Differentiation of sturgeon species by PCR-RFLP
Identifieur interne : 000C56 ( Istex/Checkpoint ); précédent : 000C55; suivant : 000C57Differentiation of sturgeon species by PCR-RFLP
Auteurs : Christian Wolf [Suisse] ; Philipp Hübner [Suisse] ; Jürg Lüthy [Suisse]Source :
- Food Research International [ 0963-9969 ] ; 2000.
Abstract
A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNAGlu/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of Acipenser and Huso originating from Europe and Asia.
Url:
DOI: 10.1016/S0963-9969(99)00150-7
Affiliations:
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<front><div type="abstract" xml:lang="en">A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNAGlu/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of Acipenser and Huso originating from Europe and Asia.</div>
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