Comparative immunocytochemical study of FMRFamide neuronal system in the brain of Danio rerio and Acipenser ruthenus during development
Identifieur interne : 001348 ( Istex/Corpus ); précédent : 001347; suivant : 001349Comparative immunocytochemical study of FMRFamide neuronal system in the brain of Danio rerio and Acipenser ruthenus during development
Auteurs : Claudia Pinelli ; Biagio D'Aniello ; Paolo Sordino ; Dietrich L. Meyer ; Maria Fiorentino ; Rakesh K. RastogiSource :
- Developmental Brain Research [ 0165-3806 ] ; 2000.
Abstract
The distribution of FMRFamide-like immunoreactive (ir) neurons and fibers was investigated in the central nervous system of developing zebrafish and juvenile sturgeon (sterlet). Adult zebrafish was also studied. In zebrafish embryos FMRFamide-ir elements first appeared 30 h post-fertilization (PF). Ir somata were located in the olfactory placode and in the ventral diencephalon. FMRFamide-ir fibers originating from diencephalic neurons were found in the ventral telencephalon and in ventral portions of the brainstem. At 48 h PF, the ir perikarya in the olfactory placode displayed increased immunoreactivity and stained fibers emerged from the somata. At 60 h PF, bilaterally, clusters of FMRFamide-ir neurons were found along the rostro-caudal axis of the brain, from the olfactory placode to rostral regions of the ventro-lateral telencephalon. At 60 h PF, numerous ir fibers appeared in the dorsal telencephalon, optic lobes, optic nerves, and retina. Except for ir fibers in the hypophysis at the age of 72 h PF, and a few ir cells in the nucleus olfacto-retinalis (NOR) at the age of 2 months PF, no major re-organization was noted in subsequent ontogenetic stages. The number of stained NOR neurons increased markedly in sexually mature zebrafish. In adult zebrafish, other ir neurons were located in the dorsal zones of the periventricular hypothalamus and in components of the nervus terminalis. We are inclined to believe that neurons expressing FMRFamide originate in the olfactory placode and in the ventricular ependyma in the hypothalamus. On the same grounds, a dual origin of FMRFamide-ir neurons is inferred in the sturgeon, an ancestral bony fish: prior to the observation of ir cells in the nasal area and in the telencephalon stained neurons were noted in circumventricular hypothalamic regions.
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DOI: 10.1016/S0165-3806(99)00172-8
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Meyer</name><affiliation><mods:affiliation>Department of Neuroanatomy, School of Medicine, University of Göttingen, 37075 Göttingen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Fiorentino, Maria" sort="Fiorentino, Maria" uniqKey="Fiorentino M" first="Maria" last="Fiorentino">Maria Fiorentino</name><affiliation><mods:affiliation>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</mods:affiliation></affiliation></author><author><name sortKey="Rastogi, Rakesh K" sort="Rastogi, Rakesh K" uniqKey="Rastogi R" first="Rakesh K" last="Rastogi">Rakesh K. 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Meyer</name><affiliation><mods:affiliation>Department of Neuroanatomy, School of Medicine, University of Göttingen, 37075 Göttingen, Germany</mods:affiliation></affiliation></author><author><name sortKey="Fiorentino, Maria" sort="Fiorentino, Maria" uniqKey="Fiorentino M" first="Maria" last="Fiorentino">Maria Fiorentino</name><affiliation><mods:affiliation>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</mods:affiliation></affiliation></author><author><name sortKey="Rastogi, Rakesh K" sort="Rastogi, Rakesh K" uniqKey="Rastogi R" first="Rakesh K" last="Rastogi">Rakesh K. Rastogi</name><affiliation><mods:affiliation>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Developmental Brain Research</title><title level="j" type="abbrev">BRESD</title><idno type="ISSN">0165-3806</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">119</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="195">195</biblScope><biblScope unit="page" to="208">208</biblScope></imprint><idno type="ISSN">0165-3806</idno></series><idno type="istex">E362D10A4AD5C431D2AD2A499A55668455C0D323</idno><idno type="DOI">10.1016/S0165-3806(99)00172-8</idno><idno type="PII">S0165-3806(99)00172-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0165-3806</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The distribution of FMRFamide-like immunoreactive (ir) neurons and fibers was investigated in the central nervous system of developing zebrafish and juvenile sturgeon (sterlet). Adult zebrafish was also studied. In zebrafish embryos FMRFamide-ir elements first appeared 30 h post-fertilization (PF). Ir somata were located in the olfactory placode and in the ventral diencephalon. FMRFamide-ir fibers originating from diencephalic neurons were found in the ventral telencephalon and in ventral portions of the brainstem. At 48 h PF, the ir perikarya in the olfactory placode displayed increased immunoreactivity and stained fibers emerged from the somata. At 60 h PF, bilaterally, clusters of FMRFamide-ir neurons were found along the rostro-caudal axis of the brain, from the olfactory placode to rostral regions of the ventro-lateral telencephalon. At 60 h PF, numerous ir fibers appeared in the dorsal telencephalon, optic lobes, optic nerves, and retina. Except for ir fibers in the hypophysis at the age of 72 h PF, and a few ir cells in the nucleus olfacto-retinalis (NOR) at the age of 2 months PF, no major re-organization was noted in subsequent ontogenetic stages. The number of stained NOR neurons increased markedly in sexually mature zebrafish. In adult zebrafish, other ir neurons were located in the dorsal zones of the periventricular hypothalamus and in components of the nervus terminalis. We are inclined to believe that neurons expressing FMRFamide originate in the olfactory placode and in the ventricular ependyma in the hypothalamus. On the same grounds, a dual origin of FMRFamide-ir neurons is inferred in the sturgeon, an ancestral bony fish: prior to the observation of ir cells in the nasal area and in the telencephalon stained neurons were noted in circumventricular hypothalamic regions.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Claudia Pinelli</name><affiliations><json:string>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</json:string></affiliations></json:item><json:item><name>Biagio D'Aniello</name><affiliations><json:string>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</json:string></affiliations></json:item><json:item><name>Paolo Sordino</name><affiliations><json:string>Department of Anatomy and Developmental Biology, University College London, Gower Street, London, WC1E 6BT, UK</json:string></affiliations></json:item><json:item><name>Dietrich L Meyer</name><affiliations><json:string>Department of Neuroanatomy, School of Medicine, University of Göttingen, 37075 Göttingen, Germany</json:string></affiliations></json:item><json:item><name>Maria Fiorentino</name><affiliations><json:string>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</json:string></affiliations></json:item><json:item><name>Rakesh K Rastogi</name><affiliations><json:string>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>FMRFamide</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Zebrafish</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Sterlet</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Ontogeny</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Immunohistochemistry</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>The distribution of FMRFamide-like immunoreactive (ir) neurons and fibers was investigated in the central nervous system of developing zebrafish and juvenile sturgeon (sterlet). Adult zebrafish was also studied. In zebrafish embryos FMRFamide-ir elements first appeared 30 h post-fertilization (PF). Ir somata were located in the olfactory placode and in the ventral diencephalon. FMRFamide-ir fibers originating from diencephalic neurons were found in the ventral telencephalon and in ventral portions of the brainstem. At 48 h PF, the ir perikarya in the olfactory placode displayed increased immunoreactivity and stained fibers emerged from the somata. At 60 h PF, bilaterally, clusters of FMRFamide-ir neurons were found along the rostro-caudal axis of the brain, from the olfactory placode to rostral regions of the ventro-lateral telencephalon. At 60 h PF, numerous ir fibers appeared in the dorsal telencephalon, optic lobes, optic nerves, and retina. Except for ir fibers in the hypophysis at the age of 72 h PF, and a few ir cells in the nucleus olfacto-retinalis (NOR) at the age of 2 months PF, no major re-organization was noted in subsequent ontogenetic stages. The number of stained NOR neurons increased markedly in sexually mature zebrafish. In adult zebrafish, other ir neurons were located in the dorsal zones of the periventricular hypothalamus and in components of the nervus terminalis. We are inclined to believe that neurons expressing FMRFamide originate in the olfactory placode and in the ventricular ependyma in the hypothalamus. On the same grounds, a dual origin of FMRFamide-ir neurons is inferred in the sturgeon, an ancestral bony fish: prior to the observation of ir cells in the nasal area and in the telencephalon stained neurons were noted in circumventricular hypothalamic regions.</abstract><qualityIndicators><score>8</score><pdfVersion>1.2</pdfVersion><pdfPageSize>595 x 758 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1818</abstractCharCount><pdfWordCount>7200</pdfWordCount><pdfCharCount>45672</pdfCharCount><pdfPageCount>14</pdfPageCount><abstractWordCount>278</abstractWordCount></qualityIndicators><title>Comparative immunocytochemical study of FMRFamide neuronal system in the brain of Danio rerio and Acipenser ruthenus during development</title><pii><json:string>S0165-3806(99)00172-8</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>119</volume><pii><json:string>S0165-3806(00)X0057-0</json:string></pii><pages><last>208</last><first>195</first></pages><issn><json:string>0165-3806</json:string></issn><issue>2</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Developmental Brain Research</title><publicationDate>2000</publicationDate></host><categories><wos></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>neurology & neurosurgery</json:string></scienceMetrix></categories><publicationDate>2000</publicationDate><copyrightDate>2000</copyrightDate><doi><json:string>10.1016/S0165-3806(99)00172-8</json:string></doi><id>E362D10A4AD5C431D2AD2A499A55668455C0D323</id><score>0.04445307</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/E362D10A4AD5C431D2AD2A499A55668455C0D323/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/E362D10A4AD5C431D2AD2A499A55668455C0D323/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/E362D10A4AD5C431D2AD2A499A55668455C0D323/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Comparative immunocytochemical study of FMRFamide neuronal system in the brain of Danio rerio and Acipenser ruthenus during development</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>2000</date></publicationStmt><notesStmt><note type="content">Section title: Research report</note><note type="content">Fig. 1: Schematic line drawings illustrating the distribution of FMRFamide-ir neurons (large dots) and fibers (small dots) in sagittal sections of the zebrafish brain. CBL, cerebellum; DIE, diencephalon; DT, dorsal telencephalon; NOR, nucleus olfacto-retinalis; OB, olfactory bulb; OC, optic chiasma; OP, olfactory placode; OT, optic tectum; PH, periventricular hypothalamic nucleus; RHOM, rhombencephalon; TEL, telencephalon; TN, terminal nerve; VH, ventral hypothalamus; VLT, ventral longitudinal tract.</note><note type="content">Fig. 2: (A–B) Sagittal sections of a 48-h PF embryo visualizing FMRFamide-ir neurons (heavy arrows) in olfactory placode (A) and diencephalon (B). (C–D) Transverse sections of a 60-h PF embryo showing FMRFamide-ir neurons (heavy arrows) and fibers in the area where the olfactory placode contacts the forebrain (C) as well as in the diencephalon (D). Inserts give low power photomicrographs of sections; light arrows depict regions given at high magnifications. DIE, diencephalon; OP, olfactory placode; TEL, telencephalon. Scale bar=50 μm.</note><note type="content">Fig. 3: Wholemount immunostaining of FMRFamide-ir pathways in the brain of a 60-h PF embryo. Heavy arrows indicate the nasal (left) and diencephalic (right) groups of FMRFamide-ir neurons. Light arrows depict four major fiber tracts originating from the diencephalic cluster of ir somata. Scale bar=1 mm.</note><note type="content">Fig. 4: (A) FMRFamide-ir fibers (heavy arrows) in the eye of a 60-h PF embryo, transverse section. Asterisk optic nerve. (B) FMRFamide immunoreactivity in the pituitary (heavy arrow) of a 72-h PF embryo. (C) Transverse section of a 5-day PF fry revealing FMRFamide-ir neurons (heavy arrows) in the ventral hypothalamus. Inserts give low power photomicrographs of sections; light arrows depict regions given at high magnifications. PIT, pituitary. Scale bar=50 μm.</note><note type="content">Fig. 5: (A) Horizontal section of the brain of one month old fry showing several FMRFamide-ir neurons (heavy arrows) located along the nervus terminalis that runs adjacent to the olfactory bulb. (B) Sagittal section of the brain of a 2-month-old fry revealing FMRFamide-ir neurons (heavy arrows) in the terminal nerve ventral to the olfactory bulb. (C) Sagittal section of same brain as in B showing the NOR containing a few FMRFamide-ir neurons (heavy arrow) with long fibers fanning out caudally into the telencephalon. Inserts give low power photomicrographs of sections; light arrows depict regions given at high magnifications. OB, olfactory bulb. Scale bar=50 μm.</note><note type="content">Fig. 6: (A) Sagittal section of an adult zebrafish brain showing FMRFamide-ir neurons (heavy arrows) in the terminal nerve ventral to the olfactory bulb. (B) Same brain as in A, showing that most neurons of the NOR are labeled by anti-FMRFamide (heavy arrow). (C) Transverse section of an adult brain showing two clusters of diencephalic FMRFamide-ir neurons (heavy arrows). Inserts give low power photomicrographs of sections; light arrows depict regions given at high magnifications. Scale bar=50 μm.</note><note type="content">Fig. 7: Schematic line drawings illustrating the distribution of FMRFamide-ir neurons (large dots) and fibers (small dots) in transverse sections of the developing sterlet brain. Heavy arrows point to the corresponding areas photographed and shown in the next figure. E, eye; Hd, dorsal hypothalamus; Hv, ventral hypothalamus; MES, mesencephalon; OM, olfactory mucosa; Tc, caudal telencephalon; Tm, midtelencephalon; Tr, rostral telencephalon.</note><note type="content">Fig. 8: (A) Transverse section of the head of a 14-mm sterlet showing a group of FMRFamide neurons (heavy arrow) outside the brain and close to the olfactory mucosa (OM). Rostral telencephalon is to the right. The area is pointed by an arrow in Fig. 7A. (B) Localization of FMRFamide-ir neurons (heavy arrow) at the level of rostral telencephalon of a 15-mm sterlet, as indicated by arrow in Fig. 7A. (C) FMRFamide neurons in the midventral caudal telencephalon of a 15-mm sterlet, as shown by arrow in Fig. 7C. (D) FMRFamide neurons in the ventral hypothalamus as indicated by arrow in Fig. 7E. Most neurons have cerebrospinal fluid-contacting fiber projections. Scale bar=50 μm.</note><note type="content">Fig. 9: Schematic line drawings illustrating the distribution of FMRFamide-ir neurons (large dots) and fibers (fine dots) in representative transverse sections of a 21.5-cm sterlet brain. D, dorsal telencephalic area; Dd, dorsal zone of dorsal telencephalic area; Dl, lateral zone of dorsal telencephalic area; Dp posterior zone of dorsal telencephalic area; EN, entopeduncolar nucleus; GL, glomerular layer of the olfactory bulb; H, habenule; MOT, medial olfactory tract; NMLF, nucleus of medial longitudinal fascicle; OC, optic chiasm; OT, optic tectum/tectum mesencephali; Pc, periventricular hypothalamus, caudal part; Pd, periventricular hypothalamus, dorsal part; Pl, periventricular hypothalamus, lateral part; PM, magnocellular preoptic nucleus; PP, parvocellular preoptic nucleus; Pv, periventricular hypothalamus, ventral part; SC, suprachiasmatic nucleus; Thd, dorsal thalamus; Thv, ventral thalamus; TL, torus longitudinalis; Tla, torus lateralis; TPp, periventricular nucleus of posterior tuberculum; Va, valvula cerebelli; Vd, dorsal nucleus of the ventral telencephalic area.</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Comparative immunocytochemical study of FMRFamide neuronal system in the brain of Danio rerio and Acipenser ruthenus during development</title><author xml:id="author-1"><persName><forename type="first">Claudia</forename><surname>Pinelli</surname></persName><affiliation>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</affiliation></author><author xml:id="author-2"><persName><forename type="first">Biagio</forename><surname>D'Aniello</surname></persName><affiliation>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</affiliation></author><author xml:id="author-3"><persName><forename type="first">Paolo</forename><surname>Sordino</surname></persName><affiliation>Department of Anatomy and Developmental Biology, University College London, Gower Street, London, WC1E 6BT, UK</affiliation></author><author xml:id="author-4"><persName><forename type="first">Dietrich L</forename><surname>Meyer</surname></persName><note type="biography">Deceased on 8/6/99.</note><affiliation>Deceased on 8/6/99.</affiliation><affiliation>Department of Neuroanatomy, School of Medicine, University of Göttingen, 37075 Göttingen, Germany</affiliation></author><author xml:id="author-5"><persName><forename type="first">Maria</forename><surname>Fiorentino</surname></persName><affiliation>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</affiliation></author><author xml:id="author-6"><persName><forename type="first">Rakesh K</forename><surname>Rastogi</surname></persName><note type="correspondence"><p>Corresponding author. Fax: +0039-081-7903342; e-mail: rastogi@unina.it</p></note><affiliation>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</affiliation></author></analytic><monogr><title level="j">Developmental Brain Research</title><title level="j" type="abbrev">BRESD</title><idno type="pISSN">0165-3806</idno><idno type="PII">S0165-3806(00)X0057-0</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000"></date><biblScope unit="volume">119</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="195">195</biblScope><biblScope unit="page" to="208">208</biblScope></imprint></monogr><idno type="istex">E362D10A4AD5C431D2AD2A499A55668455C0D323</idno><idno type="DOI">10.1016/S0165-3806(99)00172-8</idno><idno type="PII">S0165-3806(99)00172-8</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2000</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The distribution of FMRFamide-like immunoreactive (ir) neurons and fibers was investigated in the central nervous system of developing zebrafish and juvenile sturgeon (sterlet). Adult zebrafish was also studied. In zebrafish embryos FMRFamide-ir elements first appeared 30 h post-fertilization (PF). Ir somata were located in the olfactory placode and in the ventral diencephalon. FMRFamide-ir fibers originating from diencephalic neurons were found in the ventral telencephalon and in ventral portions of the brainstem. At 48 h PF, the ir perikarya in the olfactory placode displayed increased immunoreactivity and stained fibers emerged from the somata. At 60 h PF, bilaterally, clusters of FMRFamide-ir neurons were found along the rostro-caudal axis of the brain, from the olfactory placode to rostral regions of the ventro-lateral telencephalon. At 60 h PF, numerous ir fibers appeared in the dorsal telencephalon, optic lobes, optic nerves, and retina. Except for ir fibers in the hypophysis at the age of 72 h PF, and a few ir cells in the nucleus olfacto-retinalis (NOR) at the age of 2 months PF, no major re-organization was noted in subsequent ontogenetic stages. The number of stained NOR neurons increased markedly in sexually mature zebrafish. In adult zebrafish, other ir neurons were located in the dorsal zones of the periventricular hypothalamus and in components of the nervus terminalis. We are inclined to believe that neurons expressing FMRFamide originate in the olfactory placode and in the ventricular ependyma in the hypothalamus. On the same grounds, a dual origin of FMRFamide-ir neurons is inferred in the sturgeon, an ancestral bony fish: prior to the observation of ir cells in the nasal area and in the telencephalon stained neurons were noted in circumventricular hypothalamic regions.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>FMRFamide</term></item><item><term>Zebrafish</term></item><item><term>Sterlet</term></item><item><term>Ontogeny</term></item><item><term>Immunohistochemistry</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2000">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/E362D10A4AD5C431D2AD2A499A55668455C0D323/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity><istex:entity SYSTEM="gr4" NDATA="IMAGE" name="gr4"></istex:entity><istex:entity SYSTEM="gr5" NDATA="IMAGE" name="gr5"></istex:entity><istex:entity SYSTEM="gr6" NDATA="IMAGE" name="gr6"></istex:entity><istex:entity SYSTEM="gr7" NDATA="IMAGE" name="gr7"></istex:entity><istex:entity SYSTEM="gr8" NDATA="IMAGE" name="gr8"></istex:entity><istex:entity SYSTEM="gr9" NDATA="IMAGE" name="gr9"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>BRESD</jid><aid>52768</aid><ce:pii>S0165-3806(99)00172-8</ce:pii><ce:doi>10.1016/S0165-3806(99)00172-8</ce:doi><ce:copyright year="2000" type="unknown"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Research report</ce:textfn></ce:dochead><ce:title>Comparative immunocytochemical study of FMRFamide neuronal system in the brain of <ce:italic>Danio rerio</ce:italic> and <ce:italic>Acipenser ruthenus</ce:italic> during development</ce:title><ce:author-group><ce:author><ce:given-name>Claudia</ce:given-name><ce:surname>Pinelli</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref></ce:author><ce:author><ce:given-name>Biagio</ce:given-name><ce:surname>D'Aniello</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref></ce:author><ce:author><ce:given-name>Paolo</ce:given-name><ce:surname>Sordino</ce:surname><ce:cross-ref refid="AFF2">b</ce:cross-ref></ce:author><ce:author><ce:given-name>Dietrich L</ce:given-name><ce:surname>Meyer</ce:surname><ce:cross-ref refid="AFF3">c</ce:cross-ref><ce:cross-ref refid="FN1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Maria</ce:given-name><ce:surname>Fiorentino</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref></ce:author><ce:author><ce:given-name>Rakesh K</ce:given-name><ce:surname>Rastogi</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Department of Anatomy and Developmental Biology, University College London, Gower Street, London, WC1E 6BT, UK</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Department of Neuroanatomy, School of Medicine, University of Göttingen, 37075 Göttingen, Germany</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Fax: +0039-081-7903342; e-mail: rastogi@unina.it</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>1</ce:label><ce:note-para>Deceased on 8/6/99.</ce:note-para></ce:footnote></ce:author-group><ce:date-accepted day="2" month="11" year="1999"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The distribution of FMRFamide-like immunoreactive (ir) neurons and fibers was investigated in the central nervous system of developing zebrafish and juvenile sturgeon (sterlet). Adult zebrafish was also studied. In zebrafish embryos FMRFamide-ir elements first appeared 30 h post-fertilization (PF). Ir somata were located in the olfactory placode and in the ventral diencephalon. FMRFamide-ir fibers originating from diencephalic neurons were found in the ventral telencephalon and in ventral portions of the brainstem. At 48 h PF, the ir perikarya in the olfactory placode displayed increased immunoreactivity and stained fibers emerged from the somata. At 60 h PF, bilaterally, clusters of FMRFamide-ir neurons were found along the rostro-caudal axis of the brain, from the olfactory placode to rostral regions of the ventro-lateral telencephalon. At 60 h PF, numerous ir fibers appeared in the dorsal telencephalon, optic lobes, optic nerves, and retina. Except for ir fibers in the hypophysis at the age of 72 h PF, and a few ir cells in the nucleus olfacto-retinalis (NOR) at the age of 2 months PF, no major re-organization was noted in subsequent ontogenetic stages. The number of stained NOR neurons increased markedly in sexually mature zebrafish. In adult zebrafish, other ir neurons were located in the dorsal zones of the periventricular hypothalamus and in components of the nervus terminalis. We are inclined to believe that neurons expressing FMRFamide originate in the olfactory placode and in the ventricular ependyma in the hypothalamus. On the same grounds, a dual origin of FMRFamide-ir neurons is inferred in the sturgeon, an ancestral bony fish: prior to the observation of ir cells in the nasal area and in the telencephalon stained neurons were noted in circumventricular hypothalamic regions.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>FMRFamide</ce:text></ce:keyword><ce:keyword><ce:text>Zebrafish</ce:text></ce:keyword><ce:keyword><ce:text>Sterlet</ce:text></ce:keyword><ce:keyword><ce:text>Ontogeny</ce:text></ce:keyword><ce:keyword><ce:text>Immunohistochemistry</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Comparative immunocytochemical study of FMRFamide neuronal system in the brain of Danio rerio and Acipenser ruthenus during development</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Comparative immunocytochemical study of FMRFamide neuronal system in the brain of</title></titleInfo><name type="personal"><namePart type="given">Claudia</namePart><namePart type="family">Pinelli</namePart><affiliation>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Biagio</namePart><namePart type="family">D'Aniello</namePart><affiliation>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Paolo</namePart><namePart type="family">Sordino</namePart><affiliation>Department of Anatomy and Developmental Biology, University College London, Gower Street, London, WC1E 6BT, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dietrich L</namePart><namePart type="family">Meyer</namePart><affiliation>Department of Neuroanatomy, School of Medicine, University of Göttingen, 37075 Göttingen, Germany</affiliation><description>Deceased on 8/6/99.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Maria</namePart><namePart type="family">Fiorentino</namePart><affiliation>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Rakesh K</namePart><namePart type="family">Rastogi</namePart><affiliation>Dipartimento di Zoologia, Università di Napoli Frederico II, Via Mezzocannone 8, 80134 Naples, Italy</affiliation><description>Corresponding author. Fax: +0039-081-7903342; e-mail: rastogi@unina.it</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2000</dateIssued><copyrightDate encoding="w3cdtf">2000</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The distribution of FMRFamide-like immunoreactive (ir) neurons and fibers was investigated in the central nervous system of developing zebrafish and juvenile sturgeon (sterlet). Adult zebrafish was also studied. In zebrafish embryos FMRFamide-ir elements first appeared 30 h post-fertilization (PF). Ir somata were located in the olfactory placode and in the ventral diencephalon. FMRFamide-ir fibers originating from diencephalic neurons were found in the ventral telencephalon and in ventral portions of the brainstem. At 48 h PF, the ir perikarya in the olfactory placode displayed increased immunoreactivity and stained fibers emerged from the somata. At 60 h PF, bilaterally, clusters of FMRFamide-ir neurons were found along the rostro-caudal axis of the brain, from the olfactory placode to rostral regions of the ventro-lateral telencephalon. At 60 h PF, numerous ir fibers appeared in the dorsal telencephalon, optic lobes, optic nerves, and retina. Except for ir fibers in the hypophysis at the age of 72 h PF, and a few ir cells in the nucleus olfacto-retinalis (NOR) at the age of 2 months PF, no major re-organization was noted in subsequent ontogenetic stages. The number of stained NOR neurons increased markedly in sexually mature zebrafish. In adult zebrafish, other ir neurons were located in the dorsal zones of the periventricular hypothalamus and in components of the nervus terminalis. We are inclined to believe that neurons expressing FMRFamide originate in the olfactory placode and in the ventricular ependyma in the hypothalamus. On the same grounds, a dual origin of FMRFamide-ir neurons is inferred in the sturgeon, an ancestral bony fish: prior to the observation of ir cells in the nasal area and in the telencephalon stained neurons were noted in circumventricular hypothalamic regions.</abstract><note type="content">Section title: Research report</note><note type="content">Fig. 1: Schematic line drawings illustrating the distribution of FMRFamide-ir neurons (large dots) and fibers (small dots) in sagittal sections of the zebrafish brain. CBL, cerebellum; DIE, diencephalon; DT, dorsal telencephalon; NOR, nucleus olfacto-retinalis; OB, olfactory bulb; OC, optic chiasma; OP, olfactory placode; OT, optic tectum; PH, periventricular hypothalamic nucleus; RHOM, rhombencephalon; TEL, telencephalon; TN, terminal nerve; VH, ventral hypothalamus; VLT, ventral longitudinal tract.</note><note type="content">Fig. 2: (A–B) Sagittal sections of a 48-h PF embryo visualizing FMRFamide-ir neurons (heavy arrows) in olfactory placode (A) and diencephalon (B). (C–D) Transverse sections of a 60-h PF embryo showing FMRFamide-ir neurons (heavy arrows) and fibers in the area where the olfactory placode contacts the forebrain (C) as well as in the diencephalon (D). Inserts give low power photomicrographs of sections; light arrows depict regions given at high magnifications. DIE, diencephalon; OP, olfactory placode; TEL, telencephalon. Scale bar=50 μm.</note><note type="content">Fig. 3: Wholemount immunostaining of FMRFamide-ir pathways in the brain of a 60-h PF embryo. Heavy arrows indicate the nasal (left) and diencephalic (right) groups of FMRFamide-ir neurons. Light arrows depict four major fiber tracts originating from the diencephalic cluster of ir somata. Scale bar=1 mm.</note><note type="content">Fig. 4: (A) FMRFamide-ir fibers (heavy arrows) in the eye of a 60-h PF embryo, transverse section. Asterisk optic nerve. (B) FMRFamide immunoreactivity in the pituitary (heavy arrow) of a 72-h PF embryo. (C) Transverse section of a 5-day PF fry revealing FMRFamide-ir neurons (heavy arrows) in the ventral hypothalamus. Inserts give low power photomicrographs of sections; light arrows depict regions given at high magnifications. PIT, pituitary. Scale bar=50 μm.</note><note type="content">Fig. 5: (A) Horizontal section of the brain of one month old fry showing several FMRFamide-ir neurons (heavy arrows) located along the nervus terminalis that runs adjacent to the olfactory bulb. (B) Sagittal section of the brain of a 2-month-old fry revealing FMRFamide-ir neurons (heavy arrows) in the terminal nerve ventral to the olfactory bulb. (C) Sagittal section of same brain as in B showing the NOR containing a few FMRFamide-ir neurons (heavy arrow) with long fibers fanning out caudally into the telencephalon. Inserts give low power photomicrographs of sections; light arrows depict regions given at high magnifications. OB, olfactory bulb. Scale bar=50 μm.</note><note type="content">Fig. 6: (A) Sagittal section of an adult zebrafish brain showing FMRFamide-ir neurons (heavy arrows) in the terminal nerve ventral to the olfactory bulb. (B) Same brain as in A, showing that most neurons of the NOR are labeled by anti-FMRFamide (heavy arrow). (C) Transverse section of an adult brain showing two clusters of diencephalic FMRFamide-ir neurons (heavy arrows). Inserts give low power photomicrographs of sections; light arrows depict regions given at high magnifications. Scale bar=50 μm.</note><note type="content">Fig. 7: Schematic line drawings illustrating the distribution of FMRFamide-ir neurons (large dots) and fibers (small dots) in transverse sections of the developing sterlet brain. Heavy arrows point to the corresponding areas photographed and shown in the next figure. E, eye; Hd, dorsal hypothalamus; Hv, ventral hypothalamus; MES, mesencephalon; OM, olfactory mucosa; Tc, caudal telencephalon; Tm, midtelencephalon; Tr, rostral telencephalon.</note><note type="content">Fig. 8: (A) Transverse section of the head of a 14-mm sterlet showing a group of FMRFamide neurons (heavy arrow) outside the brain and close to the olfactory mucosa (OM). Rostral telencephalon is to the right. The area is pointed by an arrow in Fig. 7A. (B) Localization of FMRFamide-ir neurons (heavy arrow) at the level of rostral telencephalon of a 15-mm sterlet, as indicated by arrow in Fig. 7A. (C) FMRFamide neurons in the midventral caudal telencephalon of a 15-mm sterlet, as shown by arrow in Fig. 7C. (D) FMRFamide neurons in the ventral hypothalamus as indicated by arrow in Fig. 7E. Most neurons have cerebrospinal fluid-contacting fiber projections. Scale bar=50 μm.</note><note type="content">Fig. 9: Schematic line drawings illustrating the distribution of FMRFamide-ir neurons (large dots) and fibers (fine dots) in representative transverse sections of a 21.5-cm sterlet brain. D, dorsal telencephalic area; Dd, dorsal zone of dorsal telencephalic area; Dl, lateral zone of dorsal telencephalic area; Dp posterior zone of dorsal telencephalic area; EN, entopeduncolar nucleus; GL, glomerular layer of the olfactory bulb; H, habenule; MOT, medial olfactory tract; NMLF, nucleus of medial longitudinal fascicle; OC, optic chiasm; OT, optic tectum/tectum mesencephali; Pc, periventricular hypothalamus, caudal part; Pd, periventricular hypothalamus, dorsal part; Pl, periventricular hypothalamus, lateral part; PM, magnocellular preoptic nucleus; PP, parvocellular preoptic nucleus; Pv, periventricular hypothalamus, ventral part; SC, suprachiasmatic nucleus; Thd, dorsal thalamus; Thv, ventral thalamus; TL, torus longitudinalis; Tla, torus lateralis; TPp, periventricular nucleus of posterior tuberculum; Va, valvula cerebelli; Vd, dorsal nucleus of the ventral telencephalic area.</note><subject><genre>Keywords</genre><topic>FMRFamide</topic><topic>Zebrafish</topic><topic>Sterlet</topic><topic>Ontogeny</topic><topic>Immunohistochemistry</topic></subject><relatedItem type="host"><titleInfo><title>Developmental Brain Research</title></titleInfo><titleInfo type="abbreviated"><title>BRESD</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">20000207</dateIssued></originInfo><identifier type="ISSN">0165-3806</identifier><identifier type="PII">S0165-3806(00)X0057-0</identifier><part><date>20000207</date><detail type="volume"><number>119</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue pages"><start>159</start><end>322</end></extent><extent unit="pages"><start>195</start><end>208</end></extent></part></relatedItem><identifier type="istex">E362D10A4AD5C431D2AD2A499A55668455C0D323</identifier><identifier type="DOI">10.1016/S0165-3806(99)00172-8</identifier><identifier type="PII">S0165-3806(99)00172-8</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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