Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices.
Identifieur interne : 000384 ( PubMed/Corpus ); précédent : 000383; suivant : 000385Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices.
Auteurs : Emma Plana ; Maria-José Moreno ; Ángel Montoya ; Juan J. ManclúsSource :
- Food chemistry [ 0308-8146 ] ; 2014.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (analysis), Antibodies, Monoclonal (genetics), Antibodies, Monoclonal (immunology), Beverages (analysis), Chlorobenzenes (analysis), Citrus sinensis (chemistry), Enzyme-Linked Immunosorbent Assay (methods), Food Contamination (analysis), Fruit (chemistry), Fungicides, Industrial (analysis), Malus (chemistry), Mice, Pesticide Residues (analysis), Triazoles (analysis).
- MESH :
- chemical , analysis : Antibodies, Monoclonal, Chlorobenzenes, Fungicides, Industrial, Pesticide Residues, Triazoles.
- chemical , genetics : Antibodies, Monoclonal.
- chemical , immunology : Antibodies, Monoclonal.
- analysis : Beverages, Food Contamination.
- chemistry : Citrus sinensis, Fruit, Malus.
- methods : Enzyme-Linked Immunosorbent Assay.
- Animals, Mice.
Abstract
Tetraconazole is currently used as a fungicide in fruit and vegetables. The aim of this work was the development of immunochemical techniques based on recombinant antibodies for the screening of tetraconazole residues in fruit juices. Recombinant antibodies were produced from a hybridoma cell line secreting a monoclonal antibody specific for tetraconazole and from lymphocytes of mice hyperimmunised with tetraconazole haptens conjugated to bovine serum albumin. From these antibodies, enzyme-linked immunosorbent assays in the conjugate-coated format were developed, which were able to detect tetraconazole standards down to 1ng/mL. From recovery studies with spiked samples, these immunoassays determined tetraconazole in orange and apple juices with acceptable reproducibility (coefficients of variation below 25%) and recoveries (ranging from 78% to 145%) for a screening technique. The analytical performance of RAb-based immunoassays was fairly similar to that of the MAb-based immunoassays. Due to their simplicity and high sample throughput, the developed recombinant-based immunoassays can be valuable analytical tools for the screening of tetraconazole residues in fruit juices at regulatory levels.
DOI: 10.1016/j.foodchem.2013.07.121
PubMed: 24054232
Links to Exploration step
pubmed:24054232Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices.</title><author><name sortKey="Plana, Emma" sort="Plana, Emma" uniqKey="Plana E" first="Emma" last="Plana">Emma Plana</name><affiliation><nlm:affiliation>Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano, Universitat Politècnica de València, Camino de Vera s/n, Edificio 8B-N, 46022 Valencia, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Moreno, Maria Jose" sort="Moreno, Maria Jose" uniqKey="Moreno M" first="Maria-José" last="Moreno">Maria-José Moreno</name></author><author><name sortKey="Montoya, Angel" sort="Montoya, Angel" uniqKey="Montoya A" first="Ángel" last="Montoya">Ángel Montoya</name></author><author><name sortKey="Manclus, Juan J" sort="Manclus, Juan J" uniqKey="Manclus J" first="Juan J" last="Manclús">Juan J. Manclús</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24054232</idno><idno type="pmid">24054232</idno><idno type="doi">10.1016/j.foodchem.2013.07.121</idno><idno type="wicri:Area/PubMed/Corpus">000384</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices.</title><author><name sortKey="Plana, Emma" sort="Plana, Emma" uniqKey="Plana E" first="Emma" last="Plana">Emma Plana</name><affiliation><nlm:affiliation>Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano, Universitat Politècnica de València, Camino de Vera s/n, Edificio 8B-N, 46022 Valencia, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Moreno, Maria Jose" sort="Moreno, Maria Jose" uniqKey="Moreno M" first="Maria-José" last="Moreno">Maria-José Moreno</name></author><author><name sortKey="Montoya, Angel" sort="Montoya, Angel" uniqKey="Montoya A" first="Ángel" last="Montoya">Ángel Montoya</name></author><author><name sortKey="Manclus, Juan J" sort="Manclus, Juan J" uniqKey="Manclus J" first="Juan J" last="Manclús">Juan J. Manclús</name></author></analytic><series><title level="j">Food chemistry</title><idno type="ISSN">0308-8146</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (analysis)</term><term>Antibodies, Monoclonal (genetics)</term><term>Antibodies, Monoclonal (immunology)</term><term>Beverages (analysis)</term><term>Chlorobenzenes (analysis)</term><term>Citrus sinensis (chemistry)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Food Contamination (analysis)</term><term>Fruit (chemistry)</term><term>Fungicides, Industrial (analysis)</term><term>Malus (chemistry)</term><term>Mice</term><term>Pesticide Residues (analysis)</term><term>Triazoles (analysis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Chlorobenzenes</term><term>Fungicides, Industrial</term><term>Pesticide Residues</term><term>Triazoles</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Beverages</term><term>Food Contamination</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term><term>Fruit</term><term>Malus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Mice</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Tetraconazole is currently used as a fungicide in fruit and vegetables. The aim of this work was the development of immunochemical techniques based on recombinant antibodies for the screening of tetraconazole residues in fruit juices. Recombinant antibodies were produced from a hybridoma cell line secreting a monoclonal antibody specific for tetraconazole and from lymphocytes of mice hyperimmunised with tetraconazole haptens conjugated to bovine serum albumin. From these antibodies, enzyme-linked immunosorbent assays in the conjugate-coated format were developed, which were able to detect tetraconazole standards down to 1ng/mL. From recovery studies with spiked samples, these immunoassays determined tetraconazole in orange and apple juices with acceptable reproducibility (coefficients of variation below 25%) and recoveries (ranging from 78% to 145%) for a screening technique. The analytical performance of RAb-based immunoassays was fairly similar to that of the MAb-based immunoassays. Due to their simplicity and high sample throughput, the developed recombinant-based immunoassays can be valuable analytical tools for the screening of tetraconazole residues in fruit juices at regulatory levels.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24054232</PMID><DateCreated><Year>2013</Year><Month>9</Month><Day>23</Day></DateCreated><DateCompleted><Year>2014</Year><Month>02</Month><Day>20</Day></DateCompleted><DateRevised><Year>2013</Year><Month>9</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0308-8146</ISSN><JournalIssue CitedMedium="Internet"><Volume>143</Volume><PubDate><Year>2014</Year><Month>Jan</Month><Day>15</Day></PubDate></JournalIssue><Title>Food chemistry</Title><ISOAbbreviation>Food Chem</ISOAbbreviation></Journal><ArticleTitle>Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices.</ArticleTitle><Pagination><MedlinePgn>205-13</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.foodchem.2013.07.121</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0308-8146(13)01050-9</ELocationID><Abstract><AbstractText>Tetraconazole is currently used as a fungicide in fruit and vegetables. The aim of this work was the development of immunochemical techniques based on recombinant antibodies for the screening of tetraconazole residues in fruit juices. Recombinant antibodies were produced from a hybridoma cell line secreting a monoclonal antibody specific for tetraconazole and from lymphocytes of mice hyperimmunised with tetraconazole haptens conjugated to bovine serum albumin. From these antibodies, enzyme-linked immunosorbent assays in the conjugate-coated format were developed, which were able to detect tetraconazole standards down to 1ng/mL. From recovery studies with spiked samples, these immunoassays determined tetraconazole in orange and apple juices with acceptable reproducibility (coefficients of variation below 25%) and recoveries (ranging from 78% to 145%) for a screening technique. The analytical performance of RAb-based immunoassays was fairly similar to that of the MAb-based immunoassays. Due to their simplicity and high sample throughput, the developed recombinant-based immunoassays can be valuable analytical tools for the screening of tetraconazole residues in fruit juices at regulatory levels.</AbstractText><CopyrightInformation>Copyright © 2013 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Plana</LastName><ForeName>Emma</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Instituto Interuniversitario de Investigación en Bioingeniería y Tecnología Orientada al Ser Humano, Universitat Politècnica de València, Camino de Vera s/n, Edificio 8B-N, 46022 Valencia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moreno</LastName><ForeName>Maria-José</ForeName><Initials>MJ</Initials></Author><Author ValidYN="Y"><LastName>Montoya</LastName><ForeName>Ángel</ForeName><Initials>Á</Initials></Author><Author ValidYN="Y"><LastName>Manclús</LastName><ForeName>Juan J</ForeName><Initials>JJ</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Studies</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>Aug</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Food Chem</MedlineTA><NlmUniqueID>7702639</NlmUniqueID><ISSNLinking>0308-8146</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002722">Chlorobenzenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005659">Fungicides, Industrial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010573">Pesticide Residues</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014230">Triazoles</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C524826">tetraconazole</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001628" MajorTopicYN="N">Beverages</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002722" MajorTopicYN="N">Chlorobenzenes</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005506" MajorTopicYN="N">Food Contamination</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005638" MajorTopicYN="N">Fruit</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005659" MajorTopicYN="N">Fungicides, Industrial</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027845" MajorTopicYN="N">Malus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010573" MajorTopicYN="N">Pesticide Residues</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014230" MajorTopicYN="N">Triazoles</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ELISA</Keyword><Keyword MajorTopicYN="N">Fruit juices</Keyword><Keyword MajorTopicYN="N">Fungicide residues</Keyword><Keyword MajorTopicYN="N">Immunochemical analysis</Keyword><Keyword MajorTopicYN="N">Recombinant antibodies</Keyword><Keyword MajorTopicYN="N">ScFv</Keyword><Keyword MajorTopicYN="N">Tetraconazole</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>7</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>1</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>7</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>9</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>9</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>2</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24054232</ArticleId><ArticleId IdType="pii">S0308-8146(13)01050-9</ArticleId><ArticleId IdType="doi">10.1016/j.foodchem.2013.07.121</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000384 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000384 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:24054232
|texte= Development and application of recombinant antibody-based immunoassays to tetraconazole residue analysis in fruit juices.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:24054232" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |