Differentiation of sturgeon species by PCR-RFLP
Identifieur interne : 000332 ( Istex/Corpus ); précédent : 000331; suivant : 000333Differentiation of sturgeon species by PCR-RFLP
Auteurs : Christian Wolf ; Philipp Hübner ; Jürg LüthySource :
- Food Research International [ 0963-9969 ] ; 2000.
Abstract
A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNAGlu/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of Acipenser and Huso originating from Europe and Asia.
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DOI: 10.1016/S0963-9969(99)00150-7
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Differentiation of sturgeon species by PCR-RFLP</title><author><name sortKey="Wolf, Christian" sort="Wolf, Christian" uniqKey="Wolf C" first="Christian" last="Wolf">Christian Wolf</name><affiliation><mods:affiliation>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: christian.wolf@ibc.unibe.ch</mods:affiliation></affiliation></author><author><name sortKey="Hubner, Philipp" sort="Hubner, Philipp" uniqKey="Hubner P" first="Philipp" last="Hübner">Philipp Hübner</name><affiliation><mods:affiliation>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</mods:affiliation></affiliation></author><author><name sortKey="Luthy, Jurg" sort="Luthy, Jurg" uniqKey="Luthy J" first="Jürg" last="Lüthy">Jürg Lüthy</name><affiliation><mods:affiliation>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:9035064769572DF94BBBAB7A2E67843AEC0D6F7B</idno><date when="2000" year="2000">2000</date><idno type="doi">10.1016/S0963-9969(99)00150-7</idno><idno type="url">https://api.istex.fr/document/9035064769572DF94BBBAB7A2E67843AEC0D6F7B/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000332</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000332</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Differentiation of sturgeon species by PCR-RFLP</title><author><name sortKey="Wolf, Christian" sort="Wolf, Christian" uniqKey="Wolf C" first="Christian" last="Wolf">Christian Wolf</name><affiliation><mods:affiliation>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: christian.wolf@ibc.unibe.ch</mods:affiliation></affiliation></author><author><name sortKey="Hubner, Philipp" sort="Hubner, Philipp" uniqKey="Hubner P" first="Philipp" last="Hübner">Philipp Hübner</name><affiliation><mods:affiliation>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</mods:affiliation></affiliation></author><author><name sortKey="Luthy, Jurg" sort="Luthy, Jurg" uniqKey="Luthy J" first="Jürg" last="Lüthy">Jürg Lüthy</name><affiliation><mods:affiliation>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Food Research International</title><title level="j" type="abbrev">FRIN</title><idno type="ISSN">0963-9969</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">32</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="699">699</biblScope><biblScope unit="page" to="705">705</biblScope></imprint><idno type="ISSN">0963-9969</idno></series><idno type="istex">9035064769572DF94BBBAB7A2E67843AEC0D6F7B</idno><idno type="DOI">10.1016/S0963-9969(99)00150-7</idno><idno type="PII">S0963-9969(99)00150-7</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0963-9969</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNAGlu/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of Acipenser and Huso originating from Europe and Asia.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Christian Wolf</name><affiliations><json:string>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</json:string><json:string>E-mail: christian.wolf@ibc.unibe.ch</json:string></affiliations></json:item><json:item><name>Philipp Hübner</name><affiliations><json:string>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</json:string></affiliations></json:item><json:item><name>Jürg Lüthy</name><affiliations><json:string>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Species identification</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Sturgeon</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Acipenser</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Caviar</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PCR</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RFLP</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cyt b</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PCR : polymerase chain reaction</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RFLP : restriction fragment length polymorphism</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cyt b : cytochrome b</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RE : restriction endonuclease</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SSCP : single strand conformation polymorphism</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>IEF : isoelectric focusing</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>SDS–PAGE : sodium dodecyl sulfate–polyacrylamide gel electrophoresis</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNAGlu/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of Acipenser and Huso originating from Europe and Asia.</abstract><qualityIndicators><score>4.186</score><pdfVersion>1.2</pdfVersion><pdfPageSize>595 x 794 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>14</keywordCount><abstractCharCount>552</abstractCharCount><pdfWordCount>3262</pdfWordCount><pdfCharCount>20901</pdfCharCount><pdfPageCount>7</pdfPageCount><abstractWordCount>77</abstractWordCount></qualityIndicators><title>Differentiation of sturgeon species by PCR-RFLP</title><pii><json:string>S0963-9969(99)00150-7</json:string></pii><genre><json:string>research-article</json:string></genre><host><volume>32</volume><pii><json:string>S0963-9969(00)X0039-7</json:string></pii><pages><last>705</last><first>699</first></pages><issn><json:string>0963-9969</json:string></issn><issue>10</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Food Research International</title><publicationDate>1999</publicationDate></host><categories><wos><json:string>science</json:string><json:string>food science & technology</json:string></wos><scienceMetrix><json:string>applied sciences</json:string><json:string>agriculture, fisheries & forestry</json:string><json:string>food science</json:string></scienceMetrix></categories><publicationDate>2000</publicationDate><copyrightDate>2000</copyrightDate><doi><json:string>10.1016/S0963-9969(99)00150-7</json:string></doi><id>9035064769572DF94BBBAB7A2E67843AEC0D6F7B</id><score>0.07092714</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/9035064769572DF94BBBAB7A2E67843AEC0D6F7B/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/9035064769572DF94BBBAB7A2E67843AEC0D6F7B/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/9035064769572DF94BBBAB7A2E67843AEC0D6F7B/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Differentiation of sturgeon species by PCR-RFLP</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©2000 Canadian Institute of Food Science and Technology</p></availability><date>2000</date></publicationStmt><notesStmt><note type="content">Fig. 1: Alignment of the 462 bp PCR-amplicons of 10 investigated sturgeon species. Priming sites are underlined.</note><note type="content">Fig. 2: Alignment of a 124 bp (corresponding to position 439-562 on the cytochrome b gene) part of the cyt b gene of four A. gueldenstaedti, three A. persicus and two EMBL database entries (AF006136: A, gueldenstaedti, AF0061155: A. persicus) following the 462 bp fragment.</note><note type="content">Fig. 3: RFLP analysis of the 462 bp PCR amplicon of 10 sturgeon species with the four restriction enzymes necessary for identification: 1, Huso huso; 2, A. gueldenstaedti; 3, A. stellatus; 4, A. schrencki; 5, A. naccari; 6, A. nudiventris; 7, A. persicus; 8, A. ruthenus; 9, A. baerii; 10, A. sturio.</note><note type="content">Fig. 4: RFLP pattern of caviar samples K1 — K23 after digestion with NlaIII (A), Rsa (B), SspI (C), and Tru9I (D). Results of restriction analysis are shown in Table 2.</note><note type="content">Table 1: Restriction fragment length polymorphism (RFLP) of the cyt b gene of 10 sturgeon speciesa</note><note type="content">Table 2: Results of the classification of restriction pattern of 15 commercial caviar samples with the restriction enzymes NlaIII, RsaI, SspI and Tru91a</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Differentiation of sturgeon species by PCR-RFLP</title><author xml:id="author-1"><persName><forename type="first">Christian</forename><surname>Wolf</surname></persName><email>christian.wolf@ibc.unibe.ch</email><note type="correspondence"><p>Corresponding author. Tel.: +41-31-631-4340; fax: +41-31-631-4887</p></note><affiliation>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</affiliation></author><author xml:id="author-2"><persName><forename type="first">Philipp</forename><surname>Hübner</surname></persName><note type="biography">Present address: Kantonales Labor Zürich, Postfach, 8030 Zürich, Switzerland.</note><affiliation>Present address: Kantonales Labor Zürich, Postfach, 8030 Zürich, Switzerland.</affiliation><affiliation>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</affiliation></author><author xml:id="author-3"><persName><forename type="first">Jürg</forename><surname>Lüthy</surname></persName><affiliation>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</affiliation></author></analytic><monogr><title level="j">Food Research International</title><title level="j" type="abbrev">FRIN</title><idno type="pISSN">0963-9969</idno><idno type="PII">S0963-9969(00)X0039-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000"></date><biblScope unit="volume">32</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="699">699</biblScope><biblScope unit="page" to="705">705</biblScope></imprint></monogr><idno type="istex">9035064769572DF94BBBAB7A2E67843AEC0D6F7B</idno><idno type="DOI">10.1016/S0963-9969(99)00150-7</idno><idno type="PII">S0963-9969(99)00150-7</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2000</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNAGlu/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of Acipenser and Huso originating from Europe and Asia.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Species identification</term></item><item><term>Sturgeon</term></item><item><term>Acipenser</term></item><item><term>Caviar</term></item><item><term>PCR</term></item><item><term>RFLP</term></item><item><term>cyt b</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>PCR</term><term>polymerase chain reaction</term></item><item><term>RFLP</term><term>restriction fragment length polymorphism</term></item><item><term>cyt b</term><term>cytochrome b</term></item><item><term>RE</term><term>restriction endonuclease</term></item><item><term>SSCP</term><term>single strand conformation polymorphism</term></item><item><term>IEF</term><term>isoelectric focusing</term></item><item><term>SDS–PAGE</term><term>sodium dodecyl sulfate–polyacrylamide gel electrophoresis</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2000">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/9035064769572DF94BBBAB7A2E67843AEC0D6F7B/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="gr3" NDATA="IMAGE" name="gr3"></istex:entity><istex:entity SYSTEM="gr4" NDATA="IMAGE" name="gr4"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>FRIN</jid><aid>1564</aid><ce:pii>S0963-9969(99)00150-7</ce:pii><ce:doi>10.1016/S0963-9969(99)00150-7</ce:doi><ce:copyright type="society" year="2000">Canadian Institute of Food Science and Technology</ce:copyright></item-info><head><ce:title>Differentiation of sturgeon species by PCR-RFLP</ce:title><ce:author-group><ce:author><ce:initials>C.</ce:initials><ce:given-name>Christian</ce:given-name><ce:surname>Wolf</ce:surname><ce:cross-ref refid="COR1">*</ce:cross-ref><ce:e-address>christian.wolf@ibc.unibe.ch</ce:e-address></ce:author><ce:author><ce:initials>P.</ce:initials><ce:given-name>Philipp</ce:given-name><ce:surname>Hübner</ce:surname><ce:cross-ref refid="FN1">1</ce:cross-ref></ce:author><ce:author><ce:initials>J.</ce:initials><ce:given-name>Jürg</ce:given-name><ce:surname>Lüthy</ce:surname></ce:author><ce:affiliation><ce:textfn>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +41-31-631-4340; fax: +41-31-631-4887</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>1</ce:label><ce:note-para>Present address: Kantonales Labor Zürich, Postfach, 8030 Zürich, Switzerland.</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="10" month="9" year="1999"></ce:date-received><ce:date-accepted day="11" month="11" year="1999"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNA<ce:sup>Glu</ce:sup>/cytochrome <ce:italic>b</ce:italic>) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of <ce:italic>Acipenser</ce:italic> and <ce:italic>Huso</ce:italic> originating from Europe and Asia.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Species identification</ce:text></ce:keyword><ce:keyword><ce:text>Sturgeon</ce:text></ce:keyword><ce:keyword><ce:text><ce:italic>Acipenser</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>Caviar</ce:text></ce:keyword><ce:keyword><ce:text>PCR</ce:text></ce:keyword><ce:keyword><ce:text>RFLP</ce:text></ce:keyword><ce:keyword><ce:text>cyt <ce:italic>b</ce:italic></ce:text></ce:keyword></ce:keywords><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>PCR</ce:text><ce:keyword><ce:text>polymerase chain reaction</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>RFLP</ce:text><ce:keyword><ce:text>restriction fragment length polymorphism</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>cyt <ce:italic>b</ce:italic></ce:text><ce:keyword><ce:text>cytochrome <ce:italic>b</ce:italic></ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>RE</ce:text><ce:keyword><ce:text>restriction endonuclease</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>SSCP</ce:text><ce:keyword><ce:text>single strand conformation polymorphism</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>IEF</ce:text><ce:keyword><ce:text>isoelectric focusing</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>SDS–PAGE</ce:text><ce:keyword><ce:text>sodium dodecyl sulfate–polyacrylamide gel electrophoresis</ce:text></ce:keyword></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Differentiation of sturgeon species by PCR-RFLP</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Differentiation of sturgeon species by PCR-RFLP</title></titleInfo><name type="personal"><namePart type="given">Christian</namePart><namePart type="family">Wolf</namePart><affiliation>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</affiliation><affiliation>E-mail: christian.wolf@ibc.unibe.ch</affiliation><description>Corresponding author. Tel.: +41-31-631-4340; fax: +41-31-631-4887</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Philipp</namePart><namePart type="family">Hübner</namePart><affiliation>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</affiliation><description>Present address: Kantonales Labor Zürich, Postfach, 8030 Zürich, Switzerland.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jürg</namePart><namePart type="family">Lüthy</namePart><affiliation>Laboratory of Food Chemistry, Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, 3012 Berne, Switzerland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2000</dateIssued><copyrightDate encoding="w3cdtf">2000</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">A method for identification of sturgeon species in caviar has been developed based on the amplification of a region of the mitochondrial genome (tRNAGlu/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several types of sturgeon caviar the obtained 462bp long PCR-products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphisms (RFLP). The method is suitable to differentiate between 10 species of Acipenser and Huso originating from Europe and Asia.</abstract><note type="content">Fig. 1: Alignment of the 462 bp PCR-amplicons of 10 investigated sturgeon species. Priming sites are underlined.</note><note type="content">Fig. 2: Alignment of a 124 bp (corresponding to position 439-562 on the cytochrome b gene) part of the cyt b gene of four A. gueldenstaedti, three A. persicus and two EMBL database entries (AF006136: A, gueldenstaedti, AF0061155: A. persicus) following the 462 bp fragment.</note><note type="content">Fig. 3: RFLP analysis of the 462 bp PCR amplicon of 10 sturgeon species with the four restriction enzymes necessary for identification: 1, Huso huso; 2, A. gueldenstaedti; 3, A. stellatus; 4, A. schrencki; 5, A. naccari; 6, A. nudiventris; 7, A. persicus; 8, A. ruthenus; 9, A. baerii; 10, A. sturio.</note><note type="content">Fig. 4: RFLP pattern of caviar samples K1 — K23 after digestion with NlaIII (A), Rsa (B), SspI (C), and Tru9I (D). Results of restriction analysis are shown in Table 2.</note><note type="content">Table 1: Restriction fragment length polymorphism (RFLP) of the cyt b gene of 10 sturgeon speciesa</note><note type="content">Table 2: Results of the classification of restriction pattern of 15 commercial caviar samples with the restriction enzymes NlaIII, RsaI, SspI and Tru91a</note><subject><genre>Keywords</genre><topic>Species identification</topic><topic>Sturgeon</topic><topic>Acipenser</topic><topic>Caviar</topic><topic>PCR</topic><topic>RFLP</topic><topic>cyt b</topic></subject><subject><genre>Abbreviations</genre><topic>PCR : polymerase chain reaction</topic><topic>RFLP : restriction fragment length polymorphism</topic><topic>cyt b : cytochrome b</topic><topic>RE : restriction endonuclease</topic><topic>SSCP : single strand conformation polymorphism</topic><topic>IEF : isoelectric focusing</topic><topic>SDS–PAGE : sodium dodecyl sulfate–polyacrylamide gel electrophoresis</topic></subject><relatedItem type="host"><titleInfo><title>Food Research International</title></titleInfo><titleInfo type="abbreviated"><title>FRIN</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199912</dateIssued></originInfo><identifier type="ISSN">0963-9969</identifier><identifier type="PII">S0963-9969(00)X0039-7</identifier><part><date>199912</date><detail type="volume"><number>32</number><caption>vol.</caption></detail><detail type="issue"><number>10</number><caption>no.</caption></detail><extent unit="issue pages"><start>659</start><end>722</end></extent><extent unit="pages"><start>699</start><end>705</end></extent></part></relatedItem><identifier type="istex">9035064769572DF94BBBAB7A2E67843AEC0D6F7B</identifier><identifier type="DOI">10.1016/S0963-9969(99)00150-7</identifier><identifier type="PII">S0963-9969(99)00150-7</identifier><accessCondition type="use and reproduction" contentType="copyright">©2000 Canadian Institute of Food Science and Technology</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Canadian Institute of Food Science and Technology, ©2000</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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|clé= ISTEX:9035064769572DF94BBBAB7A2E67843AEC0D6F7B
|texte= Differentiation of sturgeon species by PCR-RFLP
}}
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