Studies on morphogenesis and cellular interactions of Rana grylio virus in an infected fish cell line
Identifieur interne : 000985 ( Istex/Corpus ); précédent : 000984; suivant : 000986Studies on morphogenesis and cellular interactions of Rana grylio virus in an infected fish cell line
Auteurs : Qi-Ya Zhang ; Zhen-Qiu Li ; Jian-Fang GuiSource :
- Aquaculture [ 0044-8486 ] ; 1999.
Abstract
A pathogenic virus (RGV), isolated from diseased pig frog Rana grylio with lethal syndrome, was investigated with regard to morphogenesis and cellular interactions in EPC cells, a cell line from fish. Different stages of virus amplification, maturation and assembly were observed at nucleus, cytoplasm and cellular membranes. The matured virus particles, were not only distributed diffusely in nucleus, cytoplasm and cellular surface, but also aggregated as pseudocrystalline arrays in the cytoplasm. Virions were released by budding from the plasma membranes, or following cell lysis. Various types of cell damage, such as small vacuoles, spherical inclusions, and swollen and empty mitochondria, were also found. Some typical characteristics of RGV, such as the symmetrical shape of the virions, replication process involving both nuclear and cytoplasmic phases, budding release from cellular membrane and intracellular membrane, viromatrix and paracrystalline aggregation in cytoplasm, and its acute pathogenic effects, were observed to be similar to that of other iridoviruses. Therefore, the RGV appears to be a member of the Iridoviridae based on these studies.
Url:
DOI: 10.1016/S0044-8486(99)00041-1
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Different stages of virus amplification, maturation and assembly were observed at nucleus, cytoplasm and cellular membranes. The matured virus particles, were not only distributed diffusely in nucleus, cytoplasm and cellular surface, but also aggregated as pseudocrystalline arrays in the cytoplasm. Virions were released by budding from the plasma membranes, or following cell lysis. Various types of cell damage, such as small vacuoles, spherical inclusions, and swollen and empty mitochondria, were also found. Some typical characteristics of RGV, such as the symmetrical shape of the virions, replication process involving both nuclear and cytoplasmic phases, budding release from cellular membrane and intracellular membrane, viromatrix and paracrystalline aggregation in cytoplasm, and its acute pathogenic effects, were observed to be similar to that of other iridoviruses. Therefore, the RGV appears to be a member of the Iridoviridae based on these studies.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Qi-Ya Zhang</name><affiliations><json:string>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</json:string></affiliations></json:item><json:item><name>Zhen-Qiu Li</name><affiliations><json:string>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</json:string></affiliations></json:item><json:item><name>Jian-Fang Gui</name><affiliations><json:string>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Rana grylio</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RGV</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Iridovirus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Morphogenesis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Fish cell line</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>A pathogenic virus (RGV), isolated from diseased pig frog Rana grylio with lethal syndrome, was investigated with regard to morphogenesis and cellular interactions in EPC cells, a cell line from fish. Different stages of virus amplification, maturation and assembly were observed at nucleus, cytoplasm and cellular membranes. The matured virus particles, were not only distributed diffusely in nucleus, cytoplasm and cellular surface, but also aggregated as pseudocrystalline arrays in the cytoplasm. Virions were released by budding from the plasma membranes, or following cell lysis. Various types of cell damage, such as small vacuoles, spherical inclusions, and swollen and empty mitochondria, were also found. Some typical characteristics of RGV, such as the symmetrical shape of the virions, replication process involving both nuclear and cytoplasmic phases, budding release from cellular membrane and intracellular membrane, viromatrix and paracrystalline aggregation in cytoplasm, and its acute pathogenic effects, were observed to be similar to that of other iridoviruses. Therefore, the RGV appears to be a member of the Iridoviridae based on these studies.</abstract><qualityIndicators><score>6.236</score><pdfVersion>1.2</pdfVersion><pdfPageSize>435 x 643 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1167</abstractCharCount><pdfWordCount>4220</pdfWordCount><pdfCharCount>25884</pdfCharCount><pdfPageCount>13</pdfPageCount><abstractWordCount>168</abstractWordCount></qualityIndicators><title>Studies on morphogenesis and cellular interactions of Rana grylio virus in an infected fish cell line</title><pii><json:string>S0044-8486(99)00041-1</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>175</volume><pii><json:string>S0044-8486(00)X0089-0</json:string></pii><pages><last>197</last><first>185</first></pages><issn><json:string>0044-8486</json:string></issn><issue>3–4</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Aquaculture</title><publicationDate>1999</publicationDate></host><publicationDate>1999</publicationDate><copyrightDate>1999</copyrightDate><doi><json:string>10.1016/S0044-8486(99)00041-1</json:string></doi><id>9F19C60C2DA9A19951DFC7DC88D2FD33E470EFB7</id><score>0.029659625</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/9F19C60C2DA9A19951DFC7DC88D2FD33E470EFB7/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/9F19C60C2DA9A19951DFC7DC88D2FD33E470EFB7/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/9F19C60C2DA9A19951DFC7DC88D2FD33E470EFB7/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Studies on morphogenesis and cellular interactions of Rana grylio virus in an infected fish cell line</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©1999 Elsevier Science B.V.</p></availability><date>1999</date></publicationStmt><notesStmt><note type="content">Fig. 1: Entry of RGV in the infected EPC cells. (A) Two virions (indicated by arrowheads) absorbed to cell surface observed from the infected cells at 1 h; (B) an infected cell with large numbers of intracytoplasmic vesicles in cytoplasm around cell membrane observed from the infected cells at 4 h. ×12,000.</note><note type="content">Fig. 2: Amplification and assembly of RGV in nucleus. (A) Nucleocapsids of different assembly stages in nucleus observed from the infected cell at 8 h; (B) large numbers of non-enveloped virus particles and two enveloped virions (indicated by arrowheads within a small vesicle) in a cell without nucleus. ×16,000.</note><note type="content">Fig. 3: Viromatrix in cytoplasm and different virus particles. (A) Empty capsids (EC), capsids with partial cores (PC) and matured nucleocapsids (MN) around the viromatrix that is composed of electron dense fiber-like materials observed from the infected cell at 12 h; (B) viromatrix that contains the matured nucleocapsids (MN) and empty capsids (EC), as well as the enveloped virions in a vesicle and a budding (indicated by arrowhead) virion into the vesicle in cytoplasm. ×24,000.</note><note type="content">Fig. 4: Formation process of RGV crystalline aggregation. The matured nucleocapsids were accumulated (A), then lined up (B), and then aggregated more and more (C), and finally formed crystals (D). These figures were obtained from the infected cells at 12 h. ×20,000.</note><note type="content">Fig. 5: Another aggregated crystal in the infected cell at 12 h, showing a curved parallel array of the RGV particles in cytoplasm. ×30,000.</note><note type="content">Fig. 6: Release of RGV from the infected cells. (A) Large numbers of budding virions (indicated by arrows) from one infected cell at the same time; (B) budding virions from tubular membrane protrusions (indicated by arrows); (C) desmosome-like junction between two neighboring cells in the RGV infected cells (indicated by arrow). These figures were obtained from the infected cells at 24 h. ×20,000.</note><note type="content">Fig. 7: Types of cell damages and different fates of mature viruses in the late of infection. (A) A lot of vacuoles and virus particles around the vacuole (indicated by arrowheads) in cytoplasm; (B) diffuse virus particles and spherical inclusion (indicated by arrowhead) in cytoplasm; (C) swollen and empty mitochondria and diffuse virus particles in cytoplasm; (D) membrane-enclosed virus particles (indicated by large arrowhead) after cell lysis. These figures were observed from the infected cells at 24 h. ×20,000.</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Studies on morphogenesis and cellular interactions of Rana grylio virus in an infected fish cell line</title><author xml:id="author-1"><persName><forename type="first">Qi-Ya</forename><surname>Zhang</surname></persName><affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</affiliation></author><author xml:id="author-2"><persName><forename type="first">Zhen-Qiu</forename><surname>Li</surname></persName><affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</affiliation></author><author xml:id="author-3"><persName><forename type="first">Jian-Fang</forename><surname>Gui</surname></persName><affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</affiliation></author></analytic><monogr><title level="j">Aquaculture</title><title level="j" type="abbrev">AQUA</title><idno type="pISSN">0044-8486</idno><idno type="PII">S0044-8486(00)X0089-0</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999"></date><biblScope unit="volume">175</biblScope><biblScope unit="issue">3–4</biblScope><biblScope unit="page" from="185">185</biblScope><biblScope unit="page" to="197">197</biblScope></imprint></monogr><idno type="istex">9F19C60C2DA9A19951DFC7DC88D2FD33E470EFB7</idno><idno type="DOI">10.1016/S0044-8486(99)00041-1</idno><idno type="PII">S0044-8486(99)00041-1</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1999</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>A pathogenic virus (RGV), isolated from diseased pig frog Rana grylio with lethal syndrome, was investigated with regard to morphogenesis and cellular interactions in EPC cells, a cell line from fish. Different stages of virus amplification, maturation and assembly were observed at nucleus, cytoplasm and cellular membranes. The matured virus particles, were not only distributed diffusely in nucleus, cytoplasm and cellular surface, but also aggregated as pseudocrystalline arrays in the cytoplasm. Virions were released by budding from the plasma membranes, or following cell lysis. Various types of cell damage, such as small vacuoles, spherical inclusions, and swollen and empty mitochondria, were also found. Some typical characteristics of RGV, such as the symmetrical shape of the virions, replication process involving both nuclear and cytoplasmic phases, budding release from cellular membrane and intracellular membrane, viromatrix and paracrystalline aggregation in cytoplasm, and its acute pathogenic effects, were observed to be similar to that of other iridoviruses. Therefore, the RGV appears to be a member of the Iridoviridae based on these studies.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Rana grylio</term></item><item><term>RGV</term></item><item><term>Iridovirus</term></item><item><term>Morphogenesis</term></item><item><term>Fish cell line</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1999">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/9F19C60C2DA9A19951DFC7DC88D2FD33E470EFB7/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity><istex:entity SYSTEM="gr4" NDATA="IMAGE" name="gr4"></istex:entity><istex:entity SYSTEM="gr5" NDATA="IMAGE" name="gr5"></istex:entity><istex:entity SYSTEM="gr6" NDATA="IMAGE" name="gr6"></istex:entity><istex:entity SYSTEM="gr7" NDATA="IMAGE" name="gr7"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>AQUA</jid><aid>60870</aid><ce:pii>S0044-8486(99)00041-1</ce:pii><ce:doi>10.1016/S0044-8486(99)00041-1</ce:doi><ce:copyright year="1999" type="full-transfer">Elsevier Science B.V.</ce:copyright></item-info><head><ce:title>Studies on morphogenesis and cellular interactions of <ce:italic>Rana grylio</ce:italic> virus in an infected fish cell line</ce:title><ce:author-group><ce:author><ce:given-name>Qi-Ya</ce:given-name><ce:surname>Zhang</ce:surname><ce:cross-ref refid="CORR1">*</ce:cross-ref></ce:author><ce:author><ce:given-name>Zhen-Qiu</ce:given-name><ce:surname>Li</ce:surname></ce:author><ce:author><ce:given-name>Jian-Fang</ce:given-name><ce:surname>Gui</ce:surname></ce:author><ce:affiliation><ce:textfn>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +86-027-87647707; E-mail: jfgui@lily.whihb.ac.cn</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="14" month="1" year="1999"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A pathogenic virus (RGV), isolated from diseased pig frog <ce:italic>Rana grylio</ce:italic> with lethal syndrome, was investigated with regard to morphogenesis and cellular interactions in EPC cells, a cell line from fish. Different stages of virus amplification, maturation and assembly were observed at nucleus, cytoplasm and cellular membranes. The matured virus particles, were not only distributed diffusely in nucleus, cytoplasm and cellular surface, but also aggregated as pseudocrystalline arrays in the cytoplasm. Virions were released by budding from the plasma membranes, or following cell lysis. Various types of cell damage, such as small vacuoles, spherical inclusions, and swollen and empty mitochondria, were also found. Some typical characteristics of RGV, such as the symmetrical shape of the virions, replication process involving both nuclear and cytoplasmic phases, budding release from cellular membrane and intracellular membrane, viromatrix and paracrystalline aggregation in cytoplasm, and its acute pathogenic effects, were observed to be similar to that of other iridoviruses. Therefore, the RGV appears to be a member of the Iridoviridae based on these studies.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text><ce:italic>Rana grylio</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>RGV</ce:text></ce:keyword><ce:keyword><ce:text>Iridovirus</ce:text></ce:keyword><ce:keyword><ce:text>Morphogenesis</ce:text></ce:keyword><ce:keyword><ce:text>Fish cell line</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Studies on morphogenesis and cellular interactions of Rana grylio virus in an infected fish cell line</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Studies on morphogenesis and cellular interactions of</title></titleInfo><name type="personal"><namePart type="given">Qi-Ya</namePart><namePart type="family">Zhang</namePart><affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</affiliation><description>Corresponding author. Tel.: +86-027-87647707; E-mail: jfgui@lily.whihb.ac.cn</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Zhen-Qiu</namePart><namePart type="family">Li</namePart><affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jian-Fang</namePart><namePart type="family">Gui</namePart><affiliation>State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1999</dateIssued><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">A pathogenic virus (RGV), isolated from diseased pig frog Rana grylio with lethal syndrome, was investigated with regard to morphogenesis and cellular interactions in EPC cells, a cell line from fish. Different stages of virus amplification, maturation and assembly were observed at nucleus, cytoplasm and cellular membranes. The matured virus particles, were not only distributed diffusely in nucleus, cytoplasm and cellular surface, but also aggregated as pseudocrystalline arrays in the cytoplasm. Virions were released by budding from the plasma membranes, or following cell lysis. Various types of cell damage, such as small vacuoles, spherical inclusions, and swollen and empty mitochondria, were also found. Some typical characteristics of RGV, such as the symmetrical shape of the virions, replication process involving both nuclear and cytoplasmic phases, budding release from cellular membrane and intracellular membrane, viromatrix and paracrystalline aggregation in cytoplasm, and its acute pathogenic effects, were observed to be similar to that of other iridoviruses. Therefore, the RGV appears to be a member of the Iridoviridae based on these studies.</abstract><note type="content">Fig. 1: Entry of RGV in the infected EPC cells. (A) Two virions (indicated by arrowheads) absorbed to cell surface observed from the infected cells at 1 h; (B) an infected cell with large numbers of intracytoplasmic vesicles in cytoplasm around cell membrane observed from the infected cells at 4 h. ×12,000.</note><note type="content">Fig. 2: Amplification and assembly of RGV in nucleus. (A) Nucleocapsids of different assembly stages in nucleus observed from the infected cell at 8 h; (B) large numbers of non-enveloped virus particles and two enveloped virions (indicated by arrowheads within a small vesicle) in a cell without nucleus. ×16,000.</note><note type="content">Fig. 3: Viromatrix in cytoplasm and different virus particles. (A) Empty capsids (EC), capsids with partial cores (PC) and matured nucleocapsids (MN) around the viromatrix that is composed of electron dense fiber-like materials observed from the infected cell at 12 h; (B) viromatrix that contains the matured nucleocapsids (MN) and empty capsids (EC), as well as the enveloped virions in a vesicle and a budding (indicated by arrowhead) virion into the vesicle in cytoplasm. ×24,000.</note><note type="content">Fig. 4: Formation process of RGV crystalline aggregation. The matured nucleocapsids were accumulated (A), then lined up (B), and then aggregated more and more (C), and finally formed crystals (D). These figures were obtained from the infected cells at 12 h. ×20,000.</note><note type="content">Fig. 5: Another aggregated crystal in the infected cell at 12 h, showing a curved parallel array of the RGV particles in cytoplasm. ×30,000.</note><note type="content">Fig. 6: Release of RGV from the infected cells. (A) Large numbers of budding virions (indicated by arrows) from one infected cell at the same time; (B) budding virions from tubular membrane protrusions (indicated by arrows); (C) desmosome-like junction between two neighboring cells in the RGV infected cells (indicated by arrow). These figures were obtained from the infected cells at 24 h. ×20,000.</note><note type="content">Fig. 7: Types of cell damages and different fates of mature viruses in the late of infection. (A) A lot of vacuoles and virus particles around the vacuole (indicated by arrowheads) in cytoplasm; (B) diffuse virus particles and spherical inclusion (indicated by arrowhead) in cytoplasm; (C) swollen and empty mitochondria and diffuse virus particles in cytoplasm; (D) membrane-enclosed virus particles (indicated by large arrowhead) after cell lysis. These figures were observed from the infected cells at 24 h. ×20,000.</note><subject><genre>Keywords</genre><topic>Rana grylio</topic><topic>RGV</topic><topic>Iridovirus</topic><topic>Morphogenesis</topic><topic>Fish cell line</topic></subject><relatedItem type="host"><titleInfo><title>Aquaculture</title></titleInfo><titleInfo type="abbreviated"><title>AQUA</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">19990515</dateIssued></originInfo><identifier type="ISSN">0044-8486</identifier><identifier type="PII">S0044-8486(00)X0089-0</identifier><part><date>19990515</date><detail type="volume"><number>175</number><caption>vol.</caption></detail><detail type="issue"><number>3–4</number><caption>no.</caption></detail><extent unit="issue pages"><start>185</start><end>368</end></extent><extent unit="pages"><start>185</start><end>197</end></extent></part></relatedItem><identifier type="istex">9F19C60C2DA9A19951DFC7DC88D2FD33E470EFB7</identifier><identifier type="DOI">10.1016/S0044-8486(99)00041-1</identifier><identifier type="PII">S0044-8486(99)00041-1</identifier><accessCondition type="use and reproduction" contentType="copyright">©1999 Elsevier Science B.V.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science B.V., ©1999</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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