OrangerV1, PascalFrancis, Corpus, bibRecord, 000435

Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pêra

Identifieur interne : 000435 ( PascalFrancis/Corpus ); précédent : 000434; suivant : 000436

Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pêra

Auteurs : Rubia P. Purcino ; Camilo Lazaro Medina ; Daniel Martins ; Flavia Vischi Winck ; Eduardo Caruso Machado ; José Camilo Novello ; Marcos Antonio Machado ; Paulo Mazzafera

Source :

Journal of experimental botany [ 0022-0957 ] ; 2007.

RBID : Pascal:08-0012589

Descripteurs français

Pascal (Inist)
- Métabolisme, Stress, Aminoacide, Chlorose, Nitrate reductase, Polyamine, Peptidases, Xylème, Citrus sinensis.

English descriptors

KwdEn :
- Aminoacid, Chlorosis, Citrus sinensis, Metabolism, Nitrate reductase, Peptidases, Polyamine, Stress, Xylem.

Abstract

Xylella fastidiosa (Xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (Citrus sinensis L. Osb.) causing Pierce disease and citrus variegated chlorosis (CVC), respectively. The aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. 'Pêra' (C. sinensis L. Osbeck) infected with Xf. Plants were artificially infected and maintained in the greenhouse until they have developed clear disease symptoms. The content of nitrogen compounds and enzymes of the nitrogen metabolism and proteases in the xylem sap and leaves of diseased (DP) and uninfected healthy (HP) plants was studied. The activity of nitrate reductase in leaves did not change in DP, however, the activity of glutamine synthetase was significantly higher in these leaves. Although amino acid concentration was slightly higher in the xylem sap of DP, the level dropped drastically in the leaves. The protein contents were lower in the sap and in leaves of DP. DP and HP showed the same amino acid profiles, but different proportions were observed among them, mainly for asparagine, glutamine, and arginine. The polyamine putrescine was found in high concentrations only in DP. Protease activity was higher in leaves of DP while, in the xylem sap, activity was detected only in DP. Bidimensional electrophoresis showed a marked change in the protein pattern in DP. Five differentially expressed proteins were identified (2 from HP and 3 from DP), but none showed similarity with the genomic (translated) and proteomic database of Xf, but do show similarity with the proteins thaumatin, mucin, peroxidase, ABC-transporter, and strictosidine synthase. These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

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A11	`01`	`1`		`@1 PURCINO (Rubia P.)`
A11	`02`	`1`		`@1 MEDINA (Camilo Lazaro)`
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A11	`05`	`1`		`@1 CARUSO MACHADO (Eduardo)`
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A14	`01`			`@1 Departamento de Fisiologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas , CP 6109 @2 13083-970, Campinas, SP @3 BRA @Z 1 aut. @Z 8 aut.`
A14	`02`			`@1 Universidade Fédéral Rural do Rio de Janeiro, Km 7 Rodovia BR 465 @2 23850-000, Seropédica, RJ @3 BRA @Z 1 aut.`
A14	`03`			`@1 Conplant, Rua Francisco Andreo Aledo, 22 @2 13084-200, Campinas, SP @3 BRA @Z 2 aut.`
A14	`04`			`@1 Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas , CP 6109 @2 13083-970, Campinas, SP @3 BRA @Z 3 aut. @Z 4 aut. @Z 6 aut.`
A14	`05`			`@1 Instituto Agronômico de Campinas, Centro de Ecofisiologia e Biofísica, Av. Barão de Itapura 1481, CP 28 @2 13012-970, Campinas, SP @3 BRA @Z 5 aut.`
A14	`06`			`@1 Instituto Agronômico de Campinas, Centro APTA Citros Sylvio Moreira, CP 04 @2 13490-970 , Cordeirópolis, SP @3 BRA @Z 7 aut.`
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C01	`01`		`ENG`	@0 Xylella fastidiosa (Xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (Citrus sinensis L. Osb.) causing Pierce disease and citrus variegated chlorosis (CVC), respectively. The aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. 'Pêra' (C. sinensis L. Osbeck) infected with Xf. Plants were artificially infected and maintained in the greenhouse until they have developed clear disease symptoms. The content of nitrogen compounds and enzymes of the nitrogen metabolism and proteases in the xylem sap and leaves of diseased (DP) and uninfected healthy (HP) plants was studied. The activity of nitrate reductase in leaves did not change in DP, however, the activity of glutamine synthetase was significantly higher in these leaves. Although amino acid concentration was slightly higher in the xylem sap of DP, the level dropped drastically in the leaves. The protein contents were lower in the sap and in leaves of DP. DP and HP showed the same amino acid profiles, but different proportions were observed among them, mainly for asparagine, glutamine, and arginine. The polyamine putrescine was found in high concentrations only in DP. Protease activity was higher in leaves of DP while, in the xylem sap, activity was detected only in DP. Bidimensional electrophoresis showed a marked change in the protein pattern in DP. Five differentially expressed proteins were identified (2 from HP and 3 from DP), but none showed similarity with the genomic (translated) and proteomic database of Xf, but do show similarity with the proteins thaumatin, mucin, peroxidase, ABC-transporter, and strictosidine synthase. These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant.
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C03	`09`	`X`	`SPA`	`@0 Citrus sinensis @2 NS @5 10`
C07	`01`	`X`	`FRE`	`@0 Oxidoreductases @2 FE`
C07	`01`	`X`	`ENG`	`@0 Oxidoreductases @2 FE`
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C07	`02`	`X`	`FRE`	`@0 Enzyme @2 FE`
C07	`02`	`X`	`ENG`	`@0 Enzyme @2 FE`
C07	`02`	`X`	`SPA`	`@0 Enzima @2 FE`
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C07	`03`	`X`	`SPA`	`@0 Hydrolases @2 FE`
C07	`04`	`X`	`FRE`	`@0 Rutaceae @2 NS`
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C07	`05`	`X`	`FRE`	`@0 Dicotyledones @2 NS`
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N21				`@1 009`
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Format Inist (serveur)

NO :	PASCAL 08-0012589 INIST
ET :	Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pêra
AU :	PURCINO (Rubia P.); MEDINA (Camilo Lazaro); MARTINS (Daniel); VISCHI WINCK (Flavia); CARUSO MACHADO (Eduardo); CAMILO NOVELLO (José); MACHADO (Marcos Antonio); MAZZAFERA (Paulo)
AF :	Departamento de Fisiologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas , CP 6109/13083-970, Campinas, SP/Brésil (1 aut., 8 aut.); Universidade Fédéral Rural do Rio de Janeiro, Km 7 Rodovia BR 465/23850-000, Seropédica, RJ/Brésil (1 aut.); Conplant, Rua Francisco Andreo Aledo, 22/13084-200, Campinas, SP/Brésil (2 aut.); Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas , CP 6109/13083-970, Campinas, SP/Brésil (3 aut., 4 aut., 6 aut.); Instituto Agronômico de Campinas, Centro de Ecofisiologia e Biofísica, Av. Barão de Itapura 1481, CP 28/13012-970, Campinas, SP/Brésil (5 aut.); Instituto Agronômico de Campinas, Centro APTA Citros Sylvio Moreira, CP 04/13490-970 , Cordeirópolis, SP/Brésil (7 aut.)
DT :	Publication en série; Papier de recherche; Niveau analytique
SO :	Journal of experimental botany; ISSN 0022-0957; Coden JEBOA6; Royaume-Uni; Da. 2007; Vol. 58; No. 11; Pp. 2733-2744; Bibl. 2 p.1/4
LA :	Anglais
EA :	Xylella fastidiosa (Xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (Citrus sinensis L. Osb.) causing Pierce disease and citrus variegated chlorosis (CVC), respectively. The aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. 'Pêra' (C. sinensis L. Osbeck) infected with Xf. Plants were artificially infected and maintained in the greenhouse until they have developed clear disease symptoms. The content of nitrogen compounds and enzymes of the nitrogen metabolism and proteases in the xylem sap and leaves of diseased (DP) and uninfected healthy (HP) plants was studied. The activity of nitrate reductase in leaves did not change in DP, however, the activity of glutamine synthetase was significantly higher in these leaves. Although amino acid concentration was slightly higher in the xylem sap of DP, the level dropped drastically in the leaves. The protein contents were lower in the sap and in leaves of DP. DP and HP showed the same amino acid profiles, but different proportions were observed among them, mainly for asparagine, glutamine, and arginine. The polyamine putrescine was found in high concentrations only in DP. Protease activity was higher in leaves of DP while, in the xylem sap, activity was detected only in DP. Bidimensional electrophoresis showed a marked change in the protein pattern in DP. Five differentially expressed proteins were identified (2 from HP and 3 from DP), but none showed similarity with the genomic (translated) and proteomic database of Xf, but do show similarity with the proteins thaumatin, mucin, peroxidase, ABC-transporter, and strictosidine synthase. These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant.
CC :	002A10E02
FD :	Métabolisme; Stress; Aminoacide; Chlorose; Nitrate reductase; Polyamine; Peptidases; Xylème; Citrus sinensis
FG :	Oxidoreductases; Enzyme; Hydrolases; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta
ED :	Metabolism; Stress; Aminoacid; Chlorosis; Nitrate reductase; Polyamine; Peptidases; Xylem; Citrus sinensis
EG :	Oxidoreductases; Enzyme; Hydrolases; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta
SD :	Metabolismo; Estrés; Aminoácido; Clorosis; Nitrate reductase; Poliamina; Peptidases; Xilema; Citrus sinensis
LO :	INIST-6923.354000173537160020
ID :	08-0012589

Links to Exploration step

Pascal:08-0012589

Le document en format XML

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<author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
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<author><name sortKey="Mazzafera, Paulo" sort="Mazzafera, Paulo" uniqKey="Mazzafera P" first="Paulo" last="Mazzafera">Paulo Mazzafera</name>
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<series><title level="j" type="main">Journal of experimental botany</title>
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<term>Metabolism</term>
<term>Nitrate reductase</term>
<term>Peptidases</term>
<term>Polyamine</term>
<term>Stress</term>
<term>Xylem</term>
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<term>Stress</term>
<term>Aminoacide</term>
<term>Chlorose</term>
<term>Nitrate reductase</term>
<term>Polyamine</term>
<term>Peptidases</term>
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<front><div type="abstract" xml:lang="en">Xylella fastidiosa (Xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (Citrus sinensis L. Osb.) causing Pierce disease and citrus variegated chlorosis (CVC), respectively. The aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. 'Pêra' (C. sinensis L. Osbeck) infected with Xf. Plants were artificially infected and maintained in the greenhouse until they have developed clear disease symptoms. The content of nitrogen compounds and enzymes of the nitrogen metabolism and proteases in the xylem sap and leaves of diseased (DP) and uninfected healthy (HP) plants was studied. The activity of nitrate reductase in leaves did not change in DP, however, the activity of glutamine synthetase was significantly higher in these leaves. Although amino acid concentration was slightly higher in the xylem sap of DP, the level dropped drastically in the leaves. The protein contents were lower in the sap and in leaves of DP. DP and HP showed the same amino acid profiles, but different proportions were observed among them, mainly for asparagine, glutamine, and arginine. The polyamine putrescine was found in high concentrations only in DP. Protease activity was higher in leaves of DP while, in the xylem sap, activity was detected only in DP. Bidimensional electrophoresis showed a marked change in the protein pattern in DP. Five differentially expressed proteins were identified (2 from HP and 3 from DP), but none showed similarity with the genomic (translated) and proteomic database of Xf, but do show similarity with the proteins thaumatin, mucin, peroxidase, ABC-transporter, and strictosidine synthase. These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant.</div>
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<fA14 i1="05"><s1>Instituto Agronômico de Campinas, Centro de Ecofisiologia e Biofísica, Av. Barão de Itapura 1481, CP 28</s1>
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<s3>BRA</s3>
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<fC01 i1="01" l="ENG"><s0>Xylella fastidiosa (Xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (Citrus sinensis L. Osb.) causing Pierce disease and citrus variegated chlorosis (CVC), respectively. The aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. 'Pêra' (C. sinensis L. Osbeck) infected with Xf. Plants were artificially infected and maintained in the greenhouse until they have developed clear disease symptoms. The content of nitrogen compounds and enzymes of the nitrogen metabolism and proteases in the xylem sap and leaves of diseased (DP) and uninfected healthy (HP) plants was studied. The activity of nitrate reductase in leaves did not change in DP, however, the activity of glutamine synthetase was significantly higher in these leaves. Although amino acid concentration was slightly higher in the xylem sap of DP, the level dropped drastically in the leaves. The protein contents were lower in the sap and in leaves of DP. DP and HP showed the same amino acid profiles, but different proportions were observed among them, mainly for asparagine, glutamine, and arginine. The polyamine putrescine was found in high concentrations only in DP. Protease activity was higher in leaves of DP while, in the xylem sap, activity was detected only in DP. Bidimensional electrophoresis showed a marked change in the protein pattern in DP. Five differentially expressed proteins were identified (2 from HP and 3 from DP), but none showed similarity with the genomic (translated) and proteomic database of Xf, but do show similarity with the proteins thaumatin, mucin, peroxidase, ABC-transporter, and strictosidine synthase. These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant.</s0>
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<ET>Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pêra</ET>
<AU>PURCINO (Rubia P.); MEDINA (Camilo Lazaro); MARTINS (Daniel); VISCHI WINCK (Flavia); CARUSO MACHADO (Eduardo); CAMILO NOVELLO (José); MACHADO (Marcos Antonio); MAZZAFERA (Paulo)</AU>
<AF>Departamento de Fisiologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas , CP 6109/13083-970, Campinas, SP/Brésil (1 aut., 8 aut.); Universidade Fédéral Rural do Rio de Janeiro, Km 7 Rodovia BR 465/23850-000, Seropédica, RJ/Brésil (1 aut.); Conplant, Rua Francisco Andreo Aledo, 22/13084-200, Campinas, SP/Brésil (2 aut.); Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas , CP 6109/13083-970, Campinas, SP/Brésil (3 aut., 4 aut., 6 aut.); Instituto Agronômico de Campinas, Centro de Ecofisiologia e Biofísica, Av. Barão de Itapura 1481, CP 28/13012-970, Campinas, SP/Brésil (5 aut.); Instituto Agronômico de Campinas, Centro APTA Citros Sylvio Moreira, CP 04/13490-970 , Cordeirópolis, SP/Brésil (7 aut.)</AF>
<DT>Publication en série; Papier de recherche; Niveau analytique</DT>
<SO>Journal of experimental botany; ISSN 0022-0957; Coden JEBOA6; Royaume-Uni; Da. 2007; Vol. 58; No. 11; Pp. 2733-2744; Bibl. 2 p.1/4</SO>
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<EA>Xylella fastidiosa (Xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (Citrus sinensis L. Osb.) causing Pierce disease and citrus variegated chlorosis (CVC), respectively. The aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. 'Pêra' (C. sinensis L. Osbeck) infected with Xf. Plants were artificially infected and maintained in the greenhouse until they have developed clear disease symptoms. The content of nitrogen compounds and enzymes of the nitrogen metabolism and proteases in the xylem sap and leaves of diseased (DP) and uninfected healthy (HP) plants was studied. The activity of nitrate reductase in leaves did not change in DP, however, the activity of glutamine synthetase was significantly higher in these leaves. Although amino acid concentration was slightly higher in the xylem sap of DP, the level dropped drastically in the leaves. The protein contents were lower in the sap and in leaves of DP. DP and HP showed the same amino acid profiles, but different proportions were observed among them, mainly for asparagine, glutamine, and arginine. The polyamine putrescine was found in high concentrations only in DP. Protease activity was higher in leaves of DP while, in the xylem sap, activity was detected only in DP. Bidimensional electrophoresis showed a marked change in the protein pattern in DP. Five differentially expressed proteins were identified (2 from HP and 3 from DP), but none showed similarity with the genomic (translated) and proteomic database of Xf, but do show similarity with the proteins thaumatin, mucin, peroxidase, ABC-transporter, and strictosidine synthase. These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant.</EA>
<CC>002A10E02</CC>
<FD>Métabolisme; Stress; Aminoacide; Chlorose; Nitrate reductase; Polyamine; Peptidases; Xylème; Citrus sinensis</FD>
<FG>Oxidoreductases; Enzyme; Hydrolases; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta</FG>
<ED>Metabolism; Stress; Aminoacid; Chlorosis; Nitrate reductase; Polyamine; Peptidases; Xylem; Citrus sinensis</ED>
<EG>Oxidoreductases; Enzyme; Hydrolases; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta</EG>
<SD>Metabolismo; Estrés; Aminoácido; Clorosis; Nitrate reductase; Poliamina; Peptidases; Xilema; Citrus sinensis</SD>
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Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pêra

Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pêra

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