Application of diode array detection with a C-30 reversed phase column for the separation and identification of saponified orange juice carotenoids
Identifieur interne : 003508 ( Main/Exploration ); précédent : 003507; suivant : 003509Application of diode array detection with a C-30 reversed phase column for the separation and identification of saponified orange juice carotenoids
Auteurs : R. Rouseff [États-Unis] ; L. Raley [États-Unis] ; H.-J. Hofsommer [Allemagne]Source :
- Journal of agricultural and food chemistry [ 0021-8561 ] ; 1996.
Descripteurs français
- Pascal (Inist)
English descriptors
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Abstract
Thirty-nine carotenoid pigments in saponified orange (Citrus sinensis) juice were separated using a water, methanol, methyl-tert-butyl ether gradient on a non-endcapped C-30 reversed phase column. Pigments were extracted using the International Fruit Juice Union method for orange juice carotenoids, which employs precipitation with Carrez reagent and resolubilizing with acetone. Chromatographic resolution, Rs, between lutein and zeaxanthin was 2.9. Pigments were identified on the basis of diode array spectral characteristics, retention times, and relative elution order compared to authentic standards and literature values. An examination of the diode array data from the chromatographically resolved peaks indicated that the most useful information could be obtained from monitoring the chromatographic effluent at 350, 430, and 486 nm. More carotenoid peaks were detected at 430 nm, but greater selectivity for several carotenoids was obtained at 350 and 486 nm. At 430 nm the six largest carotenoid peaks observed in orange juice consist of auroxanthin A, mutatoxanthin A, mutatoxanthin B, lutein, zeaxanthin, and isolutein. Whereas open column and thin-layer chromatography required days, the separation and identification of saponified orange juice carotenoids can now be accomplished within 40 min.
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Raley</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research and Education Center, University of Florida</s1><s2>Lake Alfred, Florida 33850</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Lake Alfred, Florida 33850</wicri:noRegion></affiliation></author><author><name sortKey="Hofsommer, H J" sort="Hofsommer, H J" uniqKey="Hofsommer H" first="H.-J." last="Hofsommer">H.-J. Hofsommer</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Gesellschaft für Lebensmittel-Forschung, Landgrafenstrasse 16</s1><s2>10787 Berlin</s2><s3>DEU</s3><sZ>3 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">96-0438785</idno><date when="1996">1996</date><idno type="stanalyst">PASCAL 96-0438785 INIST</idno><idno type="RBID">Pascal:96-0438785</idno><idno type="wicri:Area/PascalFrancis/Corpus">000B57</idno><idno type="wicri:Area/PascalFrancis/Curation">001352</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000B67</idno><idno type="wicri:doubleKey">0021-8561:1996:Rouseff R:application:of:diode</idno><idno type="wicri:Area/Main/Merge">003769</idno><idno type="wicri:Area/Main/Curation">003508</idno><idno type="wicri:Area/Main/Exploration">003508</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Application of diode array detection with a C-30 reversed phase column for the separation and identification of saponified orange juice carotenoids</title><author><name sortKey="Rouseff, R" sort="Rouseff, R" uniqKey="Rouseff R" first="R." last="Rouseff">R. Rouseff</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research and Education Center, University of Florida</s1><s2>Lake Alfred, Florida 33850</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Lake Alfred, Florida 33850</wicri:noRegion></affiliation></author><author><name sortKey="Raley, L" sort="Raley, L" uniqKey="Raley L" first="L." last="Raley">L. Raley</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research and Education Center, University of Florida</s1><s2>Lake Alfred, Florida 33850</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Lake Alfred, Florida 33850</wicri:noRegion></affiliation></author><author><name sortKey="Hofsommer, H J" sort="Hofsommer, H J" uniqKey="Hofsommer H" first="H.-J." last="Hofsommer">H.-J. Hofsommer</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Gesellschaft für Lebensmittel-Forschung, Landgrafenstrasse 16</s1><s2>10787 Berlin</s2><s3>DEU</s3><sZ>3 aut.</sZ></inist:fA14><country>Allemagne</country><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of agricultural and food chemistry</title><title level="j" type="abbreviated">J. agric. food chem.</title><idno type="ISSN">0021-8561</idno><imprint><date when="1996">1996</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of agricultural and food chemistry</title><title level="j" type="abbreviated">J. agric. food chem.</title><idno type="ISSN">0021-8561</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Analytical method</term><term>Chemical composition</term><term>Cryptoxanthin</term><term>Identification</term><term>Lutein</term><term>Orange juice</term><term>Pigments</term><term>Reversed phase chromatography</term><term>Separation</term><term>Zeaxanthin</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Cryptoxanthine</term><term>Composition chimique</term><term>Pigment</term><term>Méthode analytique</term><term>Jus d'orange</term><term>Chromatographie phase inverse</term><term>Séparation</term><term>Identification</term><term>Lutéine</term><term>Zéaxanthine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Thirty-nine carotenoid pigments in saponified orange (Citrus sinensis) juice were separated using a water, methanol, methyl-tert-butyl ether gradient on a non-endcapped C-30 reversed phase column. Pigments were extracted using the International Fruit Juice Union method for orange juice carotenoids, which employs precipitation with Carrez reagent and resolubilizing with acetone. Chromatographic resolution, R<sub>s</sub>, between lutein and zeaxanthin was 2.9. Pigments were identified on the basis of diode array spectral characteristics, retention times, and relative elution order compared to authentic standards and literature values. An examination of the diode array data from the chromatographically resolved peaks indicated that the most useful information could be obtained from monitoring the chromatographic effluent at 350, 430, and 486 nm. More carotenoid peaks were detected at 430 nm, but greater selectivity for several carotenoids was obtained at 350 and 486 nm. At 430 nm the six largest carotenoid peaks observed in orange juice consist of auroxanthin A, mutatoxanthin A, mutatoxanthin B, lutein, zeaxanthin, and isolutein. Whereas open column and thin-layer chromatography required days, the separation and identification of saponified orange juice carotenoids can now be accomplished within 40 min.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>États-Unis</li></country><region><li>Berlin</li></region><settlement><li>Berlin</li></settlement></list><tree><country name="États-Unis"><noRegion><name sortKey="Rouseff, R" sort="Rouseff, R" uniqKey="Rouseff R" first="R." last="Rouseff">R. Rouseff</name></noRegion><name sortKey="Raley, L" sort="Raley, L" uniqKey="Raley L" first="L." last="Raley">L. Raley</name></country><country name="Allemagne"><region name="Berlin"><name sortKey="Hofsommer, H J" sort="Hofsommer, H J" uniqKey="Hofsommer H" first="H.-J." last="Hofsommer">H.-J. Hofsommer</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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