Study of the spinal cords of the sturgeon Acipenser schrenckii, gar Lepisosteus oculatus, and goldfish Carassius auratus by morphological, immunohistochemical, and biochemical approaches
Identifieur interne : 001347 ( Istex/Corpus ); précédent : 001346; suivant : 001348Study of the spinal cords of the sturgeon Acipenser schrenckii, gar Lepisosteus oculatus, and goldfish Carassius auratus by morphological, immunohistochemical, and biochemical approaches
Auteurs : Maria Sen Mun Wai ; Dietrich Ernst Lorke ; Aiqun Zhang ; Hsiang-Fu Kung ; David T. YewSource :
- Microscopy Research and Technique [ 1059-910X ] ; 2007-12.
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- KwdEn :
Abstract
Little is known about the spinal cords of phylogenetically ancient actinopterygeans. The spinal cords of the chondrostean Acipenser schrenckii (Amur sturgeon), holostean Lepisosteus oculatus (spotted gar), and teleost Carassius auratus (goldfish) were, therefore, analyzed by immunohistochemistry, electron microscopy and two‐dimensional gel electrophoresis. Morphology showed numerous similarities between sturgeons and gars. In both, a dorsal column between the two dorsal horns was lacking, giving the grey matter an inverted Y‐shape. In goldfish, a small dorsal column was seen, the grey matter occupied a larger area, neuronal density was much higher, and a ventral commissure was apparent, which was absent in sturgeons and gars. In the white matter of sturgeons and gars, small caliber axons predominated, whereas larger axons were frequent in goldfish. Choline acetyltransferase immunoreactive neurons were prevalent in the ventral horns of all three fish, mainly in motoneurons, but stained fibers were only found in sturgeons and gars. γ‐aminobutyric acid positive cells were seen in both the ventral and the dorsal horns of all three fish. Distribution of serotonin (5‐HT) and tyrosine hydroxylase (TH) immunoreaction was similar in sturgeons and gars, being located in both the ventral and the dorsal horns. In goldfish, 5‐HT label was confined to the ventral horn and TH label was mainly observed in a cell group located ventromedially. Two‐dimensional gel electrophoresis showed a gradual increase in protein number from sturgeons to gars to goldfish. In conclusion, the spinal cords of sturgeons and gars share many morphological and chemical features, distinguishing them from the goldfish spinal cord. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.
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DOI: 10.1002/jemt.20515
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The spinal cords of the chondrostean Acipenser schrenckii (Amur sturgeon), holostean Lepisosteus oculatus (spotted gar), and teleost Carassius auratus (goldfish) were, therefore, analyzed by immunohistochemistry, electron microscopy and two‐dimensional gel electrophoresis. Morphology showed numerous similarities between sturgeons and gars. In both, a dorsal column between the two dorsal horns was lacking, giving the grey matter an inverted Y‐shape. In goldfish, a small dorsal column was seen, the grey matter occupied a larger area, neuronal density was much higher, and a ventral commissure was apparent, which was absent in sturgeons and gars. In the white matter of sturgeons and gars, small caliber axons predominated, whereas larger axons were frequent in goldfish. Choline acetyltransferase immunoreactive neurons were prevalent in the ventral horns of all three fish, mainly in motoneurons, but stained fibers were only found in sturgeons and gars. γ‐aminobutyric acid positive cells were seen in both the ventral and the dorsal horns of all three fish. Distribution of serotonin (5‐HT) and tyrosine hydroxylase (TH) immunoreaction was similar in sturgeons and gars, being located in both the ventral and the dorsal horns. In goldfish, 5‐HT label was confined to the ventral horn and TH label was mainly observed in a cell group located ventromedially. Two‐dimensional gel electrophoresis showed a gradual increase in protein number from sturgeons to gars to goldfish. In conclusion, the spinal cords of sturgeons and gars share many morphological and chemical features, distinguishing them from the goldfish spinal cord. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Maria Sen Mun Wai</name><affiliations><json:string>Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China</json:string></affiliations></json:item><json:item><name>Dietrich Ernst Lorke</name><affiliations><json:string>Department of Anatomy, FMHS, UAE University, Al Ain, United Arab Emirates</json:string><json:string>Institute of Anatomy II, Experimental Morphology, Hamburg University, Germany</json:string></affiliations></json:item><json:item><name>Aiqun Zhang</name><affiliations><json:string>Department of Surgery, Chinese PLA General Hospital, Beijing, China</json:string></affiliations></json:item><json:item><name>Hsiang‐Fu Kung</name><affiliations><json:string>Stanley Ho Centre for Emerging Infectious Diseases, School of Public Health, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China</json:string></affiliations></json:item><json:item><name>David T. 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The spinal cords of the chondrostean Acipenser schrenckii (Amur sturgeon), holostean Lepisosteus oculatus (spotted gar), and teleost Carassius auratus (goldfish) were, therefore, analyzed by immunohistochemistry, electron microscopy and two‐dimensional gel electrophoresis. Morphology showed numerous similarities between sturgeons and gars. In both, a dorsal column between the two dorsal horns was lacking, giving the grey matter an inverted Y‐shape. In goldfish, a small dorsal column was seen, the grey matter occupied a larger area, neuronal density was much higher, and a ventral commissure was apparent, which was absent in sturgeons and gars. In the white matter of sturgeons and gars, small caliber axons predominated, whereas larger axons were frequent in goldfish. Choline acetyltransferase immunoreactive neurons were prevalent in the ventral horns of all three fish, mainly in motoneurons, but stained fibers were only found in sturgeons and gars. γ‐aminobutyric acid positive cells were seen in both the ventral and the dorsal horns of all three fish. Distribution of serotonin (5‐HT) and tyrosine hydroxylase (TH) immunoreaction was similar in sturgeons and gars, being located in both the ventral and the dorsal horns. In goldfish, 5‐HT label was confined to the ventral horn and TH label was mainly observed in a cell group located ventromedially. Two‐dimensional gel electrophoresis showed a gradual increase in protein number from sturgeons to gars to goldfish. In conclusion, the spinal cords of sturgeons and gars share many morphological and chemical features, distinguishing them from the goldfish spinal cord. Microsc. Res. 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Res. Tech.</title><idno type="pISSN">1059-910X</idno><idno type="eISSN">1097-0029</idno><idno type="DOI">10.1002/(ISSN)1097-0029</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2007-12"></date><biblScope unit="volume">70</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="1079">1079</biblScope><biblScope unit="page" to="1090">1090</biblScope></imprint></monogr><idno type="istex">71220740ECA44AFBEB3567BD3ACC5EF1901B9673</idno><idno type="DOI">10.1002/jemt.20515</idno><idno type="ArticleID">JEMT20515</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2007</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Little is known about the spinal cords of phylogenetically ancient actinopterygeans. The spinal cords of the chondrostean Acipenser schrenckii (Amur sturgeon), holostean Lepisosteus oculatus (spotted gar), and teleost Carassius auratus (goldfish) were, therefore, analyzed by immunohistochemistry, electron microscopy and two‐dimensional gel electrophoresis. Morphology showed numerous similarities between sturgeons and gars. In both, a dorsal column between the two dorsal horns was lacking, giving the grey matter an inverted Y‐shape. In goldfish, a small dorsal column was seen, the grey matter occupied a larger area, neuronal density was much higher, and a ventral commissure was apparent, which was absent in sturgeons and gars. In the white matter of sturgeons and gars, small caliber axons predominated, whereas larger axons were frequent in goldfish. Choline acetyltransferase immunoreactive neurons were prevalent in the ventral horns of all three fish, mainly in motoneurons, but stained fibers were only found in sturgeons and gars. γ‐aminobutyric acid positive cells were seen in both the ventral and the dorsal horns of all three fish. Distribution of serotonin (5‐HT) and tyrosine hydroxylase (TH) immunoreaction was similar in sturgeons and gars, being located in both the ventral and the dorsal horns. In goldfish, 5‐HT label was confined to the ventral horn and TH label was mainly observed in a cell group located ventromedially. Two‐dimensional gel electrophoresis showed a gradual increase in protein number from sturgeons to gars to goldfish. In conclusion, the spinal cords of sturgeons and gars share many morphological and chemical features, distinguishing them from the goldfish spinal cord. Microsc. Res. 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Tech.</title></titleGroup></publicationMeta><publicationMeta level="part" position="120"><doi origin="wiley" registered="yes">10.1002/jemt.v70:12</doi><numberingGroup><numbering type="journalVolume" number="70">70</numbering><numbering type="journalIssue">12</numbering></numberingGroup><coverDate startDate="2007-12">December 2007</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="100" status="forIssue"><doi origin="wiley" registered="yes">10.1002/jemt.20515</doi><idGroup><id type="unit" value="JEMT20515"></id></idGroup><countGroup><count type="pageTotal" number="12"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title><title type="tocHeading1">Research Articles</title></titleGroup><copyright ownership="publisher">Copyright © 2007 Wiley‐Liss, Inc.</copyright><eventGroup><event type="manuscriptReceived" date="2007-05-17"></event><event type="manuscriptAccepted" date="2007-07-13"></event><event type="publishedOnlineEarlyUnpaginated" date="2007-09-14"></event><event type="firstOnline" date="2007-09-14"></event><event type="publishedOnlineFinalForm" date="2007-11-13"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-09"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-30"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-11-01"></event></eventGroup><numberingGroup><numbering type="pageFirst">1079</numbering><numbering type="pageLast">1090</numbering></numberingGroup><correspondenceTo>Department of Anatomy, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JEMT.JEMT20515.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="8"></count><count type="tableTotal" number="0"></count><count type="referenceTotal" number="47"></count><count type="wordTotal" number="1327"></count></countGroup><titleGroup><title type="main" xml:lang="en">Study of the spinal cords of the sturgeon <i>Acipenser schrenckii</i>, gar <i>Lepisosteus oculatus</i>, and goldfish <i>Carassius auratus</i> by morphological, immunohistochemical, and biochemical approaches</title><title type="short" xml:lang="en">Spinal Cords of Sturgeon, Gar, and Goldfish</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1"><personName><givenNames>Maria Sen Mun</givenNames><familyName>Wai</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af2 #af3"><personName><givenNames>Dietrich Ernst</givenNames><familyName>Lorke</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af4"><personName><givenNames>Aiqun</givenNames><familyName>Zhang</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af5"><personName><givenNames>Hsiang‐Fu</givenNames><familyName>Kung</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>David T.</givenNames><familyName>Yew</familyName></personName><contactDetails><email normalForm="david-yew@cuhk.edu.hk">david‐yew@cuhk.edu.hk</email></contactDetails></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="CN" type="organization"><unparsedAffiliation>Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="AE" type="organization"><unparsedAffiliation>Department of Anatomy, FMHS, UAE University, Al Ain, United Arab Emirates</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="DE" type="organization"><unparsedAffiliation>Institute of Anatomy II, Experimental Morphology, Hamburg University, Germany</unparsedAffiliation></affiliation><affiliation xml:id="af4" countryCode="CN" type="organization"><unparsedAffiliation>Department of Surgery, Chinese PLA General Hospital, Beijing, China</unparsedAffiliation></affiliation><affiliation xml:id="af5" countryCode="CN" type="organization"><unparsedAffiliation>Stanley Ho Centre for Emerging Infectious Diseases, School of Public Health, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">fish</keyword><keyword xml:id="kwd2">neurotransmitter</keyword><keyword xml:id="kwd3">protein electrophoresis</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Little is known about the spinal cords of phylogenetically ancient actinopterygeans. The spinal cords of the chondrostean <i>Acipenser schrenckii</i> (Amur sturgeon), holostean <i>Lepisosteus oculatus</i> (spotted gar), and teleost <i>Carassius auratus</i> (goldfish) were, therefore, analyzed by immunohistochemistry, electron microscopy and two‐dimensional gel electrophoresis. Morphology showed numerous similarities between sturgeons and gars. In both, a dorsal column between the two dorsal horns was lacking, giving the grey matter an inverted Y‐shape. In goldfish, a small dorsal column was seen, the grey matter occupied a larger area, neuronal density was much higher, and a ventral commissure was apparent, which was absent in sturgeons and gars. In the white matter of sturgeons and gars, small caliber axons predominated, whereas larger axons were frequent in goldfish. Choline acetyltransferase immunoreactive neurons were prevalent in the ventral horns of all three fish, mainly in motoneurons, but stained fibers were only found in sturgeons and gars. γ‐aminobutyric acid positive cells were seen in both the ventral and the dorsal horns of all three fish. Distribution of serotonin (5‐HT) and tyrosine hydroxylase (TH) immunoreaction was similar in sturgeons and gars, being located in both the ventral and the dorsal horns. In goldfish, 5‐HT label was confined to the ventral horn and TH label was mainly observed in a cell group located ventromedially. Two‐dimensional gel electrophoresis showed a gradual increase in protein number from sturgeons to gars to goldfish. In conclusion, the spinal cords of sturgeons and gars share many morphological and chemical features, distinguishing them from the goldfish spinal cord. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Study of the spinal cords of the sturgeon Acipenser schrenckii, gar Lepisosteus oculatus, and goldfish Carassius auratus by morphological, immunohistochemical, and biochemical approaches</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Spinal Cords of Sturgeon, Gar, and Goldfish</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Study of the spinal cords of the sturgeon Acipenser schrenckii, gar Lepisosteus oculatus, and goldfish Carassius auratus by morphological, immunohistochemical, and biochemical approaches</title></titleInfo><name type="personal"><namePart type="given">Maria Sen Mun</namePart><namePart type="family">Wai</namePart><affiliation>Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dietrich Ernst</namePart><namePart type="family">Lorke</namePart><affiliation>Department of Anatomy, FMHS, UAE University, Al Ain, United Arab Emirates</affiliation><affiliation>Institute of Anatomy II, Experimental Morphology, Hamburg University, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Aiqun</namePart><namePart type="family">Zhang</namePart><affiliation>Department of Surgery, Chinese PLA General Hospital, Beijing, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hsiang‐Fu</namePart><namePart type="family">Kung</namePart><affiliation>Stanley Ho Centre for Emerging Infectious Diseases, School of Public Health, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">David T.</namePart><namePart type="family">Yew</namePart><affiliation>Department of Anatomy, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China</affiliation><affiliation>Department of Anatomy, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2007-12</dateIssued><dateCaptured encoding="w3cdtf">2007-05-17</dateCaptured><dateValid encoding="w3cdtf">2007-07-13</dateValid><copyrightDate encoding="w3cdtf">2007</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">8</extent><extent unit="references">47</extent><extent unit="words">1327</extent></physicalDescription><abstract lang="en">Little is known about the spinal cords of phylogenetically ancient actinopterygeans. The spinal cords of the chondrostean Acipenser schrenckii (Amur sturgeon), holostean Lepisosteus oculatus (spotted gar), and teleost Carassius auratus (goldfish) were, therefore, analyzed by immunohistochemistry, electron microscopy and two‐dimensional gel electrophoresis. Morphology showed numerous similarities between sturgeons and gars. In both, a dorsal column between the two dorsal horns was lacking, giving the grey matter an inverted Y‐shape. In goldfish, a small dorsal column was seen, the grey matter occupied a larger area, neuronal density was much higher, and a ventral commissure was apparent, which was absent in sturgeons and gars. In the white matter of sturgeons and gars, small caliber axons predominated, whereas larger axons were frequent in goldfish. Choline acetyltransferase immunoreactive neurons were prevalent in the ventral horns of all three fish, mainly in motoneurons, but stained fibers were only found in sturgeons and gars. γ‐aminobutyric acid positive cells were seen in both the ventral and the dorsal horns of all three fish. Distribution of serotonin (5‐HT) and tyrosine hydroxylase (TH) immunoreaction was similar in sturgeons and gars, being located in both the ventral and the dorsal horns. In goldfish, 5‐HT label was confined to the ventral horn and TH label was mainly observed in a cell group located ventromedially. Two‐dimensional gel electrophoresis showed a gradual increase in protein number from sturgeons to gars to goldfish. In conclusion, the spinal cords of sturgeons and gars share many morphological and chemical features, distinguishing them from the goldfish spinal cord. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.</abstract><subject lang="en"><genre>keywords</genre><topic>fish</topic><topic>neurotransmitter</topic><topic>protein electrophoresis</topic></subject><relatedItem type="host"><titleInfo><title>Microscopy Research and Technique</title></titleInfo><titleInfo type="abbreviated"><title>Microsc. Res. Tech.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic></subject><identifier type="ISSN">1059-910X</identifier><identifier type="eISSN">1097-0029</identifier><identifier type="DOI">10.1002/(ISSN)1097-0029</identifier><identifier type="PublisherID">JEMT</identifier><part><date>2007</date><detail type="volume"><caption>vol.</caption><number>70</number></detail><detail type="issue"><caption>no.</caption><number>12</number></detail><extent unit="pages"><start>1079</start><end>1090</end><total>12</total></extent></part></relatedItem><identifier type="istex">71220740ECA44AFBEB3567BD3ACC5EF1901B9673</identifier><identifier type="DOI">10.1002/jemt.20515</identifier><identifier type="ArticleID">JEMT20515</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2007 Wiley‐Liss, Inc.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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