OrangerV1, PascalFrancis, Corpus, bibRecord, 000436

Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP

Identifieur interne : 000436 ( PascalFrancis/Corpus ); précédent : 000435; suivant : 000437

Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP

Auteurs : A. A. Omar ; J. W. Grosser

Source :

Acta horticulturae [ 0567-7572 ] ; 2007.

RBID : Pascal:07-0538144

Descripteurs français

Pascal (Inist)
- Plante transgénique, Cultivar, Bactériose, Résistance microorganisme, Gène indicateur, Gène structure, Multigène, Protoplaste, Régénération, Transformation génétique, Citrus sinensis, Oryza sativa, Xanthomonas axonopodis, DNA complémentaire, Plasmide, Protéine fluorescente verte, Culture cellulaire, Système combiné, cv Hamlin, Gène gfp, Gène Xa21, Xanthomonas oryzae, Chancre plante.

English descriptors

KwdEn :
- Bacteriosis, Cell culture, Citrus sinensis, Combined system, Complementary DNA, Cultivar, Genetic transformation, Green fluorescent protein, Microorganism resistance, Multigene family, Plant cankers, Protoplast, Regeneration, Reporter gene, Structural gene, Transgenic plant, Xanthomonas axonopodis.

Abstract

[...] Plasmid DNA (pARS108) encoding the non-destructive selectable marker EGFP (Enhanced Green Fluorescent Protein) gene, and the plasmid cDNA of the Xa21 gene (pXa21-mtaq) were co-transformed into 'Hamlin' orange protoplasts using polyethylene glycol. More than 150 transgenic embryoids were recovered. Over a thousand transgenic plantlets GFP positive were regenerated from 150 independent transformation events. PCR analysis revealed the presence of the cDNA of the Xa21 and the GFP genes in some of the transgenic plantlets. The recovery of transgenic plants was expedited by in vitro grafting.[...]

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A08	`01`	`1`	`ENG`	`@1 Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP`
A09	`01`	`1`	`ENG`	`@1 Proceedings of the International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species : Daytona Beach, Florida, USA, October 10-14, 2005`
A11	`01`	`1`		`@1 OMAR (A. A.)`
A11	`02`	`1`		`@1 GROSSER (J. W.)`
A12	`01`	`1`		`@1 LITZ (Richard E.) @9 ed.`
A12	`02`	`1`		`@1 SCORZA (Ralph) @9 ed.`
A14	`01`			`@1 University of Florida Citrus Research and Education Center @2 Lake Alfred, FL @3 USA @Z 1 aut. @Z 2 aut.`
A18	`01`	`1`		`@1 International Society for Horticultural Science. Section on Tropical and Subtropical Fruits @3 INT @9 org-cong.`
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A21				`@1 2007`
A23	`01`			`@0 ENG`
A26	`01`			`@0 978-90-6605-219-2`
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C01	`01`		`ENG`	@0 [...] Plasmid DNA (pARS108) encoding the non-destructive selectable marker EGFP (Enhanced Green Fluorescent Protein) gene, and the plasmid cDNA of the Xa21 gene (pXa21-mtaq) were co-transformed into 'Hamlin' orange protoplasts using polyethylene glycol. More than 150 transgenic embryoids were recovered. Over a thousand transgenic plantlets GFP positive were regenerated from 150 independent transformation events. PCR analysis revealed the presence of the cDNA of the Xa21 and the GFP genes in some of the transgenic plantlets. The recovery of transgenic plants was expedited by in vitro grafting.[...]
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C03	`04`	`X`	`FRE`	`@0 Résistance microorganisme @5 04`
C03	`04`	`X`	`ENG`	`@0 Microorganism resistance @5 04`
C03	`04`	`X`	`SPA`	`@0 Resistencia microorganismo @5 04`
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C03	`06`	`X`	`SPA`	`@0 Gen estructura @5 06`
C03	`07`	`X`	`FRE`	`@0 Multigène @5 07`
C03	`07`	`X`	`ENG`	`@0 Multigene family @5 07`
C03	`07`	`X`	`SPA`	`@0 Multigén @5 07`
C03	`08`	`X`	`FRE`	`@0 Protoplaste @5 08`
C03	`08`	`X`	`ENG`	`@0 Protoplast @5 08`
C03	`08`	`X`	`SPA`	`@0 Protoplasto @5 08`
C03	`09`	`X`	`FRE`	`@0 Régénération @5 09`
C03	`09`	`X`	`ENG`	`@0 Regeneration @5 09`
C03	`09`	`X`	`SPA`	`@0 Regeneración @5 09`
C03	`10`	`X`	`FRE`	`@0 Transformation génétique @5 10`
C03	`10`	`X`	`ENG`	`@0 Genetic transformation @5 10`
C03	`10`	`X`	`SPA`	`@0 Transformación genética @5 10`
C03	`11`	`X`	`FRE`	`@0 Citrus sinensis @2 NS @5 12`
C03	`11`	`X`	`ENG`	`@0 Citrus sinensis @2 NS @5 12`
C03	`11`	`X`	`SPA`	`@0 Citrus sinensis @2 NS @5 12`
C03	`12`	`X`	`FRE`	`@0 Oryza sativa @4 INC @5 13`
C03	`13`	`X`	`FRE`	`@0 Xanthomonas axonopodis @2 NS @5 14`
C03	`13`	`X`	`ENG`	`@0 Xanthomonas axonopodis @2 NS @5 14`
C03	`13`	`X`	`SPA`	`@0 Xanthomonas axonopodis @2 NS @5 14`
C03	`14`	`X`	`FRE`	`@0 DNA complémentaire @5 15`
C03	`14`	`X`	`ENG`	`@0 Complementary DNA @5 15`
C03	`14`	`X`	`SPA`	`@0 DNA complementario @5 15`
C03	`15`	`X`	`FRE`	`@0 Plasmide @4 INC @5 16`
C03	`16`	`X`	`FRE`	`@0 Protéine fluorescente verte @5 17`
C03	`16`	`X`	`ENG`	`@0 Green fluorescent protein @5 17`
C03	`16`	`X`	`SPA`	`@0 Proteína fluorescente verde @5 17`
C03	`17`	`X`	`FRE`	`@0 Culture cellulaire @5 20`
C03	`17`	`X`	`ENG`	`@0 Cell culture @5 20`
C03	`17`	`X`	`SPA`	`@0 Cultivo celular @5 20`
C03	`18`	`X`	`FRE`	`@0 Système combiné @5 21`
C03	`18`	`X`	`ENG`	`@0 Combined system @5 21`
C03	`18`	`X`	`SPA`	`@0 Sistema combinado @5 21`
C03	`19`	`X`	`FRE`	`@0 cv Hamlin @4 INC @5 68`
C03	`20`	`X`	`FRE`	`@0 Gène gfp @4 INC @5 69`
C03	`21`	`X`	`FRE`	`@0 Gène Xa21 @4 INC @5 70`
C03	`22`	`X`	`FRE`	`@0 Xanthomonas oryzae @4 INC @5 71`
C03	`23`	`X`	`FRE`	`@0 Chancre plante @4 CD @5 96`
C03	`23`	`X`	`ENG`	`@0 Plant cankers @4 CD @5 96`
C03	`23`	`X`	`SPA`	`@0 Cancro @4 CD @5 96`
C07	`01`	`X`	`FRE`	`@0 Infection`
C07	`01`	`X`	`ENG`	`@0 Infection`
C07	`01`	`X`	`SPA`	`@0 Infección`
C07	`02`	`X`	`FRE`	`@0 Rutaceae @2 NS`
C07	`02`	`X`	`ENG`	`@0 Rutaceae @2 NS`
C07	`02`	`X`	`SPA`	`@0 Rutaceae @2 NS`
C07	`03`	`X`	`FRE`	`@0 Dicotyledones @2 NS`
C07	`03`	`X`	`ENG`	`@0 Dicotyledones @2 NS`
C07	`03`	`X`	`SPA`	`@0 Dicotyledones @2 NS`
C07	`04`	`X`	`FRE`	`@0 Angiospermae @2 NS`
C07	`04`	`X`	`ENG`	`@0 Angiospermae @2 NS`
C07	`04`	`X`	`SPA`	`@0 Angiospermae @2 NS`
C07	`05`	`X`	`FRE`	`@0 Spermatophyta @2 NS`
C07	`05`	`X`	`ENG`	`@0 Spermatophyta @2 NS`
C07	`05`	`X`	`SPA`	`@0 Spermatophyta @2 NS`
C07	`06`	`X`	`FRE`	`@0 Pseudomonadaceae @2 NS`
C07	`06`	`X`	`ENG`	`@0 Pseudomonadaceae @2 NS`
C07	`06`	`X`	`SPA`	`@0 Pseudomonadaceae @2 NS`
C07	`07`	`X`	`FRE`	`@0 Pseudomonadales @2 NS`
C07	`07`	`X`	`ENG`	`@0 Pseudomonadales @2 NS`
C07	`07`	`X`	`SPA`	`@0 Pseudomonadales @2 NS`
C07	`08`	`X`	`FRE`	`@0 Bactérie`
C07	`08`	`X`	`ENG`	`@0 Bacteria`
C07	`08`	`X`	`SPA`	`@0 Bacteria`
C07	`09`	`X`	`FRE`	`@0 Agrume @4 INC @5 31`
C07	`10`	`X`	`FRE`	`@0 Arbre fruitier @4 INC @5 32`
C07	`11`	`X`	`FRE`	`@0 Organisme génétiquement modifié @4 INC @5 33`
C07	`12`	`X`	`FRE`	`@0 Plante fruitière @5 34`
C07	`12`	`X`	`ENG`	`@0 Fruit crop @5 34`
C07	`12`	`X`	`SPA`	`@0 Planta frutal @5 34`
C07	`13`	`X`	`FRE`	`@0 Phytopathogène @5 35`
C07	`13`	`X`	`ENG`	`@0 Plant pathogen @5 35`
C07	`13`	`X`	`SPA`	`@0 Fitopatógeno @5 35`
C07	`14`	`X`	`FRE`	`@0 Sensibilité résistance @4 INC @5 36`
C07	`15`	`X`	`FRE`	`@0 Amélioration génétique @5 39`
C07	`15`	`X`	`ENG`	`@0 Genetic improvement @5 39`
C07	`15`	`X`	`SPA`	`@0 Enriquecimiento genético @5 39`
C07	`16`	`X`	`FRE`	`@0 Biotechnologie @5 40`
C07	`16`	`X`	`ENG`	`@0 Biotechnology @5 40`
C07	`16`	`X`	`SPA`	`@0 Biotecnología @5 40`
C07	`17`	`X`	`FRE`	`@0 Génie génétique @5 41`
C07	`17`	`X`	`ENG`	`@0 Genetic engineering @5 41`
C07	`17`	`X`	`SPA`	`@0 Ingeniería genética @5 41`
C07	`18`	`X`	`FRE`	`@0 Plante ligneuse @4 INC @5 42`
C07	`19`	`X`	`FRE`	`@0 Acide nucléique @4 INC @5 43`
C07	`20`	`X`	`FRE`	`@0 Composé biochimique @4 INC @5 44`
C07	`21`	`X`	`FRE`	`@0 Gène @4 INC @5 45`
C07	`22`	`X`	`FRE`	`@0 Arboriculture @5 64`
C07	`22`	`X`	`ENG`	`@0 Arboriculture @5 64`
C07	`22`	`X`	`SPA`	`@0 Arboricultura @5 64`
C07	`23`	`X`	`FRE`	`@0 Horticulture @5 65`
C07	`23`	`X`	`ENG`	`@0 Horticulture @5 65`
C07	`23`	`X`	`SPA`	`@0 Horticultura @5 65`
C07	`24`	`X`	`FRE`	`@0 Industrie fruits @5 66`
C07	`24`	`X`	`ENG`	`@0 Fruit industry @5 66`
C07	`24`	`X`	`SPA`	`@0 Industria frutas @5 66`
N21				`@1 351`

A30	`01`	`1`	`ENG`	`@1 International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species @3 Daytona Beach FL USA @4 2005`

Format Inist (serveur)

NO :	PASCAL 07-0538144 INIST
ET :	Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP
AU :	OMAR (A. A.); GROSSER (J. W.); LITZ (Richard E.); SCORZA (Ralph)
AF :	University of Florida Citrus Research and Education Center/Lake Alfred, FL/Etats-Unis (1 aut., 2 aut.)
DT :	Publication en série; Congrès; Niveau analytique
SO :	Acta horticulturae; ISSN 0567-7572; Pays-Bas; Da. 2007; No. 738; Pp. 235-243; Bibl. 1 p.1/2
LA :	Anglais
EA :	[...] Plasmid DNA (pARS108) encoding the non-destructive selectable marker EGFP (Enhanced Green Fluorescent Protein) gene, and the plasmid cDNA of the Xa21 gene (pXa21-mtaq) were co-transformed into 'Hamlin' orange protoplasts using polyethylene glycol. More than 150 transgenic embryoids were recovered. Over a thousand transgenic plantlets GFP positive were regenerated from 150 independent transformation events. PCR analysis revealed the presence of the cDNA of the Xa21 and the GFP genes in some of the transgenic plantlets. The recovery of transgenic plants was expedited by in vitro grafting.[...]
CC :	002A31C02A5B; 002A31C07C3; 002A32D02B; 002A32D03B2; 215
FD :	Plante transgénique; Cultivar; Bactériose; Résistance microorganisme; Gène indicateur; Gène structure; Multigène; Protoplaste; Régénération; Transformation génétique; Citrus sinensis; Oryza sativa; Xanthomonas axonopodis; DNA complémentaire; Plasmide; Protéine fluorescente verte; Culture cellulaire; Système combiné; cv Hamlin; Gène gfp; Gène Xa21; Xanthomonas oryzae; Chancre plante
FG :	Infection; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Pseudomonadaceae; Pseudomonadales; Bactérie; Agrume; Arbre fruitier; Organisme génétiquement modifié; Plante fruitière; Phytopathogène; Sensibilité résistance; Amélioration génétique; Biotechnologie; Génie génétique; Plante ligneuse; Acide nucléique; Composé biochimique; Gène; Arboriculture; Horticulture; Industrie fruits
ED :	Transgenic plant; Cultivar; Bacteriosis; Microorganism resistance; Reporter gene; Structural gene; Multigene family; Protoplast; Regeneration; Genetic transformation; Citrus sinensis; Xanthomonas axonopodis; Complementary DNA; Green fluorescent protein; Cell culture; Combined system; Plant cankers
EG :	Infection; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Pseudomonadaceae; Pseudomonadales; Bacteria; Fruit crop; Plant pathogen; Genetic improvement; Biotechnology; Genetic engineering; Arboriculture; Horticulture; Fruit industry
SD :	Planta transgénica; Cultivar; Bacteriosis; Resistencia microorganismo; Gen marcador; Gen estructura; Multigén; Protoplasto; Regeneración; Transformación genética; Citrus sinensis; Xanthomonas axonopodis; DNA complementario; Proteína fluorescente verde; Cultivo celular; Sistema combinado; Cancro
LO :	INIST-15963.354000153599050230
ID :	07-0538144

Links to Exploration step

Pascal:07-0538144

Le document en format XML

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<front><div type="abstract" xml:lang="en">[...] Plasmid DNA (pARS108) encoding the non-destructive selectable marker EGFP (Enhanced Green Fluorescent Protein) gene, and the plasmid cDNA of the Xa21 gene (pXa21-mtaq) were co-transformed into 'Hamlin' orange protoplasts using polyethylene glycol. More than 150 transgenic embryoids were recovered. Over a thousand transgenic plantlets GFP positive were regenerated from 150 independent transformation events. PCR analysis revealed the presence of the cDNA of the Xa21 and the GFP genes in some of the transgenic plantlets. The recovery of transgenic plants was expedited by in vitro grafting.[...]</div>
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</fA43>
<fA44><s0>0000</s0>
<s1>© 2007 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>1 p.1/2</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>07-0538144</s0>
</fA47>
<fA60><s1>P</s1>
<s2>C</s2>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Acta horticulturae</s0>
</fA64>
<fA66 i1="01"><s0>NLD</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>[...] Plasmid DNA (pARS108) encoding the non-destructive selectable marker EGFP (Enhanced Green Fluorescent Protein) gene, and the plasmid cDNA of the Xa21 gene (pXa21-mtaq) were co-transformed into 'Hamlin' orange protoplasts using polyethylene glycol. More than 150 transgenic embryoids were recovered. Over a thousand transgenic plantlets GFP positive were regenerated from 150 independent transformation events. PCR analysis revealed the presence of the cDNA of the Xa21 and the GFP genes in some of the transgenic plantlets. The recovery of transgenic plants was expedited by in vitro grafting.[...]</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A31C02A5B</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002A31C07C3</s0>
</fC02>
<fC02 i1="03" i2="X"><s0>002A32D02B</s0>
</fC02>
<fC02 i1="04" i2="X"><s0>002A32D03B2</s0>
</fC02>
<fC02 i1="05" i2="X"><s0>215</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Plante transgénique</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Transgenic plant</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Planta transgénica</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Cultivar</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Cultivar</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Cultivar</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Bactériose</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Bacteriosis</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Bacteriosis</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Résistance microorganisme</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Microorganism resistance</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Resistencia microorganismo</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Gène indicateur</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Reporter gene</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Gen marcador</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Gène structure</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Structural gene</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Gen estructura</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Multigène</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Multigene family</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Multigén</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Protoplaste</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Protoplast</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Protoplasto</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Régénération</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Regeneration</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Regeneración</s0>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Transformation génétique</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Genetic transformation</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Transformación genética</s0>
<s5>10</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>12</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>12</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>12</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Oryza sativa</s0>
<s4>INC</s4>
<s5>13</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Xanthomonas axonopodis</s0>
<s2>NS</s2>
<s5>14</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Xanthomonas axonopodis</s0>
<s2>NS</s2>
<s5>14</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Xanthomonas axonopodis</s0>
<s2>NS</s2>
<s5>14</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>DNA complémentaire</s0>
<s5>15</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Complementary DNA</s0>
<s5>15</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>DNA complementario</s0>
<s5>15</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Plasmide</s0>
<s4>INC</s4>
<s5>16</s5>
</fC03>
<fC03 i1="16" i2="X" l="FRE"><s0>Protéine fluorescente verte</s0>
<s5>17</s5>
</fC03>
<fC03 i1="16" i2="X" l="ENG"><s0>Green fluorescent protein</s0>
<s5>17</s5>
</fC03>
<fC03 i1="16" i2="X" l="SPA"><s0>Proteína fluorescente verde</s0>
<s5>17</s5>
</fC03>
<fC03 i1="17" i2="X" l="FRE"><s0>Culture cellulaire</s0>
<s5>20</s5>
</fC03>
<fC03 i1="17" i2="X" l="ENG"><s0>Cell culture</s0>
<s5>20</s5>
</fC03>
<fC03 i1="17" i2="X" l="SPA"><s0>Cultivo celular</s0>
<s5>20</s5>
</fC03>
<fC03 i1="18" i2="X" l="FRE"><s0>Système combiné</s0>
<s5>21</s5>
</fC03>
<fC03 i1="18" i2="X" l="ENG"><s0>Combined system</s0>
<s5>21</s5>
</fC03>
<fC03 i1="18" i2="X" l="SPA"><s0>Sistema combinado</s0>
<s5>21</s5>
</fC03>
<fC03 i1="19" i2="X" l="FRE"><s0>cv Hamlin</s0>
<s4>INC</s4>
<s5>68</s5>
</fC03>
<fC03 i1="20" i2="X" l="FRE"><s0>Gène gfp</s0>
<s4>INC</s4>
<s5>69</s5>
</fC03>
<fC03 i1="21" i2="X" l="FRE"><s0>Gène Xa21</s0>
<s4>INC</s4>
<s5>70</s5>
</fC03>
<fC03 i1="22" i2="X" l="FRE"><s0>Xanthomonas oryzae</s0>
<s4>INC</s4>
<s5>71</s5>
</fC03>
<fC03 i1="23" i2="X" l="FRE"><s0>Chancre plante</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="23" i2="X" l="ENG"><s0>Plant cankers</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="23" i2="X" l="SPA"><s0>Cancro</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Infection</s0>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Infection</s0>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Infección</s0>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Pseudomonadaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Pseudomonadaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Pseudomonadaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>Pseudomonadales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>Pseudomonadales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>Pseudomonadales</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Bactérie</s0>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Bacteria</s0>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Bacteria</s0>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Agrume</s0>
<s4>INC</s4>
<s5>31</s5>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Arbre fruitier</s0>
<s4>INC</s4>
<s5>32</s5>
</fC07>
<fC07 i1="11" i2="X" l="FRE"><s0>Organisme génétiquement modifié</s0>
<s4>INC</s4>
<s5>33</s5>
</fC07>
<fC07 i1="12" i2="X" l="FRE"><s0>Plante fruitière</s0>
<s5>34</s5>
</fC07>
<fC07 i1="12" i2="X" l="ENG"><s0>Fruit crop</s0>
<s5>34</s5>
</fC07>
<fC07 i1="12" i2="X" l="SPA"><s0>Planta frutal</s0>
<s5>34</s5>
</fC07>
<fC07 i1="13" i2="X" l="FRE"><s0>Phytopathogène</s0>
<s5>35</s5>
</fC07>
<fC07 i1="13" i2="X" l="ENG"><s0>Plant pathogen</s0>
<s5>35</s5>
</fC07>
<fC07 i1="13" i2="X" l="SPA"><s0>Fitopatógeno</s0>
<s5>35</s5>
</fC07>
<fC07 i1="14" i2="X" l="FRE"><s0>Sensibilité résistance</s0>
<s4>INC</s4>
<s5>36</s5>
</fC07>
<fC07 i1="15" i2="X" l="FRE"><s0>Amélioration génétique</s0>
<s5>39</s5>
</fC07>
<fC07 i1="15" i2="X" l="ENG"><s0>Genetic improvement</s0>
<s5>39</s5>
</fC07>
<fC07 i1="15" i2="X" l="SPA"><s0>Enriquecimiento genético</s0>
<s5>39</s5>
</fC07>
<fC07 i1="16" i2="X" l="FRE"><s0>Biotechnologie</s0>
<s5>40</s5>
</fC07>
<fC07 i1="16" i2="X" l="ENG"><s0>Biotechnology</s0>
<s5>40</s5>
</fC07>
<fC07 i1="16" i2="X" l="SPA"><s0>Biotecnología</s0>
<s5>40</s5>
</fC07>
<fC07 i1="17" i2="X" l="FRE"><s0>Génie génétique</s0>
<s5>41</s5>
</fC07>
<fC07 i1="17" i2="X" l="ENG"><s0>Genetic engineering</s0>
<s5>41</s5>
</fC07>
<fC07 i1="17" i2="X" l="SPA"><s0>Ingeniería genética</s0>
<s5>41</s5>
</fC07>
<fC07 i1="18" i2="X" l="FRE"><s0>Plante ligneuse</s0>
<s4>INC</s4>
<s5>42</s5>
</fC07>
<fC07 i1="19" i2="X" l="FRE"><s0>Acide nucléique</s0>
<s4>INC</s4>
<s5>43</s5>
</fC07>
<fC07 i1="20" i2="X" l="FRE"><s0>Composé biochimique</s0>
<s4>INC</s4>
<s5>44</s5>
</fC07>
<fC07 i1="21" i2="X" l="FRE"><s0>Gène</s0>
<s4>INC</s4>
<s5>45</s5>
</fC07>
<fC07 i1="22" i2="X" l="FRE"><s0>Arboriculture</s0>
<s5>64</s5>
</fC07>
<fC07 i1="22" i2="X" l="ENG"><s0>Arboriculture</s0>
<s5>64</s5>
</fC07>
<fC07 i1="22" i2="X" l="SPA"><s0>Arboricultura</s0>
<s5>64</s5>
</fC07>
<fC07 i1="23" i2="X" l="FRE"><s0>Horticulture</s0>
<s5>65</s5>
</fC07>
<fC07 i1="23" i2="X" l="ENG"><s0>Horticulture</s0>
<s5>65</s5>
</fC07>
<fC07 i1="23" i2="X" l="SPA"><s0>Horticultura</s0>
<s5>65</s5>
</fC07>
<fC07 i1="24" i2="X" l="FRE"><s0>Industrie fruits</s0>
<s5>66</s5>
</fC07>
<fC07 i1="24" i2="X" l="ENG"><s0>Fruit industry</s0>
<s5>66</s5>
</fC07>
<fC07 i1="24" i2="X" l="SPA"><s0>Industria frutas</s0>
<s5>66</s5>
</fC07>
<fN21><s1>351</s1>
</fN21>
</pA>
<pR><fA30 i1="01" i2="1" l="ENG"><s1>International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species</s1>
<s3>Daytona Beach FL USA</s3>
<s4>2005</s4>
</fA30>
</pR>
</standard>
<server><NO>PASCAL 07-0538144 INIST</NO>
<ET>Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP</ET>
<AU>OMAR (A. A.); GROSSER (J. W.); LITZ (Richard E.); SCORZA (Ralph)</AU>
<AF>University of Florida Citrus Research and Education Center/Lake Alfred, FL/Etats-Unis (1 aut., 2 aut.)</AF>
<DT>Publication en série; Congrès; Niveau analytique</DT>
<SO>Acta horticulturae; ISSN 0567-7572; Pays-Bas; Da. 2007; No. 738; Pp. 235-243; Bibl. 1 p.1/2</SO>
<LA>Anglais</LA>
<EA>[...] Plasmid DNA (pARS108) encoding the non-destructive selectable marker EGFP (Enhanced Green Fluorescent Protein) gene, and the plasmid cDNA of the Xa21 gene (pXa21-mtaq) were co-transformed into 'Hamlin' orange protoplasts using polyethylene glycol. More than 150 transgenic embryoids were recovered. Over a thousand transgenic plantlets GFP positive were regenerated from 150 independent transformation events. PCR analysis revealed the presence of the cDNA of the Xa21 and the GFP genes in some of the transgenic plantlets. The recovery of transgenic plants was expedited by in vitro grafting.[...]</EA>
<CC>002A31C02A5B; 002A31C07C3; 002A32D02B; 002A32D03B2; 215</CC>
<FD>Plante transgénique; Cultivar; Bactériose; Résistance microorganisme; Gène indicateur; Gène structure; Multigène; Protoplaste; Régénération; Transformation génétique; Citrus sinensis; Oryza sativa; Xanthomonas axonopodis; DNA complémentaire; Plasmide; Protéine fluorescente verte; Culture cellulaire; Système combiné; cv Hamlin; Gène gfp; Gène Xa21; Xanthomonas oryzae; Chancre plante</FD>
<FG>Infection; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Pseudomonadaceae; Pseudomonadales; Bactérie; Agrume; Arbre fruitier; Organisme génétiquement modifié; Plante fruitière; Phytopathogène; Sensibilité résistance; Amélioration génétique; Biotechnologie; Génie génétique; Plante ligneuse; Acide nucléique; Composé biochimique; Gène; Arboriculture; Horticulture; Industrie fruits</FG>
<ED>Transgenic plant; Cultivar; Bacteriosis; Microorganism resistance; Reporter gene; Structural gene; Multigene family; Protoplast; Regeneration; Genetic transformation; Citrus sinensis; Xanthomonas axonopodis; Complementary DNA; Green fluorescent protein; Cell culture; Combined system; Plant cankers</ED>
<EG>Infection; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Pseudomonadaceae; Pseudomonadales; Bacteria; Fruit crop; Plant pathogen; Genetic improvement; Biotechnology; Genetic engineering; Arboriculture; Horticulture; Fruit industry</EG>
<SD>Planta transgénica; Cultivar; Bacteriosis; Resistencia microorganismo; Gen marcador; Gen estructura; Multigén; Protoplasto; Regeneración; Transformación genética; Citrus sinensis; Xanthomonas axonopodis; DNA complementario; Proteína fluorescente verde; Cultivo celular; Sistema combinado; Cancro</SD>
<LO>INIST-15963.354000153599050230</LO>
<ID>07-0538144</ID>
</server>
</inist>
</record>

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Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP

Protoplast co-transformation and regeneration of transgenic 'Hamlin' sweet orange plants containing a cDNA Xa21 xanthomonas resistance gene and GFP

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