Renal l-gulono-1,4-lactone oxidase activity as affected by dietary ascorbic acid in lake sturgeon ( Acipenser fulvescens )
Identifieur interne : 000D85 ( Istex/Corpus ); précédent : 000D84; suivant : 000D86Renal l-gulono-1,4-lactone oxidase activity as affected by dietary ascorbic acid in lake sturgeon ( Acipenser fulvescens )
Auteurs : Régis Moreau ; Konrad Dabrowski ; Paul H. SatoSource :
- Aquaculture [ 0044-8486 ] ; 1999.
Abstract
Sturgeon can synthesize l-ascorbic acid (vitamin C) as they possess in their kidney l-gulono-1,4-lactone oxidase, the enzyme catalyzing the last step of AA biosynthesis. The effect of increasing dietary ascorbic acid on gulonolactone oxidase activity was studied in lake sturgeon. Two-year-old lake sturgeon (body weight 253±89 g) were fed in triplicate groups with casein-based semipurified diets supplemented with either 0, 50, 250 or 1250 mg ascorbic acid/kg in the form of ascorbyl-2-monophosphate Mg for 38 days at 19.8°C. At the end of the trial, there were no significant differences in growth rate and survival among groups. Tissue total ascorbic acid concentrations increased significantly with dietary ascorbic acid. Renal gulonolactone oxidase activity was inconsistently affected by dietary treatment. These results suggested that, in sturgeon kidney unlike in the livers of ascorbic acid-synthesizing mammals, dietary ascorbic acid did not exert a negative feedback control on gulonolactone oxidase activity and thus on ascorbic acid synthesized. Using in vitro kinetics data we estimated a theoretical biosynthetic rate of ascorbic acid of 17 μmol (or 3 mg) per kilogram body weight per day at 15°C in juvenile lake sturgeon.
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DOI: 10.1016/S0044-8486(99)00211-2
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Renal l-gulono-1,4-lactone oxidase activity as affected by dietary ascorbic acid in lake sturgeon ( Acipenser fulvescens )</title><author><name sortKey="Moreau, Regis" sort="Moreau, Regis" uniqKey="Moreau R" first="Régis" last="Moreau">Régis Moreau</name><affiliation><mods:affiliation>School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, OH 43210, USA</mods:affiliation></affiliation></author><author><name sortKey="Dabrowski, Konrad" sort="Dabrowski, Konrad" uniqKey="Dabrowski K" first="Konrad" last="Dabrowski">Konrad Dabrowski</name><affiliation><mods:affiliation>School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, OH 43210, USA</mods:affiliation></affiliation></author><author><name sortKey="Sato, Paul H" sort="Sato, Paul H" uniqKey="Sato P" first="Paul H" last="Sato">Paul H. Sato</name><affiliation><mods:affiliation>Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:9EAF8C1678F93280E58879FD22457BE47BE48924</idno><date when="1999" year="1999">1999</date><idno type="doi">10.1016/S0044-8486(99)00211-2</idno><idno type="url">https://api.istex.fr/document/9EAF8C1678F93280E58879FD22457BE47BE48924/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000D85</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000D85</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Renal l-gulono-1,4-lactone oxidase activity as affected by dietary ascorbic acid in lake sturgeon ( Acipenser fulvescens )</title><author><name sortKey="Moreau, Regis" sort="Moreau, Regis" uniqKey="Moreau R" first="Régis" last="Moreau">Régis Moreau</name><affiliation><mods:affiliation>School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, OH 43210, USA</mods:affiliation></affiliation></author><author><name sortKey="Dabrowski, Konrad" sort="Dabrowski, Konrad" uniqKey="Dabrowski K" first="Konrad" last="Dabrowski">Konrad Dabrowski</name><affiliation><mods:affiliation>School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, OH 43210, USA</mods:affiliation></affiliation></author><author><name sortKey="Sato, Paul H" sort="Sato, Paul H" uniqKey="Sato P" first="Paul H" last="Sato">Paul H. Sato</name><affiliation><mods:affiliation>Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Aquaculture</title><title level="j" type="abbrev">AQUA</title><idno type="ISSN">0044-8486</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">180</biblScope><biblScope unit="issue">3–4</biblScope><biblScope unit="page" from="359">359</biblScope><biblScope unit="page" to="372">372</biblScope></imprint><idno type="ISSN">0044-8486</idno></series><idno type="istex">9EAF8C1678F93280E58879FD22457BE47BE48924</idno><idno type="DOI">10.1016/S0044-8486(99)00211-2</idno><idno type="PII">S0044-8486(99)00211-2</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0044-8486</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sturgeon can synthesize l-ascorbic acid (vitamin C) as they possess in their kidney l-gulono-1,4-lactone oxidase, the enzyme catalyzing the last step of AA biosynthesis. The effect of increasing dietary ascorbic acid on gulonolactone oxidase activity was studied in lake sturgeon. Two-year-old lake sturgeon (body weight 253±89 g) were fed in triplicate groups with casein-based semipurified diets supplemented with either 0, 50, 250 or 1250 mg ascorbic acid/kg in the form of ascorbyl-2-monophosphate Mg for 38 days at 19.8°C. At the end of the trial, there were no significant differences in growth rate and survival among groups. Tissue total ascorbic acid concentrations increased significantly with dietary ascorbic acid. Renal gulonolactone oxidase activity was inconsistently affected by dietary treatment. These results suggested that, in sturgeon kidney unlike in the livers of ascorbic acid-synthesizing mammals, dietary ascorbic acid did not exert a negative feedback control on gulonolactone oxidase activity and thus on ascorbic acid synthesized. Using in vitro kinetics data we estimated a theoretical biosynthetic rate of ascorbic acid of 17 μmol (or 3 mg) per kilogram body weight per day at 15°C in juvenile lake sturgeon.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Régis Moreau</name><affiliations><json:string>School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, OH 43210, USA</json:string></affiliations></json:item><json:item><name>Konrad Dabrowski</name><affiliations><json:string>School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, OH 43210, USA</json:string></affiliations></json:item><json:item><name>Paul H Sato</name><affiliations><json:string>Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Ascorbic acid biosynthesis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Gulonolactone oxidase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Fish kidney</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Sturgeon</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Vitamin C requirement</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Sturgeon can synthesize l-ascorbic acid (vitamin C) as they possess in their kidney l-gulono-1,4-lactone oxidase, the enzyme catalyzing the last step of AA biosynthesis. The effect of increasing dietary ascorbic acid on gulonolactone oxidase activity was studied in lake sturgeon. Two-year-old lake sturgeon (body weight 253±89 g) were fed in triplicate groups with casein-based semipurified diets supplemented with either 0, 50, 250 or 1250 mg ascorbic acid/kg in the form of ascorbyl-2-monophosphate Mg for 38 days at 19.8°C. At the end of the trial, there were no significant differences in growth rate and survival among groups. Tissue total ascorbic acid concentrations increased significantly with dietary ascorbic acid. Renal gulonolactone oxidase activity was inconsistently affected by dietary treatment. These results suggested that, in sturgeon kidney unlike in the livers of ascorbic acid-synthesizing mammals, dietary ascorbic acid did not exert a negative feedback control on gulonolactone oxidase activity and thus on ascorbic acid synthesized. Using in vitro kinetics data we estimated a theoretical biosynthetic rate of ascorbic acid of 17 μmol (or 3 mg) per kilogram body weight per day at 15°C in juvenile lake sturgeon.</abstract><qualityIndicators><score>7.07</score><pdfVersion>1.2</pdfVersion><pdfPageSize>435 x 643 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1239</abstractCharCount><pdfWordCount>4862</pdfWordCount><pdfCharCount>29555</pdfCharCount><pdfPageCount>14</pdfPageCount><abstractWordCount>184</abstractWordCount></qualityIndicators><title>Renal l-gulono-1,4-lactone oxidase activity as affected by dietary ascorbic acid in lake sturgeon ( Acipenser fulvescens )</title><pii><json:string>S0044-8486(99)00211-2</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>180</volume><pii><json:string>S0044-8486(00)X0097-X</json:string></pii><pages><last>372</last><first>359</first></pages><issn><json:string>0044-8486</json:string></issn><issue>3–4</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Aquaculture</title><publicationDate>1999</publicationDate></host><categories><wos><json:string>science</json:string><json:string>marine & freshwater biology</json:string><json:string>fisheries</json:string></wos><scienceMetrix><json:string>applied sciences</json:string><json:string>agriculture, fisheries & forestry</json:string><json:string>fisheries</json:string></scienceMetrix></categories><publicationDate>1999</publicationDate><copyrightDate>1999</copyrightDate><doi><json:string>10.1016/S0044-8486(99)00211-2</json:string></doi><id>9EAF8C1678F93280E58879FD22457BE47BE48924</id><score>0.05055269</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/9EAF8C1678F93280E58879FD22457BE47BE48924/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/9EAF8C1678F93280E58879FD22457BE47BE48924/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/9EAF8C1678F93280E58879FD22457BE47BE48924/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Renal l-gulono-1,4-lactone oxidase activity as affected by dietary ascorbic acid in lake sturgeon ( Acipenser fulvescens )</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1999 Elsevier Science B.V.</p></availability><date>1999</date></publicationStmt><notesStmt><note type="content">Fig. 1: Effect of dietary AA on GLO activity. Correlations between posterior kidney GLO activity and tissue total AA concentrations in lake sturgeon fed graded levels of AA. Values represent means per tank. One degree of freedom was assigned to each tank. Labels indicate AA supplement expressed as mg/kg diet.</note><note type="content">Fig. 2: Effect of assay temperature on sturgeon GLO activity. In vitro synthesis of AA was measured at four temperatures in crude extracts prepared from lake sturgeon posterior kidney. Data are shown as mean±SD for three replicate measures. Q10 was calculated between 15°C and 25°C following linear regression.</note><note type="content">Fig. 3: Effect of l-gulonolatone (S) concentration on sturgeon GLO velocity (V) at 25°C. In vitro rate of AA synthesis as catalyzed by GLO purified from white sturgeon posterior kidney. (A) Lineweaver–Burk double reciprocal plot. (B) S/V against S plot. Data represent means for two replicate measures. Standard deviations lie within the symbols.</note><note type="content">Table 1: Composition of the basal diet</note><note type="content">Table 2: Growth rate and hepatosomatic index of lake sturgeon fed graded levels of AA. Data are presented as mean±SEM for three replicate tanks</note><note type="content">Table 3: Total (TAA), oxidized (DHAA), and reduced (AA) ascorbic acid concentrations in tissues of juvenile lake sturgeon fed graded levels of AA. Data are shown as means±SEM for three replicate tanks. Values not sharing a common letter within rows are significantly different (P<0.05)</note><note type="content">Table 4: GLO in posterior kidney, DHAA reductase in posterior kidney and liver, and total alkaline phosphatase in blood plasma of juvenile lake sturgeon fed graded levels of AA. Data are shown as means±SEM for three replicate tanks. GLO activities not sharing a common letter are significantly different (P<0.05)</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Renal l-gulono-1,4-lactone oxidase activity as affected by dietary ascorbic acid in lake sturgeon ( Acipenser fulvescens )</title><author xml:id="author-1"><persName><forename type="first">Régis</forename><surname>Moreau</surname></persName><affiliation>School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, OH 43210, USA</affiliation></author><author xml:id="author-2"><persName><forename type="first">Konrad</forename><surname>Dabrowski</surname></persName><affiliation>School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, OH 43210, USA</affiliation></author><author xml:id="author-3"><persName><forename type="first">Paul H</forename><surname>Sato</surname></persName><affiliation>Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA</affiliation></author></analytic><monogr><title level="j">Aquaculture</title><title level="j" type="abbrev">AQUA</title><idno type="pISSN">0044-8486</idno><idno type="PII">S0044-8486(00)X0097-X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999"></date><biblScope unit="volume">180</biblScope><biblScope unit="issue">3–4</biblScope><biblScope unit="page" from="359">359</biblScope><biblScope unit="page" to="372">372</biblScope></imprint></monogr><idno type="istex">9EAF8C1678F93280E58879FD22457BE47BE48924</idno><idno type="DOI">10.1016/S0044-8486(99)00211-2</idno><idno type="PII">S0044-8486(99)00211-2</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1999</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Sturgeon can synthesize l-ascorbic acid (vitamin C) as they possess in their kidney l-gulono-1,4-lactone oxidase, the enzyme catalyzing the last step of AA biosynthesis. The effect of increasing dietary ascorbic acid on gulonolactone oxidase activity was studied in lake sturgeon. Two-year-old lake sturgeon (body weight 253±89 g) were fed in triplicate groups with casein-based semipurified diets supplemented with either 0, 50, 250 or 1250 mg ascorbic acid/kg in the form of ascorbyl-2-monophosphate Mg for 38 days at 19.8°C. At the end of the trial, there were no significant differences in growth rate and survival among groups. Tissue total ascorbic acid concentrations increased significantly with dietary ascorbic acid. Renal gulonolactone oxidase activity was inconsistently affected by dietary treatment. These results suggested that, in sturgeon kidney unlike in the livers of ascorbic acid-synthesizing mammals, dietary ascorbic acid did not exert a negative feedback control on gulonolactone oxidase activity and thus on ascorbic acid synthesized. Using in vitro kinetics data we estimated a theoretical biosynthetic rate of ascorbic acid of 17 μmol (or 3 mg) per kilogram body weight per day at 15°C in juvenile lake sturgeon.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Ascorbic acid biosynthesis</term></item><item><term>Gulonolactone oxidase</term></item><item><term>Fish kidney</term></item><item><term>Sturgeon</term></item><item><term>Vitamin C requirement</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1999">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/9EAF8C1678F93280E58879FD22457BE47BE48924/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>AQUA</jid><aid>61021</aid><ce:pii>S0044-8486(99)00211-2</ce:pii><ce:doi>10.1016/S0044-8486(99)00211-2</ce:doi><ce:copyright year="1999" type="full-transfer">Elsevier Science B.V.</ce:copyright></item-info><head><ce:title>Renal <ce:small-caps>l</ce:small-caps>-gulono-1,4-lactone oxidase activity as affected by dietary ascorbic acid in lake sturgeon (<ce:italic>Acipenser fulvescens</ce:italic>)</ce:title><ce:author-group><ce:author><ce:given-name>Régis</ce:given-name><ce:surname>Moreau</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref></ce:author><ce:author><ce:given-name>Konrad</ce:given-name><ce:surname>Dabrowski</ce:surname><ce:cross-ref refid="AFF1">a</ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref></ce:author><ce:author><ce:given-name>Paul H</ce:given-name><ce:surname>Sato</ce:surname><ce:cross-ref refid="AFF2">b</ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, OH 43210, USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +1-614-292-4555; fax: +1-614-292-7432; E-mail: dabrowski.1@osu.edu</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="10" month="5" year="1999"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Sturgeon can synthesize <ce:small-caps>l</ce:small-caps>-ascorbic acid (vitamin C) as they possess in their kidney <ce:small-caps>l</ce:small-caps>-gulono-1,4-lactone oxidase, the enzyme catalyzing the last step of AA biosynthesis. The effect of increasing dietary ascorbic acid on gulonolactone oxidase activity was studied in lake sturgeon. Two-year-old lake sturgeon (body weight 253±89 g) were fed in triplicate groups with casein-based semipurified diets supplemented with either 0, 50, 250 or 1250 mg ascorbic acid/kg in the form of ascorbyl-2-monophosphate Mg for 38 days at 19.8°C. At the end of the trial, there were no significant differences in growth rate and survival among groups. Tissue total ascorbic acid concentrations increased significantly with dietary ascorbic acid. Renal gulonolactone oxidase activity was inconsistently affected by dietary treatment. These results suggested that, in sturgeon kidney unlike in the livers of ascorbic acid-synthesizing mammals, dietary ascorbic acid did not exert a negative feedback control on gulonolactone oxidase activity and thus on ascorbic acid synthesized. Using in vitro kinetics data we estimated a theoretical biosynthetic rate of ascorbic acid of 17 μmol (or 3 mg) per kilogram body weight per day at 15°C in juvenile lake sturgeon.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Ascorbic acid biosynthesis</ce:text></ce:keyword><ce:keyword><ce:text>Gulonolactone oxidase</ce:text></ce:keyword><ce:keyword><ce:text>Fish kidney</ce:text></ce:keyword><ce:keyword><ce:text>Sturgeon</ce:text></ce:keyword><ce:keyword><ce:text>Vitamin C requirement</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Renal l-gulono-1,4-lactone oxidase activity as affected by dietary ascorbic acid in lake sturgeon ( Acipenser fulvescens )</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Renal</title></titleInfo><name type="personal"><namePart type="given">Régis</namePart><namePart type="family">Moreau</namePart><affiliation>School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, OH 43210, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Konrad</namePart><namePart type="family">Dabrowski</namePart><affiliation>School of Natural Resources, The Ohio State University, 2021 Coffey Road, Columbus, OH 43210, USA</affiliation><description>Corresponding author. Tel.: +1-614-292-4555; fax: +1-614-292-7432; E-mail: dabrowski.1@osu.edu</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Paul H</namePart><namePart type="family">Sato</namePart><affiliation>Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1999</dateIssued><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Sturgeon can synthesize l-ascorbic acid (vitamin C) as they possess in their kidney l-gulono-1,4-lactone oxidase, the enzyme catalyzing the last step of AA biosynthesis. The effect of increasing dietary ascorbic acid on gulonolactone oxidase activity was studied in lake sturgeon. Two-year-old lake sturgeon (body weight 253±89 g) were fed in triplicate groups with casein-based semipurified diets supplemented with either 0, 50, 250 or 1250 mg ascorbic acid/kg in the form of ascorbyl-2-monophosphate Mg for 38 days at 19.8°C. At the end of the trial, there were no significant differences in growth rate and survival among groups. Tissue total ascorbic acid concentrations increased significantly with dietary ascorbic acid. Renal gulonolactone oxidase activity was inconsistently affected by dietary treatment. These results suggested that, in sturgeon kidney unlike in the livers of ascorbic acid-synthesizing mammals, dietary ascorbic acid did not exert a negative feedback control on gulonolactone oxidase activity and thus on ascorbic acid synthesized. Using in vitro kinetics data we estimated a theoretical biosynthetic rate of ascorbic acid of 17 μmol (or 3 mg) per kilogram body weight per day at 15°C in juvenile lake sturgeon.</abstract><note type="content">Fig. 1: Effect of dietary AA on GLO activity. Correlations between posterior kidney GLO activity and tissue total AA concentrations in lake sturgeon fed graded levels of AA. Values represent means per tank. One degree of freedom was assigned to each tank. Labels indicate AA supplement expressed as mg/kg diet.</note><note type="content">Fig. 2: Effect of assay temperature on sturgeon GLO activity. In vitro synthesis of AA was measured at four temperatures in crude extracts prepared from lake sturgeon posterior kidney. Data are shown as mean±SD for three replicate measures. Q10 was calculated between 15°C and 25°C following linear regression.</note><note type="content">Fig. 3: Effect of l-gulonolatone (S) concentration on sturgeon GLO velocity (V) at 25°C. In vitro rate of AA synthesis as catalyzed by GLO purified from white sturgeon posterior kidney. (A) Lineweaver–Burk double reciprocal plot. (B) S/V against S plot. Data represent means for two replicate measures. Standard deviations lie within the symbols.</note><note type="content">Table 1: Composition of the basal diet</note><note type="content">Table 2: Growth rate and hepatosomatic index of lake sturgeon fed graded levels of AA. Data are presented as mean±SEM for three replicate tanks</note><note type="content">Table 3: Total (TAA), oxidized (DHAA), and reduced (AA) ascorbic acid concentrations in tissues of juvenile lake sturgeon fed graded levels of AA. Data are shown as means±SEM for three replicate tanks. Values not sharing a common letter within rows are significantly different (P<0.05)</note><note type="content">Table 4: GLO in posterior kidney, DHAA reductase in posterior kidney and liver, and total alkaline phosphatase in blood plasma of juvenile lake sturgeon fed graded levels of AA. Data are shown as means±SEM for three replicate tanks. GLO activities not sharing a common letter are significantly different (P<0.05)</note><subject><genre>Keywords</genre><topic>Ascorbic acid biosynthesis</topic><topic>Gulonolactone oxidase</topic><topic>Fish kidney</topic><topic>Sturgeon</topic><topic>Vitamin C requirement</topic></subject><relatedItem type="host"><titleInfo><title>Aquaculture</title></titleInfo><titleInfo type="abbreviated"><title>AQUA</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">19991103</dateIssued></originInfo><identifier type="ISSN">0044-8486</identifier><identifier type="PII">S0044-8486(00)X0097-X</identifier><part><date>19991103</date><detail type="volume"><number>180</number><caption>vol.</caption></detail><detail type="issue"><number>3–4</number><caption>no.</caption></detail><extent unit="issue pages"><start>191</start><end>400</end></extent><extent unit="pages"><start>359</start><end>372</end></extent></part></relatedItem><identifier type="istex">9EAF8C1678F93280E58879FD22457BE47BE48924</identifier><identifier type="DOI">10.1016/S0044-8486(99)00211-2</identifier><identifier type="PII">S0044-8486(99)00211-2</identifier><accessCondition type="use and reproduction" contentType="copyright">©1999 Elsevier Science B.V.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science B.V., ©1999</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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