Transcriptome Analysis of Apple Leaves Infected by the Rust Fungus Gymnosporangium yamadae at Two Sporulation Stages.
Identifieur interne : 000025 ( Main/Corpus ); précédent : 000024; suivant : 000026Transcriptome Analysis of Apple Leaves Infected by the Rust Fungus Gymnosporangium yamadae at Two Sporulation Stages.
Auteurs : Si-Qi Tao ; Lucas Auer ; Emmanuelle Morin ; Ying-Mei Liang ; Sébastien DuplessisSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2020.
English descriptors
- KwdEn :
- MESH :
- metabolism : Malus, Plant Leaves.
- microbiology : Malus, Plant Diseases, Plant Leaves.
- pathogenicity : Basidiomycota.
- Gene Expression Profiling, Transcriptome.
Abstract
Apple rust disease caused by Gymnosporangium yamadae is one of the major threats to apple orchards. In this study, dual RNA-seq analysis was conducted to simultaneously monitor gene expression profiles of G. yamadae and infected apple leaves during the formation of rust spermogonia and aecia. The molecular mechanisms underlying this compatible interaction at 10 and 30 days postinoculation (dpi) indicate a significant reaction from the host plant and comprise detoxication pathways at the earliest stage and the induction of secondary metabolism pathways at 30 dpi. Such host reactions have been previously reported in other rust pathosystems and may represent a general reaction to rust infection. G. yamadae transcript profiling indicates a conserved genetic program in spermogonia and aecia that is shared with other rust fungi, whereas secretome prediction reveals the presence of specific secreted candidate effector proteins expressed during apple infection. Unexpectedly, the survey of fungal unigenes in the transcriptome assemblies of inoculated and mock-inoculated apple leaves reveals that G. yamadae infection may modify the fungal community composition in the apple phyllosphere at 30 dpi. Collectively, our results provide novel insights into the compatible apple-G. yamadae interaction and advance the knowledge of this heteroecious demicyclic rust fungus.
DOI: 10.1094/MPMI-07-19-0208-R
PubMed: 31765287
Links to Exploration step
pubmed:31765287Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transcriptome Analysis of Apple Leaves Infected by the Rust Fungus <i>Gymnosporangium yamadae</i> at Two Sporulation Stages.</title><author><name sortKey="Tao, Si Qi" sort="Tao, Si Qi" uniqKey="Tao S" first="Si-Qi" last="Tao">Si-Qi Tao</name><affiliation><nlm:affiliation>The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Université de Lorraine, INRAE, Unité Mixte de Recherche 1136 Interactions Arbres-Microorganismes, 54280, Champenoux, France.</nlm:affiliation></affiliation></author><author><name sortKey="Auer, Lucas" sort="Auer, Lucas" uniqKey="Auer L" first="Lucas" last="Auer">Lucas Auer</name><affiliation><nlm:affiliation>Université de Lorraine, INRAE, Unité Mixte de Recherche 1136 Interactions Arbres-Microorganismes, 54280, Champenoux, France.</nlm:affiliation></affiliation></author><author><name sortKey="Morin, Emmanuelle" sort="Morin, Emmanuelle" uniqKey="Morin E" first="Emmanuelle" last="Morin">Emmanuelle Morin</name><affiliation><nlm:affiliation>Université de Lorraine, INRAE, Unité Mixte de Recherche 1136 Interactions Arbres-Microorganismes, 54280, Champenoux, France.</nlm:affiliation></affiliation></author><author><name sortKey="Liang, Ying Mei" sort="Liang, Ying Mei" uniqKey="Liang Y" first="Ying-Mei" last="Liang">Ying-Mei Liang</name><affiliation><nlm:affiliation>Museum of Beijing Forestry University, Beijing Forestry University.</nlm:affiliation></affiliation></author><author><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><affiliation><nlm:affiliation>Université de Lorraine, INRAE, Unité Mixte de Recherche 1136 Interactions Arbres-Microorganismes, 54280, Champenoux, France.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31765287</idno><idno type="pmid">31765287</idno><idno type="doi">10.1094/MPMI-07-19-0208-R</idno><idno type="wicri:Area/Main/Corpus">000025</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000025</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Transcriptome Analysis of Apple Leaves Infected by the Rust Fungus <i>Gymnosporangium yamadae</i> at Two Sporulation Stages.</title><author><name sortKey="Tao, Si Qi" sort="Tao, Si Qi" uniqKey="Tao S" first="Si-Qi" last="Tao">Si-Qi Tao</name><affiliation><nlm:affiliation>The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Université de Lorraine, INRAE, Unité Mixte de Recherche 1136 Interactions Arbres-Microorganismes, 54280, Champenoux, France.</nlm:affiliation></affiliation></author><author><name sortKey="Auer, Lucas" sort="Auer, Lucas" uniqKey="Auer L" first="Lucas" last="Auer">Lucas Auer</name><affiliation><nlm:affiliation>Université de Lorraine, INRAE, Unité Mixte de Recherche 1136 Interactions Arbres-Microorganismes, 54280, Champenoux, France.</nlm:affiliation></affiliation></author><author><name sortKey="Morin, Emmanuelle" sort="Morin, Emmanuelle" uniqKey="Morin E" first="Emmanuelle" last="Morin">Emmanuelle Morin</name><affiliation><nlm:affiliation>Université de Lorraine, INRAE, Unité Mixte de Recherche 1136 Interactions Arbres-Microorganismes, 54280, Champenoux, France.</nlm:affiliation></affiliation></author><author><name sortKey="Liang, Ying Mei" sort="Liang, Ying Mei" uniqKey="Liang Y" first="Ying-Mei" last="Liang">Ying-Mei Liang</name><affiliation><nlm:affiliation>Museum of Beijing Forestry University, Beijing Forestry University.</nlm:affiliation></affiliation></author><author><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><affiliation><nlm:affiliation>Université de Lorraine, INRAE, Unité Mixte de Recherche 1136 Interactions Arbres-Microorganismes, 54280, Champenoux, France.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (pathogenicity)</term><term>Gene Expression Profiling (MeSH)</term><term>Malus (metabolism)</term><term>Malus (microbiology)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (metabolism)</term><term>Plant Leaves (microbiology)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Malus</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Malus</term><term>Plant Diseases</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term><term>Transcriptome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Apple rust disease caused by <i>Gymnosporangium yamadae</i> is one of the major threats to apple orchards. In this study, dual RNA-seq analysis was conducted to simultaneously monitor gene expression profiles of <i>G. yamadae</i> and infected apple leaves during the formation of rust spermogonia and aecia. The molecular mechanisms underlying this compatible interaction at 10 and 30 days postinoculation (dpi) indicate a significant reaction from the host plant and comprise detoxication pathways at the earliest stage and the induction of secondary metabolism pathways at 30 dpi. Such host reactions have been previously reported in other rust pathosystems and may represent a general reaction to rust infection. <i>G. yamadae</i> transcript profiling indicates a conserved genetic program in spermogonia and aecia that is shared with other rust fungi, whereas secretome prediction reveals the presence of specific secreted candidate effector proteins expressed during apple infection. Unexpectedly, the survey of fungal unigenes in the transcriptome assemblies of inoculated and mock-inoculated apple leaves reveals that <i>G. yamadae</i> infection may modify the fungal community composition in the apple phyllosphere at 30 dpi. Collectively, our results provide novel insights into the compatible apple-<i>G. yamadae</i> interaction and advance the knowledge of this heteroecious demicyclic rust fungus.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">31765287</PMID><DateCompleted><Year>2020</Year><Month>07</Month><Day>17</Day></DateCompleted><DateRevised><Year>2020</Year><Month>07</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>33</Volume><Issue>3</Issue><PubDate><Year>2020</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Transcriptome Analysis of Apple Leaves Infected by the Rust Fungus <i>Gymnosporangium yamadae</i> at Two Sporulation Stages.</ArticleTitle><Pagination><MedlinePgn>444-461</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-07-19-0208-R</ELocationID><Abstract><AbstractText>Apple rust disease caused by <i>Gymnosporangium yamadae</i> is one of the major threats to apple orchards. In this study, dual RNA-seq analysis was conducted to simultaneously monitor gene expression profiles of <i>G. yamadae</i> and infected apple leaves during the formation of rust spermogonia and aecia. The molecular mechanisms underlying this compatible interaction at 10 and 30 days postinoculation (dpi) indicate a significant reaction from the host plant and comprise detoxication pathways at the earliest stage and the induction of secondary metabolism pathways at 30 dpi. Such host reactions have been previously reported in other rust pathosystems and may represent a general reaction to rust infection. <i>G. yamadae</i> transcript profiling indicates a conserved genetic program in spermogonia and aecia that is shared with other rust fungi, whereas secretome prediction reveals the presence of specific secreted candidate effector proteins expressed during apple infection. Unexpectedly, the survey of fungal unigenes in the transcriptome assemblies of inoculated and mock-inoculated apple leaves reveals that <i>G. yamadae</i> infection may modify the fungal community composition in the apple phyllosphere at 30 dpi. Collectively, our results provide novel insights into the compatible apple-<i>G. yamadae</i> interaction and advance the knowledge of this heteroecious demicyclic rust fungus.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tao</LastName><ForeName>Si-Qi</ForeName><Initials>SQ</Initials><AffiliationInfo><Affiliation>The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Lorraine, INRAE, Unité Mixte de Recherche 1136 Interactions Arbres-Microorganismes, 54280, Champenoux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Auer</LastName><ForeName>Lucas</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Université de Lorraine, INRAE, Unité Mixte de Recherche 1136 Interactions Arbres-Microorganismes, 54280, Champenoux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morin</LastName><ForeName>Emmanuelle</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Université de Lorraine, INRAE, Unité Mixte de Recherche 1136 Interactions Arbres-Microorganismes, 54280, Champenoux, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Ying-Mei</ForeName><Initials>YM</Initials><AffiliationInfo><Affiliation>Museum of Beijing Forestry University, Beijing Forestry University.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Duplessis</LastName><ForeName>Sébastien</ForeName><Initials>S</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-2072-2989</Identifier><AffiliationInfo><Affiliation>Université de Lorraine, INRAE, Unité Mixte de Recherche 1136 Interactions Arbres-Microorganismes, 54280, Champenoux, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>01</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D027845" MajorTopicYN="N">Malus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="Y">Transcriptome</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Pucciniales</Keyword><Keyword MajorTopicYN="N">dual RNA-seq</Keyword><Keyword MajorTopicYN="N">obligate biotrophy</Keyword><Keyword MajorTopicYN="N">phyllosphere microbiome</Keyword><Keyword MajorTopicYN="N">plant secondary metabolism</Keyword><Keyword MajorTopicYN="N">secreted proteins</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>11</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>11</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31765287</ArticleId><ArticleId IdType="doi">10.1094/MPMI-07-19-0208-R</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RustEffectorV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000025 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000025 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RustEffectorV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:31765287
|texte= Transcriptome Analysis of Apple Leaves Infected by the Rust Fungus Gymnosporangium yamadae at Two Sporulation Stages.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:31765287" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a RustEffectorV1
| This area was generated with Dilib version V0.6.37. |  |