OrangerV1, PascalFrancis, Curation, bibRecord, 000147

Extraction parameters and capillary electrophoresis analysis of limonin glucoside and phlorin in citrus byproducts

Identifieur interne : 000147 ( PascalFrancis/Curation ); précédent : 000146; suivant : 000148

Extraction parameters and capillary electrophoresis analysis of limonin glucoside and phlorin in citrus byproducts

Auteurs : Robert J. Braddock [États-Unis] ; Charles R. Bryan [États-Unis]

Source :

Journal of agricultural and food chemistry : (Print) [ 0021-8561 ] ; 2001.

RBID : Pascal:02-0115223

Descripteurs français

Pascal (Inist)
- Procédé extraction, Méthode analyse, Electrophorèse capillaire, Sous produit, Pulpe fruit, Peau, Albédo, Purification, Solution aqueuse, Citrus paradisi, Citrus sinensis, Limonine, Méthanol, Ethanol, Polygalacturonase, Chromatographie HPLC, Valorisation, Phlorine, Limonine glucoside.
Wicri :
- topic : Méthanol.

English descriptors

KwdEn :
- Albedo, Analysis method, Aqueous solution, By product, Capillary electrophoresis, Citrus paradisi, Citrus sinensis, Ethanol, Extraction process, Fruit pulp, HPLC chromatography, Limonin, Methanol, Polygalacturonase, Purification, Skin, Upgrading.

Abstract

Limonin glucoside (LG) and phlorin were extracted from citrus fruit tissues and assayed by capillary electrophoresis (CE). LG was determined in dried [1.20 ± 0.10 mg of dry weight (dw)] and wet peel residues (1.16 ± 0.04 mg of dw), orange juice finisher pulp (0.58 ± 0.03 mg of dw), dried grapefruit seeds (2.70 ± 0.15 mg of dw), and 50 °Brix molasses (2225 ± 68 mg/L). Phlorin was purified from orange peel residue and grapefruit albedo, and concentrations were determined in some citrus products. Phlorin and LG were extracted from residues with water/pectinase or with water solutions of methanol and ethanol. Efficient LG extraction from grapefruit seeds (2.40 ± 0.15 mg/g) was achieved with 50-65% methanol, solvent polarity P' 7-8. Extracts were purified and concentrated by adsorptive resins and HPLC to obtain 95% pure compounds of LG and phlorin. CE analysis did not require extract purification beyond filtration. LG and phlorin migrated as anions in electropherograms containing peaks representing other citrus flavonoids and limonoid glucosides.

A01	`01`	`1`		`@0 0021-8561`
A02	`01`			`@0 JAFCAU`
A03		`1`		`@0 J. agric. food chem. : (Print)`
A05				`@2 49`
A06				`@2 12`
A08	`01`	`1`	`ENG`	`@1 Extraction parameters and capillary electrophoresis analysis of limonin glucoside and phlorin in citrus byproducts`
A11	`01`	`1`		`@1 BRADDOCK (Robert J.)`
A11	`02`	`1`		`@1 BRYAN (Charles R.)`
A14	`01`			`@1 Citrus Research and Education Center, University of Florida, 700 Experiment Station Road @2 Lake Alfred, Florida 33850 @3 USA @Z 1 aut. @Z 2 aut.`
A20				`@1 5982-5988`
A21				`@1 2001`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 7332 @5 354000094686210480`
A44				`@0 0000 @1 © 2002 INIST-CNRS. All rights reserved.`
A45				`@0 20 ref.`
A47	`01`	`1`		`@0 02-0115223`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Journal of agricultural and food chemistry : (Print)`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 Limonin glucoside (LG) and phlorin were extracted from citrus fruit tissues and assayed by capillary electrophoresis (CE). LG was determined in dried [1.20 ± 0.10 mg of dry weight (dw)] and wet peel residues (1.16 ± 0.04 mg of dw), orange juice finisher pulp (0.58 ± 0.03 mg of dw), dried grapefruit seeds (2.70 ± 0.15 mg of dw), and 50 °Brix molasses (2225 ± 68 mg/L). Phlorin was purified from orange peel residue and grapefruit albedo, and concentrations were determined in some citrus products. Phlorin and LG were extracted from residues with water/pectinase or with water solutions of methanol and ethanol. Efficient LG extraction from grapefruit seeds (2.40 ± 0.15 mg/g) was achieved with 50-65% methanol, solvent polarity P' 7-8. Extracts were purified and concentrated by adsorptive resins and HPLC to obtain 95% pure compounds of LG and phlorin. CE analysis did not require extract purification beyond filtration. LG and phlorin migrated as anions in electropherograms containing peaks representing other citrus flavonoids and limonoid glucosides.
C02	`01`	`X`		`@0 002A35F`
C03	`01`	`X`	`FRE`	`@0 Procédé extraction @5 01`
C03	`01`	`X`	`ENG`	`@0 Extraction process @5 01`
C03	`01`	`X`	`SPA`	`@0 Procedimiento extracción @5 01`
C03	`02`	`X`	`FRE`	`@0 Méthode analyse @5 02`
C03	`02`	`X`	`ENG`	`@0 Analysis method @5 02`
C03	`02`	`X`	`SPA`	`@0 Método análisis @5 02`
C03	`03`	`X`	`FRE`	`@0 Electrophorèse capillaire @5 03`
C03	`03`	`X`	`ENG`	`@0 Capillary electrophoresis @5 03`
C03	`03`	`X`	`SPA`	`@0 Electroforesis capilar @5 03`
C03	`04`	`X`	`FRE`	`@0 Sous produit @5 04`
C03	`04`	`X`	`ENG`	`@0 By product @5 04`
C03	`04`	`X`	`SPA`	`@0 Subproducto @5 04`
C03	`05`	`X`	`FRE`	`@0 Pulpe fruit @5 05`
C03	`05`	`X`	`ENG`	`@0 Fruit pulp @5 05`
C03	`05`	`X`	`SPA`	`@0 Pulpa fruto @5 05`
C03	`06`	`X`	`FRE`	`@0 Peau @5 06`
C03	`06`	`X`	`ENG`	`@0 Skin @5 06`
C03	`06`	`X`	`SPA`	`@0 Piel @5 06`
C03	`07`	`X`	`FRE`	`@0 Albédo @5 07`
C03	`07`	`X`	`ENG`	`@0 Albedo @5 07`
C03	`07`	`X`	`SPA`	`@0 Albedo @5 07`
C03	`08`	`X`	`FRE`	`@0 Purification @5 08`
C03	`08`	`X`	`ENG`	`@0 Purification @5 08`
C03	`08`	`X`	`SPA`	`@0 Purificación @5 08`
C03	`09`	`X`	`FRE`	`@0 Solution aqueuse @5 09`
C03	`09`	`X`	`ENG`	`@0 Aqueous solution @5 09`
C03	`09`	`X`	`SPA`	`@0 Solución acuosa @5 09`
C03	`10`	`X`	`FRE`	`@0 Citrus paradisi @2 NS @5 10`
C03	`10`	`X`	`ENG`	`@0 Citrus paradisi @2 NS @5 10`
C03	`10`	`X`	`SPA`	`@0 Citrus paradisi @2 NS @5 10`
C03	`11`	`X`	`FRE`	`@0 Citrus sinensis @2 NS @5 11`
C03	`11`	`X`	`ENG`	`@0 Citrus sinensis @2 NS @5 11`
C03	`11`	`X`	`SPA`	`@0 Citrus sinensis @2 NS @5 11`
C03	`12`	`X`	`FRE`	`@0 Limonine @5 15`
C03	`12`	`X`	`ENG`	`@0 Limonin @5 15`
C03	`12`	`X`	`SPA`	`@0 Limonina @5 15`
C03	`13`	`X`	`FRE`	`@0 Méthanol @2 NK @2 FX @5 16`
C03	`13`	`X`	`ENG`	`@0 Methanol @2 NK @2 FX @5 16`
C03	`13`	`X`	`SPA`	`@0 Metanol @2 NK @2 FX @5 16`
C03	`14`	`X`	`FRE`	`@0 Ethanol @2 NK @2 FR @2 FX @5 17`
C03	`14`	`X`	`ENG`	`@0 Ethanol @2 NK @2 FR @2 FX @5 17`
C03	`14`	`X`	`SPA`	`@0 Etanol @2 NK @2 FR @2 FX @5 17`
C03	`15`	`X`	`FRE`	`@0 Polygalacturonase @2 NK @2 FE @5 33`
C03	`15`	`X`	`ENG`	`@0 Polygalacturonase @2 NK @2 FE @5 33`
C03	`15`	`X`	`SPA`	`@0 Polygalacturonase @2 NK @2 FE @5 33`
C03	`16`	`X`	`FRE`	`@0 Chromatographie HPLC @5 34`
C03	`16`	`X`	`ENG`	`@0 HPLC chromatography @5 34`
C03	`16`	`X`	`SPA`	`@0 Cromatografía HPLC @5 34`
C03	`17`	`X`	`FRE`	`@0 Valorisation @5 35`
C03	`17`	`X`	`ENG`	`@0 Upgrading @5 35`
C03	`17`	`X`	`SPA`	`@0 Valorización @5 35`
C03	`18`	`X`	`FRE`	`@0 Phlorine @2 NK @4 INC @5 78`
C03	`19`	`X`	`FRE`	`@0 Limonine glucoside @2 NK @4 INC @5 79`
C07	`01`	`X`	`FRE`	`@0 Rutaceae @2 NS`
C07	`01`	`X`	`ENG`	`@0 Rutaceae @2 NS`
C07	`01`	`X`	`SPA`	`@0 Rutaceae @2 NS`
C07	`02`	`X`	`FRE`	`@0 Dicotyledones @2 NS`
C07	`02`	`X`	`ENG`	`@0 Dicotyledones @2 NS`
C07	`02`	`X`	`SPA`	`@0 Dicotyledones @2 NS`
C07	`03`	`X`	`FRE`	`@0 Angiospermae @2 NS`
C07	`03`	`X`	`ENG`	`@0 Angiospermae @2 NS`
C07	`03`	`X`	`SPA`	`@0 Angiospermae @2 NS`
C07	`04`	`X`	`FRE`	`@0 Spermatophyta @2 NS`
C07	`04`	`X`	`ENG`	`@0 Spermatophyta @2 NS`
C07	`04`	`X`	`SPA`	`@0 Spermatophyta @2 NS`
C07	`05`	`X`	`FRE`	`@0 O-Glycosidases @2 FE @6 «O»-Glycosidases`
C07	`05`	`X`	`ENG`	`@0 O-Glycosidases @2 FE @6 «O»-Glycosidases`
C07	`05`	`X`	`SPA`	`@0 O-Glycosidases @2 FE @6 «O»-Glycosidases`
C07	`06`	`X`	`FRE`	`@0 Glycosidases @2 FE`
C07	`06`	`X`	`ENG`	`@0 Glycosidases @2 FE`
C07	`06`	`X`	`SPA`	`@0 Glycosidases @2 FE`
C07	`07`	`X`	`FRE`	`@0 Hydrolases @2 FE`
C07	`07`	`X`	`ENG`	`@0 Hydrolases @2 FE`
C07	`07`	`X`	`SPA`	`@0 Hydrolases @2 FE`
C07	`08`	`X`	`FRE`	`@0 Enzyme`
C07	`08`	`X`	`ENG`	`@0 Enzyme`
C07	`08`	`X`	`SPA`	`@0 Enzima`
C07	`09`	`X`	`FRE`	`@0 Agrume @5 39`
C07	`09`	`X`	`ENG`	`@0 Citrus fruit @5 39`
C07	`09`	`X`	`SPA`	`@0 Agrios @5 39`
C07	`10`	`X`	`FRE`	`@0 Solvant @5 50`
C07	`10`	`X`	`ENG`	`@0 Solvent @5 50`
C07	`10`	`X`	`SPA`	`@0 Solvente @5 50`
N21				`@1 063`
N82				`@1 PSI`

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Le document en format XML

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<term>Analysis method</term>
<term>Aqueous solution</term>
<term>By product</term>
<term>Capillary electrophoresis</term>
<term>Citrus paradisi</term>
<term>Citrus sinensis</term>
<term>Ethanol</term>
<term>Extraction process</term>
<term>Fruit pulp</term>
<term>HPLC chromatography</term>
<term>Limonin</term>
<term>Methanol</term>
<term>Polygalacturonase</term>
<term>Purification</term>
<term>Skin</term>
<term>Upgrading</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Procédé extraction</term>
<term>Méthode analyse</term>
<term>Electrophorèse capillaire</term>
<term>Sous produit</term>
<term>Pulpe fruit</term>
<term>Peau</term>
<term>Albédo</term>
<term>Purification</term>
<term>Solution aqueuse</term>
<term>Citrus paradisi</term>
<term>Citrus sinensis</term>
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<front><div type="abstract" xml:lang="en">Limonin glucoside (LG) and phlorin were extracted from citrus fruit tissues and assayed by capillary electrophoresis (CE). LG was determined in dried [1.20 ± 0.10 mg of dry weight (dw)] and wet peel residues (1.16 ± 0.04 mg of dw), orange juice finisher pulp (0.58 ± 0.03 mg of dw), dried grapefruit seeds (2.70 ± 0.15 mg of dw), and 50 °Brix molasses (2225 ± 68 mg/L). Phlorin was purified from orange peel residue and grapefruit albedo, and concentrations were determined in some citrus products. Phlorin and LG were extracted from residues with water/pectinase or with water solutions of methanol and ethanol. Efficient LG extraction from grapefruit seeds (2.40 ± 0.15 mg/g) was achieved with 50-65% methanol, solvent polarity P'  7-8. Extracts were purified and concentrated by adsorptive resins and HPLC to obtain 95% pure compounds of LG and phlorin. CE analysis did not require extract purification beyond filtration. LG and phlorin migrated as anions in electropherograms containing peaks representing other citrus flavonoids and limonoid glucosides.</div>
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<fC01 i1="01" l="ENG"><s0>Limonin glucoside (LG) and phlorin were extracted from citrus fruit tissues and assayed by capillary electrophoresis (CE). LG was determined in dried [1.20 ± 0.10 mg of dry weight (dw)] and wet peel residues (1.16 ± 0.04 mg of dw), orange juice finisher pulp (0.58 ± 0.03 mg of dw), dried grapefruit seeds (2.70 ± 0.15 mg of dw), and 50 °Brix molasses (2225 ± 68 mg/L). Phlorin was purified from orange peel residue and grapefruit albedo, and concentrations were determined in some citrus products. Phlorin and LG were extracted from residues with water/pectinase or with water solutions of methanol and ethanol. Efficient LG extraction from grapefruit seeds (2.40 ± 0.15 mg/g) was achieved with 50-65% methanol, solvent polarity P'  7-8. Extracts were purified and concentrated by adsorptive resins and HPLC to obtain 95% pure compounds of LG and phlorin. CE analysis did not require extract purification beyond filtration. LG and phlorin migrated as anions in electropherograms containing peaks representing other citrus flavonoids and limonoid glucosides.</s0>
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<fC02 i1="01" i2="X"><s0>002A35F</s0>
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<fC03 i1="05" i2="X" l="FRE"><s0>Pulpe fruit</s0>
<s5>05</s5>
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<fC03 i1="05" i2="X" l="ENG"><s0>Fruit pulp</s0>
<s5>05</s5>
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<s5>06</s5>
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<s5>07</s5>
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<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Purification</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Purification</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Purificación</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Solution aqueuse</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Aqueous solution</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Solución acuosa</s0>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Citrus paradisi</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Citrus paradisi</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Citrus paradisi</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Limonine</s0>
<s5>15</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Limonin</s0>
<s5>15</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Limonina</s0>
<s5>15</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Méthanol</s0>
<s2>NK</s2>
<s2>FX</s2>
<s5>16</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Methanol</s0>
<s2>NK</s2>
<s2>FX</s2>
<s5>16</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Metanol</s0>
<s2>NK</s2>
<s2>FX</s2>
<s5>16</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Ethanol</s0>
<s2>NK</s2>
<s2>FR</s2>
<s2>FX</s2>
<s5>17</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Ethanol</s0>
<s2>NK</s2>
<s2>FR</s2>
<s2>FX</s2>
<s5>17</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Etanol</s0>
<s2>NK</s2>
<s2>FR</s2>
<s2>FX</s2>
<s5>17</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Polygalacturonase</s0>
<s2>NK</s2>
<s2>FE</s2>
<s5>33</s5>
</fC03>
<fC03 i1="15" i2="X" l="ENG"><s0>Polygalacturonase</s0>
<s2>NK</s2>
<s2>FE</s2>
<s5>33</s5>
</fC03>
<fC03 i1="15" i2="X" l="SPA"><s0>Polygalacturonase</s0>
<s2>NK</s2>
<s2>FE</s2>
<s5>33</s5>
</fC03>
<fC03 i1="16" i2="X" l="FRE"><s0>Chromatographie HPLC</s0>
<s5>34</s5>
</fC03>
<fC03 i1="16" i2="X" l="ENG"><s0>HPLC chromatography</s0>
<s5>34</s5>
</fC03>
<fC03 i1="16" i2="X" l="SPA"><s0>Cromatografía HPLC</s0>
<s5>34</s5>
</fC03>
<fC03 i1="17" i2="X" l="FRE"><s0>Valorisation</s0>
<s5>35</s5>
</fC03>
<fC03 i1="17" i2="X" l="ENG"><s0>Upgrading</s0>
<s5>35</s5>
</fC03>
<fC03 i1="17" i2="X" l="SPA"><s0>Valorización</s0>
<s5>35</s5>
</fC03>
<fC03 i1="18" i2="X" l="FRE"><s0>Phlorine</s0>
<s2>NK</s2>
<s4>INC</s4>
<s5>78</s5>
</fC03>
<fC03 i1="19" i2="X" l="FRE"><s0>Limonine glucoside</s0>
<s2>NK</s2>
<s4>INC</s4>
<s5>79</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>O-Glycosidases</s0>
<s2>FE</s2>
<s6>«O»-Glycosidases</s6>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>O-Glycosidases</s0>
<s2>FE</s2>
<s6>«O»-Glycosidases</s6>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>O-Glycosidases</s0>
<s2>FE</s2>
<s6>«O»-Glycosidases</s6>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Glycosidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Glycosidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Glycosidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Enzyme</s0>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Enzyme</s0>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Enzima</s0>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Agrume</s0>
<s5>39</s5>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>39</s5>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Agrios</s0>
<s5>39</s5>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Solvant</s0>
<s5>50</s5>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Solvent</s0>
<s5>50</s5>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Solvente</s0>
<s5>50</s5>
</fC07>
<fN21><s1>063</s1>
</fN21>
<fN82><s1>PSI</s1>
</fN82>
</pA>
</standard>
</inist>
</record>

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Extraction parameters and capillary electrophoresis analysis of limonin glucoside and phlorin in citrus byproducts

Extraction parameters and capillary electrophoresis analysis of limonin glucoside and phlorin in citrus byproducts

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