Physico‐chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)
Identifieur interne : 000697 ( Istex/Corpus ); précédent : 000696; suivant : 000698Physico‐chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)
Auteurs : P. Li ; M. Rodina ; M. Hulak ; D. Gela ; M. Psenicka ; Z. H. Li ; O. LinhartSource :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 2011-04.
Abstract
Physico‐chemical properties (spermatozoa motility, spermatozoa concentration, seminal plasma protein concentration, osmolality, pH, ions) and sperm protein profiles were evaluated in three Acipenseriforme species [Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)]. The spermatozoa velocity was observed significantly highest in sterlet and lowest in Siberian sturgeon (P < 0.05). Spermatozoa velocity was positively correlated to pH and the concentration of Na+ in seminal plasma in all species examined. Other tested parameters, in particular the concentration of K+, expressed species specific patterns, which could be linked to taxonomical status of species. The protein profiles of seminal plasma indicated a protein band with a molecular weight of 32.6 kDa exclusively detected in paddlefish. In spermatozoa, 95 protein spots were detected in Siberian sturgeon and sterlet, respectively, whereas only 70 spots were observed in paddlefish. Each spermatozoa protein map expressed a strong species specific pattern, which offers a basic benchmark to trace molecular differences among Acipenseriformes. It can be concluded that results presented in this study could be applied for successful reproduction management and cryopreservation protocols of these endangered species.
Url:
DOI: 10.1111/j.1439-0426.2010.01634.x
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Li</name><affiliation><mods:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</mods:affiliation></affiliation><affiliation><mods:affiliation>Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, China</mods:affiliation></affiliation></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M." last="Rodina">M. 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Li</name><affiliation><mods:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</mods:affiliation></affiliation><affiliation><mods:affiliation>Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, China</mods:affiliation></affiliation></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M." last="Rodina">M. Rodina</name><affiliation><mods:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</mods:affiliation></affiliation></author><author><name sortKey="Hulak, M" sort="Hulak, M" uniqKey="Hulak M" first="M." last="Hulak">M. Hulak</name><affiliation><mods:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</mods:affiliation></affiliation></author><author><name sortKey="Gela, D" sort="Gela, D" uniqKey="Gela D" first="D." last="Gela">D. Gela</name><affiliation><mods:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</mods:affiliation></affiliation></author><author><name sortKey="Psenicka, M" sort="Psenicka, M" uniqKey="Psenicka M" first="M." last="Psenicka">M. Psenicka</name><affiliation><mods:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</mods:affiliation></affiliation></author><author><name sortKey="Li, Z H" sort="Li, Z H" uniqKey="Li Z" first="Z. H." last="Li">Z. H. Li</name><affiliation><mods:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</mods:affiliation></affiliation><affiliation><mods:affiliation>Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, China</mods:affiliation></affiliation></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O." last="Linhart">O. Linhart</name><affiliation><mods:affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2011-04">2011-04</date><biblScope unit="volume">27</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="673">673</biblScope><biblScope unit="page" to="677">677</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">D926A41A4B1E0FA3675F57E9BF81814D69960181</idno><idno type="DOI">10.1111/j.1439-0426.2010.01634.x</idno><idno type="ArticleID">JAI1634</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Physico‐chemical properties (spermatozoa motility, spermatozoa concentration, seminal plasma protein concentration, osmolality, pH, ions) and sperm protein profiles were evaluated in three Acipenseriforme species [Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)]. The spermatozoa velocity was observed significantly highest in sterlet and lowest in Siberian sturgeon (P < 0.05). Spermatozoa velocity was positively correlated to pH and the concentration of Na+ in seminal plasma in all species examined. Other tested parameters, in particular the concentration of K+, expressed species specific patterns, which could be linked to taxonomical status of species. The protein profiles of seminal plasma indicated a protein band with a molecular weight of 32.6 kDa exclusively detected in paddlefish. In spermatozoa, 95 protein spots were detected in Siberian sturgeon and sterlet, respectively, whereas only 70 spots were observed in paddlefish. Each spermatozoa protein map expressed a strong species specific pattern, which offers a basic benchmark to trace molecular differences among Acipenseriformes. 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Linhart</name><affiliations><json:string>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</json:string></affiliations></json:item></author><articleId><json:string>JAI1634</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Physico‐chemical properties (spermatozoa motility, spermatozoa concentration, seminal plasma protein concentration, osmolality, pH, ions) and sperm protein profiles were evaluated in three Acipenseriforme species [Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)]. The spermatozoa velocity was observed significantly highest in sterlet and lowest in Siberian sturgeon (P > 0.05). Spermatozoa velocity was positively correlated to pH and the concentration of Na+ in seminal plasma in all species examined. Other tested parameters, in particular the concentration of K+, expressed species specific patterns, which could be linked to taxonomical status of species. The protein profiles of seminal plasma indicated a protein band with a molecular weight of 32.6 kDa exclusively detected in paddlefish. In spermatozoa, 95 protein spots were detected in Siberian sturgeon and sterlet, respectively, whereas only 70 spots were observed in paddlefish. Each spermatozoa protein map expressed a strong species specific pattern, which offers a basic benchmark to trace molecular differences among Acipenseriformes. 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Ceske Budejovice, Vodnany, Czech Republic</affiliation><affiliation>Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, China</affiliation></author><author xml:id="author-2"><persName><forename type="first">M.</forename><surname>Rodina</surname></persName><affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</affiliation></author><author xml:id="author-3"><persName><forename type="first">M.</forename><surname>Hulak</surname></persName><affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</affiliation></author><author xml:id="author-4"><persName><forename type="first">D.</forename><surname>Gela</surname></persName><affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</affiliation></author><author xml:id="author-5"><persName><forename type="first">M.</forename><surname>Psenicka</surname></persName><affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</affiliation></author><author xml:id="author-6"><persName><forename type="first">Z. H.</forename><surname>Li</surname></persName><affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</affiliation><affiliation>Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, China</affiliation></author><author xml:id="author-7"><persName><forename type="first">O.</forename><surname>Linhart</surname></persName><affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</affiliation></author></analytic><monogr><title level="j">Journal of Applied Ichthyology</title><idno type="pISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><idno type="DOI">10.1111/(ISSN)1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2011-04"></date><biblScope unit="volume">27</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="673">673</biblScope><biblScope unit="page" to="677">677</biblScope></imprint></monogr><idno type="istex">D926A41A4B1E0FA3675F57E9BF81814D69960181</idno><idno type="DOI">10.1111/j.1439-0426.2010.01634.x</idno><idno type="ArticleID">JAI1634</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2011</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Physico‐chemical properties (spermatozoa motility, spermatozoa concentration, seminal plasma protein concentration, osmolality, pH, ions) and sperm protein profiles were evaluated in three Acipenseriforme species [Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)]. The spermatozoa velocity was observed significantly highest in sterlet and lowest in Siberian sturgeon (P < 0.05). Spermatozoa velocity was positively correlated to pH and the concentration of Na+ in seminal plasma in all species examined. Other tested parameters, in particular the concentration of K+, expressed species specific patterns, which could be linked to taxonomical status of species. The protein profiles of seminal plasma indicated a protein band with a molecular weight of 32.6 kDa exclusively detected in paddlefish. In spermatozoa, 95 protein spots were detected in Siberian sturgeon and sterlet, respectively, whereas only 70 spots were observed in paddlefish. Each spermatozoa protein map expressed a strong species specific pattern, which offers a basic benchmark to trace molecular differences among Acipenseriformes. 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Li, University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech Republic.
E‐mail: <email>pingli06@yahoo.com</email></correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JAI.JAI1634.pdf"></link></linkGroup></publicationMeta><contentMeta><unparsedEditorialHistory>Received: June 10, 2010 Accepted: November 25, 2010</unparsedEditorialHistory><countGroup><count type="figureTotal" number="4"></count><count type="tableTotal" number="1"></count></countGroup><titleGroup><title type="main">Physico‐chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (<i>Acipenser baerii</i>), sterlet (<i>Acipenser ruthenus</i>) and paddlefish (<i>Polyodon spathula</i>)</title><title type="shortAuthors">P. Li et al.</title><title type="short">Physico‐chemical properties and protein profiles of sperm</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1 #a2"><personName><givenNames>P.</givenNames><familyName>Li</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>M.</givenNames><familyName>Rodina</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>M.</givenNames><familyName>Hulak</familyName></personName></creator><creator creatorRole="author" xml:id="cr4" affiliationRef="#a1"><personName><givenNames>D.</givenNames><familyName>Gela</familyName></personName></creator><creator creatorRole="author" xml:id="cr5" affiliationRef="#a1"><personName><givenNames>M.</givenNames><familyName>Psenicka</familyName></personName></creator><creator creatorRole="author" xml:id="cr6" affiliationRef="#a1 #a2"><personName><givenNames>Z. H.</givenNames><familyName>Li</familyName></personName></creator><creator creatorRole="author" xml:id="cr7" affiliationRef="#a1"><personName><givenNames>O.</givenNames><familyName>Linhart</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CZ"><unparsedAffiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</unparsedAffiliation></affiliation><affiliation xml:id="a2" countryCode="CN"><unparsedAffiliation>Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, China</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Summary</title><p>Physico‐chemical properties (spermatozoa motility, spermatozoa concentration, seminal plasma protein concentration, osmolality, pH, ions) and sperm protein profiles were evaluated in three Acipenseriforme species [Siberian sturgeon (<i>Acipenser baerii</i>), sterlet (<i>Acipenser ruthenus</i>) and paddlefish (<i>Polyodon spathula</i>)]. The spermatozoa velocity was observed significantly highest in sterlet and lowest in Siberian sturgeon (P < 0.05). Spermatozoa velocity was positively correlated to pH and the concentration of Na<sup>+</sup> in seminal plasma in all species examined. Other tested parameters, in particular the concentration of K<sup>+</sup>, expressed species specific patterns, which could be linked to taxonomical status of species. The protein profiles of seminal plasma indicated a protein band with a molecular weight of 32.6 kDa exclusively detected in paddlefish. In spermatozoa, 95 protein spots were detected in Siberian sturgeon and sterlet, respectively, whereas only 70 spots were observed in paddlefish. Each spermatozoa protein map expressed a strong species specific pattern, which offers a basic benchmark to trace molecular differences among Acipenseriformes. It can be concluded that results presented in this study could be applied for successful reproduction management and cryopreservation protocols of these endangered species.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Physico‐chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Physico‐chemical properties and protein profiles of sperm</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Physico‐chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)</title></titleInfo><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Li</namePart><affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</affiliation><affiliation>Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Rodina</namePart><affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Hulak</namePart><affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D.</namePart><namePart type="family">Gela</namePart><affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Psenicka</namePart><affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Z. H.</namePart><namePart type="family">Li</namePart><affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</affiliation><affiliation>Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O.</namePart><namePart type="family">Linhart</namePart><affiliation>South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2011-04</dateIssued><edition>Received: June 10, 2010 Accepted: November 25, 2010</edition><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">4</extent><extent unit="tables">1</extent></physicalDescription><abstract lang="en">Physico‐chemical properties (spermatozoa motility, spermatozoa concentration, seminal plasma protein concentration, osmolality, pH, ions) and sperm protein profiles were evaluated in three Acipenseriforme species [Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula)]. The spermatozoa velocity was observed significantly highest in sterlet and lowest in Siberian sturgeon (P < 0.05). Spermatozoa velocity was positively correlated to pH and the concentration of Na+ in seminal plasma in all species examined. Other tested parameters, in particular the concentration of K+, expressed species specific patterns, which could be linked to taxonomical status of species. The protein profiles of seminal plasma indicated a protein band with a molecular weight of 32.6 kDa exclusively detected in paddlefish. In spermatozoa, 95 protein spots were detected in Siberian sturgeon and sterlet, respectively, whereas only 70 spots were observed in paddlefish. Each spermatozoa protein map expressed a strong species specific pattern, which offers a basic benchmark to trace molecular differences among Acipenseriformes. It can be concluded that results presented in this study could be applied for successful reproduction management and cryopreservation protocols of these endangered species.</abstract><relatedItem type="host"><titleInfo><title>Journal of Applied Ichthyology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0175-8659</identifier><identifier type="eISSN">1439-0426</identifier><identifier type="DOI">10.1111/(ISSN)1439-0426</identifier><identifier type="PublisherID">JAI</identifier><part><date>2011</date><detail type="title"><title>Proceedings of the 6th International Symposium on Sturgeon Wuhan, China October 25‐31, 2009</title></detail><detail type="volume"><caption>vol.</caption><number>27</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>673</start><end>677</end><total>5</total></extent></part></relatedItem><identifier type="istex">D926A41A4B1E0FA3675F57E9BF81814D69960181</identifier><identifier type="DOI">10.1111/j.1439-0426.2010.01634.x</identifier><identifier type="ArticleID">JAI1634</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2011 Blackwell Verlag, Berlin</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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