by using antibodies against the GnRH‐associated peptide (GAP) of the respective preprohormones. Weakly cfGnRH‐immunoreactive (ir) neurons and fibers were present at 2 weeks after hatching (ph) but only in the ventral telencephalon and pituitary. Two weeks later, cfGnRH fibers and neurons were also observed in more rostral and in more caudal brain areas, mainly in the preoptic area and hypothalamus. Based on differences in temporal, spatial, and morphologic appearance, two distinct cfGnRH populations were identified in the ventral forebrain: a population innervating the pituitary (ventral forebrain system) and a so‐called terminal nerve (TN) population. DiI tracing studies revealed that the TN population has no neuronal connections with the pituitary. The cGnRH‐II system is present from 2 weeks ph onward in the midbrain tegmentum and only their size and staining intensity increased during development. Based on the comparison of GnRH systems amongst vertebrates, we hypothesize that during fish evolution, three different GnRH systems evolved, each expressing their own molecular form: the cGnRH‐II system in the midbrain, a hypophysiotropic GnRH system in the hypothalamus with a species‐specific GnRH form, and a salmon GnRH‐expressing TN population. This hypothesis is supported by phylogenetic analysis of known GnRH precursor amino acid sequences. We hypothesize, because the African catfish is a less advanced teleost species, that it contains the cfGnRH form both in the ventral forebrain system and in the TN population.J. Comp. Neurol. 437:308–320, 2001. © 2001 Wiley‐Liss, Inc.</abstract><subject lang="en"><genre>keywords</genre><topic>fish</topic><topic>GnRH</topic><topic>ontogeny</topic><topic>immunocytochemistry</topic><topic>brain</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Comparative Neurology</title></titleInfo><titleInfo type="abbreviated"><title>J. Comp. Neurol.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic></subject><identifier type="ISSN">0021-9967</identifier><identifier type="eISSN">1096-9861</identifier><identifier type="DOI">10.1002/(ISSN)1096-9861</identifier><identifier type="PublisherID">CNE</identifier><part><date>2001</date><detail type="volume"><caption>vol.</caption><number>437</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>308</start><end>320</end><total>13</total></extent></part></relatedItem><identifier type="istex">2679B0934514CCBF4D3AA7FD1250AFDAA36E08F9</identifier><identifier type="DOI">10.1002/cne.1285</identifier><identifier type="ArticleID">CNE1285</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2001 Wiley‐Liss, Inc.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>John Wiley & Sons, Inc.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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