OrangerV1, Ncbi, Checkpoint, bibRecord, 000827

An Evaluation of the Basis and Consequences of a Stay-Green Mutation in the navel negra Citrus Mutant Using Transcriptomic and Proteomic Profiling and Metabolite Analysis1[W]

Identifieur interne : 000827 ( Ncbi/Checkpoint ); précédent : 000826; suivant : 000828

An Evaluation of the Basis and Consequences of a Stay-Green Mutation in the navel negra Citrus Mutant Using Transcriptomic and Proteomic Profiling and Metabolite Analysis1[W]

Auteurs : Enriqueta Al S ; María Roca ; Domingo José Iglesias ; Maria Isabel Mínguez-Mosquera ; Cynthia Maria Borges Damasceno ; Theodore William Thannhauser ; Jocelyn Kenneth Campbell Rose ; Manuel Tal N ; Manuel Cerc S

Source :

Plant Physiology [ 0032-0889 ] ; 2008.

RBID : PMC:2442528

English descriptors

KwdEn :
- Arabidopsis Proteins, Carboxylic Ester Hydrolases (metabolism), Chlorophyll (metabolism), Citrus sinensis (genetics), Citrus sinensis (metabolism), Electrophoresis, Gel, Two-Dimensional, Ethylenes (metabolism), Fruit (metabolism), Gene Expression, Gene Expression Profiling, Molecular Sequence Data, Mutation, Oligonucleotide Array Sequence Analysis, Plant Proteins (metabolism), Proteome.
MESH :
- chemical , metabolism : Carboxylic Ester Hydrolases, Chlorophyll, Ethylenes, Plant Proteins.
- chemical : Arabidopsis Proteins, Proteome.
- genetics : Citrus sinensis.
- metabolism : Citrus sinensis, Fruit.
- Electrophoresis, Gel, Two-Dimensional, Gene Expression, Gene Expression Profiling, Molecular Sequence Data, Mutation, Oligonucleotide Array Sequence Analysis.

Abstract

A Citrus sinensis spontaneous mutant, navel negra (nan), produces fruit with an abnormal brown-colored flavedo during ripening. Analysis of pigment composition in the wild-type and nan flavedo suggested that typical ripening-related chlorophyll (Chl) degradation, but not carotenoid biosynthesis, was impaired in the mutant, identifying nan as a type C stay-green mutant. nan exhibited normal expression of Chl biosynthetic and catabolic genes and chlorophyllase activity but no accumulation of dephytylated Chl compounds during ripening, suggesting that the mutation is not related to a lesion in any of the principal enzymatic steps in Chl catabolism. Transcript profiling using a citrus microarray indicated that a citrus ortholog of a number of SGR (for STAY-GREEN) genes was expressed at substantially lower levels in nan, both prior to and during ripening. However, the pattern of catabolite accumulation and SGR sequence analysis suggested that the nan mutation is distinct from those in previously described stay-green mutants and is associated with an upstream regulatory step, rather than directly influencing a specific component of Chl catabolism. Transcriptomic and comparative proteomic profiling further indicated that the nan mutation resulted in the suppressed expression of numerous photosynthesis-related genes and in the induction of genes that are associated with oxidative stress. These data, along with metabolite analyses, suggest that nan fruit employ a number of molecular mechanisms to compensate for the elevated Chl levels and associated photooxidative stress.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442528

DOI: 10.1104/pp.108.119917
PubMed: 18467459
PubMed Central: 2442528

Affiliations:

Links to Exploration step

PMC:2442528

Le document en format XML

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 Citrus Mutant Using Transcriptomic and Proteomic Profiling and Metabolite Analysis<xref ref-type="fn" rid="fn1">1</xref>
<xref ref-type="fn" rid="fn3">[W]</xref>
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<author><name sortKey="Al S, Enriqueta" sort="Al S, Enriqueta" uniqKey="Al S E" first="Enriqueta" last="Al S">Enriqueta Al S</name>
</author>
<author><name sortKey="Roca, Maria" sort="Roca, Maria" uniqKey="Roca M" first="María" last="Roca">María Roca</name>
</author>
<author><name sortKey="Iglesias, Domingo Jose" sort="Iglesias, Domingo Jose" uniqKey="Iglesias D" first="Domingo José" last="Iglesias">Domingo José Iglesias</name>
</author>
<author><name sortKey="Minguez Mosquera, Maria Isabel" sort="Minguez Mosquera, Maria Isabel" uniqKey="Minguez Mosquera M" first="Maria Isabel" last="Mínguez-Mosquera">Maria Isabel Mínguez-Mosquera</name>
</author>
<author><name sortKey="Damasceno, Cynthia Maria Borges" sort="Damasceno, Cynthia Maria Borges" uniqKey="Damasceno C" first="Cynthia Maria Borges" last="Damasceno">Cynthia Maria Borges Damasceno</name>
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<author><name sortKey="Thannhauser, Theodore William" sort="Thannhauser, Theodore William" uniqKey="Thannhauser T" first="Theodore William" last="Thannhauser">Theodore William Thannhauser</name>
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<author><name sortKey="Rose, Jocelyn Kenneth Campbell" sort="Rose, Jocelyn Kenneth Campbell" uniqKey="Rose J" first="Jocelyn Kenneth Campbell" last="Rose">Jocelyn Kenneth Campbell Rose</name>
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<author><name sortKey="Tal N, Manuel" sort="Tal N, Manuel" uniqKey="Tal N M" first="Manuel" last="Tal N">Manuel Tal N</name>
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<author><name sortKey="Cerc S, Manuel" sort="Cerc S, Manuel" uniqKey="Cerc S M" first="Manuel" last="Cerc S">Manuel Cerc S</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">An Evaluation of the Basis and Consequences of a Stay-Green Mutation in the <italic>navel negra</italic>
 Citrus Mutant Using Transcriptomic and Proteomic Profiling and Metabolite Analysis<xref ref-type="fn" rid="fn1">1</xref>
<xref ref-type="fn" rid="fn3">[W]</xref>
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<author><name sortKey="Al S, Enriqueta" sort="Al S, Enriqueta" uniqKey="Al S E" first="Enriqueta" last="Al S">Enriqueta Al S</name>
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<author><name sortKey="Roca, Maria" sort="Roca, Maria" uniqKey="Roca M" first="María" last="Roca">María Roca</name>
</author>
<author><name sortKey="Iglesias, Domingo Jose" sort="Iglesias, Domingo Jose" uniqKey="Iglesias D" first="Domingo José" last="Iglesias">Domingo José Iglesias</name>
</author>
<author><name sortKey="Minguez Mosquera, Maria Isabel" sort="Minguez Mosquera, Maria Isabel" uniqKey="Minguez Mosquera M" first="Maria Isabel" last="Mínguez-Mosquera">Maria Isabel Mínguez-Mosquera</name>
</author>
<author><name sortKey="Damasceno, Cynthia Maria Borges" sort="Damasceno, Cynthia Maria Borges" uniqKey="Damasceno C" first="Cynthia Maria Borges" last="Damasceno">Cynthia Maria Borges Damasceno</name>
</author>
<author><name sortKey="Thannhauser, Theodore William" sort="Thannhauser, Theodore William" uniqKey="Thannhauser T" first="Theodore William" last="Thannhauser">Theodore William Thannhauser</name>
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<author><name sortKey="Rose, Jocelyn Kenneth Campbell" sort="Rose, Jocelyn Kenneth Campbell" uniqKey="Rose J" first="Jocelyn Kenneth Campbell" last="Rose">Jocelyn Kenneth Campbell Rose</name>
</author>
<author><name sortKey="Tal N, Manuel" sort="Tal N, Manuel" uniqKey="Tal N M" first="Manuel" last="Tal N">Manuel Tal N</name>
</author>
<author><name sortKey="Cerc S, Manuel" sort="Cerc S, Manuel" uniqKey="Cerc S M" first="Manuel" last="Cerc S">Manuel Cerc S</name>
</author>
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<series><title level="j">Plant Physiology</title>
<idno type="ISSN">0032-0889</idno>
<idno type="eISSN">1532-2548</idno>
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<term>Citrus sinensis (metabolism)</term>
<term>Electrophoresis, Gel, Two-Dimensional</term>
<term>Ethylenes (metabolism)</term>
<term>Fruit (metabolism)</term>
<term>Gene Expression</term>
<term>Gene Expression Profiling</term>
<term>Molecular Sequence Data</term>
<term>Mutation</term>
<term>Oligonucleotide Array Sequence Analysis</term>
<term>Plant Proteins (metabolism)</term>
<term>Proteome</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carboxylic Ester Hydrolases</term>
<term>Chlorophyll</term>
<term>Ethylenes</term>
<term>Plant Proteins</term>
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<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Arabidopsis Proteins</term>
<term>Proteome</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus sinensis</term>
<term>Fruit</term>
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<term>Gene Expression</term>
<term>Gene Expression Profiling</term>
<term>Molecular Sequence Data</term>
<term>Mutation</term>
<term>Oligonucleotide Array Sequence Analysis</term>
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<front><div type="abstract" xml:lang="en"><p>A <italic>Citrus sinensis</italic>
 spontaneous mutant, <italic>navel negra</italic>
 (<italic>nan</italic>
), produces fruit with an abnormal brown-colored flavedo during ripening. Analysis of pigment composition in the wild-type and <italic>nan</italic>
 flavedo suggested that typical ripening-related chlorophyll (Chl) degradation, but not carotenoid biosynthesis, was impaired in the mutant, identifying <italic>nan</italic>
 as a type C stay-green mutant. <italic>nan</italic>
 exhibited normal expression of Chl biosynthetic and catabolic genes and chlorophyllase activity but no accumulation of dephytylated Chl compounds during ripening, suggesting that the mutation is not related to a lesion in any of the principal enzymatic steps in Chl catabolism. Transcript profiling using a citrus microarray indicated that a citrus ortholog of a number of <italic>SGR</italic>
 (for <italic>STAY-GREEN</italic>
) genes was expressed at substantially lower levels in <italic>nan</italic>
, both prior to and during ripening. However, the pattern of catabolite accumulation and <italic>SGR</italic>
 sequence analysis suggested that the <italic>nan</italic>
 mutation is distinct from those in previously described stay-green mutants and is associated with an upstream regulatory step, rather than directly influencing a specific component of Chl catabolism. Transcriptomic and comparative proteomic profiling further indicated that the <italic>nan</italic>
 mutation resulted in the suppressed expression of numerous photosynthesis-related genes and in the induction of genes that are associated with oxidative stress. These data, along with metabolite analyses, suggest that <italic>nan</italic>
 fruit employ a number of molecular mechanisms to compensate for the elevated Chl levels and associated photooxidative stress.</p>
</div>
</front>
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<affiliations><list></list>
<tree><noCountry><name sortKey="Al S, Enriqueta" sort="Al S, Enriqueta" uniqKey="Al S E" first="Enriqueta" last="Al S">Enriqueta Al S</name>
<name sortKey="Cerc S, Manuel" sort="Cerc S, Manuel" uniqKey="Cerc S M" first="Manuel" last="Cerc S">Manuel Cerc S</name>
<name sortKey="Damasceno, Cynthia Maria Borges" sort="Damasceno, Cynthia Maria Borges" uniqKey="Damasceno C" first="Cynthia Maria Borges" last="Damasceno">Cynthia Maria Borges Damasceno</name>
<name sortKey="Iglesias, Domingo Jose" sort="Iglesias, Domingo Jose" uniqKey="Iglesias D" first="Domingo José" last="Iglesias">Domingo José Iglesias</name>
<name sortKey="Minguez Mosquera, Maria Isabel" sort="Minguez Mosquera, Maria Isabel" uniqKey="Minguez Mosquera M" first="Maria Isabel" last="Mínguez-Mosquera">Maria Isabel Mínguez-Mosquera</name>
<name sortKey="Roca, Maria" sort="Roca, Maria" uniqKey="Roca M" first="María" last="Roca">María Roca</name>
<name sortKey="Rose, Jocelyn Kenneth Campbell" sort="Rose, Jocelyn Kenneth Campbell" uniqKey="Rose J" first="Jocelyn Kenneth Campbell" last="Rose">Jocelyn Kenneth Campbell Rose</name>
<name sortKey="Tal N, Manuel" sort="Tal N, Manuel" uniqKey="Tal N M" first="Manuel" last="Tal N">Manuel Tal N</name>
<name sortKey="Thannhauser, Theodore William" sort="Thannhauser, Theodore William" uniqKey="Thannhauser T" first="Theodore William" last="Thannhauser">Theodore William Thannhauser</name>
</noCountry>
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An Evaluation of the Basis and Consequences of a Stay-Green Mutation in the navel negra Citrus Mutant Using Transcriptomic and Proteomic Profiling and Metabolite Analysis1[W]

An Evaluation of the Basis and Consequences of a Stay-Green Mutation in the navel negra Citrus Mutant Using Transcriptomic and Proteomic Profiling and Metabolite Analysis1[W]

Source :

English descriptors

Abstract

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