OrangerV1, PubMed, Corpus, bibRecord, 000413

Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.

Identifieur interne : 000413 ( PubMed/Corpus ); précédent : 000412; suivant : 000414

Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.

Auteurs : Marina M. Vdovenko ; Alexandra S. Stepanova ; Sergei A. Eremin ; Nguyen Van Cuong ; Natalia A. Uskova ; Ivan Yu Sakharov

Source :

Food chemistry [ 0308-8146 ] ; 2013.

RBID : pubmed:23790860

English descriptors

KwdEn :
- 2,4-Dichlorophenoxyacetic Acid (analysis), Citrus sinensis (chemistry), Drug Residues (analysis), Enzyme-Linked Immunosorbent Assay (methods), Fruit (chemistry), Herbicides (analysis), Limit of Detection, Luminescent Measurements (methods).
MESH :
- chemical , analysis : 2,4-Dichlorophenoxyacetic Acid, Herbicides.
- analysis : Drug Residues.
- chemistry : Citrus sinensis, Fruit.
- methods : Enzyme-Linked Immunosorbent Assay, Luminescent Measurements.
- Limit of Detection.

Abstract

Direct competitive enzyme-linked immunosorbent assay (ELISA) for 2,4-dichlorophenoxyacetic acid (2,4-D) was developed. Varying the concentrations of monoclonal anti-2,4-D-antibody and the conjugate of soybean peroxidase and 2,4-D the conditions of ELISA performance were optimised. The chemiluminescent method based on peroxidase-catalysed oxidation of luminol was applied to measure the enzyme activity of the conjugate. A mixture of 3-(10'-phenothiazinyl)propane-1-sulfonate and 4-morpholinopyridine was used as potent enhancer of chemiluminescence signal. It was shown that the values of the lower detection limit, IC50 and the working range were 1.5, 64.0, and 6.5-545ng/mL, respectively. The recovery values of CL-ELISA from 10 spiked samples of oranges (n=5) and mandarins (n=5) cultivated in green house without use of 2,4-D and containing different 2,4-D concentrations (10-300ng/mL) were ranged from 92% to 104% that indicated on the absence of matrix effect for the fruit extracts of interest. Determination of 2,4-D in peel of five oranges and five mandarins purchased from stores in Vietnam showed that 2,4-D content in oranges fruits (79-104μg/kg) was significantly higher than that in mandarins (1.66-2.82μg/kg).

DOI: 10.1016/j.foodchem.2013.04.060
PubMed: 23790860

Links to Exploration step

pubmed:23790860

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.</title>
<author><name sortKey="Vdovenko, Marina M" sort="Vdovenko, Marina M" uniqKey="Vdovenko M" first="Marina M" last="Vdovenko">Marina M. Vdovenko</name>
<affiliation><nlm:affiliation>Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Stepanova, Alexandra S" sort="Stepanova, Alexandra S" uniqKey="Stepanova A" first="Alexandra S" last="Stepanova">Alexandra S. Stepanova</name>
</author>
<author><name sortKey="Eremin, Sergei A" sort="Eremin, Sergei A" uniqKey="Eremin S" first="Sergei A" last="Eremin">Sergei A. Eremin</name>
</author>
<author><name sortKey="Van Cuong, Nguyen" sort="Van Cuong, Nguyen" uniqKey="Van Cuong N" first="Nguyen" last="Van Cuong">Nguyen Van Cuong</name>
</author>
<author><name sortKey="Uskova, Natalia A" sort="Uskova, Natalia A" uniqKey="Uskova N" first="Natalia A" last="Uskova">Natalia A. Uskova</name>
</author>
<author><name sortKey="Yu Sakharov, Ivan" sort="Yu Sakharov, Ivan" uniqKey="Yu Sakharov I" first="Ivan" last="Yu Sakharov">Ivan Yu Sakharov</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2013">2013</date>
<idno type="RBID">pubmed:23790860</idno>
<idno type="pmid">23790860</idno>
<idno type="doi">10.1016/j.foodchem.2013.04.060</idno>
<idno type="wicri:Area/PubMed/Corpus">000413</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.</title>
<author><name sortKey="Vdovenko, Marina M" sort="Vdovenko, Marina M" uniqKey="Vdovenko M" first="Marina M" last="Vdovenko">Marina M. Vdovenko</name>
<affiliation><nlm:affiliation>Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Stepanova, Alexandra S" sort="Stepanova, Alexandra S" uniqKey="Stepanova A" first="Alexandra S" last="Stepanova">Alexandra S. Stepanova</name>
</author>
<author><name sortKey="Eremin, Sergei A" sort="Eremin, Sergei A" uniqKey="Eremin S" first="Sergei A" last="Eremin">Sergei A. Eremin</name>
</author>
<author><name sortKey="Van Cuong, Nguyen" sort="Van Cuong, Nguyen" uniqKey="Van Cuong N" first="Nguyen" last="Van Cuong">Nguyen Van Cuong</name>
</author>
<author><name sortKey="Uskova, Natalia A" sort="Uskova, Natalia A" uniqKey="Uskova N" first="Natalia A" last="Uskova">Natalia A. Uskova</name>
</author>
<author><name sortKey="Yu Sakharov, Ivan" sort="Yu Sakharov, Ivan" uniqKey="Yu Sakharov I" first="Ivan" last="Yu Sakharov">Ivan Yu Sakharov</name>
</author>
</analytic>
<series><title level="j">Food chemistry</title>
<idno type="ISSN">0308-8146</idno>
<imprint><date when="2013" type="published">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>2,4-Dichlorophenoxyacetic Acid (analysis)</term>
<term>Citrus sinensis (chemistry)</term>
<term>Drug Residues (analysis)</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Fruit (chemistry)</term>
<term>Herbicides (analysis)</term>
<term>Limit of Detection</term>
<term>Luminescent Measurements (methods)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>2,4-Dichlorophenoxyacetic Acid</term>
<term>Herbicides</term>
</keywords>
<keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Drug Residues</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term>
<term>Fruit</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
<term>Luminescent Measurements</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Limit of Detection</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Direct competitive enzyme-linked immunosorbent assay (ELISA) for 2,4-dichlorophenoxyacetic acid (2,4-D) was developed. Varying the concentrations of monoclonal anti-2,4-D-antibody and the conjugate of soybean peroxidase and 2,4-D the conditions of ELISA performance were optimised. The chemiluminescent method based on peroxidase-catalysed oxidation of luminol was applied to measure the enzyme activity of the conjugate. A mixture of 3-(10'-phenothiazinyl)propane-1-sulfonate and 4-morpholinopyridine was used as potent enhancer of chemiluminescence signal. It was shown that the values of the lower detection limit, IC50 and the working range were 1.5, 64.0, and 6.5-545ng/mL, respectively. The recovery values of CL-ELISA from 10 spiked samples of oranges (n=5) and mandarins (n=5) cultivated in green house without use of 2,4-D and containing different 2,4-D concentrations (10-300ng/mL) were ranged from 92% to 104% that indicated on the absence of matrix effect for the fruit extracts of interest. Determination of 2,4-D in peel of five oranges and five mandarins purchased from stores in Vietnam showed that 2,4-D content in oranges fruits (79-104μg/kg) was significantly higher than that in mandarins (1.66-2.82μg/kg).</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23790860</PMID>
<DateCreated><Year>2013</Year>
<Month>6</Month>
<Day>24</Day>
</DateCreated>
<DateCompleted><Year>2014</Year>
<Month>01</Month>
<Day>03</Day>
</DateCompleted>
<DateRevised><Year>2013</Year>
<Month>6</Month>
<Day>24</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0308-8146</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>141</Volume>
<Issue>2</Issue>
<PubDate><Year>2013</Year>
<Month>Nov</Month>
<Day>15</Day>
</PubDate>
</JournalIssue>
<Title>Food chemistry</Title>
<ISOAbbreviation>Food Chem</ISOAbbreviation>
</Journal>
<ArticleTitle>Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.</ArticleTitle>
<Pagination><MedlinePgn>865-8</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.foodchem.2013.04.060</ELocationID>
<ELocationID EIdType="pii" ValidYN="Y">S0308-8146(13)00506-2</ELocationID>
<Abstract><AbstractText>Direct competitive enzyme-linked immunosorbent assay (ELISA) for 2,4-dichlorophenoxyacetic acid (2,4-D) was developed. Varying the concentrations of monoclonal anti-2,4-D-antibody and the conjugate of soybean peroxidase and 2,4-D the conditions of ELISA performance were optimised. The chemiluminescent method based on peroxidase-catalysed oxidation of luminol was applied to measure the enzyme activity of the conjugate. A mixture of 3-(10'-phenothiazinyl)propane-1-sulfonate and 4-morpholinopyridine was used as potent enhancer of chemiluminescence signal. It was shown that the values of the lower detection limit, IC50 and the working range were 1.5, 64.0, and 6.5-545ng/mL, respectively. The recovery values of CL-ELISA from 10 spiked samples of oranges (n=5) and mandarins (n=5) cultivated in green house without use of 2,4-D and containing different 2,4-D concentrations (10-300ng/mL) were ranged from 92% to 104% that indicated on the absence of matrix effect for the fruit extracts of interest. Determination of 2,4-D in peel of five oranges and five mandarins purchased from stores in Vietnam showed that 2,4-D content in oranges fruits (79-104μg/kg) was significantly higher than that in mandarins (1.66-2.82μg/kg).</AbstractText>
<CopyrightInformation>Copyright © 2013 Elsevier Ltd. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vdovenko</LastName>
<ForeName>Marina M</ForeName>
<Initials>MM</Initials>
<AffiliationInfo><Affiliation>Faculty of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Stepanova</LastName>
<ForeName>Alexandra S</ForeName>
<Initials>AS</Initials>
</Author>
<Author ValidYN="Y"><LastName>Eremin</LastName>
<ForeName>Sergei A</ForeName>
<Initials>SA</Initials>
</Author>
<Author ValidYN="Y"><LastName>Van Cuong</LastName>
<ForeName>Nguyen</ForeName>
<Initials>N</Initials>
</Author>
<Author ValidYN="Y"><LastName>Uskova</LastName>
<ForeName>Natalia A</ForeName>
<Initials>NA</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yu Sakharov</LastName>
<ForeName>Ivan</ForeName>
<Initials>I</Initials>
</Author>
</AuthorList>
<Language>ENG</Language>
<PublicationTypeList><PublicationType UI="D023362">Evaluation Studies</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2013</Year>
<Month>May</Month>
<Day>01</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Food Chem</MedlineTA>
<NlmUniqueID>7702639</NlmUniqueID>
<ISSNLinking>0308-8146</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D006540">Herbicides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>2577AQ9262</RegistryNumber>
<NameOfSubstance UI="D015084">2,4-Dichlorophenoxyacetic Acid</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D015084" MajorTopicYN="N">2,4-Dichlorophenoxyacetic Acid</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004350" MajorTopicYN="N">Drug Residues</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005638" MajorTopicYN="N">Fruit</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006540" MajorTopicYN="N">Herbicides</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057230" MajorTopicYN="N">Limit of Detection</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008163" MajorTopicYN="N">Luminescent Measurements</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year>
<Month>10</Month>
<Day>13</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2013</Year>
<Month>2</Month>
<Day>23</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2013</Year>
<Month>4</Month>
<Day>19</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2013</Year>
<Month>6</Month>
<Day>25</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2013</Year>
<Month>6</Month>
<Day>26</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2014</Year>
<Month>1</Month>
<Day>5</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">23790860</ArticleId>
<ArticleId IdType="pii">S0308-8146(13)00506-2</ArticleId>
<ArticleId IdType="doi">10.1016/j.foodchem.2013.04.060</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/PubMed/Corpus

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000413 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000413 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    PubMed
   |étape=   Corpus
   |type=    RBID
   |clé=     pubmed:23790860
   |texte=   Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i   -Sk "pubmed:23790860" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd   \
       | NlmPubMed2Wicri -a OrangerV1

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'oranger

Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.

Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki