OrangerV1, PascalFrancis, Corpus, bibRecord, 000B57

Application of diode array detection with a C-30 reversed phase column for the separation and identification of saponified orange juice carotenoids

Identifieur interne : 000B57 ( PascalFrancis/Corpus ); précédent : 000B56; suivant : 000B58

Application of diode array detection with a C-30 reversed phase column for the separation and identification of saponified orange juice carotenoids

Auteurs : R. Rouseff ; L. Raley ; H.-J. Hofsommer

Source :

Journal of agricultural and food chemistry [ 0021-8561 ] ; 1996.

RBID : Pascal:96-0438785

Descripteurs français

Pascal (Inist)
- Cryptoxanthine, Composition chimique, Pigment, Méthode analytique, Jus d'orange, Chromatographie phase inverse, Séparation, Identification, Lutéine, Zéaxanthine.

English descriptors

KwdEn :
- Analytical method, Chemical composition, Cryptoxanthin, Identification, Lutein, Orange juice, Pigments, Reversed phase chromatography, Separation, Zeaxanthin.

Abstract

Thirty-nine carotenoid pigments in saponified orange (Citrus sinensis) juice were separated using a water, methanol, methyl-tert-butyl ether gradient on a non-endcapped C-30 reversed phase column. Pigments were extracted using the International Fruit Juice Union method for orange juice carotenoids, which employs precipitation with Carrez reagent and resolubilizing with acetone. Chromatographic resolution, R_s, between lutein and zeaxanthin was 2.9. Pigments were identified on the basis of diode array spectral characteristics, retention times, and relative elution order compared to authentic standards and literature values. An examination of the diode array data from the chromatographically resolved peaks indicated that the most useful information could be obtained from monitoring the chromatographic effluent at 350, 430, and 486 nm. More carotenoid peaks were detected at 430 nm, but greater selectivity for several carotenoids was obtained at 350 and 486 nm. At 430 nm the six largest carotenoid peaks observed in orange juice consist of auroxanthin A, mutatoxanthin A, mutatoxanthin B, lutein, zeaxanthin, and isolutein. Whereas open column and thin-layer chromatography required days, the separation and identification of saponified orange juice carotenoids can now be accomplished within 40 min.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0021-8561`
A02	`01`			`@0 JAFCAU`
A03		`1`		`@0 J. agric. food chem.`
A05				`@2 44`
A06				`@2 8`
A08	`01`	`1`	`ENG`	`@1 Application of diode array detection with a C-30 reversed phase column for the separation and identification of saponified orange juice carotenoids`
A11	`01`	`1`		`@1 ROUSEFF (R.)`
A11	`02`	`1`		`@1 RALEY (L.)`
A11	`03`	`1`		`@1 HOFSOMMER (H.-J.)`
A14	`01`			`@1 Citrus Research and Education Center, University of Florida @2 Lake Alfred, Florida 33850 @3 USA @Z 1 aut. @Z 2 aut.`
A14	`02`			`@1 Gesellschaft für Lebensmittel-Forschung, Landgrafenstrasse 16 @2 10787 Berlin @3 DEU @Z 3 aut.`
A20				`@1 2176-2181`
A21				`@1 1996`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 7332 @5 354000064038940390`
A44				`@0 0000 @1 © 1996 INIST-CNRS. All rights reserved.`
A45				`@0 33 ref.`
A47	`01`	`1`		`@0 96-0438785`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Journal of agricultural and food chemistry`
A66	`01`			`@0 USA`
C01	`01`		`ENG`	@0 Thirty-nine carotenoid pigments in saponified orange (Citrus sinensis) juice were separated using a water, methanol, methyl-tert-butyl ether gradient on a non-endcapped C-30 reversed phase column. Pigments were extracted using the International Fruit Juice Union method for orange juice carotenoids, which employs precipitation with Carrez reagent and resolubilizing with acetone. Chromatographic resolution, R_s, between lutein and zeaxanthin was 2.9. Pigments were identified on the basis of diode array spectral characteristics, retention times, and relative elution order compared to authentic standards and literature values. An examination of the diode array data from the chromatographically resolved peaks indicated that the most useful information could be obtained from monitoring the chromatographic effluent at 350, 430, and 486 nm. More carotenoid peaks were detected at 430 nm, but greater selectivity for several carotenoids was obtained at 350 and 486 nm. At 430 nm the six largest carotenoid peaks observed in orange juice consist of auroxanthin A, mutatoxanthin A, mutatoxanthin B, lutein, zeaxanthin, and isolutein. Whereas open column and thin-layer chromatography required days, the separation and identification of saponified orange juice carotenoids can now be accomplished within 40 min.
C02	`01`	`X`		`@0 002A35B09`
C03	`01`	`X`	`FRE`	`@0 Cryptoxanthine @2 NK @5 01`
C03	`01`	`X`	`ENG`	`@0 Cryptoxanthin @2 NK @5 01`
C03	`01`	`X`	`SPA`	`@0 Criptoxantina @2 NK @5 01`
C03	`02`	`X`	`FRE`	`@0 Composition chimique @5 02`
C03	`02`	`X`	`ENG`	`@0 Chemical composition @5 02`
C03	`02`	`X`	`GER`	`@0 Chemische Zusammensetzung @5 02`
C03	`02`	`X`	`SPA`	`@0 Composición química @5 02`
C03	`03`	`X`	`FRE`	`@0 Pigment @5 09`
C03	`03`	`X`	`ENG`	`@0 Pigments @5 09`
C03	`03`	`X`	`GER`	`@0 Pigment @5 09`
C03	`03`	`X`	`SPA`	`@0 Pigmento @5 09`
C03	`04`	`X`	`FRE`	`@0 Méthode analytique @5 10`
C03	`04`	`X`	`ENG`	`@0 Analytical method @5 10`
C03	`04`	`X`	`SPA`	`@0 Método analítico @5 10`
C03	`05`	`X`	`FRE`	`@0 Jus d'orange @5 24`
C03	`05`	`X`	`ENG`	`@0 Orange juice @5 24`
C03	`05`	`X`	`SPA`	`@0 Zugo naranja @5 24`
C03	`06`	`X`	`FRE`	`@0 Chromatographie phase inverse @5 26`
C03	`06`	`X`	`ENG`	`@0 Reversed phase chromatography @5 26`
C03	`06`	`X`	`SPA`	`@0 Cromatografìa fase inversa @5 26`
C03	`07`	`X`	`FRE`	`@0 Séparation @5 28`
C03	`07`	`X`	`ENG`	`@0 Separation @5 28`
C03	`07`	`X`	`SPA`	`@0 Separación @5 28`
C03	`08`	`X`	`FRE`	`@0 Identification @5 29`
C03	`08`	`X`	`ENG`	`@0 Identification @5 29`
C03	`08`	`X`	`SPA`	`@0 Identificación @5 29`
C03	`09`	`X`	`FRE`	`@0 Lutéine @2 NK @5 30`
C03	`09`	`X`	`ENG`	`@0 Lutein @2 NK @5 30`
C03	`09`	`X`	`SPA`	`@0 Luteina @2 NK @5 30`
C03	`10`	`X`	`FRE`	`@0 Zéaxanthine @2 NK @5 31`
C03	`10`	`X`	`ENG`	`@0 Zeaxanthin @2 NK @5 31`
C03	`10`	`X`	`SPA`	`@0 Zeaxantina @2 NK @5 31`
C07	`01`	`X`	`FRE`	`@0 Caroténoïde @5 49`
C07	`01`	`X`	`ENG`	`@0 Carotenoid @5 49`
C07	`01`	`X`	`SPA`	`@0 Carotenoide @5 49`
N21				`@1 303`

Format Inist (serveur)

NO :	PASCAL 96-0438785 INIST
ET :	Application of diode array detection with a C-30 reversed phase column for the separation and identification of saponified orange juice carotenoids
AU :	ROUSEFF (R.); RALEY (L.); HOFSOMMER (H.-J.)
AF :	Citrus Research and Education Center, University of Florida/Lake Alfred, Florida 33850/Etats-Unis (1 aut., 2 aut.); Gesellschaft für Lebensmittel-Forschung, Landgrafenstrasse 16/10787 Berlin/Allemagne (3 aut.)
DT :	Publication en série; Niveau analytique
SO :	Journal of agricultural and food chemistry; ISSN 0021-8561; Coden JAFCAU; Etats-Unis; Da. 1996; Vol. 44; No. 8; Pp. 2176-2181; Bibl. 33 ref.
LA :	Anglais
EA :	Thirty-nine carotenoid pigments in saponified orange (Citrus sinensis) juice were separated using a water, methanol, methyl-tert-butyl ether gradient on a non-endcapped C-30 reversed phase column. Pigments were extracted using the International Fruit Juice Union method for orange juice carotenoids, which employs precipitation with Carrez reagent and resolubilizing with acetone. Chromatographic resolution, R_s, between lutein and zeaxanthin was 2.9. Pigments were identified on the basis of diode array spectral characteristics, retention times, and relative elution order compared to authentic standards and literature values. An examination of the diode array data from the chromatographically resolved peaks indicated that the most useful information could be obtained from monitoring the chromatographic effluent at 350, 430, and 486 nm. More carotenoid peaks were detected at 430 nm, but greater selectivity for several carotenoids was obtained at 350 and 486 nm. At 430 nm the six largest carotenoid peaks observed in orange juice consist of auroxanthin A, mutatoxanthin A, mutatoxanthin B, lutein, zeaxanthin, and isolutein. Whereas open column and thin-layer chromatography required days, the separation and identification of saponified orange juice carotenoids can now be accomplished within 40 min.
CC :	002A35B09
FD :	Cryptoxanthine; Composition chimique; Pigment; Méthode analytique; Jus d'orange; Chromatographie phase inverse; Séparation; Identification; Lutéine; Zéaxanthine
FG :	Caroténoïde
ED :	Cryptoxanthin; Chemical composition; Pigments; Analytical method; Orange juice; Reversed phase chromatography; Separation; Identification; Lutein; Zeaxanthin
EG :	Carotenoid
GD :	Chemische Zusammensetzung; Pigment
SD :	Criptoxantina; Composición química; Pigmento; Método analítico; Zugo naranja; Cromatografìa fase inversa; Separación; Identificación; Luteina; Zeaxantina
LO :	INIST-7332.354000064038940390
ID :	96-0438785

Links to Exploration step

Pascal:96-0438785

Le document en format XML

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<term>Pigments</term>
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<term>Separation</term>
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<term>Chromatographie phase inverse</term>
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<front><div type="abstract" xml:lang="en">Thirty-nine carotenoid pigments in saponified orange (Citrus sinensis) juice were separated using a water, methanol, methyl-tert-butyl ether gradient on a non-endcapped C-30 reversed phase column. Pigments were extracted using the International Fruit Juice Union method for orange juice carotenoids, which employs precipitation with Carrez reagent and resolubilizing with acetone. Chromatographic resolution, R<sub>s</sub>
, between lutein and zeaxanthin was 2.9. Pigments were identified on the basis of diode array spectral characteristics, retention times, and relative elution order compared to authentic standards and literature values. An examination of the diode array data from the chromatographically resolved peaks indicated that the most useful information could be obtained from monitoring the chromatographic effluent at 350, 430, and 486 nm. More carotenoid peaks were detected at 430 nm, but greater selectivity for several carotenoids was obtained at 350 and 486 nm. At 430 nm the six largest carotenoid peaks observed in orange juice consist of auroxanthin A, mutatoxanthin A, mutatoxanthin B, lutein, zeaxanthin, and isolutein. Whereas open column and thin-layer chromatography required days, the separation and identification of saponified orange juice carotenoids can now be accomplished within 40 min.</div>
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<sZ>2 aut.</sZ>
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<fC01 i1="01" l="ENG"><s0>Thirty-nine carotenoid pigments in saponified orange (Citrus sinensis) juice were separated using a water, methanol, methyl-tert-butyl ether gradient on a non-endcapped C-30 reversed phase column. Pigments were extracted using the International Fruit Juice Union method for orange juice carotenoids, which employs precipitation with Carrez reagent and resolubilizing with acetone. Chromatographic resolution, R<sub>s</sub>
, between lutein and zeaxanthin was 2.9. Pigments were identified on the basis of diode array spectral characteristics, retention times, and relative elution order compared to authentic standards and literature values. An examination of the diode array data from the chromatographically resolved peaks indicated that the most useful information could be obtained from monitoring the chromatographic effluent at 350, 430, and 486 nm. More carotenoid peaks were detected at 430 nm, but greater selectivity for several carotenoids was obtained at 350 and 486 nm. At 430 nm the six largest carotenoid peaks observed in orange juice consist of auroxanthin A, mutatoxanthin A, mutatoxanthin B, lutein, zeaxanthin, and isolutein. Whereas open column and thin-layer chromatography required days, the separation and identification of saponified orange juice carotenoids can now be accomplished within 40 min.</s0>
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<fC03 i1="01" i2="X" l="FRE"><s0>Cryptoxanthine</s0>
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<s2>NK</s2>
<s5>01</s5>
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<s5>02</s5>
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<fC03 i1="02" i2="X" l="SPA"><s0>Composición química</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Pigment</s0>
<s5>09</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Pigments</s0>
<s5>09</s5>
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<fC03 i1="03" i2="X" l="GER"><s0>Pigment</s0>
<s5>09</s5>
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<s5>09</s5>
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<s5>10</s5>
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<s5>10</s5>
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<fC03 i1="05" i2="X" l="ENG"><s0>Orange juice</s0>
<s5>24</s5>
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<s5>24</s5>
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<s5>26</s5>
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<s5>26</s5>
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<s5>28</s5>
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<s5>28</s5>
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<s2>NK</s2>
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<s2>NK</s2>
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<s5>31</s5>
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<fC07 i1="01" i2="X" l="ENG"><s0>Carotenoid</s0>
<s5>49</s5>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Carotenoide</s0>
<s5>49</s5>
</fC07>
<fN21><s1>303</s1>
</fN21>
</pA>
</standard>
<server><NO>PASCAL 96-0438785 INIST</NO>
<ET>Application of diode array detection with a C-30 reversed phase column for the separation and identification of saponified orange juice carotenoids</ET>
<AU>ROUSEFF (R.); RALEY (L.); HOFSOMMER (H.-J.)</AU>
<AF>Citrus Research and Education Center, University of Florida/Lake Alfred, Florida 33850/Etats-Unis (1 aut., 2 aut.); Gesellschaft für Lebensmittel-Forschung, Landgrafenstrasse 16/10787 Berlin/Allemagne (3 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of agricultural and food chemistry; ISSN 0021-8561; Coden JAFCAU; Etats-Unis; Da. 1996; Vol. 44; No. 8; Pp. 2176-2181; Bibl. 33 ref.</SO>
<LA>Anglais</LA>
<EA>Thirty-nine carotenoid pigments in saponified orange (Citrus sinensis) juice were separated using a water, methanol, methyl-tert-butyl ether gradient on a non-endcapped C-30 reversed phase column. Pigments were extracted using the International Fruit Juice Union method for orange juice carotenoids, which employs precipitation with Carrez reagent and resolubilizing with acetone. Chromatographic resolution, R<sub>s</sub>
, between lutein and zeaxanthin was 2.9. Pigments were identified on the basis of diode array spectral characteristics, retention times, and relative elution order compared to authentic standards and literature values. An examination of the diode array data from the chromatographically resolved peaks indicated that the most useful information could be obtained from monitoring the chromatographic effluent at 350, 430, and 486 nm. More carotenoid peaks were detected at 430 nm, but greater selectivity for several carotenoids was obtained at 350 and 486 nm. At 430 nm the six largest carotenoid peaks observed in orange juice consist of auroxanthin A, mutatoxanthin A, mutatoxanthin B, lutein, zeaxanthin, and isolutein. Whereas open column and thin-layer chromatography required days, the separation and identification of saponified orange juice carotenoids can now be accomplished within 40 min.</EA>
<CC>002A35B09</CC>
<FD>Cryptoxanthine; Composition chimique; Pigment; Méthode analytique; Jus d'orange; Chromatographie phase inverse; Séparation; Identification; Lutéine; Zéaxanthine</FD>
<FG>Caroténoïde</FG>
<ED>Cryptoxanthin; Chemical composition; Pigments; Analytical method; Orange juice; Reversed phase chromatography; Separation; Identification; Lutein; Zeaxanthin</ED>
<EG>Carotenoid</EG>
<GD>Chemische Zusammensetzung; Pigment</GD>
<SD>Criptoxantina; Composición química; Pigmento; Método analítico; Zugo naranja; Cromatografìa fase inversa; Separación; Identificación; Luteina; Zeaxantina</SD>
<LO>INIST-7332.354000064038940390</LO>
<ID>96-0438785</ID>
</server>
</inist>
</record>

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Application of diode array detection with a C-30 reversed phase column for the separation and identification of saponified orange juice carotenoids

Application of diode array detection with a C-30 reversed phase column for the separation and identification of saponified orange juice carotenoids

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