The apoplastic antioxidant system in Prunus: response to long-term plum pox virus infection.
Identifieur interne : 000381 ( Main/Corpus ); précédent : 000380; suivant : 000382The apoplastic antioxidant system in Prunus: response to long-term plum pox virus infection.
Auteurs : P. Diaz-Vivancos ; M. Rubio ; V. Mesonero ; P M Periago ; A Ros Barcel ; P. Martínez-G Mez ; J A HernándezSource :
- Journal of experimental botany [ 0022-0957 ] ; 2006.
English descriptors
- KwdEn :
- Antioxidants (metabolism), Ascorbate Peroxidases (MeSH), Catechol Oxidase (metabolism), Hydrogen Peroxide (metabolism), Oxidative Stress (MeSH), Peroxidase (metabolism), Peroxidases (metabolism), Plant Leaves (metabolism), Plant Leaves (virology), Plant Proteins (metabolism), Plum Pox Virus (physiology), Prunus (enzymology), Prunus (metabolism), Prunus (virology), Superoxide Dismutase (metabolism).
- MESH :
- chemical , metabolism : Antioxidants, Catechol Oxidase, Hydrogen Peroxide, Peroxidase, Peroxidases, Plant Proteins, Superoxide Dismutase.
- chemical : Ascorbate Peroxidases.
- enzymology : Prunus.
- metabolism : Plant Leaves, Prunus.
- physiology : Plum Pox Virus.
- virology : Plant Leaves, Prunus.
- Oxidative Stress.
Abstract
This work describes, for the first time, the changes taking place in the antioxidative system of the leaf apoplast in response to plum pox virus (PPV) in different Prunus species showing different susceptibilities to PPV. The presence of p-hydroxymercuribenzoic acid (pHMB)-sensitive ascorbate peroxidase (APX) (class I APX) and pHMB-insensitive APX (class III APX), superoxide dismutase (SOD), peroxidase (POX), NADH-POX, and polyphenoloxidase (PPO) was described in the apoplast from both peach and apricot leaves. PPV infection produced different changes in the antioxidant system of the leaf apoplast from the Prunus species, depending on their susceptibility to the virus. In leaves of the very susceptible peach cultivar GF305, PPV brought about an increase in class I APX, POX, NADH-POX, and PPO activities. In the susceptible apricot cultivar Real Fino, PPV infection produced a decrease in apoplastic POX and SOD activities, whereas a strong increase in PPO was observed. However, in the resistant apricot cultivar Stark Early Orange, a rise in class I APX as well as a strong increase in POX and SOD activities was noticed in the apoplastic compartment. Long-term PPV infection produced an oxidative stress in the apoplastic space from apricot and peach plants, as observed by the increase in H2O2 contents in this compartment. However, this increase was much higher in the PPV-susceptible plants than in the resistant apricot cultivar. Only in the PPV-susceptible apricot and peach plants was the increase in apoplastic H2O2 levels accompanied by an increase in electrolyte leakage. No changes in the electrolyte leakage were observed in the PPV-inoculated resistant apricot leaves, although a 42% increase in the apoplastic H2O2 levels was produced. Two-dimensional electrophoresis analyses revealed that the majority of the polypeptides in the apoplastic fluid had isoelectric points in the range of pI 4-6. The identification of proteins using MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) and peptide mass fingerprinting analyses showed the induction of a thaumatin-like protein as well as the decrease of mandelonitrile lyase in peach apoplast due to PPV infection. However, most of the selected polypeptides showed no homology with known proteins. This fact emphasizes that, at least in Prunus, most of the functions of the apoplastic space remain unknown. It is concluded that long-term PPV infection produced an oxidative stress in the leaf apoplast, contributing to the deleterious effects produced by PPV infection in leaves of inoculated, susceptible Prunus plants.
DOI: 10.1093/jxb/erl138
PubMed: 17043083
Links to Exploration step
pubmed:17043083Le document en format XML
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<author><name sortKey="Diaz Vivancos, P" sort="Diaz Vivancos, P" uniqKey="Diaz Vivancos P" first="P" last="Diaz-Vivancos">P. Diaz-Vivancos</name>
<affiliation><nlm:affiliation>Department of Plant Breeding. CEBAS-CSIC, PO Box 164, E-30100 Espinardo, Murcia, Spain.</nlm:affiliation>
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<author><name sortKey="Rubio, M" sort="Rubio, M" uniqKey="Rubio M" first="M" last="Rubio">M. Rubio</name>
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<author><name sortKey="Mesonero, V" sort="Mesonero, V" uniqKey="Mesonero V" first="V" last="Mesonero">V. Mesonero</name>
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<author><name sortKey="Periago, P M" sort="Periago, P M" uniqKey="Periago P" first="P M" last="Periago">P M Periago</name>
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<author><name sortKey="Barcel, A Ros" sort="Barcel, A Ros" uniqKey="Barcel A" first="A Ros" last="Barcel">A Ros Barcel</name>
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<author><name sortKey="Martinez G Mez, P" sort="Martinez G Mez, P" uniqKey="Martinez G Mez P" first="P" last="Martínez-G Mez">P. Martínez-G Mez</name>
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<author><name sortKey="Hernandez, J A" sort="Hernandez, J A" uniqKey="Hernandez J" first="J A" last="Hernández">J A Hernández</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">The apoplastic antioxidant system in Prunus: response to long-term plum pox virus infection.</title>
<author><name sortKey="Diaz Vivancos, P" sort="Diaz Vivancos, P" uniqKey="Diaz Vivancos P" first="P" last="Diaz-Vivancos">P. Diaz-Vivancos</name>
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<author><name sortKey="Periago, P M" sort="Periago, P M" uniqKey="Periago P" first="P M" last="Periago">P M Periago</name>
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<author><name sortKey="Martinez G Mez, P" sort="Martinez G Mez, P" uniqKey="Martinez G Mez P" first="P" last="Martínez-G Mez">P. Martínez-G Mez</name>
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<term>Ascorbate Peroxidases (MeSH)</term>
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<term>Hydrogen Peroxide (metabolism)</term>
<term>Oxidative Stress (MeSH)</term>
<term>Peroxidase (metabolism)</term>
<term>Peroxidases (metabolism)</term>
<term>Plant Leaves (metabolism)</term>
<term>Plant Leaves (virology)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plum Pox Virus (physiology)</term>
<term>Prunus (enzymology)</term>
<term>Prunus (metabolism)</term>
<term>Prunus (virology)</term>
<term>Superoxide Dismutase (metabolism)</term>
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<term>Peroxidases</term>
<term>Plant Proteins</term>
<term>Superoxide Dismutase</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Plum Pox Virus</term>
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<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Plant Leaves</term>
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<front><div type="abstract" xml:lang="en">This work describes, for the first time, the changes taking place in the antioxidative system of the leaf apoplast in response to plum pox virus (PPV) in different Prunus species showing different susceptibilities to PPV. The presence of p-hydroxymercuribenzoic acid (pHMB)-sensitive ascorbate peroxidase (APX) (class I APX) and pHMB-insensitive APX (class III APX), superoxide dismutase (SOD), peroxidase (POX), NADH-POX, and polyphenoloxidase (PPO) was described in the apoplast from both peach and apricot leaves. PPV infection produced different changes in the antioxidant system of the leaf apoplast from the Prunus species, depending on their susceptibility to the virus. In leaves of the very susceptible peach cultivar GF305, PPV brought about an increase in class I APX, POX, NADH-POX, and PPO activities. In the susceptible apricot cultivar Real Fino, PPV infection produced a decrease in apoplastic POX and SOD activities, whereas a strong increase in PPO was observed. However, in the resistant apricot cultivar Stark Early Orange, a rise in class I APX as well as a strong increase in POX and SOD activities was noticed in the apoplastic compartment. Long-term PPV infection produced an oxidative stress in the apoplastic space from apricot and peach plants, as observed by the increase in H2O2 contents in this compartment. However, this increase was much higher in the PPV-susceptible plants than in the resistant apricot cultivar. Only in the PPV-susceptible apricot and peach plants was the increase in apoplastic H2O2 levels accompanied by an increase in electrolyte leakage. No changes in the electrolyte leakage were observed in the PPV-inoculated resistant apricot leaves, although a 42% increase in the apoplastic H2O2 levels was produced. Two-dimensional electrophoresis analyses revealed that the majority of the polypeptides in the apoplastic fluid had isoelectric points in the range of pI 4-6. The identification of proteins using MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) and peptide mass fingerprinting analyses showed the induction of a thaumatin-like protein as well as the decrease of mandelonitrile lyase in peach apoplast due to PPV infection. However, most of the selected polypeptides showed no homology with known proteins. This fact emphasizes that, at least in Prunus, most of the functions of the apoplastic space remain unknown. It is concluded that long-term PPV infection produced an oxidative stress in the leaf apoplast, contributing to the deleterious effects produced by PPV infection in leaves of inoculated, susceptible Prunus plants.</div>
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<Abstract><AbstractText>This work describes, for the first time, the changes taking place in the antioxidative system of the leaf apoplast in response to plum pox virus (PPV) in different Prunus species showing different susceptibilities to PPV. The presence of p-hydroxymercuribenzoic acid (pHMB)-sensitive ascorbate peroxidase (APX) (class I APX) and pHMB-insensitive APX (class III APX), superoxide dismutase (SOD), peroxidase (POX), NADH-POX, and polyphenoloxidase (PPO) was described in the apoplast from both peach and apricot leaves. PPV infection produced different changes in the antioxidant system of the leaf apoplast from the Prunus species, depending on their susceptibility to the virus. In leaves of the very susceptible peach cultivar GF305, PPV brought about an increase in class I APX, POX, NADH-POX, and PPO activities. In the susceptible apricot cultivar Real Fino, PPV infection produced a decrease in apoplastic POX and SOD activities, whereas a strong increase in PPO was observed. However, in the resistant apricot cultivar Stark Early Orange, a rise in class I APX as well as a strong increase in POX and SOD activities was noticed in the apoplastic compartment. Long-term PPV infection produced an oxidative stress in the apoplastic space from apricot and peach plants, as observed by the increase in H2O2 contents in this compartment. However, this increase was much higher in the PPV-susceptible plants than in the resistant apricot cultivar. Only in the PPV-susceptible apricot and peach plants was the increase in apoplastic H2O2 levels accompanied by an increase in electrolyte leakage. No changes in the electrolyte leakage were observed in the PPV-inoculated resistant apricot leaves, although a 42% increase in the apoplastic H2O2 levels was produced. Two-dimensional electrophoresis analyses revealed that the majority of the polypeptides in the apoplastic fluid had isoelectric points in the range of pI 4-6. The identification of proteins using MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) and peptide mass fingerprinting analyses showed the induction of a thaumatin-like protein as well as the decrease of mandelonitrile lyase in peach apoplast due to PPV infection. However, most of the selected polypeptides showed no homology with known proteins. This fact emphasizes that, at least in Prunus, most of the functions of the apoplastic space remain unknown. It is concluded that long-term PPV infection produced an oxidative stress in the leaf apoplast, contributing to the deleterious effects produced by PPV infection in leaves of inoculated, susceptible Prunus plants.</AbstractText>
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