RapamycinFungusV1, Main, Exploration, bibRecord, 000720

The Gcn4 transcription factor reduces protein synthesis capacity and extends yeast lifespan.

Identifieur interne : 000720 ( Main/Exploration ); précédent : 000719; suivant : 000721

The Gcn4 transcription factor reduces protein synthesis capacity and extends yeast lifespan.

Auteurs : Nitish Mittal [Suisse] ; Joao C. Guimaraes [Suisse] ; Thomas Gross [Suisse] ; Alexander Schmidt [Suisse] ; Arnau Vina-Vilaseca [Suisse] ; Danny D. Nedialkova [Allemagne] ; Florian Aeschimann [Suisse] ; Sebastian A. Leidel [Allemagne] ; Anne Spang [Suisse] ; Mihaela Zavolan [Suisse]

Source :

Nature communications [ 2041-1723 ] ; 2017.

RBID : pubmed:28878244

Descripteurs français

English descriptors

KwdEn :
- Basic-Leucine Zipper Transcription Factors (metabolism), Gene Expression Regulation, Fungal (MeSH), Genes, Fungal (MeSH), Open Reading Frames (genetics), Protein Biosynthesis (MeSH), Ribosomes (metabolism), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (metabolism), Saccharomyces cerevisiae (physiology), Saccharomyces cerevisiae Proteins (metabolism), Up-Regulation (genetics).
MESH :
- chemical , metabolism : Basic-Leucine Zipper Transcription Factors, Saccharomyces cerevisiae Proteins.
- genetics : Open Reading Frames, Saccharomyces cerevisiae, Up-Regulation.
- metabolism : Ribosomes, Saccharomyces cerevisiae.
- physiology : Saccharomyces cerevisiae.
- Gene Expression Regulation, Fungal, Genes, Fungal, Protein Biosynthesis.

Abstract

In Saccharomyces cerevisiae, deletion of large ribosomal subunit protein-encoding genes increases the replicative lifespan in a Gcn4-dependent manner. However, how Gcn4, a key transcriptional activator of amino acid biosynthesis genes, increases lifespan, is unknown. Here we show that Gcn4 acts as a repressor of protein synthesis. By analyzing the messenger RNA and protein abundance, ribosome occupancy and protein synthesis rate in various yeast strains, we demonstrate that Gcn4 is sufficient to reduce protein synthesis and increase yeast lifespan. Chromatin immunoprecipitation reveals Gcn4 binding not only at genes that are activated, but also at genes, some encoding ribosomal proteins, that are repressed upon Gcn4 overexpression. The promoters of repressed genes contain Rap1 binding motifs. Our data suggest that Gcn4 is a central regulator of protein synthesis under multiple perturbations, including ribosomal protein gene deletions, calorie restriction, and rapamycin treatment, and provide an explanation for its role in longevity and stress response.The transcription factor Gcn4 is known to regulate yeast amino acid synthesis. Here, the authors show that Gcn4 also acts as a repressor of protein biosynthesis in a range of conditions that enhance yeast lifespan, such as ribosomal protein knockout, calorie restriction or mTOR inhibition.

DOI: 10.1038/s41467-017-00539-y
PubMed: 28878244
PubMed Central: PMC5587724

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000737
to stream Main, to step Curation: 000737

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Gcn4 transcription factor reduces protein synthesis capacity and extends yeast lifespan.</title>
<author><name sortKey="Mittal, Nitish" sort="Mittal, Nitish" uniqKey="Mittal N" first="Nitish" last="Mittal">Nitish Mittal</name>
<affiliation wicri:level="1"><nlm:affiliation>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland. nitish.mittal@unibas.ch.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Guimaraes, Joao C" sort="Guimaraes, Joao C" uniqKey="Guimaraes J" first="Joao C" last="Guimaraes">Joao C. Guimaraes</name>
<affiliation wicri:level="1"><nlm:affiliation>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Gross, Thomas" sort="Gross, Thomas" uniqKey="Gross T" first="Thomas" last="Gross">Thomas Gross</name>
<affiliation wicri:level="1"><nlm:affiliation>Growth and Development, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Growth and Development, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Schmidt, Alexander" sort="Schmidt, Alexander" uniqKey="Schmidt A" first="Alexander" last="Schmidt">Alexander Schmidt</name>
<affiliation wicri:level="1"><nlm:affiliation>Proteomics Core Facility, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Proteomics Core Facility, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Vina Vilaseca, Arnau" sort="Vina Vilaseca, Arnau" uniqKey="Vina Vilaseca A" first="Arnau" last="Vina-Vilaseca">Arnau Vina-Vilaseca</name>
<affiliation wicri:level="1"><nlm:affiliation>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nedialkova, Danny D" sort="Nedialkova, Danny D" uniqKey="Nedialkova D" first="Danny D" last="Nedialkova">Danny D. Nedialkova</name>
<affiliation wicri:level="3"><nlm:affiliation>Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149, Münster, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149, Münster</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Münster</region>
<settlement type="city">Münster</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Aeschimann, Florian" sort="Aeschimann, Florian" uniqKey="Aeschimann F" first="Florian" last="Aeschimann">Florian Aeschimann</name>
<affiliation wicri:level="1"><nlm:affiliation>Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4002, Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4002, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Leidel, Sebastian A" sort="Leidel, Sebastian A" uniqKey="Leidel S" first="Sebastian A" last="Leidel">Sebastian A. Leidel</name>
<affiliation wicri:level="3"><nlm:affiliation>Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149, Münster, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149, Münster</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Münster</region>
<settlement type="city">Münster</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:affiliation>Cells-in-Motion Cluster of Excellence, University of Muenster, 48149, Muenster, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Cells-in-Motion Cluster of Excellence, University of Muenster, 48149, Muenster</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Münster</region>
<settlement type="city">Münster</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:affiliation>Faculty of Medicine, University of Muenster, Albert-Schweitzer-Campus 1, 48149, Muenster, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Faculty of Medicine, University of Muenster, Albert-Schweitzer-Campus 1, 48149, Muenster</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Münster</region>
<settlement type="city">Münster</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Spang, Anne" sort="Spang, Anne" uniqKey="Spang A" first="Anne" last="Spang">Anne Spang</name>
<affiliation wicri:level="1"><nlm:affiliation>Growth and Development, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Growth and Development, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zavolan, Mihaela" sort="Zavolan, Mihaela" uniqKey="Zavolan M" first="Mihaela" last="Zavolan">Mihaela Zavolan</name>
<affiliation wicri:level="1"><nlm:affiliation>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland. mihaela.zavolan@unibas.ch.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2017">2017</date>
<idno type="RBID">pubmed:28878244</idno>
<idno type="pmid">28878244</idno>
<idno type="doi">10.1038/s41467-017-00539-y</idno>
<idno type="pmc">PMC5587724</idno>
<idno type="wicri:Area/Main/Corpus">000737</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000737</idno>
<idno type="wicri:Area/Main/Curation">000737</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000737</idno>
<idno type="wicri:Area/Main/Exploration">000737</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">The Gcn4 transcription factor reduces protein synthesis capacity and extends yeast lifespan.</title>
<author><name sortKey="Mittal, Nitish" sort="Mittal, Nitish" uniqKey="Mittal N" first="Nitish" last="Mittal">Nitish Mittal</name>
<affiliation wicri:level="1"><nlm:affiliation>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland. nitish.mittal@unibas.ch.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Guimaraes, Joao C" sort="Guimaraes, Joao C" uniqKey="Guimaraes J" first="Joao C" last="Guimaraes">Joao C. Guimaraes</name>
<affiliation wicri:level="1"><nlm:affiliation>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Gross, Thomas" sort="Gross, Thomas" uniqKey="Gross T" first="Thomas" last="Gross">Thomas Gross</name>
<affiliation wicri:level="1"><nlm:affiliation>Growth and Development, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Growth and Development, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Schmidt, Alexander" sort="Schmidt, Alexander" uniqKey="Schmidt A" first="Alexander" last="Schmidt">Alexander Schmidt</name>
<affiliation wicri:level="1"><nlm:affiliation>Proteomics Core Facility, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Proteomics Core Facility, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Vina Vilaseca, Arnau" sort="Vina Vilaseca, Arnau" uniqKey="Vina Vilaseca A" first="Arnau" last="Vina-Vilaseca">Arnau Vina-Vilaseca</name>
<affiliation wicri:level="1"><nlm:affiliation>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nedialkova, Danny D" sort="Nedialkova, Danny D" uniqKey="Nedialkova D" first="Danny D" last="Nedialkova">Danny D. Nedialkova</name>
<affiliation wicri:level="3"><nlm:affiliation>Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149, Münster, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149, Münster</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Münster</region>
<settlement type="city">Münster</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Aeschimann, Florian" sort="Aeschimann, Florian" uniqKey="Aeschimann F" first="Florian" last="Aeschimann">Florian Aeschimann</name>
<affiliation wicri:level="1"><nlm:affiliation>Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4002, Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4002, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Leidel, Sebastian A" sort="Leidel, Sebastian A" uniqKey="Leidel S" first="Sebastian A" last="Leidel">Sebastian A. Leidel</name>
<affiliation wicri:level="3"><nlm:affiliation>Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149, Münster, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149, Münster</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Münster</region>
<settlement type="city">Münster</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:affiliation>Cells-in-Motion Cluster of Excellence, University of Muenster, 48149, Muenster, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Cells-in-Motion Cluster of Excellence, University of Muenster, 48149, Muenster</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Münster</region>
<settlement type="city">Münster</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:affiliation>Faculty of Medicine, University of Muenster, Albert-Schweitzer-Campus 1, 48149, Muenster, Germany.</nlm:affiliation>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Faculty of Medicine, University of Muenster, Albert-Schweitzer-Campus 1, 48149, Muenster</wicri:regionArea>
<placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region>
<region type="district" nuts="2">District de Münster</region>
<settlement type="city">Münster</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Spang, Anne" sort="Spang, Anne" uniqKey="Spang A" first="Anne" last="Spang">Anne Spang</name>
<affiliation wicri:level="1"><nlm:affiliation>Growth and Development, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Growth and Development, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zavolan, Mihaela" sort="Zavolan, Mihaela" uniqKey="Zavolan M" first="Mihaela" last="Zavolan">Mihaela Zavolan</name>
<affiliation wicri:level="1"><nlm:affiliation>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland. mihaela.zavolan@unibas.ch.</nlm:affiliation>
<country xml:lang="fr">Suisse</country>
<wicri:regionArea>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel</wicri:regionArea>
<wicri:noRegion>Basel</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Nature communications</title>
<idno type="eISSN">2041-1723</idno>
<imprint><date when="2017" type="published">2017</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basic-Leucine Zipper Transcription Factors (metabolism)</term>
<term>Gene Expression Regulation, Fungal (MeSH)</term>
<term>Genes, Fungal (MeSH)</term>
<term>Open Reading Frames (genetics)</term>
<term>Protein Biosynthesis (MeSH)</term>
<term>Ribosomes (metabolism)</term>
<term>Saccharomyces cerevisiae (genetics)</term>
<term>Saccharomyces cerevisiae (metabolism)</term>
<term>Saccharomyces cerevisiae (physiology)</term>
<term>Saccharomyces cerevisiae Proteins (metabolism)</term>
<term>Up-Regulation (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Biosynthèse des protéines (MeSH)</term>
<term>Cadres ouverts de lecture (génétique)</term>
<term>Facteurs de transcription à motif basique et à glissière à leucines (métabolisme)</term>
<term>Gènes fongiques (MeSH)</term>
<term>Protéines de Saccharomyces cerevisiae (métabolisme)</term>
<term>Ribosomes (métabolisme)</term>
<term>Régulation de l'expression des gènes fongiques (MeSH)</term>
<term>Régulation positive (génétique)</term>
<term>Saccharomyces cerevisiae (génétique)</term>
<term>Saccharomyces cerevisiae (métabolisme)</term>
<term>Saccharomyces cerevisiae (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Basic-Leucine Zipper Transcription Factors</term>
<term>Saccharomyces cerevisiae Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Open Reading Frames</term>
<term>Saccharomyces cerevisiae</term>
<term>Up-Regulation</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cadres ouverts de lecture</term>
<term>Régulation positive</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Ribosomes</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteurs de transcription à motif basique et à glissière à leucines</term>
<term>Protéines de Saccharomyces cerevisiae</term>
<term>Ribosomes</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Fungal</term>
<term>Genes, Fungal</term>
<term>Protein Biosynthesis</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Biosynthèse des protéines</term>
<term>Gènes fongiques</term>
<term>Régulation de l'expression des gènes fongiques</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">In Saccharomyces cerevisiae, deletion of large ribosomal subunit protein-encoding genes increases the replicative lifespan in a Gcn4-dependent manner. However, how Gcn4, a key transcriptional activator of amino acid biosynthesis genes, increases lifespan, is unknown. Here we show that Gcn4 acts as a repressor of protein synthesis. By analyzing the messenger RNA and protein abundance, ribosome occupancy and protein synthesis rate in various yeast strains, we demonstrate that Gcn4 is sufficient to reduce protein synthesis and increase yeast lifespan. Chromatin immunoprecipitation reveals Gcn4 binding not only at genes that are activated, but also at genes, some encoding ribosomal proteins, that are repressed upon Gcn4 overexpression. The promoters of repressed genes contain Rap1 binding motifs. Our data suggest that Gcn4 is a central regulator of protein synthesis under multiple perturbations, including ribosomal protein gene deletions, calorie restriction, and rapamycin treatment, and provide an explanation for its role in longevity and stress response.The transcription factor Gcn4 is known to regulate yeast amino acid synthesis. Here, the authors show that Gcn4 also acts as a repressor of protein biosynthesis in a range of conditions that enhance yeast lifespan, such as ribosomal protein knockout, calorie restriction or mTOR inhibition.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28878244</PMID>
<DateCompleted><Year>2018</Year>
<Month>01</Month>
<Day>08</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>01</Month>
<Day>16</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2041-1723</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>8</Volume>
<Issue>1</Issue>
<PubDate><Year>2017</Year>
<Month>09</Month>
<Day>06</Day>
</PubDate>
</JournalIssue>
<Title>Nature communications</Title>
<ISOAbbreviation>Nat Commun</ISOAbbreviation>
</Journal>
<ArticleTitle>The Gcn4 transcription factor reduces protein synthesis capacity and extends yeast lifespan.</ArticleTitle>
<Pagination><MedlinePgn>457</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1038/s41467-017-00539-y</ELocationID>
<Abstract><AbstractText>In Saccharomyces cerevisiae, deletion of large ribosomal subunit protein-encoding genes increases the replicative lifespan in a Gcn4-dependent manner. However, how Gcn4, a key transcriptional activator of amino acid biosynthesis genes, increases lifespan, is unknown. Here we show that Gcn4 acts as a repressor of protein synthesis. By analyzing the messenger RNA and protein abundance, ribosome occupancy and protein synthesis rate in various yeast strains, we demonstrate that Gcn4 is sufficient to reduce protein synthesis and increase yeast lifespan. Chromatin immunoprecipitation reveals Gcn4 binding not only at genes that are activated, but also at genes, some encoding ribosomal proteins, that are repressed upon Gcn4 overexpression. The promoters of repressed genes contain Rap1 binding motifs. Our data suggest that Gcn4 is a central regulator of protein synthesis under multiple perturbations, including ribosomal protein gene deletions, calorie restriction, and rapamycin treatment, and provide an explanation for its role in longevity and stress response.The transcription factor Gcn4 is known to regulate yeast amino acid synthesis. Here, the authors show that Gcn4 also acts as a repressor of protein biosynthesis in a range of conditions that enhance yeast lifespan, such as ribosomal protein knockout, calorie restriction or mTOR inhibition.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mittal</LastName>
<ForeName>Nitish</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland. nitish.mittal@unibas.ch.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Guimaraes</LastName>
<ForeName>Joao C</ForeName>
<Initials>JC</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0002-1664-472X</Identifier>
<AffiliationInfo><Affiliation>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Gross</LastName>
<ForeName>Thomas</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Growth and Development, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Schmidt</LastName>
<ForeName>Alexander</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Proteomics Core Facility, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Vina-Vilaseca</LastName>
<ForeName>Arnau</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nedialkova</LastName>
<ForeName>Danny D</ForeName>
<Initials>DD</Initials>
<AffiliationInfo><Affiliation>Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149, Münster, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Aeschimann</LastName>
<ForeName>Florian</ForeName>
<Initials>F</Initials>
<AffiliationInfo><Affiliation>Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4002, Basel, Switzerland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Leidel</LastName>
<ForeName>Sebastian A</ForeName>
<Initials>SA</Initials>
<AffiliationInfo><Affiliation>Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, Von-Esmarch-Strasse 54, 48149, Münster, Germany.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Cells-in-Motion Cluster of Excellence, University of Muenster, 48149, Muenster, Germany.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Faculty of Medicine, University of Muenster, Albert-Schweitzer-Campus 1, 48149, Muenster, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Spang</LastName>
<ForeName>Anne</ForeName>
<Initials>A</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0002-2387-6203</Identifier>
<AffiliationInfo><Affiliation>Growth and Development, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zavolan</LastName>
<ForeName>Mihaela</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Computational and Systems Biology, Biozentrum, University of Basel, Klingelbergstrasse 50-70, 4056, Basel, Switzerland. mihaela.zavolan@unibas.ch.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2017</Year>
<Month>09</Month>
<Day>06</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Nat Commun</MedlineTA>
<NlmUniqueID>101528555</NlmUniqueID>
<ISSNLinking>2041-1723</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D050976">Basic-Leucine Zipper Transcription Factors</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C501391">GCN4 protein, S cerevisiae</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D029701">Saccharomyces cerevisiae Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D050976" MajorTopicYN="N">Basic-Leucine Zipper Transcription Factors</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005800" MajorTopicYN="N">Genes, Fungal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016366" MajorTopicYN="N">Open Reading Frames</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014176" MajorTopicYN="Y">Protein Biosynthesis</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012270" MajorTopicYN="N">Ribosomes</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D029701" MajorTopicYN="N">Saccharomyces cerevisiae Proteins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015854" MajorTopicYN="N">Up-Regulation</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year>
<Month>11</Month>
<Day>02</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2017</Year>
<Month>07</Month>
<Day>07</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>9</Month>
<Day>8</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>9</Month>
<Day>8</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2018</Year>
<Month>1</Month>
<Day>9</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">28878244</ArticleId>
<ArticleId IdType="doi">10.1038/s41467-017-00539-y</ArticleId>
<ArticleId IdType="pii">10.1038/s41467-017-00539-y</ArticleId>
<ArticleId IdType="pmc">PMC5587724</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Nat Rev Genet. 2014 Dec;15(12 ):829-45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25365966</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Syst Biol. 2011 Nov 08;7:549</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22068332</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Mol Cell Biol. 2012 May 23;13(6):355-69</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22617470</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2013 Mar;8(3):539-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23429716</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 1991 Jun;11(6):3203-16</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2038326</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2009 Apr 10;324(5924):218-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19213877</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2011 Mar 2;30(5):859-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21183953</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Ageing Res Rev. 2011 Apr;10(2):225-37</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20385253</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2011 Dec 21;12(12):R125</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22189060</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Enzymol. 1991;194:3-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2005794</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell. 2011 Feb 18;41(4):480-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21329885</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Med. 2015 Dec;21(12):1416-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26646498</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2012 Jan;40(Database issue):D169-79</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22102575</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiology. 2002 Apr;148(Pt 4):1081-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11932453</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Biol. 2004 Aug;2(8):E246</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15314660</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Yeast. 1994 Dec;10(13):1793-808</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7747518</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genetics. 2011 Dec;189(4):1177-201</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22174183</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2005 Nov 18;310(5751):1193-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16293764</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Physiol Genomics. 2000 Aug 09;3(2):83-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11015603</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Wiley Interdiscip Rev RNA. 2016 Mar-Apr;7(2):198-212</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26732699</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 2007 Sep;27(17):5968-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17548477</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Cell. 2000 Dec;11(12):4241-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11102521</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 2000 Apr;20(8):2706-17</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10733573</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Genet. 2007 May;39(5):683-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17417638</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Aging Cell. 2007 Feb;6(1):111-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17266680</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2008 Jun 6;320(5881):1344-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18451266</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2016 Jan 29;351(6272):aad3867</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26823435</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11269-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15277680</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2015 Jun 18;161(7):1606-18</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26052047</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Adv Exp Med Biol. 2010;694:14-29</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20886753</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Bioinformatics. 2006 Mar 07;7:113</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16522208</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2004 Sep 2;431(7004):99-104</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15343339</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Yeast. 1998 Mar 30;14(5):471-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9559554</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Protoc. 2012 Jul 26;7(8):1534-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22836135</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Biotechnol. 2010 Sep;28(9):970-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20802496</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2009 Jul;37(Web Server issue):W202-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19458158</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1959 Jun 20;183(4677):1751-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">13666896</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2008;9(9):R137</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18798982</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Microbiol. 2005;59:407-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16153175</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genes Dev. 2005 Jul 1;19(13):1544-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15998808</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Metab. 2015 Nov 3;22(5):895-906</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26456335</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2008 Apr 18;133(2):292-302</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18423200</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Comput Biol. 2009 Sep;5(9):e1000502</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19750212</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Data Brief. 2015 Sep 04;5:297-304</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26550600</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Vis Exp. 2009 Jun 25;(28):null</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19556967</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Int Conf Intell Syst Mol Biol. 1994;2:28-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7584402</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Proteome Res. 2016 Aug 5;15(8):2537-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27345528</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Aging Cell. 2014 Dec;13(6):1012-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25156122</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Aging Cell. 2013 Feb;12(1):156-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23167605</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Exp Gerontol. 2007 Apr;42(4):275-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17174052</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 2001 Jul;21(13):4347-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11390663</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Cell Biol. 2013 May;15(5):481-90</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23624402</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2012 Apr 11;484(7393):251-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22498630</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Aging Cell. 2007 Feb;6(1):95-110</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17266679</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2010 Apr 16;328(5976):321-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20395504</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Commun. 2014 Nov 21;5:5465</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25413384</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2000 Oct 13;103(2):253-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11057898</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gerontol A Biol Sci Med Sci. 2015 Mar;70(3):263-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24691093</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2012 Feb 3;335(6068):552-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22194413</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Metab. 2010 Jun 9;11(6):453-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20519118</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Anal Chem. 2003 Sep 1;75(17):4646-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14632076</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 2015 Jan 29;160(3):477-88</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25619689</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Neurosci. 2013 Feb 6;33(6):2398-407</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23392669</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Bacteriol. 2001 Apr;183(7):2331-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11244074</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Genet. 2001 Aug;28(4):327-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11455386</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cell Biol. 2013 Dec;33(24):4811-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24100011</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Genet. 2007 Nov;8(11):835-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17909538</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Res. 2008 Apr;18(4):564-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18340043</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Yeast. 2005 Jan 15;22(1):1-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15565729</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Soc Trans. 2006 Feb;34(Pt 1):7-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16246168</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell. 1990 Jun 29;61(7):1199-208</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2163758</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1996 Jul 5;273(5271):54-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8658195</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2014;15(12):550</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25516281</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Allemagne</li>
<li>Suisse</li>
</country>
<region><li>District de Münster</li>
<li>Rhénanie-du-Nord-Westphalie</li>
</region>
<settlement><li>Münster</li>
</settlement>
</list>
<tree><country name="Suisse"><noRegion><name sortKey="Mittal, Nitish" sort="Mittal, Nitish" uniqKey="Mittal N" first="Nitish" last="Mittal">Nitish Mittal</name>
</noRegion>
<name sortKey="Aeschimann, Florian" sort="Aeschimann, Florian" uniqKey="Aeschimann F" first="Florian" last="Aeschimann">Florian Aeschimann</name>
<name sortKey="Gross, Thomas" sort="Gross, Thomas" uniqKey="Gross T" first="Thomas" last="Gross">Thomas Gross</name>
<name sortKey="Guimaraes, Joao C" sort="Guimaraes, Joao C" uniqKey="Guimaraes J" first="Joao C" last="Guimaraes">Joao C. Guimaraes</name>
<name sortKey="Schmidt, Alexander" sort="Schmidt, Alexander" uniqKey="Schmidt A" first="Alexander" last="Schmidt">Alexander Schmidt</name>
<name sortKey="Spang, Anne" sort="Spang, Anne" uniqKey="Spang A" first="Anne" last="Spang">Anne Spang</name>
<name sortKey="Vina Vilaseca, Arnau" sort="Vina Vilaseca, Arnau" uniqKey="Vina Vilaseca A" first="Arnau" last="Vina-Vilaseca">Arnau Vina-Vilaseca</name>
<name sortKey="Zavolan, Mihaela" sort="Zavolan, Mihaela" uniqKey="Zavolan M" first="Mihaela" last="Zavolan">Mihaela Zavolan</name>
</country>
<country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Nedialkova, Danny D" sort="Nedialkova, Danny D" uniqKey="Nedialkova D" first="Danny D" last="Nedialkova">Danny D. Nedialkova</name>
</region>
<name sortKey="Leidel, Sebastian A" sort="Leidel, Sebastian A" uniqKey="Leidel S" first="Sebastian A" last="Leidel">Sebastian A. Leidel</name>
<name sortKey="Leidel, Sebastian A" sort="Leidel, Sebastian A" uniqKey="Leidel S" first="Sebastian A" last="Leidel">Sebastian A. Leidel</name>
<name sortKey="Leidel, Sebastian A" sort="Leidel, Sebastian A" uniqKey="Leidel S" first="Sebastian A" last="Leidel">Sebastian A. Leidel</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RapamycinFungusV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000720 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000720 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    RapamycinFungusV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:28878244
   |texte=   The Gcn4 transcription factor reduces protein synthesis capacity and extends yeast lifespan.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:28878244" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a RapamycinFungusV1

This area was generated with Dilib version V0.6.38.
Data generation: Thu Nov 19 21:55:41 2020. Site generation: Thu Nov 19 22:00:39 2020

	Serveur d'exploration sur la rapamycine et les champignons
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur la rapamycine et les champignons

The Gcn4 transcription factor reduces protein synthesis capacity and extends yeast lifespan.

The Gcn4 transcription factor reduces protein synthesis capacity and extends yeast lifespan.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki