Carrot (Daucus carota L.).
Identifieur interne : 000382 ( Main/Corpus ); précédent : 000381; suivant : 000383Carrot (Daucus carota L.).
Auteurs : Owen Wally ; Jayaraj Jayaraman ; Zamir K. PunjaSource :
- Methods in molecular biology (Clifton, N.J.) [ 1064-3745 ] ; 2006.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (cytology), Agrobacterium tumefaciens (genetics), Cell Culture Techniques (MeSH), Culture Media (MeSH), Daucus carota (genetics), Daucus carota (metabolism), Daucus carota (microbiology), Fungi (physiology), Genetic Vectors (MeSH), Immunity, Innate (genetics), Plant Diseases (microbiology), Plant Proteins (genetics), Plants, Genetically Modified (metabolism), Plants, Genetically Modified (microbiology), Polymerase Chain Reaction (MeSH), Proteomics (MeSH), Seedlings (genetics), Seedlings (metabolism), Seedlings (microbiology), Tissue Culture Techniques (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Plant Proteins.
- chemical : Culture Media.
- cytology : Agrobacterium tumefaciens.
- genetics : Agrobacterium tumefaciens, Daucus carota, Immunity, Innate, Seedlings.
- metabolism : Daucus carota, Plants, Genetically Modified, Seedlings.
- microbiology : Daucus carota, Plant Diseases, Plants, Genetically Modified, Seedlings.
- physiology : Fungi.
- Cell Culture Techniques, Genetic Vectors, Polymerase Chain Reaction, Proteomics, Tissue Culture Techniques, Transformation, Genetic.
Abstract
Plants are susceptible to infection by a broad range of fungal pathogens. Many horticulturally important crop species lack adequate genetic resistance to disease. Studies on potential mechanisms of disease resistance in plants have revealed the importance of a range of pathogenesis-related (PR) proteins with antifungal activity in reducing colonization of plant tissues by pathogens. We are evaluating a range of PR-proteins, through heterologous expression in transgenic carrot tissues, for their effects on fungal disease development. The protocols for carrot transformation with a thaumatin-like protein are described. In addition, the use of herbicide resistance as a selectable marker in carrot transformation is illustrated. In this protocol, petiole segments from carrot seedlings are exposed to Agrobacterium for 10-30 min and co-cultivated for 3 d, after which herbicide selection is imposed until embryogenic calli are produced after 8-12 wk. The transfer of the embryogenic calli to hormone-free medium yields transgenic plantlets. This genetic transformation protocol has supported the generation of transgenic carrot plants with defined T-DNA inserts at the rate of between 1 and 3 Southern positive independent events out of 100.
DOI: 10.1385/1-59745-131-2:3
PubMed: 17033046
Links to Exploration step
pubmed:17033046Le document en format XML
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Punja</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:17033046</idno><idno type="pmid">17033046</idno><idno type="doi">10.1385/1-59745-131-2:3</idno><idno type="wicri:Area/Main/Corpus">000382</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000382</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Carrot (Daucus carota L.).</title><author><name sortKey="Wally, Owen" sort="Wally, Owen" uniqKey="Wally O" first="Owen" last="Wally">Owen Wally</name><affiliation><nlm:affiliation>Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada.</nlm:affiliation></affiliation></author><author><name sortKey="Jayaraman, Jayaraj" sort="Jayaraman, Jayaraj" uniqKey="Jayaraman J" first="Jayaraj" last="Jayaraman">Jayaraj Jayaraman</name></author><author><name sortKey="Punja, Zamir K" sort="Punja, Zamir K" uniqKey="Punja Z" first="Zamir K" last="Punja">Zamir K. Punja</name></author></analytic><series><title level="j">Methods in molecular biology (Clifton, N.J.)</title><idno type="ISSN">1064-3745</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (cytology)</term><term>Agrobacterium tumefaciens (genetics)</term><term>Cell Culture Techniques (MeSH)</term><term>Culture Media (MeSH)</term><term>Daucus carota (genetics)</term><term>Daucus carota (metabolism)</term><term>Daucus carota (microbiology)</term><term>Fungi (physiology)</term><term>Genetic Vectors (MeSH)</term><term>Immunity, Innate (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Plants, Genetically Modified (microbiology)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Proteomics (MeSH)</term><term>Seedlings (genetics)</term><term>Seedlings (metabolism)</term><term>Seedlings (microbiology)</term><term>Tissue Culture Techniques (MeSH)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Agrobacterium tumefaciens</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Daucus carota</term><term>Immunity, Innate</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Daucus carota</term><term>Plants, Genetically Modified</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Daucus carota</term><term>Plant Diseases</term><term>Plants, Genetically Modified</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Culture Techniques</term><term>Genetic Vectors</term><term>Polymerase Chain Reaction</term><term>Proteomics</term><term>Tissue Culture Techniques</term><term>Transformation, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plants are susceptible to infection by a broad range of fungal pathogens. Many horticulturally important crop species lack adequate genetic resistance to disease. Studies on potential mechanisms of disease resistance in plants have revealed the importance of a range of pathogenesis-related (PR) proteins with antifungal activity in reducing colonization of plant tissues by pathogens. We are evaluating a range of PR-proteins, through heterologous expression in transgenic carrot tissues, for their effects on fungal disease development. The protocols for carrot transformation with a thaumatin-like protein are described. In addition, the use of herbicide resistance as a selectable marker in carrot transformation is illustrated. In this protocol, petiole segments from carrot seedlings are exposed to Agrobacterium for 10-30 min and co-cultivated for 3 d, after which herbicide selection is imposed until embryogenic calli are produced after 8-12 wk. The transfer of the embryogenic calli to hormone-free medium yields transgenic plantlets. This genetic transformation protocol has supported the generation of transgenic carrot plants with defined T-DNA inserts at the rate of between 1 and 3 Southern positive independent events out of 100.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17033046</PMID><DateCompleted><Year>2007</Year><Month>02</Month><Day>27</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1064-3745</ISSN><JournalIssue CitedMedium="Print"><Volume>344</Volume><PubDate><Year>2006</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Carrot (Daucus carota L.).</ArticleTitle><Pagination><MedlinePgn>3-12</MedlinePgn></Pagination><Abstract><AbstractText>Plants are susceptible to infection by a broad range of fungal pathogens. Many horticulturally important crop species lack adequate genetic resistance to disease. Studies on potential mechanisms of disease resistance in plants have revealed the importance of a range of pathogenesis-related (PR) proteins with antifungal activity in reducing colonization of plant tissues by pathogens. We are evaluating a range of PR-proteins, through heterologous expression in transgenic carrot tissues, for their effects on fungal disease development. The protocols for carrot transformation with a thaumatin-like protein are described. In addition, the use of herbicide resistance as a selectable marker in carrot transformation is illustrated. In this protocol, petiole segments from carrot seedlings are exposed to Agrobacterium for 10-30 min and co-cultivated for 3 d, after which herbicide selection is imposed until embryogenic calli are produced after 8-12 wk. The transfer of the embryogenic calli to hormone-free medium yields transgenic plantlets. This genetic transformation protocol has supported the generation of transgenic carrot plants with defined T-DNA inserts at the rate of between 1 and 3 Southern positive independent events out of 100.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wally</LastName><ForeName>Owen</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jayaraman</LastName><ForeName>Jayaraj</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Punja</LastName><ForeName>Zamir K</ForeName><Initials>ZK</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018929" MajorTopicYN="N">Cell Culture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018552" MajorTopicYN="N">Daucus carota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007113" MajorTopicYN="N">Immunity, Innate</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D036226" MajorTopicYN="N">Seedlings</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046509" MajorTopicYN="N">Tissue Culture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>10</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>2</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>10</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17033046</ArticleId><ArticleId IdType="pii">1-59745-131-2:3</ArticleId><ArticleId IdType="doi">10.1385/1-59745-131-2:3</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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