OrangerV1, PascalFrancis, Corpus, bibRecord, 000437

Agrobacterium-mediated transformation of citrus using two binary vectors

Identifieur interne : 000437 ( PascalFrancis/Corpus ); précédent : 000436; suivant : 000438

Agrobacterium-mediated transformation of citrus using two binary vectors

Auteurs : Jaya R. Soneji ; CHUNXIAN CHEN ; M. Nageswara Rao ; SHU HUANG ; Young A. Choi ; Fred G. Jr Gmitter

Source :

Acta horticulturae [ 0567-7572 ] ; 2007.

RBID : Pascal:07-0530119

Descripteurs français

Pascal (Inist)
- Agrume, Plante transgénique, Transfection, Transfert génétique, Transformation génétique, Vecteur, Système binaire, Agrobacterium, Citrus sinensis, Cultivar, Plasmide Ti, β-Glucuronidase, Protéine fluorescente verte, Gène hybride, Gène indicateur, Gène structure, Gène gfp, Gène gus, cv Midsweet, Poncirus.

English descriptors

KwdEn :
- Agrobacterium, Binary system, Citrus fruit, Citrus sinensis, Cultivar, Genetic transfer, Genetic transformation, Green fluorescent protein, Hybrid gene, Reporter gene, Structural gene, Ti Plasmid, Transfection, Transgenic plant, Vector, β-Glucuronidase.

Abstract

In the present study, epicotyl explants of 'Midsweet' sweet orange (Citrus sinensis L. Osb.) were transformed using a mixture of two binary vectors individually introduced into Agrobacterium strain AGL1. One binary Ti vector was assembled from pCAMBIA2301 with GUS as the reporter gene while the other binary Ti vector was assembled from pGreen0029 with GFP as the reporter gene. Two gene constructs containing specific gene fragments from Poncirus were used. The epicotyl explants were transformed by use of 1:1 mixture of AGL1 carrying the binary vectors separately. Shoots regenerated from transformed epicotyl explants on selective medium were micrografted onto 'Carrizo' seedlings. The GFP expression and the GUS activity in the leaves were also determined. Further confirmation by molecular analyses is currently underway.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A08	`01`	`1`	`ENG`	`@1 Agrobacterium-mediated transformation of citrus using two binary vectors`
A09	`01`	`1`	`ENG`	`@1 Proceedings of the International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species : Daytona Beach, Florida, USA, October 10-14, 2005`
A11	`01`	`1`		`@1 SONEJI (Jaya R.)`
A11	`02`	`1`		`@1 CHUNXIAN CHEN`
A11	`03`	`1`		`@1 NAGESWARA RAO (M.)`
A11	`04`	`1`		`@1 SHU HUANG`
A11	`05`	`1`		`@1 CHOI (Young A.)`
A11	`06`	`1`		`@1 GMITTER (Fred G. JR)`
A12	`01`	`1`		`@1 LITZ (Richard E.) @9 ed.`
A12	`02`	`1`		`@1 SCORZA (Ralph) @9 ed.`
A14	`01`			`@1 University of Florida Citrus Research and Education Center 700 Experiment Station Road @2 Lake Alfred, FL 33850 @3 USA @Z 1 aut. @Z 2 aut. @Z 3 aut. @Z 4 aut. @Z 5 aut. @Z 6 aut.`
A18	`01`	`1`		`@1 International Society for Horticultural Science. Section on Tropical and Subtropical Fruits @3 INT @9 org-cong.`
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C03	`08`	`X`	`FRE`	`@0 Agrobacterium @2 NS @5 10`
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C03	`08`	`X`	`SPA`	`@0 Agrobacterium @2 NS @5 10`
C03	`09`	`X`	`FRE`	`@0 Citrus sinensis @2 NS @5 11`
C03	`09`	`X`	`ENG`	`@0 Citrus sinensis @2 NS @5 11`
C03	`09`	`X`	`SPA`	`@0 Citrus sinensis @2 NS @5 11`
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C07	`02`	`X`	`FRE`	`@0 Bactérie`
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A30	`01`	`1`	`ENG`	`@1 International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species @3 Daytona Beach FL USA @4 2005`

Format Inist (serveur)

NO :	PASCAL 07-0530119 INIST
ET :	Agrobacterium-mediated transformation of citrus using two binary vectors
AU :	SONEJI (Jaya R.); CHUNXIAN CHEN; NAGESWARA RAO (M.); SHU HUANG; CHOI (Young A.); GMITTER (Fred G. JR); LITZ (Richard E.); SCORZA (Ralph)
AF :	University of Florida Citrus Research and Education Center 700 Experiment Station Road/Lake Alfred, FL 33850/Etats-Unis (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut.)
DT :	Publication en série; Congrès; Niveau analytique
SO :	Acta horticulturae; ISSN 0567-7572; Pays-Bas; Da. 2007; No. 738; Pp. 261-264; Bibl. 1/4 p.
LA :	Anglais
EA :	In the present study, epicotyl explants of 'Midsweet' sweet orange (Citrus sinensis L. Osb.) were transformed using a mixture of two binary vectors individually introduced into Agrobacterium strain AGL1. One binary Ti vector was assembled from pCAMBIA2301 with GUS as the reporter gene while the other binary Ti vector was assembled from pGreen0029 with GFP as the reporter gene. Two gene constructs containing specific gene fragments from Poncirus were used. The epicotyl explants were transformed by use of 1:1 mixture of AGL1 carrying the binary vectors separately. Shoots regenerated from transformed epicotyl explants on selective medium were micrografted onto 'Carrizo' seedlings. The GFP expression and the GUS activity in the leaves were also determined. Further confirmation by molecular analyses is currently underway.
CC :	002A31C02A5B; 002A32D02B; 215
FD :	Agrume; Plante transgénique; Transfection; Transfert génétique; Transformation génétique; Vecteur; Système binaire; Agrobacterium; Citrus sinensis; Cultivar; Plasmide Ti; β-Glucuronidase; Protéine fluorescente verte; Gène hybride; Gène indicateur; Gène structure; Gène gfp; Gène gus; cv Midsweet; Poncirus
FG :	Rhizobiaceae; Bactérie; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; O-Glycosidases; Glycosidases; Hydrolases; Enzyme; Arbre fruitier; Plante fruitière; Organisme génétiquement modifié; Plante ligneuse; Composé biochimique; Biotechnologie; Génie génétique; Arboriculture; Horticulture; Industrie fruits; Bactérie Gram négatif; Procaryote
ED :	Citrus fruit; Transgenic plant; Transfection; Genetic transfer; Genetic transformation; Vector; Binary system; Agrobacterium; Citrus sinensis; Cultivar; Ti Plasmid; β-Glucuronidase; Green fluorescent protein; Hybrid gene; Reporter gene; Structural gene
EG :	Rhizobiaceae; Bacteria; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; O-Glycosidases; Glycosidases; Hydrolases; Enzyme; Fruit tree; Fruit crop; Genetically modified organism; Woody plant; Biochemical compound; Biotechnology; Genetic engineering; Arboriculture; Horticulture; Fruit industry
SD :	Agrios; Planta transgénica; Transfección; Transferencia genética; Transformación genética; Vector; Sistema binario; Agrobacterium; Citrus sinensis; Cultivar; Plasmido Ti; β-Glucuronidase; Proteína fluorescente verde; Gen híbrido; Gen marcador; Gen estructura
LO :	INIST-15963.354000153599050260
ID :	07-0530119

Links to Exploration step

Pascal:07-0530119

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Agrobacterium-mediated transformation of citrus using two binary vectors</title>
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<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Choi, Young A" sort="Choi, Young A" uniqKey="Choi Y" first="Young A." last="Choi">Young A. Choi</name>
<affiliation><inist:fA14 i1="01"><s1>University of Florida Citrus Research and Education Center 700 Experiment Station Road</s1>
<s2>Lake Alfred, FL 33850</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
<author><name sortKey="Gmitter, Fred G Jr" sort="Gmitter, Fred G Jr" uniqKey="Gmitter F" first="Fred G. Jr" last="Gmitter">Fred G. Jr Gmitter</name>
<affiliation><inist:fA14 i1="01"><s1>University of Florida Citrus Research and Education Center 700 Experiment Station Road</s1>
<s2>Lake Alfred, FL 33850</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Acta horticulturae</title>
<idno type="ISSN">0567-7572</idno>
<imprint><date when="2007">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Acta horticulturae</title>
<idno type="ISSN">0567-7572</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium</term>
<term>Binary system</term>
<term>Citrus fruit</term>
<term>Citrus sinensis</term>
<term>Cultivar</term>
<term>Genetic transfer</term>
<term>Genetic transformation</term>
<term>Green fluorescent protein</term>
<term>Hybrid gene</term>
<term>Reporter gene</term>
<term>Structural gene</term>
<term>Ti Plasmid</term>
<term>Transfection</term>
<term>Transgenic plant</term>
<term>Vector</term>
<term>β-Glucuronidase</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Agrume</term>
<term>Plante transgénique</term>
<term>Transfection</term>
<term>Transfert génétique</term>
<term>Transformation génétique</term>
<term>Vecteur</term>
<term>Système binaire</term>
<term>Agrobacterium</term>
<term>Citrus sinensis</term>
<term>Cultivar</term>
<term>Plasmide Ti</term>
<term>β-Glucuronidase</term>
<term>Protéine fluorescente verte</term>
<term>Gène hybride</term>
<term>Gène indicateur</term>
<term>Gène structure</term>
<term>Gène gfp</term>
<term>Gène gus</term>
<term>cv Midsweet</term>
<term>Poncirus</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">In the present study, epicotyl explants of 'Midsweet' sweet orange (Citrus sinensis L. Osb.) were transformed using a mixture of two binary vectors individually introduced into Agrobacterium strain AGL1. One binary Ti vector was assembled from pCAMBIA2301 with GUS as the reporter gene while the other binary Ti vector was assembled from pGreen0029 with GFP as the reporter gene. Two gene constructs containing specific gene fragments from Poncirus were used. The epicotyl explants were transformed by use of 1:1 mixture of AGL1 carrying the binary vectors separately. Shoots regenerated from transformed epicotyl explants on selective medium were micrografted onto 'Carrizo' seedlings. The GFP expression and the GUS activity in the leaves were also determined. Further confirmation by molecular analyses is currently underway.</div>
</front>
</TEI>
<inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0567-7572</s0>
</fA01>
<fA06><s2>738</s2>
</fA06>
<fA08 i1="01" i2="1" l="ENG"><s1>Agrobacterium-mediated transformation of citrus using two binary vectors</s1>
</fA08>
<fA09 i1="01" i2="1" l="ENG"><s1>Proceedings of the International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species : Daytona Beach, Florida, USA, October 10-14, 2005</s1>
</fA09>
<fA11 i1="01" i2="1"><s1>SONEJI (Jaya R.)</s1>
</fA11>
<fA11 i1="02" i2="1"><s1>CHUNXIAN CHEN</s1>
</fA11>
<fA11 i1="03" i2="1"><s1>NAGESWARA RAO (M.)</s1>
</fA11>
<fA11 i1="04" i2="1"><s1>SHU HUANG</s1>
</fA11>
<fA11 i1="05" i2="1"><s1>CHOI (Young A.)</s1>
</fA11>
<fA11 i1="06" i2="1"><s1>GMITTER (Fred G. JR)</s1>
</fA11>
<fA12 i1="01" i2="1"><s1>LITZ (Richard E.)</s1>
<s9>ed.</s9>
</fA12>
<fA12 i1="02" i2="1"><s1>SCORZA (Ralph)</s1>
<s9>ed.</s9>
</fA12>
<fA14 i1="01"><s1>University of Florida Citrus Research and Education Center 700 Experiment Station Road</s1>
<s2>Lake Alfred, FL 33850</s2>
<s3>USA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</fA14>
<fA18 i1="01" i2="1"><s1>International Society for Horticultural Science. Section on Tropical and Subtropical Fruits</s1>
<s3>INT</s3>
<s9>org-cong.</s9>
</fA18>
<fA20><s1>261-264</s1>
</fA20>
<fA21><s1>2007</s1>
</fA21>
<fA23 i1="01"><s0>ENG</s0>
</fA23>
<fA26 i1="01"><s0>978-90-6605-219-2</s0>
</fA26>
<fA43 i1="01"><s1>INIST</s1>
<s2>15963</s2>
<s5>354000153599050260</s5>
</fA43>
<fA44><s0>0000</s0>
<s1>© 2007 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>1/4 p.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>07-0530119</s0>
</fA47>
<fA60><s1>P</s1>
<s2>C</s2>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Acta horticulturae</s0>
</fA64>
<fA66 i1="01"><s0>NLD</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>In the present study, epicotyl explants of 'Midsweet' sweet orange (Citrus sinensis L. Osb.) were transformed using a mixture of two binary vectors individually introduced into Agrobacterium strain AGL1. One binary Ti vector was assembled from pCAMBIA2301 with GUS as the reporter gene while the other binary Ti vector was assembled from pGreen0029 with GFP as the reporter gene. Two gene constructs containing specific gene fragments from Poncirus were used. The epicotyl explants were transformed by use of 1:1 mixture of AGL1 carrying the binary vectors separately. Shoots regenerated from transformed epicotyl explants on selective medium were micrografted onto 'Carrizo' seedlings. The GFP expression and the GUS activity in the leaves were also determined. Further confirmation by molecular analyses is currently underway.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002A31C02A5B</s0>
</fC02>
<fC02 i1="02" i2="X"><s0>002A32D02B</s0>
</fC02>
<fC02 i1="03" i2="X"><s0>215</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>Agrume</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>Citrus fruit</s0>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>Agrios</s0>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Plante transgénique</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Transgenic plant</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Planta transgénica</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Transfection</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Transfection</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Transfección</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>Transfert génétique</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>Genetic transfer</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>Transferencia genética</s0>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Transformation génétique</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Genetic transformation</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Transformación genética</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Vecteur</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Vector</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Vector</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Système binaire</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Binary system</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Sistema binario</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Agrobacterium</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Agrobacterium</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Agrobacterium</s0>
<s2>NS</s2>
<s5>10</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>11</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Cultivar</s0>
<s5>12</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Cultivar</s0>
<s5>12</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Cultivar</s0>
<s5>12</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Plasmide Ti</s0>
<s5>14</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Ti Plasmid</s0>
<s5>14</s5>
<s6>«Ti» Plasmid</s6>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Plasmido Ti</s0>
<s5>14</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>β-Glucuronidase</s0>
<s2>FE</s2>
<s5>15</s5>
<s6>«β»-Glucuronidase</s6>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>β-Glucuronidase</s0>
<s2>FE</s2>
<s5>15</s5>
<s6>«β»-Glucuronidase</s6>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>β-Glucuronidase</s0>
<s2>FE</s2>
<s5>15</s5>
<s6>«β»-Glucuronidase</s6>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Protéine fluorescente verte</s0>
<s5>16</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Green fluorescent protein</s0>
<s5>16</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Proteína fluorescente verde</s0>
<s5>16</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Gène hybride</s0>
<s5>17</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Hybrid gene</s0>
<s5>17</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Gen híbrido</s0>
<s5>17</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Gène indicateur</s0>
<s5>18</s5>
</fC03>
<fC03 i1="15" i2="X" l="ENG"><s0>Reporter gene</s0>
<s5>18</s5>
</fC03>
<fC03 i1="15" i2="X" l="SPA"><s0>Gen marcador</s0>
<s5>18</s5>
</fC03>
<fC03 i1="16" i2="X" l="FRE"><s0>Gène structure</s0>
<s5>19</s5>
</fC03>
<fC03 i1="16" i2="X" l="ENG"><s0>Structural gene</s0>
<s5>19</s5>
</fC03>
<fC03 i1="16" i2="X" l="SPA"><s0>Gen estructura</s0>
<s5>19</s5>
</fC03>
<fC03 i1="17" i2="X" l="FRE"><s0>Gène gfp</s0>
<s4>INC</s4>
<s5>68</s5>
</fC03>
<fC03 i1="18" i2="X" l="FRE"><s0>Gène gus</s0>
<s4>INC</s4>
<s5>69</s5>
</fC03>
<fC03 i1="19" i2="X" l="FRE"><s0>cv Midsweet</s0>
<s4>INC</s4>
<s5>70</s5>
</fC03>
<fC03 i1="20" i2="X" l="FRE"><s0>Poncirus</s0>
<s4>INC</s4>
<s5>71</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Rhizobiaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Rhizobiaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Rhizobiaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Bactérie</s0>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Bacteria</s0>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Bacteria</s0>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="ENG"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="SPA"><s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="07" i2="X" l="FRE"><s0>O-Glycosidases</s0>
<s2>FE</s2>
<s6>«O»-Glycosidases</s6>
</fC07>
<fC07 i1="07" i2="X" l="ENG"><s0>O-Glycosidases</s0>
<s2>FE</s2>
<s6>«O»-Glycosidases</s6>
</fC07>
<fC07 i1="07" i2="X" l="SPA"><s0>O-Glycosidases</s0>
<s2>FE</s2>
<s6>«O»-Glycosidases</s6>
</fC07>
<fC07 i1="08" i2="X" l="FRE"><s0>Glycosidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="08" i2="X" l="ENG"><s0>Glycosidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="08" i2="X" l="SPA"><s0>Glycosidases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="09" i2="X" l="FRE"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="09" i2="X" l="ENG"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="09" i2="X" l="SPA"><s0>Hydrolases</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="10" i2="X" l="FRE"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="10" i2="X" l="ENG"><s0>Enzyme</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="10" i2="X" l="SPA"><s0>Enzima</s0>
<s2>FE</s2>
</fC07>
<fC07 i1="11" i2="X" l="FRE"><s0>Arbre fruitier</s0>
<s5>31</s5>
</fC07>
<fC07 i1="11" i2="X" l="ENG"><s0>Fruit tree</s0>
<s5>31</s5>
</fC07>
<fC07 i1="11" i2="X" l="SPA"><s0>Arbol frutal</s0>
<s5>31</s5>
</fC07>
<fC07 i1="12" i2="X" l="FRE"><s0>Plante fruitière</s0>
<s5>32</s5>
</fC07>
<fC07 i1="12" i2="X" l="ENG"><s0>Fruit crop</s0>
<s5>32</s5>
</fC07>
<fC07 i1="12" i2="X" l="SPA"><s0>Planta frutal</s0>
<s5>32</s5>
</fC07>
<fC07 i1="13" i2="X" l="FRE"><s0>Organisme génétiquement modifié</s0>
<s5>33</s5>
</fC07>
<fC07 i1="13" i2="X" l="ENG"><s0>Genetically modified organism</s0>
<s5>33</s5>
</fC07>
<fC07 i1="13" i2="X" l="SPA"><s0>Organismo modificado genéticamente</s0>
<s5>33</s5>
</fC07>
<fC07 i1="14" i2="X" l="FRE"><s0>Plante ligneuse</s0>
<s5>34</s5>
</fC07>
<fC07 i1="14" i2="X" l="ENG"><s0>Woody plant</s0>
<s5>34</s5>
</fC07>
<fC07 i1="14" i2="X" l="SPA"><s0>Planta leñosa</s0>
<s5>34</s5>
</fC07>
<fC07 i1="15" i2="X" l="FRE"><s0>Composé biochimique</s0>
<s5>35</s5>
</fC07>
<fC07 i1="15" i2="X" l="ENG"><s0>Biochemical compound</s0>
<s5>35</s5>
</fC07>
<fC07 i1="15" i2="X" l="SPA"><s0>Compuesto bioquímico</s0>
<s5>35</s5>
</fC07>
<fC07 i1="16" i2="X" l="FRE"><s0>Biotechnologie</s0>
<s5>36</s5>
</fC07>
<fC07 i1="16" i2="X" l="ENG"><s0>Biotechnology</s0>
<s5>36</s5>
</fC07>
<fC07 i1="16" i2="X" l="SPA"><s0>Biotecnología</s0>
<s5>36</s5>
</fC07>
<fC07 i1="17" i2="X" l="FRE"><s0>Génie génétique</s0>
<s5>37</s5>
</fC07>
<fC07 i1="17" i2="X" l="ENG"><s0>Genetic engineering</s0>
<s5>37</s5>
</fC07>
<fC07 i1="17" i2="X" l="SPA"><s0>Ingeniería genética</s0>
<s5>37</s5>
</fC07>
<fC07 i1="18" i2="X" l="FRE"><s0>Arboriculture</s0>
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</fC07>
<fC07 i1="18" i2="X" l="ENG"><s0>Arboriculture</s0>
<s5>64</s5>
</fC07>
<fC07 i1="18" i2="X" l="SPA"><s0>Arboricultura</s0>
<s5>64</s5>
</fC07>
<fC07 i1="19" i2="X" l="FRE"><s0>Horticulture</s0>
<s5>65</s5>
</fC07>
<fC07 i1="19" i2="X" l="ENG"><s0>Horticulture</s0>
<s5>65</s5>
</fC07>
<fC07 i1="19" i2="X" l="SPA"><s0>Horticultura</s0>
<s5>65</s5>
</fC07>
<fC07 i1="20" i2="X" l="FRE"><s0>Industrie fruits</s0>
<s5>66</s5>
</fC07>
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<s5>66</s5>
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<fC07 i1="20" i2="X" l="SPA"><s0>Industria frutas</s0>
<s5>66</s5>
</fC07>
<fC07 i1="21" i2="X" l="FRE"><s0>Bactérie Gram négatif</s0>
<s4>INC</s4>
<s5>84</s5>
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<fC07 i1="22" i2="X" l="FRE"><s0>Procaryote</s0>
<s4>INC</s4>
<s5>85</s5>
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<fN21><s1>344</s1>
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<pR><fA30 i1="01" i2="1" l="ENG"><s1>International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species</s1>
<s3>Daytona Beach FL USA</s3>
<s4>2005</s4>
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<server><NO>PASCAL 07-0530119 INIST</NO>
<ET>Agrobacterium-mediated transformation of citrus using two binary vectors</ET>
<AU>SONEJI (Jaya R.); CHUNXIAN CHEN; NAGESWARA RAO (M.); SHU HUANG; CHOI (Young A.); GMITTER (Fred G. JR); LITZ (Richard E.); SCORZA (Ralph)</AU>
<AF>University of Florida Citrus Research and Education Center 700 Experiment Station Road/Lake Alfred, FL 33850/Etats-Unis (1 aut., 2 aut., 3 aut., 4 aut., 5 aut., 6 aut.)</AF>
<DT>Publication en série; Congrès; Niveau analytique</DT>
<SO>Acta horticulturae; ISSN 0567-7572; Pays-Bas; Da. 2007; No. 738; Pp. 261-264; Bibl. 1/4 p.</SO>
<LA>Anglais</LA>
<EA>In the present study, epicotyl explants of 'Midsweet' sweet orange (Citrus sinensis L. Osb.) were transformed using a mixture of two binary vectors individually introduced into Agrobacterium strain AGL1. One binary Ti vector was assembled from pCAMBIA2301 with GUS as the reporter gene while the other binary Ti vector was assembled from pGreen0029 with GFP as the reporter gene. Two gene constructs containing specific gene fragments from Poncirus were used. The epicotyl explants were transformed by use of 1:1 mixture of AGL1 carrying the binary vectors separately. Shoots regenerated from transformed epicotyl explants on selective medium were micrografted onto 'Carrizo' seedlings. The GFP expression and the GUS activity in the leaves were also determined. Further confirmation by molecular analyses is currently underway.</EA>
<CC>002A31C02A5B; 002A32D02B; 215</CC>
<FD>Agrume; Plante transgénique; Transfection; Transfert génétique; Transformation génétique; Vecteur; Système binaire; Agrobacterium; Citrus sinensis; Cultivar; Plasmide Ti; β-Glucuronidase; Protéine fluorescente verte; Gène hybride; Gène indicateur; Gène structure; Gène gfp; Gène gus; cv Midsweet; Poncirus</FD>
<FG>Rhizobiaceae; Bactérie; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; O-Glycosidases; Glycosidases; Hydrolases; Enzyme; Arbre fruitier; Plante fruitière; Organisme génétiquement modifié; Plante ligneuse; Composé biochimique; Biotechnologie; Génie génétique; Arboriculture; Horticulture; Industrie fruits; Bactérie Gram négatif; Procaryote</FG>
<ED>Citrus fruit; Transgenic plant; Transfection; Genetic transfer; Genetic transformation; Vector; Binary system; Agrobacterium; Citrus sinensis; Cultivar; Ti Plasmid; β-Glucuronidase; Green fluorescent protein; Hybrid gene; Reporter gene; Structural gene</ED>
<EG>Rhizobiaceae; Bacteria; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; O-Glycosidases; Glycosidases; Hydrolases; Enzyme; Fruit tree; Fruit crop; Genetically modified organism; Woody plant; Biochemical compound; Biotechnology; Genetic engineering; Arboriculture; Horticulture; Fruit industry</EG>
<SD>Agrios; Planta transgénica; Transfección; Transferencia genética; Transformación genética; Vector; Sistema binario; Agrobacterium; Citrus sinensis; Cultivar; Plasmido Ti; β-Glucuronidase; Proteína fluorescente verde; Gen híbrido; Gen marcador; Gen estructura</SD>
<LO>INIST-15963.354000153599050260</LO>
<ID>07-0530119</ID>
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Agrobacterium-mediated transformation of citrus using two binary vectors

Agrobacterium-mediated transformation of citrus using two binary vectors

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