Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pra
Identifieur interne : 002344 ( Main/Exploration ); précédent : 002343; suivant : 002345Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pra
Auteurs : Rbia P. Purcino [Brésil] ; Camilo Lzaro Medina [Brésil] ; Daniel Martins De Souza [Brésil] ; Flvia Vischi Winck [Brésil] ; Eduardo Caruso Machado [Brésil] ; Jos Camilo Novello [Brésil] ; Marcos Antonio Machado [Brésil] ; Paulo Mazzafera [Brésil]Source :
- Journal of Experimental Botany [ 0022-0957 ] ; 2007-07-03.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
- Amino Acids (metabolism), Aminoacid, Chlorosis, Citrus sinensis, Citrus sinensis (enzymology), Citrus sinensis (metabolism), Citrus sinensis (microbiology), Electrophoresis, Gel, Two-Dimensional, Metabolism, Nitrate reductase, Nitrates (metabolism), Nitrogen (metabolism), Peptidases, Plant Diseases, Plant Leaves (metabolism), Plant Leaves (microbiology), Plant Proteins (metabolism), Polyamine, Polyamines (metabolism), Proteome, Stress, Xylella (physiology), Xylem, Xylem (metabolism), Xylem (microbiology).
- MESH :
- chemical , metabolism : Amino Acids, Nitrates, Nitrogen, Plant Proteins, Polyamines.
- enzymology : Citrus sinensis.
- metabolism : Citrus sinensis, Plant Leaves, Xylem.
- microbiology : Citrus sinensis, Plant Leaves, Xylem.
- physiology : Xylella.
- Electrophoresis, Gel, Two-Dimensional, Plant Diseases, Proteome.
Abstract
Xylella fastidiosa (Xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (Citrus sinensis L. Osb.) causing Pierce disease and citrus variegated chlorosis (CVC), respectively. The aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. Pra (C. sinensis L. Osbeck) infected with Xf. Plants were artificially infected and maintained in the greenhouse until they have developed clear disease symptoms. The content of nitrogen compounds and enzymes of the nitrogen metabolism and proteases in the xylem sap and leaves of diseased (DP) and uninfected healthy (HP) plants was studied. The activity of nitrate reductase in leaves did not change in DP, however, the activity of glutamine synthetase was significantly higher in these leaves. Although amino acid concentration was slightly higher in the xylem sap of DP, the level dropped drastically in the leaves. The protein contents were lower in the sap and in leaves of DP. DP and HP showed the same amino acid profiles, but different proportions were observed among them, mainly for asparagine, glutamine, and arginine. The polyamine putrescine was found in high concentrations only in DP. Protease activity was higher in leaves of DP while, in the xylem sap, activity was detected only in DP. Bidimensional electrophoresis showed a marked change in the protein pattern in DP. Five differentially expressed proteins were identified (2 from HP and 3 from DP), but none showed similarity with the genomic (translated) and proteomic database of Xf, but do show similarity with the proteins thaumatin, mucin, peroxidase, ABC-transporter, and strictosidine synthase. These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant.
Url:
DOI: 10.1093/jxb/erm138
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000903
- to stream Istex, to step Curation: 000903
- to stream Istex, to step Checkpoint: 000622
- to stream PubMed, to step Corpus: 000B79
- to stream PubMed, to step Curation: 000B79
- to stream PubMed, to step Checkpoint: 000B79
- to stream Ncbi, to step Merge: 000718
- to stream Ncbi, to step Curation: 000718
- to stream Ncbi, to step Checkpoint: 000718
- to stream Main, to step Merge: 002428
- to stream PascalFrancis, to step Corpus: 000435
- to stream PascalFrancis, to step Curation: 000569
- to stream PascalFrancis, to step Checkpoint: 000417
- to stream Main, to step Merge: 002507
- to stream Main, to step Curation: 002344
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pra</title>
<author><name sortKey="Purcino, Rbia P" sort="Purcino, Rbia P" uniqKey="Purcino R" first="Rbia P." last="Purcino">Rbia P. Purcino</name>
</author>
<author><name sortKey="Medina, Camilo Lzaro" sort="Medina, Camilo Lzaro" uniqKey="Medina C" first="Camilo Lzaro" last="Medina">Camilo Lzaro Medina</name>
</author>
<author><name sortKey="De Souza, Daniel Martins" sort="De Souza, Daniel Martins" uniqKey="De Souza D" first="Daniel Martins" last="De Souza">Daniel Martins De Souza</name>
</author>
<author><name sortKey="Winck, Flvia Vischi" sort="Winck, Flvia Vischi" uniqKey="Winck F" first="Flvia Vischi" last="Winck">Flvia Vischi Winck</name>
</author>
<author><name sortKey="Machado, Eduardo Caruso" sort="Machado, Eduardo Caruso" uniqKey="Machado E" first="Eduardo Caruso" last="Machado">Eduardo Caruso Machado</name>
</author>
<author><name sortKey="Novello, Jos Camilo" sort="Novello, Jos Camilo" uniqKey="Novello J" first="Jos Camilo" last="Novello">Jos Camilo Novello</name>
</author>
<author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
</author>
<author><name sortKey="Mazzafera, Paulo" sort="Mazzafera, Paulo" uniqKey="Mazzafera P" first="Paulo" last="Mazzafera">Paulo Mazzafera</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:61E10CB70513F05461A1B27DE6B2ADC6D2513F3A</idno>
<date when="2007" year="2007">2007</date>
<idno type="doi">10.1093/jxb/erm138</idno>
<idno type="url">https://api.istex.fr/document/61E10CB70513F05461A1B27DE6B2ADC6D2513F3A/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000903</idno>
<idno type="wicri:Area/Istex/Curation">000903</idno>
<idno type="wicri:Area/Istex/Checkpoint">000622</idno>
<idno type="wicri:doubleKey">0022-0957:2007:Purcino R:xylella:fastidiosa:disturbs</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:17609534</idno>
<idno type="wicri:Area/PubMed/Corpus">000B79</idno>
<idno type="wicri:Area/PubMed/Curation">000B79</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000B79</idno>
<idno type="wicri:Area/Ncbi/Merge">000718</idno>
<idno type="wicri:Area/Ncbi/Curation">000718</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000718</idno>
<idno type="wicri:doubleKey">0022-0957:2007:Purcino R:xylella:fastidiosa:disturbs</idno>
<idno type="wicri:Area/Main/Merge">002428</idno>
<idno type="wicri:source">INIST</idno>
<idno type="RBID">Pascal:08-0012589</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000435</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000569</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000417</idno>
<idno type="wicri:doubleKey">0022-0957:2007:Purcino R:xylella:fastidiosa:disturbs</idno>
<idno type="wicri:Area/Main/Merge">002507</idno>
<idno type="wicri:Area/Main/Curation">002344</idno>
<idno type="wicri:Area/Main/Exploration">002344</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pra</title>
<author><name sortKey="Purcino, Rbia P" sort="Purcino, Rbia P" uniqKey="Purcino R" first="Rbia P." last="Purcino">Rbia P. Purcino</name>
<affiliation wicri:level="2"><country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Fisiologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, 13083-970, Campinas, SP</wicri:regionArea>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
<affiliation wicri:level="2"><country xml:lang="fr">Brésil</country>
<wicri:regionArea>Universidade Federal Rural do Rio de Janeiro, Km 7 Rodovia BR 465, 23850-000, Seropdica, RJ</wicri:regionArea>
<placeName><region type="state">État de Rio de Janeiro</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Medina, Camilo Lzaro" sort="Medina, Camilo Lzaro" uniqKey="Medina C" first="Camilo Lzaro" last="Medina">Camilo Lzaro Medina</name>
<affiliation wicri:level="2"><country xml:lang="fr">Brésil</country>
<wicri:regionArea>Conplant, Rua Francisco Andreo Aledo, 22, 13084-200, Campinas, SP</wicri:regionArea>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="De Souza, Daniel Martins" sort="De Souza, Daniel Martins" uniqKey="De Souza D" first="Daniel Martins" last="De Souza">Daniel Martins De Souza</name>
<affiliation wicri:level="2"><country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Bioqumica, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, 13083-970, Campinas, SP</wicri:regionArea>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Winck, Flvia Vischi" sort="Winck, Flvia Vischi" uniqKey="Winck F" first="Flvia Vischi" last="Winck">Flvia Vischi Winck</name>
<affiliation wicri:level="2"><country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Bioqumica, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, 13083-970, Campinas, SP</wicri:regionArea>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Machado, Eduardo Caruso" sort="Machado, Eduardo Caruso" uniqKey="Machado E" first="Eduardo Caruso" last="Machado">Eduardo Caruso Machado</name>
<affiliation wicri:level="2"><country xml:lang="fr">Brésil</country>
<wicri:regionArea>Instituto Agronmico de Campinas, Centro de Ecofisiologia e Biofsica, Av. Baro de Itapura 1481, CP 28, 13012-970, Campinas, SP</wicri:regionArea>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Novello, Jos Camilo" sort="Novello, Jos Camilo" uniqKey="Novello J" first="Jos Camilo" last="Novello">Jos Camilo Novello</name>
<affiliation wicri:level="2"><country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Bioqumica, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, 13083-970, Campinas, SP</wicri:regionArea>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
<affiliation wicri:level="2"><country xml:lang="fr">Brésil</country>
<wicri:regionArea>Instituto Agronmico de Campinas, Centro APTA Citros Sylvio Moreira, CP 04, 13490-970, Cordeirpolis, SP</wicri:regionArea>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mazzafera, Paulo" sort="Mazzafera, Paulo" uniqKey="Mazzafera P" first="Paulo" last="Mazzafera">Paulo Mazzafera</name>
<affiliation wicri:level="2"><country xml:lang="fr">Brésil</country>
<wicri:regionArea>Departamento de Fisiologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, 13083-970, Campinas, SP</wicri:regionArea>
<placeName><region type="state">État de São Paulo</region>
</placeName>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Brésil</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Journal of Experimental Botany</title>
<idno type="ISSN">0022-0957</idno>
<idno type="eISSN">1460-2431</idno>
<imprint><publisher>Oxford University Press</publisher>
<date type="published" when="2007-07-03">2007-07-03</date>
<biblScope unit="volume">58</biblScope>
<biblScope unit="issue">11</biblScope>
<biblScope unit="page" from="2733">2733</biblScope>
<biblScope unit="page" to="2744">2744</biblScope>
</imprint>
<idno type="ISSN">0022-0957</idno>
</series>
<idno type="istex">61E10CB70513F05461A1B27DE6B2ADC6D2513F3A</idno>
<idno type="DOI">10.1093/jxb/erm138</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0022-0957</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acids (metabolism)</term>
<term>Aminoacid</term>
<term>Chlorosis</term>
<term>Citrus sinensis</term>
<term>Citrus sinensis (enzymology)</term>
<term>Citrus sinensis (metabolism)</term>
<term>Citrus sinensis (microbiology)</term>
<term>Electrophoresis, Gel, Two-Dimensional</term>
<term>Metabolism</term>
<term>Nitrate reductase</term>
<term>Nitrates (metabolism)</term>
<term>Nitrogen (metabolism)</term>
<term>Peptidases</term>
<term>Plant Diseases</term>
<term>Plant Leaves (metabolism)</term>
<term>Plant Leaves (microbiology)</term>
<term>Plant Proteins (metabolism)</term>
<term>Polyamine</term>
<term>Polyamines (metabolism)</term>
<term>Proteome</term>
<term>Stress</term>
<term>Xylella (physiology)</term>
<term>Xylem</term>
<term>Xylem (metabolism)</term>
<term>Xylem (microbiology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Amino Acids</term>
<term>Nitrates</term>
<term>Nitrogen</term>
<term>Plant Proteins</term>
<term>Polyamines</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus sinensis</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus sinensis</term>
<term>Plant Leaves</term>
<term>Xylem</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus sinensis</term>
<term>Plant Leaves</term>
<term>Xylem</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Xylella</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Electrophoresis, Gel, Two-Dimensional</term>
<term>Plant Diseases</term>
<term>Proteome</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Aminoacide</term>
<term>Chlorose</term>
<term>Citrus sinensis</term>
<term>Métabolisme</term>
<term>Nitrate reductase</term>
<term>Peptidases</term>
<term>Polyamine</term>
<term>Stress</term>
<term>Xylème</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract">Xylella fastidiosa (Xf) is a fastidious bacterium that grows exclusively in the xylem of several important crop species, including grape and sweet orange (Citrus sinensis L. Osb.) causing Pierce disease and citrus variegated chlorosis (CVC), respectively. The aim of this work was to study the nitrogen metabolism of a highly susceptible variety of sweet orange cv. Pra (C. sinensis L. Osbeck) infected with Xf. Plants were artificially infected and maintained in the greenhouse until they have developed clear disease symptoms. The content of nitrogen compounds and enzymes of the nitrogen metabolism and proteases in the xylem sap and leaves of diseased (DP) and uninfected healthy (HP) plants was studied. The activity of nitrate reductase in leaves did not change in DP, however, the activity of glutamine synthetase was significantly higher in these leaves. Although amino acid concentration was slightly higher in the xylem sap of DP, the level dropped drastically in the leaves. The protein contents were lower in the sap and in leaves of DP. DP and HP showed the same amino acid profiles, but different proportions were observed among them, mainly for asparagine, glutamine, and arginine. The polyamine putrescine was found in high concentrations only in DP. Protease activity was higher in leaves of DP while, in the xylem sap, activity was detected only in DP. Bidimensional electrophoresis showed a marked change in the protein pattern in DP. Five differentially expressed proteins were identified (2 from HP and 3 from DP), but none showed similarity with the genomic (translated) and proteomic database of Xf, but do show similarity with the proteins thaumatin, mucin, peroxidase, ABC-transporter, and strictosidine synthase. These results showed that significant changes take place in the nitrogen metabolism of DP, probably as a response to the alterations in the absorption, assimilation and distribution of N in the plant.</div>
</front>
</TEI>
<affiliations><list><country><li>Brésil</li>
</country>
<region><li>État de Rio de Janeiro</li>
<li>État de São Paulo</li>
</region>
</list>
<tree><country name="Brésil"><region name="État de São Paulo"><name sortKey="Purcino, Rbia P" sort="Purcino, Rbia P" uniqKey="Purcino R" first="Rbia P." last="Purcino">Rbia P. Purcino</name>
</region>
<name sortKey="De Souza, Daniel Martins" sort="De Souza, Daniel Martins" uniqKey="De Souza D" first="Daniel Martins" last="De Souza">Daniel Martins De Souza</name>
<name sortKey="Machado, Eduardo Caruso" sort="Machado, Eduardo Caruso" uniqKey="Machado E" first="Eduardo Caruso" last="Machado">Eduardo Caruso Machado</name>
<name sortKey="Machado, Marcos Antonio" sort="Machado, Marcos Antonio" uniqKey="Machado M" first="Marcos Antonio" last="Machado">Marcos Antonio Machado</name>
<name sortKey="Mazzafera, Paulo" sort="Mazzafera, Paulo" uniqKey="Mazzafera P" first="Paulo" last="Mazzafera">Paulo Mazzafera</name>
<name sortKey="Mazzafera, Paulo" sort="Mazzafera, Paulo" uniqKey="Mazzafera P" first="Paulo" last="Mazzafera">Paulo Mazzafera</name>
<name sortKey="Medina, Camilo Lzaro" sort="Medina, Camilo Lzaro" uniqKey="Medina C" first="Camilo Lzaro" last="Medina">Camilo Lzaro Medina</name>
<name sortKey="Novello, Jos Camilo" sort="Novello, Jos Camilo" uniqKey="Novello J" first="Jos Camilo" last="Novello">Jos Camilo Novello</name>
<name sortKey="Purcino, Rbia P" sort="Purcino, Rbia P" uniqKey="Purcino R" first="Rbia P." last="Purcino">Rbia P. Purcino</name>
<name sortKey="Winck, Flvia Vischi" sort="Winck, Flvia Vischi" uniqKey="Winck F" first="Flvia Vischi" last="Winck">Flvia Vischi Winck</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002344 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002344 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Bois |area= OrangerV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:61E10CB70513F05461A1B27DE6B2ADC6D2513F3A |texte= Xylella fastidiosa disturbs nitrogen metabolism and causes a stress response in sweet orange Citrus sinensis cv. Pra }}
![]() | This area was generated with Dilib version V0.6.25. | ![]() |