Measurement of mass, density, and volume during the cell cycle of yeast.
Identifieur interne : 001416 ( Main/Exploration ); précédent : 001415; suivant : 001417Measurement of mass, density, and volume during the cell cycle of yeast.
Auteurs : Andrea K. Bryan [États-Unis] ; Alexi Goranov ; Angelika Amon ; Scott R. ManalisSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2010.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Levures.
- cytologie : Levures.
- métabolisme : Actines, Levures.
- Cycle cellulaire, Cytométrie en flux.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Actins.
- cytology : Yeasts.
- growth & development : Yeasts.
- metabolism : Yeasts.
- Cell Cycle, Flow Cytometry.
Abstract
Cell growth comprises changes in both mass and volume--two processes that are distinct, yet coordinated through the cell cycle. Understanding this relationship requires a means for measuring each of the cell's three basic physical parameters: mass, volume, and the ratio of the two, density. The suspended microchannel resonator weighs single cells with a precision in mass of 0.1% for yeast. Here we use the suspended microchannel resonator with a Coulter counter to measure the mass, volume, and density of budding yeast cells through the cell cycle. We observe that cell density increases prior to bud formation at the G1/S transition, which is consistent with previous measurements using density gradient centrifugation. To investigate the origin of this density increase, we monitor relative density changes of growing yeast cells. We find that the density increase requires energy, function of the protein synthesis regulator target of rapamycin, passage through START (commitment to cell division), and an intact actin cytoskeleton. Although we focus on basic cell cycle questions in yeast, our techniques are suitable for most nonadherent cells and subcellular particles to characterize cell growth in a variety of applications.
DOI: 10.1073/pnas.0901851107
PubMed: 20080562
PubMed Central: PMC2824314
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Understanding this relationship requires a means for measuring each of the cell's three basic physical parameters: mass, volume, and the ratio of the two, density. The suspended microchannel resonator weighs single cells with a precision in mass of 0.1% for yeast. Here we use the suspended microchannel resonator with a Coulter counter to measure the mass, volume, and density of budding yeast cells through the cell cycle. We observe that cell density increases prior to bud formation at the G1/S transition, which is consistent with previous measurements using density gradient centrifugation. To investigate the origin of this density increase, we monitor relative density changes of growing yeast cells. We find that the density increase requires energy, function of the protein synthesis regulator target of rapamycin, passage through START (commitment to cell division), and an intact actin cytoskeleton. Although we focus on basic cell cycle questions in yeast, our techniques are suitable for most nonadherent cells and subcellular particles to characterize cell growth in a variety of applications.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20080562</PMID><DateCompleted><Year>2010</Year><Month>03</Month><Day>26</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1091-6490</ISSN><JournalIssue CitedMedium="Internet"><Volume>107</Volume><Issue>3</Issue><PubDate><Year>2010</Year><Month>Jan</Month><Day>19</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc Natl Acad Sci U S A</ISOAbbreviation></Journal><ArticleTitle>Measurement of mass, density, and volume during the cell cycle of yeast.</ArticleTitle><Pagination><MedlinePgn>999-1004</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1073/pnas.0901851107</ELocationID><Abstract><AbstractText>Cell growth comprises changes in both mass and volume--two processes that are distinct, yet coordinated through the cell cycle. Understanding this relationship requires a means for measuring each of the cell's three basic physical parameters: mass, volume, and the ratio of the two, density. The suspended microchannel resonator weighs single cells with a precision in mass of 0.1% for yeast. Here we use the suspended microchannel resonator with a Coulter counter to measure the mass, volume, and density of budding yeast cells through the cell cycle. We observe that cell density increases prior to bud formation at the G1/S transition, which is consistent with previous measurements using density gradient centrifugation. To investigate the origin of this density increase, we monitor relative density changes of growing yeast cells. We find that the density increase requires energy, function of the protein synthesis regulator target of rapamycin, passage through START (commitment to cell division), and an intact actin cytoskeleton. 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Manalis</name></noCountry><country name="États-Unis"><region name="Massachusetts"><name sortKey="Bryan, Andrea K" sort="Bryan, Andrea K" uniqKey="Bryan A" first="Andrea K" last="Bryan">Andrea K. Bryan</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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