Differentiation of sturgeon species by PCR-RFLP
Identifieur interne : 000332 ( Istex/Curation ); précédent : 000331; suivant : 000333Differentiation of sturgeon species by PCR-RFLP
Auteurs : Christian Wolf [Suisse] ; Philipp Hübner [Suisse] ; Jürg Lüthy [Suisse]Source :
- Food Research International [ 0963-9969 ] ; 2000.
Abstract
A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNAGlu/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of Acipenser and Huso originating from Europe and Asia.
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DOI: 10.1016/S0963-9969(99)00150-7
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last="Lüthy">Jürg Lüthy</name><affiliation wicri:level="1"><mods:affiliation>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</mods:affiliation><country xml:lang="fr">Suisse</country><wicri:regionArea>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Food Research International</title><title level="j" type="abbrev">FRIN</title><idno type="ISSN">0963-9969</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">32</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="699">699</biblScope><biblScope unit="page" to="705">705</biblScope></imprint><idno type="ISSN">0963-9969</idno></series><idno type="istex">9035064769572DF94BBBAB7A2E67843AEC0D6F7B</idno><idno 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To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of Acipenser and Huso originating from Europe and Asia.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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