Application of PCR‐SSCP to Species Identification of Fishery Products
Identifieur interne : 000696 ( Istex/Corpus ); précédent : 000695; suivant : 000697Application of PCR‐SSCP to Species Identification of Fishery Products
Auteurs : Hartmut Rehbein ; Gabriele Kress ; Thomas SchmidtSource :
- Journal of the Science of Food and Agriculture [ 0022-5142 ] ; 1997-05.
English descriptors
- KwdEn :
- PCR, SSCP, canned fish, caviar, eel, fish, food, salmon, species identification, trout.
Abstract
A method of DNA analysis has been developed to verify authenticity of labelled raw material of canned fish or in products made from closely related fish species (tuna, eel, salmon, trout and sturgeon). Short segments (123–358 bp) of the mitochondrial cytochrome b gene were amplified by the polymerase chain reaction (PCR) and analysed by single strand conformation polymorphism (SSCP) to get species‐specific patterns of single‐stranded DNA (ssDNA). DNA strands were separated by polyacrylamide gel electrophoresis and visualised by silver staining. Differentiation between four eel species was possible. Each of three types of sturgeon caviar gave a characteristic pattern of ssDNA. Canned sardine, herring, tuna and other species expressed specific bands of ssDNA. © 1997 SCI.
Url:
DOI: 10.1002/(SICI)1097-0010(199705)74:1<35::AID-JSFA765>3.0.CO;2-2
Links to Exploration step
ISTEX:65F6116D23BA359901B093A0E758F6E5B3613F51Le document en format XML
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Short segments (123–358 bp) of the mitochondrial cytochrome b gene were amplified by the polymerase chain reaction (PCR) and analysed by single strand conformation polymorphism (SSCP) to get species‐specific patterns of single‐stranded DNA (ssDNA). DNA strands were separated by polyacrylamide gel electrophoresis and visualised by silver staining. Differentiation between four eel species was possible. Each of three types of sturgeon caviar gave a characteristic pattern of ssDNA. Canned sardine, herring, tuna and other species expressed specific bands of ssDNA. © 1997 SCI.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Hartmut Rehbein</name><affiliations><json:string>Institute of Biochemistry and Technology, Federal Research Centre for Fisheries, Palmaille 9, D‐22767 Hamburg, Germany</json:string><json:string>Institute of Biochemistry and Technology, Federal Research Centre for Fisheries, Palmaille 9, D‐22767 Hamburg, Germany</json:string></affiliations></json:item><json:item><name>Gabriele Kress</name><affiliations><json:string>Institute of Biochemistry and Technology, Federal Research Centre for Fisheries, Palmaille 9, D‐22767 Hamburg, Germany</json:string></affiliations></json:item><json:item><name>Thomas Schmidt</name><affiliations><json:string>Institute of Biochemistry and Technology, Federal Research Centre for Fisheries, Palmaille 9, D‐22767 Hamburg, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>PCR</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SSCP</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>eel</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>caviar</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>salmon</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>trout</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>canned fish</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>fish</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>food</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>species identification</value></json:item></subject><articleId><json:string>JSFA765</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>A method of DNA analysis has been developed to verify authenticity of labelled raw material of canned fish or in products made from closely related fish species (tuna, eel, salmon, trout and sturgeon). 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Canned sardine, herring, tuna and other species expressed specific bands of ssDNA. © 1997 SCI.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Application of PCR‐SSCP to Species Identification of Fishery Products</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>PCR‐SSCP for identification of fishery products</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Application of PCR‐SSCP to Species Identification of Fishery Products</title></titleInfo><name type="personal"><namePart type="given">Hartmut</namePart><namePart type="family">Rehbein</namePart><affiliation>Institute of Biochemistry and Technology, Federal Research Centre for Fisheries, Palmaille 9, D‐22767 Hamburg, Germany</affiliation><affiliation>Institute of Biochemistry and Technology, Federal Research Centre for Fisheries, Palmaille 9, D‐22767 Hamburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Gabriele</namePart><namePart type="family">Kress</namePart><affiliation>Institute of Biochemistry and Technology, Federal Research Centre for Fisheries, Palmaille 9, D‐22767 Hamburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Thomas</namePart><namePart type="family">Schmidt</namePart><affiliation>Institute of Biochemistry and Technology, Federal Research Centre for Fisheries, Palmaille 9, D‐22767 Hamburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>John Wiley & Sons, Ltd</publisher><place><placeTerm type="text">London</placeTerm></place><dateIssued encoding="w3cdtf">1997-05</dateIssued><dateCaptured encoding="w3cdtf">1996-03-08</dateCaptured><dateValid encoding="w3cdtf">1996-10-14</dateValid><copyrightDate encoding="w3cdtf">1997</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">5</extent><extent unit="tables">1</extent><extent unit="references">22</extent></physicalDescription><abstract lang="en">A method of DNA analysis has been developed to verify authenticity of labelled raw material of canned fish or in products made from closely related fish species (tuna, eel, salmon, trout and sturgeon). Short segments (123–358 bp) of the mitochondrial cytochrome b gene were amplified by the polymerase chain reaction (PCR) and analysed by single strand conformation polymorphism (SSCP) to get species‐specific patterns of single‐stranded DNA (ssDNA). DNA strands were separated by polyacrylamide gel electrophoresis and visualised by silver staining. Differentiation between four eel species was possible. Each of three types of sturgeon caviar gave a characteristic pattern of ssDNA. Canned sardine, herring, tuna and other species expressed specific bands of ssDNA. © 1997 SCI.</abstract><subject lang="en"><genre>keywords</genre><topic>PCR</topic><topic>SSCP</topic><topic>eel</topic><topic>caviar</topic><topic>salmon</topic><topic>trout</topic><topic>canned fish</topic><topic>fish</topic><topic>food</topic><topic>species identification</topic></subject><relatedItem type="host"><titleInfo><title>Journal of the Science of Food and Agriculture</title></titleInfo><titleInfo type="abbreviated"><title>J. Sci. 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