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Computer-assisted sperm analysis (CASA) as a tool for monitoring sperm quality in fish

Identifieur interne : 000984 ( Istex/Corpus ); précédent : 000983; suivant : 000985

Computer-assisted sperm analysis (CASA) as a tool for monitoring sperm quality in fish

Auteurs : D. E Kime ; K. J. W Van Look ; B. G Mcallister ; G. Huyskens ; E. Rurangwa ; F. Ollevier

Source :

RBID : ISTEX:1AEDBFB0670632473A0EDCAFAD00FFECF086BB47

English descriptors

Abstract

The use of motility as a measure of sperm quality in fish is reviewed. Computer assisted sperm analysis (CASA) provides a simple and rapid quantitative assessment of the quality of fish sperm and may predict its ability to fertilise eggs. It has been used to: monitor the effects of heavy metal pollutants, such as mercury and tributyltin, on sperm quality; to select broodstock; to improve the efficiency of cryopreservation and storage; and to optimise conditions for fertilisation. In combination with CASA, morphological measurements can be used to determine the causes of reduced sperm motility. Technical details for the use of CASA are described.

Url:
DOI: 10.1016/S1532-0456(01)00270-8

Links to Exploration step

ISTEX:1AEDBFB0670632473A0EDCAFAD00FFECF086BB47

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<ce:simple-para>The use of motility as a measure of sperm quality in fish is reviewed. Computer assisted sperm analysis (CASA) provides a simple and rapid quantitative assessment of the quality of fish sperm and may predict its ability to fertilise eggs. It has been used to: monitor the effects of heavy metal pollutants, such as mercury and tributyltin, on sperm quality; to select broodstock; to improve the efficiency of cryopreservation and storage; and to optimise conditions for fertilisation. In combination with CASA, morphological measurements can be used to determine the causes of reduced sperm motility. Technical details for the use of CASA are described.</ce:simple-para>
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<ce:keywords class="keyword">
<ce:section-title>Keywords</ce:section-title>
<ce:keyword>
<ce:text>Sperm</ce:text>
</ce:keyword>
<ce:keyword>
<ce:text>Fish</ce:text>
</ce:keyword>
<ce:keyword>
<ce:text>Motility</ce:text>
</ce:keyword>
<ce:keyword>
<ce:text>Fertilisation rate</ce:text>
</ce:keyword>
<ce:keyword>
<ce:text>Computer assisted sperm analysis (CASA)</ce:text>
</ce:keyword>
<ce:keyword>
<ce:text>Pollution</ce:text>
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<namePart type="given">D.E</namePart>
<namePart type="family">Kime</namePart>
<affiliation>Department of Animal and Plant Sciences, The University of Sheffield, Sheffield S10 2TN, UK</affiliation>
<affiliation>Corresponding author. Tel.: +44-114-222-4316; fax: +44-114-222-0002</affiliation>
<affiliation>E-mail: d.kime@sheffield.ac.uk</affiliation>
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<affiliation>Department of Animal and Plant Sciences, The University of Sheffield, Sheffield S10 2TN, UK</affiliation>
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<namePart type="family">Huyskens</namePart>
<affiliation>Laboratory of Aquatic Ecology, Katholieke Universiteit Leuven, Ch. De Beriotstraat 32, B-3000 Leuven, Belgium</affiliation>
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<affiliation>Laboratory of Aquatic Ecology, Katholieke Universiteit Leuven, Ch. De Beriotstraat 32, B-3000 Leuven, Belgium</affiliation>
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<affiliation>Laboratory of Aquatic Ecology, Katholieke Universiteit Leuven, Ch. De Beriotstraat 32, B-3000 Leuven, Belgium</affiliation>
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<abstract lang="en">The use of motility as a measure of sperm quality in fish is reviewed. Computer assisted sperm analysis (CASA) provides a simple and rapid quantitative assessment of the quality of fish sperm and may predict its ability to fertilise eggs. It has been used to: monitor the effects of heavy metal pollutants, such as mercury and tributyltin, on sperm quality; to select broodstock; to improve the efficiency of cryopreservation and storage; and to optimise conditions for fertilisation. In combination with CASA, morphological measurements can be used to determine the causes of reduced sperm motility. Technical details for the use of CASA are described.</abstract>
<note>This paper was originally presented at a symposium dedicated to the memory of Marcel Florkin, held within the ESCPB 21st International Congress, Liège, Belgium, July 24–28, 2000.</note>
<note type="content">Section title: Review</note>
<subject lang="en">
<genre>Keywords</genre>
<topic>Sperm</topic>
<topic>Fish</topic>
<topic>Motility</topic>
<topic>Fertilisation rate</topic>
<topic>Computer assisted sperm analysis (CASA)</topic>
<topic>Pollution</topic>
<topic>Crypreservation</topic>
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<dateIssued encoding="w3cdtf">200112</dateIssued>
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<identifier type="ISSN">1532-0456</identifier>
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<date>200112</date>
<detail type="volume">
<number>130</number>
<caption>vol.</caption>
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<number>4</number>
<caption>no.</caption>
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<identifier type="DOI">10.1016/S1532-0456(01)00270-8</identifier>
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